3D surface topography and reflectivity of anisotropic etched silicon micromirrors for BioMEMS

This paper examines wet and dry fabrication of vertical micro-mirrors in (110) silicon for use in an innovative BioMEMS integrating gripping and micro force sensing functionalities. Wet anisotropic chemical etching in potassium hydroxide (KOH) and tetramethyl ammonium hydroxide (TMAH) with and without isopropanol alcohol (IPA) additive was examined. Deep Reactive Ion Etched samples were produced using inductive coupled process. 3D surface roughness of samples was examined using scanning electron microscope, interferometric profilometer and atomic force microscopy. An optic fiber displacement sensor was exploited to measure the reflectivity of uncoated or coated samples with evaporated metallic thin film. The research aimed to find optimal fabrication technique for fabricating vertical micro-mirrors in polymer based BioMEMS. TMAH etched silicon samples with surface roughness R a = 15.1 nm showed highest reflectivity of all structures fabricated, reflectivity was more than doubled by adding a 10 nm layer of evaporated aluminum coating

Collagen-hyaluronic acid scaffolds for adipose tissue engineering.

Three-dimensional (3-D) in vitro models of the mammary gland require a scaffold matrix that supports the development of adipose stroma within a robust freely permeable matrix. 3-D porous collagen-hyaluronic acid (HA: 7.5% and 15%) scaffolds were produced by controlled freeze-drying technique and crosslinking with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride. All scaffolds displayed uniform, interconnected pore structure (total porosity approximately 85%). Physical and chemical analysis showed no signs of collagen denaturation during the formation process. The values of thermal characteristics indicated that crosslinking occurred and that its efficiency was enhanced by the presence of HA. Although the crosslinking reduced the swelling of the strut material in water, the collagen-HA matrix as a whole tended to swell more and show higher dissolution resistance than pure collagen samples. The compressive modulus and elastic collapse stress were higher for collagen-HA composites. All the scaffolds were shown to support the proliferation and differentiation 3T3-L1 preadipocytes while collagen-HA samples maintained a significantly increased proportion of cycling cells (Ki-67+). Furthermore, collagen-HA composites displayed significantly raised Adipsin gene expression with adipogenic culture supplementation for 8 days vs. control conditions. These results indicate that collagen-HA scaffolds may offer robust, freely permeable 3-D matrices that enhance mammary stromal tissue development in vitro.This was supported by the Biotechnology and Biological Sciences Research Council

A consistent model for leptogenesis, dark matter and the IceCube signal

We discuss a left-right symmetric extension of the Standard Model in which the three additional right-handed neutrinos play a central role in explaining the baryon asymmetry of the Universe, the dark matter abundance and the ultra energetic signal detected by the IceCube experiment. The energy spectrum and neutrino flux measured by IceCube are ascribed to the decays of the lightest right-handed neutrino $N_1$, thus fixing its mass and lifetime, while the production of $N_1$ in the primordial thermal bath occurs via a freeze-in mechanism driven by the additional $SU(2)_R$ interactions. The constraints imposed by IceCube and the dark matter abundance allow nonetheless the heavier right-handed neutrinos to realize a standard type-I seesaw leptogenesis, with the $B-L$ asymmetry dominantly produced by the next-to-lightest neutrino $N_2$. Further consequences and predictions of the model are that: the $N_1$ production implies a specific power-law relation between the reheating temperature of the Universe and the vacuum expectation value of the $SU(2)_R$ triplet; leptogenesis imposes a lower bound on the reheating temperature of the Universe at 7\times10^9\,\mbox{GeV}. Additionally, the model requires a vanishing absolute neutrino mass scale $m_1\simeq0$.Comment: 19 pages, 4 figures. Constraints from cosmic-ray antiprotons and gamma rays added, with hadrophobic assignment of the matter multiplets to satisfy bounds. References added. Matches version published in JHE

