Blue lupin (Lupinus angustifolius L.) ‒ the results of varieties and accessions study according to adaptivity and a set of commercialbiological traits

Cultivation of grain legumes including blue lupin (Lupinus angustifolius L.) is an important reserve for increase of high protein feed production. The aim of the research is to find perspective blue lupin varieties and accessions according to yield, adaptivity, the duration of vegetation period and biochemical indices in south-western area of the Central part of Russia. In 2019-2021 six blue lupin varieties and four accessions developed in the All-Russian Lupin Scientific Research Institute have been tested in competitive variety trial (Vityas was standard variety). The trials were laid on sod-podzolic, loamy soil with the moderate fertility level. The average grain yield of varieties in the trial was 2.07 t/ha, of standard variety – 1.85 t/ha. The varieties Bryansky kormovoy, Uzkolistny 53 and accessions BSv 51-19, USN 53-236 and SBS 56-15 had the highest grain yield. Their grain productivity varied from 2.17 to 2.29 t/ha. Statistically significant increase to the standard was 0.32-0.44 t/ha. The accessions BSv 51-19 and USN 53-236 had the maximum grain yield by adaptivity coefficient of 110%. According to the green mass yield and adaptivity Bryansky kormovoy and new promising accessions SBS 56-15, USN 53-236 and BSv 51-19 have been noted: the increase to the standard according to the green mass yield was 5.0-7.0 t/ha, the adaptivity coefficient was 104-110%. By the duration of vegetation period (80-89 days) all varieties and accessions were included into the group of early-ripening. The new tall variety Belorozovy 144 had the longest vegetation period (89 days). According to the alkaloid content in the grain (0.031-0.063 %) all tested varieties and accessions were included into the low-alkaloid group. The varieties Smena, Uzkolistny 53 and the accession SBS 56-15 were characterized by stable low index (0.031-0.039 %). The content of raw protein in the seeds of tested varieties and accessions of blue lupin varied in the range of 32.0-33.8%. According to the set of positive traits, Bryansky kormovoy variety and accessions USN 53-236 and SBS 56-15 have been noted

Analysis of Emergency Situations When Working with Pathogenic Biological Agents within the Framework of Risk-Oriented Approach

The aim – based on the analysis of accidents when working with pathogenic biological agents (PBA) of pathogenicity groups I–II, draw conclusions about the causes of their occurrence and formulate recommendations for improving biological safety measures to reduce the risk of accidents.Materials and methods. The subject of the study was the data on accidents that happened during the work with PBA, stated in protocols of the commission for monitoring compliance with biological safety requirements of the Volgograd Research Anti-Plague Institute over the period of 1986–2020. Assessed were the type of emergency, their number, main causes and prerequisites for occurrence, professional category of a worker who participated in an accident.Results and discussion. During the specified period 3 types of accidents were recorded: accidents with spraying, accidents with skin lesion, accidents without spraying. There were no accidents with damage to the insulating suit and the pneumatic suit during the entire period under investigation. Of the total number of accidents, 42.85 % of cases were associated with skin lesion due to the bite of an experimental animal due to its incorrect fixation during infection, feeding, care, or due to autopsy of animals. Spillage accidents were recorded in 42.85 %; accidents without spraying amounted to 14.2 %. The categories of employees who made the greatest number of accidents have been identified: laboratory assistants – 39.2 % of cases, researcher officers – 14.2 %, disinfectors – 14.2 %. The causes of accidents and the prerequisites contributing to their realization have been pinpointed. The main ways and measures to reduce the risks of emergency situations for personnel when working with pathogens of particularly dangerous infectious diseases are put forward

The use of <i>in vitro</i> androgenesis for the involvement of interspecific hybrids between <i>Solanum tuberosum</i> L. and wild allotetraploid potato species <i>Solanum stoloniferum</i> Schltdl. et Bouché into breeding

