1,736 research outputs found
An Upstream Hfq Binding Site in the fhlA mRNA Leader Region Facilitates the OxyS-fhlA Interaction
To survive, bacteria must be able to adapt to environmental stresses. Small regulatory RNAs have been implicated as intermediates in a variety of stress-response pathways allowing dynamic gene regulation. The RNA binding protein Hfq facilitates this process in many cases, helping sRNAs base pair with their target mRNAs and initiate gene regulation. Although Hfq has been identified as a critical component in many RNPs, the manner by which Hfq controls these interactions is not known.To test the requirement of Hfq in these mRNA-sRNA complexes, the OxyS-fhlA system was used as a model. OxyS is induced in response to oxidative stress and down regulates the translation of fhlA, a gene encoding a transcriptional activator for formate metabolism. Biophysical characterization of this system previously used a minimal construct of the fhlA mRNA which inadvertently removed a critical element within the leader sequence of this mRNA that effected thermodynamics and kinetics for the interaction with Hfq.Herein, we report thermodynamic, kinetic and structural mapping studies during binary and ternary complex formation between Hfq, OxyS and fhlA mRNA. Hfq binds fhlA mRNA using both the proximal and distal surfaces and stimulates association kinetics between the sRNA and mRNA but remains bound to fhlA forming a ternary complex. The upstream Hfq binding element within fhlA is similar to (ARN)(x) elements recently identified in other mRNAs regulated by Hfq. This work leads to a kinetic model for the dynamics of these complexes and the regulation of gene expression by bacterial sRNAs
Deep UV Luminosity Functions at the Infall Region of the Coma Cluster
We have used deep GALEX observations at the infall region of the Coma cluster
to measure the faintest UV luminosity functions (LFs) presented for a rich
galaxy cluster thus far. The Coma UV LFs are measured to M_UV = -10.5 in the
GALEX FUV and NUV bands, or 3.5 mag fainter than previous studies, and reach
the dwarf early-type galaxy population in Coma for the first time. The
Schechter faint-end slopes (alpha = -1.39 in both GALEX bands) are shallower
than reported in previous Coma UV LF studies owing to a flatter LF at faint
magnitudes. A Gaussian-plus-Schechter model provides a slightly better
parametrization of the UV LFs resulting in a faint-end slope of ~ -1.15 in both
GALEX bands. The two-component model gives faint-end slopes shallower than -1
(a turnover) for the LFs constructed separately for passive and star forming
galaxies. The UV LFs for star forming galaxies show a turnover at M_UV ~ -14
owing to a deficit of dwarf star forming galaxies in Coma with stellar masses
below M*=10^8 Msun. A similar turnover is identified in recent UV LFs measured
for the Virgo cluster suggesting this may be a common feature of local galaxy
clusters, whereas the field UV LFs continue to rise at faint magnitudes. We did
not identify an excess of passive galaxies as would be expected if the missing
dwarf star forming galaxies were quenched inside the cluster. In fact, the LFs
for both dwarf passive and star forming galaxies show the same turnover at
faint magnitudes. We discuss the possible origin of the missing dwarf star
forming galaxies in Coma and their expected properties based on comparisons to
local field galaxies.Comment: accepted for publication in Ap
Horizontal axis wind turbine performance analysis
The present work uses the method of Blade Element Momentum Theory as suggested by Hansen. The
method applied to three blade models adopted from Rahgozar S. with the airfoil data used the data provided by Wood
D. The wind turbine performance described in term of the thrust coefficient CT, torque coefficient CQ and the power
coefficient Cp . These three coefficient can be deduced from the Momentum theory or from the Blade element
Theory(BET). The present work found the performance coefficient derived from the Momentum theory tent to over
estimate. It is suggested to used the BET formulation in presenting these three coefficients. In overall the Blade
Element Momentum Theory follows the step by step as described by Hansen work well for these three blade models.
However a little adjustment on the blade data is needed. To the case of two bladed horizontal axis wind turbine,
Hansen’s approach works well over if the blade radius is RB the calculation should start from r = 0.1RB
Coral Reef Spatial Distribution in Wangi-wangi Island Waters, Wakatobi
Coral reefs contribute significant benefits in coastal area in Wangi-wangi Island, Wakatobi in terms of their ecological functions to marine biota and socio-economical services to local coastal communities. Therefore, it is importance to observe coral reef condition and its spatial distribution around Wangi-wangi island waters, Wakatobi. In this study Point Intercept Transect (PIT) and GIS tools were used to observe and analyze coral reef condition in Wangi-wangi island waters, Wakatobi. The results showed that coral reef condition in Wangi-wangi island waters can be categorized into moderate and good conditions with coverage percentage ranging from 28 – 60%. Based on spatial analysis non-acropora coral was found in greater cover percentages in Waha village, Sombu village, and Kapota island than other locations. Soft coral cover percentage was also found in larger cover percentage in Waha and Sombu regions than other locations
String Propagation in Bianchi Type I models: Dynamical anisotropy Damping and Consequences
A generic ansatz is introduced which provides families of exact solutions to
the equations of motion and constraints for null-strings in Bianchi type I
cosmological models. This is achieved irrespective of the form of the metric.