Enumerating Cyclic Orientations of a Graph

Acyclic and cyclic orientations of an undirected graph have been widely studied for their importance: an orientation is acyclic if it assigns a direction to each edge so as to obtain a directed acyclic graph (DAG) with the same vertex set; it is cyclic otherwise. As far as we know, only the enumeration of acyclic orientations has been addressed in the literature. In this paper, we pose the problem of efficiently enumerating all the \emph{cyclic} orientations of an undirected connected graph with $n$ vertices and $m$ edges, observing that it cannot be solved using algorithmic techniques previously employed for enumerating acyclic orientations.We show that the problem is of independent interest from both combinatorial and algorithmic points of view, and that each cyclic orientation can be listed with $\tilde{O}(m)$ delay time. Space usage is $O(m)$ with an additional setup cost of $O(n^2)$ time before the enumeration begins, or $O(mn)$ with a setup cost of $\tilde{O}(m)$ time

The a-number of hyperelliptic curves

It is known that for a smooth hyperelliptic curve to have a large $a$-number, the genus must be small relative to the characteristic of the field, $p>0$, over which the curve is defined. It was proven by Elkin that for a genus $g$ hyperelliptic curve $C$ to have $a_C=g-1$, the genus is bounded by $g<\frac{3p}{2}$. In this paper, we show that this bound can be lowered to $g <p$. The method of proof is to force the Cartier-Manin matrix to have rank one and examine what restrictions that places on the affine equation defining the hyperelliptic curve. We then use this bound to summarize what is known about the existence of such curves when $p=3,5$ and $7$.Comment: 7 pages. v2: revised and improved the proof of the main theorem based on suggestions from the referee. To appear in the proceedings volume of Women in Numbers Europe-

Classical orbital paramagnetism in non-equilibrium steady state

We report the results of our numerical simulation of classical-dissipative dynamics of a charged particle subjected to a non-markovian stochastic forcing. We find that the system develops a steady-state orbital magnetic moment in the presence of a static magnetic field. Very significantly, the sign of the orbital magnetic moment turns out to be {\it paramagnetic} for our choice of parameters, varied over a wide range. This is shown specifically for the case of classical dynamics driven by a Kubo-Anderson type non-markovian noise. Natural spatial boundary condition was imposed through (1) a soft (harmonic) confining potential, and (2) a hard potential, approximating a reflecting wall. There was no noticeable qualitative difference. What appears to be crucial to the orbital magnetic effect noticed here is the non-markovian property of the driving noise chosen. Experimental realization of this effect on the laboratory scale, and its possible implications are briefly discussed. We would like to emphasize that the above steady-state classical orbital paramagnetic moment complements, rather than contradicts the Bohr-van Leeuwen (BvL) theorem on the absence of classical orbital diamagnetism in thermodynamic equilibrium.Comment: 6 pages, 4 figures, Has appeared in Journal of Astrophysics and Astronomy special issue on 'Physics of Neutron Stars and Related Objects', celebrating the 75th birth-year of G. Srinivasa

Tailoring Gold Nanoparticle Characteristics and the Impact on Aqueous-Phase Oxidation of Glycerol

Poly(vinyl alcohol) (PVA)-stabilized Au nanoparticles (NPs) were synthesized by colloidal methods in which temperature variations (−75 to 75 °C) and mixed H2O/EtOH solvent ratios (0, 50, and 100 vol/vol) were used. The resulting Au NPs were immobilized on TiO2 (P25), and their catalytic performance was investigated for the liquid phase oxidation of glycerol. For each unique solvent system, there was a systematic increase in the average Au particle diameter as the temperature of the colloidal preparation increased. Generation of the Au NPs in H2O at 1 °C resulted in a high observed activity compared with current Au/TiO2 catalysts (turnover frequency = 915 h–1). Interestingly, Au catalysts with similar average particle sizes but prepared under different conditions had contrasting catalytic performance. For the most active catalyst, aberration-corrected high angle annular dark field scanning transmission electron microscopy analysis identified the presence of isolated Au clusters (from 1 to 5 atoms) for the first time using a modified colloidal method, which was supported by experimental and computational CO adsorption studies. It is proposed that the variations in the populations of these species, in combination with other solvent/PVA effects, is responsible for the contrasting catalytic properties