Wild allotetraploid potato species Solanum stoloniferum Schltdl. &amp; Bouché from Mexico is regarded as a valuable source of resistance genes for use in breeding. However, introgression of its resistance genes into breeding material is hampered by a set of reproductive barriers. The genomic difference between S. stoloniferum (genome ААВВ) and S. tuberosum L. (AAAA) is one of them. This makes questionable the possibility of transferring a variety of valuable genes of the wild species localized on the chromosomes of its genome B into the genome of cultivated potatoes. It is proposed to produce tetraploid (4x, AAAB) interspecific hybrids of S. stoloniferum, which are regarded as more promising for homoeological recombination than pentaploid (5x, ААААВ) hybrids commonly used in the introgression schemes. However, the effective ploidy of tetraploid hybrids (3EBN) hinders their backcrossing to cultivated potatoes (4 EBN). For instance, our attempts to involve the tetraploid hybrid of S. stoloniferum IGC16/36.1 obtained by us into hybridization with potato varieties were unsuccessful for a number of years. To solve this problem, we suggested a technique based on the production of 4x plants obtained in anther culture of this hybrid. The present research was aimed at assessing the efficiency of this approach.Thirty-one plants were obtained in anther culture (androgenic clones, androclones) of the hybrid IGC16/36.1 in 2018. Most of them exceeded the initial hybrid in habitus strength and flowering intensity. As a result of crosses made in 2019, 1039 hybrid seeds were obtained from crossing 21 androclones with the ‘Lemhi Russet’ variety (8.7 seeds/pollination), 1017 seeds (7.5 seeds/pollination) from crosses of 23 androclones with the ‘Quarta’ variety, and 716 seeds (12.3 seeds/pollination) from crosses of 11 androclones and a diploid potato line IGC 17n8 capable of producing fertile unreduced (2n) pollen. The hybrid seeds had good germination rate of 70-90%. Among the androclones that gave progeny in crosses with potato varieties, we identified genotypes carrying DNA markers of late blight (LB) resistance genes Rpi-sto1, R2 and R3b, PVY resistance genes Ryadg, Rysto and Rychc, and potato wart disease resistance gene Sen2 (these markers were found in the initial accession of S. stoloniferum PI 205522 and in the IGC 16/36.1 hybrid). Despite the complex nature of inheritance of the analyzed markers in progenies of backcrosses of androclones, a number of isolated hybrids carried several markers, including those of the Rpi-sto1, a broad-spectrum gene for high resistance to late blight. Hybrids with relatively high tuber productivity, features of cultivated potatoes such as regularly shaped tubers with small eyes, and high field resistance to late blight were selected.The prospects for using androclones of the tetraploid interspecific hybrid IGC 16/36.1 for increasing the frequency of homoeologous A/B recombination of chromosomes are discussed

A Review of the Properties of Nb3Sn and Their Variation with A15 Composition, Morphology and Strain State

This article gives an overview of the available literature on simplified, well defined (quasi-)homogeneous laboratory samples. After more than 50 years of research on superconductivity in Nb3Sn, a significant amount of results are available, but these are scattered over a multitude of publications. Two reviews exist on the basic properties of A15 materials in general, but no specific review for Nb3Sn is available. This article is intended to provide such an overview. It starts with a basic description of the Niobium-Tin intermetallic. After this it maps the influence of Sn content on the the electron-phonon interaction strength and on the field-temperature phase boundary. The literature on the influence of Cu, Ti and Ta additions will then be briefly summarized. This is followed by a review on the effects of grain size and strain. The article is concluded with a summary of the main results.Comment: Invited Topical Review for Superconductor, Science and Technology. Provisionally scheduled for July 200

Peculiarities of Burkholderia mallei and Burkholderia pseudomallei Identification Using Microbiological Analyzer Vitek 2 Compact 30

