The influence of the concentration of montmorillonite containing sorbent and pH of the culture medium on the antibiotic sensitivity of Escherichia coli, as well as the effect of ground on growth of Escherichia

The aim of this work was the comparative sensitivity test of E. coli to enrofloxacin and doxycycline and bactericidal action of montmorillonite containing sorbent and its combination with enrofloxacin, as well as the influence of the concentration of the enriched montmorillonite containing sorbent and pH of the culture medium on the antibiotic sensitivity of Escherichia coli. The sensitivity test of Escherichia coli to enrofloxacin, and the combination of enrofloxacin with sorbent was performd by the method of double serial dilutions of drugs in a liquid culture mediu

Influence of the enterosorbent on quality of eggs

In this article research the influence of natural montmorillonite containing sorbent used as a mineral supplement in animal feed in an amount of 30 g per 1 kg of sorbent feed on commercial quality table eggs at his dacha hens-layers. Found that feeding hens’ mineral feed additive reduces the toxic chemical elements in a chicken egg. It was found that the weight of the eggs in the experimental group compared to the control group, was significantly increased by 3,5% (p <0,05) after the completion of feeding the mineral sorbent. Despite the fact that in the control group, egg weight did not change and remained virtually unchange

Ubiquinol ameliorates endothelial dysfunction and increases expression of miRNA-34a in a rat model of pulmonary hypertension

In this research, we evaluate the effect of intravenously administrated solubilized ubiquinol on 4-week monocrotalin-induced pulmonary hypertension (PH) in rat

Research into in-service deterioration of ball-rolling rolls

This work features research findings on causes of deterioration occurring in ball-rolling rolls. The causes of such deterioration may originate not only in the excessive loads application and the use of bottleneck operating parameters but also in the specific process being implemented and the equipment used to heat-treat the rolling rolls. The deterioration occurring in roll flanges having a relatively small thickness was used to demonstrate that, to prevent the deterioration of ball-rolling roll flanges, the mill design must accommodate the minimum allowable flange widths obtained as a function of maximum allowable loads and the roll pass calculations. The next example of roll deterioration involved the use of a gas furnace to implement the heating stage of the quench hardening process for rolls made of 35KhGSA steel, which, as a result, developed roll deterioration. The use of rolls of the same steel grade, which were heat-treated in an arc furnace prior to being quenched, has been shown to deliver long-lasting positive results evidenced by many years of their operation. Rolls made of 5KhNM steel have also been shown to respond well to the heat treatment preceding the quenching process, but this time, the treatment was implemented in a gas furnace. © Published under licence by IOP Publishing Ltd

Molecular architecture influences on material properties of pharmaceutical compounds

Salt formation has extensively been studied as a strategy to improve drug solubility but it has not been explored as a strategy to improve mechanical properties. A better understanding of which factors of the solid state can have an influence in the mechanical properties of pharmaceutical powders can help to optimise and reduce cost of tablet manufacturing. The aim of this study was to form different series of amine salts of flurbiprofen, gemfibrozil and diclofenac and to establish predictive relationships between architectural characteristics and physicochemical and mechanical properties of the salts. For this purpose, three different carboxylic acid drugs were selected: flurbiprofen, gemfibrozil and diclofenac, similar in size but varying in flexibility and shape and three different series of counterions were also chosen: one with increasing bulk and no hydroxyl groups to limit the hydrogen bonding potential; a second one with increasing number of hydroxyl groups and finally a third series, related to the latter in number of hydroxyl groups but with different molecular shape and flexibility. Physico-chemical characterization was performed (DSC, TGA, solubility, intrinsic dissolution rate, particle size, true density) and mechanical properties measured using a compaction replicator. Strained molecular conformations produce weaker compacts as they have higher energy than preferred conformations that usually lie close to energy minimums and oppose plastic deformation. It was observed that slip planes, which correspond to regions of weakest interaction between the planes, were associated with improved plasticity and stronger compacts. Apart from hydrogen bonds, profuse van der Waals forces can result in ineffective slip planes. Salts displaying two-dimensional densely hydrogen bonded layers produced stronger compacts than salts showing one-dimensional networks of non-bonded columns, probably by reducing the attachment energy between layers. When hydrogen bonds are created intramolecularly, it is possible that the mechanical properties are compromised as they do not contribute so much to create twodimensional densely bonded layers and they can force molecules into strained conformations. Some types of hydrogen bonding network may be associated with improved mechanical properties, such as type II, or R (10) 3 4 using graph-set notation, versus type III, or R (12) 4 8 , columns. This work clearly demonstrates the potential of investigating crystal structure-mechanical property relationship in pharmaceutical materials.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Фенотипический профиль клеток моноцитарно-макрофагального ряда в зависимости от состояния респираторного эпителия