The sexually dimorphic behaviour of adult Drosophila suzukii: elevated female locomotor activity and loss of siesta is a post-mating response

The polyphagous Drosophila suzukii is a highly invasive species that causes extensive damage to a wide range of berry and stone fruit crops. A better understanding of its biology and especially its behaviour will aid the development of new control strategies. We investigated the locomotor behaviour of D. suzukii in a semi-natural environment resembling a typical summer in northern England and show that adult female D. suzukii are at least 4-fold more active during daylight hours than adult males. This result was reproduced in several laboratory environments and was shown to be a robust feature of mated, but not virgin, female flies. Both males and virgin females kept on a 12 h light:12 h dark (12LD) cycle and constant temperature displayed night-time inactivity (sleep) followed by weak activity in the morning, an afternoon period of quiescence (siesta) and then a prominent evening peak of activity. Both the siesta and the sharp evening peak at lights off were severely reduced in females after mating. Flies of either sex entrained in 12LD displayed a circadian pattern of activity in constant darkness confirming the importance of an endogenous clock in regulating adult activity. This response of females to mating is similar to that elicited in female Drosophila melanogaster by the male sex peptide (SP). We used mass spectrometry to identify a molecular ion (m/z, 5145) corresponding to the poly-hydroxylated SP of D. suzukii and to show that this molecule is transferred to the female reproductive tract during copulation. We propose that the siesta experienced by male and virgin female D. suzukii is an adaptation to avoid unnecessary exposure to the afternoon sun, but that mated females faced with the challenge of obtaining resources for egg production and finding oviposition sites take greater risks, and we suggest that the change in female behaviour is induced by the male SP

Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties.

Chitosan/collagen (Chit/Col) blends have demonstrated great potential for use in tissue engineering (TE) applications. However, there exists a lack of detailed study on the influence of important design parameters (i.e, component ratio or crosslinking methods) on the essential properties of the scaffolds (morphology, mechanical stiffness, swelling, degradation and cytotoxicity). This work entailed a systematic study of these essential properties of three Chit/Col compositions, covering a wide range of component ratios and using different crosslinking methods. Our results showed the possibility of tailoring these properties by changing component ratios, since different interactions occurred between Chit/Col: samples with Chit-enriched compositions showed a hydrogen-bonding type complex (HC), whereas a self-crosslinking phenomenon was induced in Col-enriched scaffolds. Additionally, material and biological properties of the resultant matrices were further adjusted and tuned by changing crosslinking conditions. In such way, we obtained a wide range of scaffolds whose properties were tailored to meet specific needs of TE applications.The authors are grateful to Dr. von Kobbe (Chimera Pharma of Bionostra Group) for the gift of MCF7 cells. The financial support of the Ministerio de Ciencia e Innovación of Spain (FIS PS09/01513), and the FPI grant from UCM to A. Martínez are gratefully acknowledged.This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.carbpol.2015.06.08

Photonic polarization gears for ultra-sensitive angular measurements

Quantum metrology bears a great promise in enhancing measurement precision, but is unlikely to become practical in the near future. Its concepts can nevertheless inspire classical or hybrid methods of immediate value. Here, we demonstrate NOON-like photonic states of m quanta of angular momentum up to m=100, in a setup that acts as a "photonic gear", converting, for each photon, a mechanical rotation of an angle {\theta} into an amplified rotation of the optical polarization by m{\theta}, corresponding to a "super-resolving" Malus' law. We show that this effect leads to single-photon angular measurements with the same precision of polarization-only quantum strategies with m photons, but robust to photon losses. Moreover, we combine the gear effect with the quantum enhancement due to entanglement, thus exploiting the advantages of both approaches. The high "gear ratio" m boosts the current state-of-the-art of optical non-contact angular measurements by almost two orders of magnitude.Comment: 10 pages, 4 figures, + supplementary information (10 pages, 3 figures