Automated Vitek 2 system, based on comparison of a biochemical profile of the studied bacterial cultures with the existing database, is widely used for B. pseudomallei and B. mallei identification in the laboratory practice.Objective of the study is to conduct extended phenotypic characterization of the strains of glanders and melioidosis causative agents, stored in the biobank of the Volgograd Research Anti-Plague Institute and analyze variations in their biochemical profiles, using Vitek 2 system.Materials and methods. Using Vitek 2 device, (bioMerieux, France) analyzed have been biochemical properties of 52 collection strains of B. pseudomallei and B. mallei grown on L-agar (Difco, USA) and trypticase-soy agar – TSA (HiMedia, India).Results and discussion. Most of the investigated strains (31 out of 40 B. pseudomallei and 8 of 12 B. mallei) have been identified with an acceptable probability for determining certain specie appurtenance, amounting to 90–99 %. The percentage of correct identification of B. pseudomallei and B. mallei is higher when strains are cultured on L-agar, than when on TSA. Due to the variability of the biochemical features, some strains have showed non-typical for its species results in certain tests (for B. pseudomallei strains – the absence of enzyme activity of β-N-acetyl-glucosaminidase, β-N-acetyl-galactosaminidase and ability to utilize D-cellobiose; for B. mallei strains – the absence of enzyme activity of L-proline-aryl-amidase and tyrosin-aryl-amidase, existence of glycin-aryl-amidase activity and ability to utilize sucrose, D-trehalose), which has led to their mal-identification. The probability of error diagnostics of microorganisms belonging to Burkholderia species necessitates up-dating of the database built into Vitek 2 analyzer as regards biochemical characteristics of the strains which have peculiar profiles

Интегральная работа внутрилегочного источника механической энергии у здоровых людей и при обструктивных заболеваниях легких

Two functional levels of mechanical energy intrapulmonary source have been viewed in this article for the first time. The first level is located into the perialveolar space. This level provides inspiratory and expiratory activity of the lungs together with respiratory muscles. Due to this, the inspiratory alveolar pressure is lower than the intrapleural pressure and the expiratory alveolar pressure is higher than the intrapleural pressure. This is deter mined by work of breathing measured as negative elastic hysteresis area and allows overcoming some nonelastic resistance of the lungs. The lung activity on overcoming the elastic resistance is estimated by multiplying the total work of breathing by a functional coefficient of lung elasticity (FCLE). The latter is calculated by dividing the total lung compliance by the dynamic lung compliance and reflects change in the elastic lung tension from spontaneous breathing level to the total lung compliance level. FCLE approximates 1 in healthy subjects and in patients with normal lung elastic recoil (LER). LER is lower and the total lung capacity is higher in lung obstructive diseases. Consequently, the total lung compliance could increase and FCLE could grow up to 10 in such cases (for instance, in severe emphysema). This leads to increasing lung activity on overcoming the elastic resistance up to 10 kgf × m / min or higher. Actually, this huge work of breathing is not performed due to the 2nd level of lung mechanical activity that is an active dilation of the large airways on expiration; this mechanism prevents valvular bronchial obstruction.Впервые рассматриваются 2 уровня функционирования внутрилегочного источника механической энергии: 1й находится в периальвеолярных структурах легких и помимо дыхательной мускулатуры обеспечивает инспираторное и экспираторное действие легких. В силу этого альвеолярное давление на вдохе ниже внутриплеврального, а на выдохе – выше внутриплеврального. Это способствует преодолению части неэластического сопротивления легких и определяется величиной работы дыхания, измеренной по площади отрицательного эластического гистерезиса легких. Работа самих легких по преодолению эластического сопротивления легких рассчитывается путем умножения показателей общей работы дыхания и функционального коэффициента эластичности легких (ФКЭЛ). Он определяется делением значения общей растяжимости легких (ОРЛ) на показатель динамической растяжимости легких и означает степень изменения эластического напряжения легких от уровня спонтанного дыхания до величины ОРЛ. У здоровых людей и пациентов с нормальной эластической тягой легких (ЭТЛ) ФКЭЛ близок к единице. При обструктивных нарушениях вентиляционной функции ЭТЛ снижается, увеличивается общая емкость легких. Соответственно увеличивается ОРЛ и поэтому ФКЭЛ может возрастать до 10 (при резко выраженной эмфиземе легких). В таком случае работа по преодолению эластического сопротивления легких может достигать ≥ 10 кгм / мин. В действительности эта гигантская работа дыхания не совершается благодаря 2му уровню механической активности легких – активному расширению крупных бронхов на выдохе, противодействующему клапанной обструкции бронхов