The mechanism of the relationship between pretumor changes in the bronchial respiratory epithelium and the risk of progression of non-small cell lung cancer (NSCLC) remains unclear. It has been suggested that the relationship between reactive changes in the bronchial mucosa and NSCLC progression may be caused by the functional status of monocytic-macrophage cells as important participants in infammation, which determines both the risk of premalignant changes in the epithelium and malignant progression. The purpose of the study was to investigate the phenotypic profle of peripheral blood monocytes and macrophages induced from monocytes in vitro depending on the state of respiratory epithelium in NSCLC patients. Material and Methods. The study included 39 patients with newly diagnosed NSCLC. Based on the morphological examination of small bronchi taken at the distance of 3–5 cm from the tumor, patients were divided into the following groups depending on the type of pretumor changes: no pretumor changes (n=6), isolated basal cell hyperplasia (BCH) (n=13), combination of BCH and squamous metaplasia (SM) (n=3), combination of unchanged epithelium and focal BCH (n=17). The phenotypic features of peripheral blood monocytes and in vitro-induced macrophages were assessed before treatment using fow cytometry. Results. The state of the respiratory epithelium in NSCLC patients prior to the start of anticancer treatment was associated with the phenotypic features of peripheral blood monocytes, but not with the profle of macrophages induced from them. Distortion of the response of induced macrophages to the polarizing stimuli was observed in NSCLC patients: the cultured cells responded to both M1 and M2 inducers (LPS and IL-4, respectively) with a phenotype shift to M2, while the CD206 marker expression varied depending on the presence and type of pretumor changes. Conclusion. The phenotypic profle of peripheral blood monocytes was associated with the state of the respiratory epithelium in NSCLC patients before anti-tumor treatment, but not with the phenotypic features of induced macrophages.Механизм взаимосвязи предопухолевых изменений в респираторном эпителии бронхов с риском прогрессирования немелкоклеточного рака легкого (НМРЛ) остается неясным. Предполагается, что связь между реактивными изменениями в слизистой бронхов и прогрессированием НМРЛ может быть обусловлена функциональным статусом клеток моноцитарно-макрофагальной линии, являющихся важными участниками процессов воспаления, которое определяет как риск предопухолевых изменений в эпителии, так и прогрессирование злокачественного новообразования. Цель исследования – изучение фенотипического профиля моноцитов периферической крови и макрофагов, индуцированных из моноцитов in vitro, в зависимости от состояния респираторного эпителия у больных НМРЛ. Материал и методы. В исследование включено 39 больных с впервые диагностированным НМРЛ. На основании результатов морфологического исследования мелких бронхов, взятых на расстоянии 3–5 см от опухоли, в зависимости от варианта предопухолевых изменений больные были разделены на следующие группы: отсутствие предопухолевых изменений (n=6), изолированная базальноклеточная гиперплазия (БКГ) (n=13), сочетание БКГ и плоскоклеточной метаплазии (ПМ) (n=3), сочетание неизмененного эпителия и очаговой БКГ (n=17). Оценка фенотипических особенностей моноцитов периферической крови и индуцированных in vitro макрофагов проводилась до начала противоопухолевого лечения больных методом проточной цитометрии. Результаты. Состояние респираторного эпителия у больных НМРЛ до начала противоопухолевого лечения ассоциировано с фенотипическими особенностями моноцитов периферической крови, но не с профилем индуцированных из них макрофагов. У больных НМРЛ отмечено искажение ответа индуцированных макрофагов на поляризующие стимулы: как на М1-, так и на М2-индукторы (LPS и IL-4, соответственно) культивируемые клетки отвечали сдвигом фенотипа в М2-сторону, при этом экспрессия маркера CD206 варьировала в некотором диапазоне в зависимости от наличия и варианта предопухолевых изменений. Заключение. Фенотипический профиль моноцитов периферической крови ассоциирован с состоянием респираторного эпителия у больных НМРЛ до начала противоопухолевого лечения, но не с фенотипическими особенностями индуцированных макрофагов.

Lowering IceCube’s energy threshold for point source searches in the southern sky

Observation of a point source of astrophysical neutrinos would be a "smoking gun" signature of a cosmic-ray accelerator. While IceCube has recently discovered a diffuse flux of astrophysical neutrinos, no localized point source has been observed. Previous IceCube searches for point sources in the southern sky were restricted by either an energy threshold above a few hundred TeV or poor neutrino angular resolution. Here we present a search for southern sky point sources with greatly improved sensitivities to neutrinos with energies below 100 TeV. By selecting charged-current nu(mu) interacting inside the detector, we reduce the atmospheric background while retaining efficiency for astrophysical neutrino-induced events reconstructed with sub-degree angular resolution. The new event sample covers three years of detector data and leads to a factor of 10 improvement in sensitivity to point sources emitting below 100 TeV in the southern sky. No statistically significant evidence of point sources was found, and upper limits are set on neutrino emission from individual sources. A posteriori analysis of the highest-energy (similar to 100 TeV) starting event in the sample found that this event alone represents a 2.8 sigma deviation from the hypothesis that the data consists only of atmospheric background

Improved limits on dark matter annihilation in the Sun with the 79-string IceCube detector and implications for supersymmetry

We present an improved event-level likelihood formalism for including neutrino telescope data in global fits to new physics. We derive limits on spin-dependent dark matter-proton scattering by employing the new formalism in a re-analysis of data from the 79-string IceCube search for dark matter annihilation in the Sun, including explicit energy information for each event. The new analysis excludes a number of models in the weak-scale minimal supersymmetric standard model (MSSM) for the first time. This work is accompanied by the public release of the 79-string IceCube data, as well as an associated computer code for applying the new likelihood to arbitrary dark matter models.Comment: 24 pages, 8 figs, 1 table. Contact authors: Pat Scott & Matthias Danninger. Likelihood tool available at http://nulike.hepforge.org. v2: small updates to address JCAP referee repor

Search for transient optical counterparts to high-energy IceCube neutrinos with Pan-STARRS1

In order to identify the sources of the observed diffuse high-energy neutrino flux, it is crucial to discover their electromagnetic counterparts. IceCube began releasing alerts for single high-energy ($E > 60$ TeV) neutrino detections with sky localisation regions of order 1 deg radius in 2016. We used Pan-STARRS1 to follow-up five of these alerts during 2016-2017 to search for any optical transients that may be related to the neutrinos. Typically 10-20 faint ($m < 22.5$ mag) extragalactic transients are found within the Pan-STARRS1 footprints and are generally consistent with being unrelated field supernovae (SNe) and AGN. We looked for unusual properties of the detected transients, such as temporal coincidence of explosion epoch with the IceCube timestamp. We found only one transient that had properties worthy of a specific follow-up. In the Pan-STARRS1 imaging for IceCube-160427A (probability to be of astrophysical origin of $\sim$50 %), we found a SN PS16cgx, located at 10.0' from the nominal IceCube direction. Spectroscopic observations of PS16cgx showed that it was an H-poor SN at z = 0.2895. The spectra and light curve resemble some high-energy Type Ic SNe, raising the possibility of a jet driven SN with an explosion epoch temporally coincident with the neutrino detection. However, distinguishing Type Ia and Type Ic SNe at this redshift is notoriously difficult. Based on all available data we conclude that the transient is more likely to be a Type Ia with relatively weak SiII absorption and a fairly normal rest-frame r-band light curve. If, as predicted, there is no high-energy neutrino emission from Type Ia SNe, then PS16cgx must be a random coincidence, and unrelated to the IceCube-160427A. We find no other plausible optical transient for any of the five IceCube events observed down to a 5$\sigma$ limiting magnitude of $m \sim 22$ mag, between 1 day and 25 days after detection.Comment: 20 pages, 6 figures, accepted to A&

An All-Sky Search for Three Flavors of Neutrinos from Gamma-Ray Bursts with the IceCube Neutrino Observatory

We present the results and methodology of a search for neutrinos produced in the decay of charged pions created in interactions between protons and gamma-rays during the prompt emission of 807 gamma-ray bursts (GRBs) over the entire sky. This three-year search is the first in IceCube for shower-like Cherenkov light patterns from electron, muon, and tau neutrinos correlated with GRBs. We detect five low-significance events correlated with five GRBs. These events are consistent with the background expectation from atmospheric muons and neutrinos. The results of this search in combination with those of IceCube's four years of searches for track-like Cherenkov light patterns from muon neutrinos correlated with Northern-Hemisphere GRBs produce limits that tightly constrain current models of neutrino and ultra high energy cosmic ray production in GRB fireballs.Comment: 33 pages, 14 figures; minor changes made to match published version in the Astrophysical Journal, 2016 June 2