Within classes of dilaton cosmologies a backreaction mapping relation is
established where the null string leads to more or less anisotropic members of
the family. The equations of motion and constraints for the generic model are
casted in their first order form and integrated both analytically and
numerically.Comment: 37 pages, 11 figure
Host galaxy colour gradients and accretion disc obscuration in AEGIS z~1 X-ray-selected active galactic nuclei
We describe the effect of AGN light on host galaxy optical and UV-optical
colours, as determined from X-ray-selected AGN host galaxies at z~1, and
compare the AGN host galaxy colours to those of a control sample matched to the
AGN sample in both redshift and stellar mass. We identify as X-ray-selected
AGNs 8.7 +4/-3 per cent of the red-sequence control galaxies, 9.8 +/-3 per cent
of the blue-cloud control galaxies, and 14.7 +4/-3 per cent of the green-valley
control galaxies. The nuclear colours of AGN hosts are generally bluer than
their outer colours, while the control galaxies exhibit redder nuclei. AGNs in
blue-cloud host galaxies experience less X-ray obscuration, while AGNs in
red-sequence hosts have more, which is the reverse of what is expected from
general considerations of the interstellar medium. Outer and integrated colours
of AGN hosts generally agree with the control galaxies, regardless of X-ray
obscuration, but the nuclear colours of unobscured AGNs are typically much
bluer, especially for X-ray luminous objects. Visible point sources are seen in
many of these, indicating that the nuclear colours have been contaminated by
AGN light and that obscuration of the X-ray radiation and visible light are
therefore highly correlated. Red AGN hosts are typically slightly bluer than
red-sequence control galaxies, which suggests that their stellar populations
are slightly younger. We compare these colour data to current models of AGN
formation. The unexpected trend of less X-ray obscuration in blue-cloud
galaxies and more in red-sequence galaxies is problematic for all AGN feedback
models, in which gas and dust is thought to be removed as star formation shuts
down. [See paper for full abstract.]Comment: Accepted to MNRAS. 19 pages, 14 figures, 1 table; table, four figures
(4, 6, 11, 13) revised to reflect corrected values for one of our objects;
results unchange
Large-scale magnetic fields from inflation due to Chern-Simons-like effective interaction
We discuss the generation of large-scale magnetic fields due to the breaking
of the conformal invariance in the electromagnetic field through the -even
dimension-six Chern-Simons-like effective interaction with a fermion current in
inflationary cosmology. It is shown that the magnetic fields on 1Mpc scale with
the field strength of G at the present time can be generated even
for the scale of the effective interaction being the Planck scale.Comment: 13 pages, 2 figures, additional explanations include
Mid-IR Luminosities and UV/Optical Star Formation Rates at z<1.4
UV continuum and mid-IR emission constitute two widely used star formation
indicators at intermediate and high redshifts. We study 2430 galaxies with
z<1.4 in the Extended Groth Strip with MIPS 24 mic observations from FIDEL,
spectroscopy from DEEP2, and UV, optical, and near-IR photometry from AEGIS.
The data are coupled with stellar population models and Bayesian SED fitting to
estimate dust-corrected SFRs. In order to probe the dust heating from stellar
populations of various ages, the derived SFRs were averaged over various
timescales--from 100 Myr for "current" SFR to 1--3 Gyr for long-timescale SFRs.
These SED-based UV/optical SFRs are compared to total infrared luminosities
extrapolated from 24 mic observations. We find that for the blue, actively star
forming galaxies the correlation between the IR luminosity and the UV/optical
SFR shows a decrease in scatter when going from shorter to longer SFR-averaging
timescales. We interpret this as the greater role of intermediate age stellar
populations in heating the dust than what is typically assumed. This holds over
the entire redshift range. Many so-called green valley galaxies are simply
dust-obscured actively star-forming galaxies. However, there exist 24
mic-detected galaxies, some with L>10^11 L_sun, yet with little current star
formation. For them a reasonable amount of dust absorption of stellar light is
sufficient to produce the observed levels of IR. In our sample optical and
X-ray AGNs do not contribute on average more than ~50% to the mid-IR
luminosity, and we see no evidence for a large population of "IR excess"
galaxies (Abridged).Comment: Accepted for publication in ApJ. Content identical to arXiv version
1. No color figure
Fabrication of smart glass electrochromic device using rf magnetron sputtering
Electrochromic device is an important functional device to control the amount of light
through a glass. It usually used in sunlight control window glazing for buildings and
automobile. The important feature of electrochromic glass is the ability to response
toward the apply voltage in shortest time, and endurance to maintain in color shape after
apply voltage. In this thesis, the oxygen gas percentage is optimized during the
fabrication of tungsten trioxide (WO3) as an electrochromic glass for window glazing
application by using RF magnetron sputtering. The oxygen flow rate for the deposition
is varied from 10sccm -22sccm which is 25%, 27%, 30%, and 35% of oxygen flow. The
structures of WO3 were investigated using X-Ray diffraction, Field effect scanning
electron microscopy (Fe-Sem) and Atomic force microscopy (AFM). The
electrochromic properties were characterized by a cyclic voltammogram and UV-Vis
absorption spectra. The results show that nanocrystalline film with particle size of
51.54nm was deposited at 27% oxygen flow rate has the largest charge capacity and
coloration efficiency among the others. The time respond taken for complete coloration
at 4V is 2second. This result is a starting point for future work such as optimizing the
film thickness or doping by other metals
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