РАДИОНУКЛИДНАЯ ОЦЕНКА АЛЬВЕОЛЯРНО КАПИЛЛЯРНОЙ ПРОНИЦАЕМОСТИ ДЛЯ ДИФФЕРЕНЦИАЛЬНОЙ ДИАГНОСТИКИ ЛЕГОЧНОГО ИНФИЛЬТРАТА

Summary.Ventilation scintigraphy of the lungs with analysis of alveolar-capillary permeability (ACP) was performed in patients with confirmed diagnoses of community-acquired pneumonia (CAP, n = 40), infiltrative pulmonary tuberculosis (IPT, n = 20), distal pulmonary embolism (DPE, n = 11),peripheral lung carcinoma (PLC, n = 17), and in 22 healthy volunteers. ACP values were higher in affected and intact lungs in patients with CAP at 10 and 30 min of the investigation vs those with DPE and were higher at 30 min in the affected lung in patients with CAP compared to patients with IPT. Comparison of ACPs in DPE and IPT patients revealed the ACP reduction in affected and intact lungs of DPE patients at 30 min of the investigation. PLC patients demonstrated the ACP reduction in affected lung compared to contralateral lung. Therefore, ACP gave additional information for differential diagnosis of CAP, IPT, DPE and PLCРезюме. У 88 пациентов с верифицированными диагнозами: внебольничная пневмония (ВП) – 40, инфильтративный туберкулез легких(ИТЛ) – 20, тромбоэмболия дистальных ветвей легочной артерии (ТДВЛА) – 11, периферический рак легких (ПРЛ) – 17, а также у 22 здоровых добровольцев проведена вентиляционная пульмоносцинтиграфия с анализом альвеолярно-капиллярной проницаемости (АКП). При ВП, в отличие от ТДВЛА и ИТЛ, АКП была выше на 10-й и 30-й мин исследования в пораженном и интактном легких по сравнению с ТДВЛА и на 30-й мин исследования – в пораженном легком по сравнению с ИТЛ. Сопоставление значений АКП у больных ТДВЛА и ИТЛ показало снижение АКП на 30-й мин исследования у больных ТДВЛА в пораженном и интактном легких. У пациентов с ПРЛ регистрировалось снижение АКП на стороне поражения по сравнению с контралатеральным легким. Таким образом, оценка АКП позволяет расширить возможности дифференциальной диагностики ВП, ИТЛ, ТДВЛА и ПРЛ

Decrypting magnetic fabrics (AMS, AARM, AIRM) through the analysis of mineral shape fabrics and distribution anisotropy

The fieldwork was supported by the DIPS project (grant no. 240467) and the MIMES project (grant no. 244155) funded by the Norwegian Research Council awarded to O.G. O.P.'s position was funded from Y-TEC.Anisotropy of magnetic susceptibility (AMS) and anisotropy of magnetic remanence (AARM and AIRM) are efficient and versatile techniques to indirectly determine rock fabrics. Yet, deciphering the source of a magnetic fabric remains a crucial and challenging step, notably in the presence of ferrimagnetic phases. Here we use X-ray micro-computed tomography to directly compare mineral shape-preferred orientation and spatial distribution fabrics to AMS, AARM and AIRM fabrics from five hypabyssal trachyandesite samples. Magnetite grains in the trachyandesite are euhedral with a mean aspect ratio of 1.44 (0.24 s.d., long/short axis), and > 50% of the magnetite grains occur in clusters, and they are therefore prone to interact magnetically. Amphibole grains are prolate with magnetite in breakdown rims. We identified three components of the petrofabric that influence the AMS of the analyzed samples: the magnetite and the amphibole shape fabrics and the magnetite spatial distribution. Depending on their relative strength, orientation and shape, these three components interfere either constructively or destructively to produce the AMS fabric. If the three components are coaxial, the result is a relatively strongly anisotropic AMS fabric (P’ = 1.079). If shape fabrics and/or magnetite distribution are non-coaxial, the resulting AMS is weakly anisotropic (P’ = 1.012). This study thus reports quantitative petrofabric data that show the effect of magnetite distribution anisotropy on magnetic fabrics in igneous rocks, which has so far only been predicted by experimental and theoretical models. Our results have first-order implications for the interpretation of petrofabrics using magnetic methods.Publisher PDFPeer reviewe

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac