Identification moléculaire des souches de mycobactéries

L’identification moléculaire des souches de mycobactéries disponibles dans notre laboratoire a été réalisée. L’amplification par PCR des gènes de hsp, ARNr16S, espaceurs intergéniques ARNr16S-23S suivie de l’électrophorèse sur gel d’agarose des fragments obtenus avec les oligonucléotides Tb11 et Tb12, 248 et 42, Int16S et Int23S, révèle la constance dans la taille des fragments pour toutes les souches et par paire d’oligonucléotides. Ces résultats sont confirmés par la RFLP qui ne montre pas de différences significatives entre les différentes souches. Dans ce cas la discrimination des souches est difficile, on peut penser qu’il s’agit d’un seul genre. Par contre la taille des fragments obtenus avec les oligonucléotides H49 et H50, GyrAF et GyrAR permet de distinguer trois groupes de souches, les souches 6PY, C-8, C-18, et C-19 forment un premier groupe, les souches BHF004, C-20 et SPYR forment un deuxième groupe, et enfin la souche PYR-1 forme un troisième groupe.Le séquençage et l’alignement multiple avec Clustal des séquences en comparaison d’une part avec Mycobacterium gilvum pour le premier groupe et d’autre part avec Mycobacterium vanbaalenii et Mycobacterium austroafricanum pour le deuxième groupe, confirment par le taux de similarité élevé (99- 100%) cette classification. Un arbre phylogénétique basée sur les séquences partielles du gène hsp65, permet de situer les nouvelles par rapport aux autres mycobactéries .Cela corrobore bien avec nos résultats, tout en confirmant la cohérence de ces trois espèces dans le genre monophylétique Mycobacterium.Mots-clés : mycobactéries, oligonucléotides, amplification par PCR, séquençage, alignement multiple

A graphene electron lens

International audienceAn epitaxial layer of graphene was grown on a pre patterned 6H-SiC(0001) crystal. The graphene smoothly covers the hexagonal nano-holes in the substrate without the introduction of small angle grain boundaries or dislocations. This is achieved by an elastic deformation of the graphene by ~0.3% in accordance to its large elastic strain limit. This elastic stretching of the graphene leads to a modification of the band structure and to a local lowering of the electron group velocity of the graphene. We propose to use this effect to focus two-dimensional electrons in analogy to simple optical lenses

School Delay of Child in Brazzaville (Congo)

Abstract The aim of this study was to determine the prevalence of school delay in school age children and to identify its determinants in Brazzaville. A case-control study, comparing school age children with a school delay (Group 1 or cases) to those who had not school delay (Group 2 or control), was carried out between February and July 2013. It included students of CM2 (primary 6) and those of 3 ème (form 4) of both public and private schools of Brazzaville. The sample selection was made according to a random survey by strata, the number of strata was set to 2. The sample consisted of 2064 pupils including 1138 girls (55.1%). A total of 2064 students (1138 male/926 female), 792 of them had a school delay, with a prevalence of 38.3%. It was 27% in the private sector and 46.9% in the public one; 28.2% in primary education compared to 48.1% in the college; 27.8% in girls and 48.8% in boys (p < 0.001). 21.4% of students in CM2 (primary 6) of the public and 16.7% in the private sector had repeated classes 3 times; 4.2% of students in 3ème (form 4) of the public education had repeated classes 4 times. Kindergarten program attendance had a positive effect on later school performances (p < 0.05). The parent's level of education and socio-economic status of the family (low and mean for students in CM2 of public schools and of 3ème of private schools, high for pupils in CM2 of private schools) and underweight among pupils in CM2 of the private sector were significantly correlated with school delay (p < 0.05). The prevalence of school delay was high in Brazzaville (38.3%), boys were more affected than girls. The kindergarten program attendance was found to have a positive effect on later school performances, while parent's low level of education and low socioeconomic status of the family significantly influenced the rate of school delay in children in Brazzaville. The high prevalence of school delay in child in Brazzaville imposed substantial actions, in addition to the efforts already made. Keywords School Delay, Child, Brazzaville J. R. Mabiala-Babela et al. 42

Regional Disparities and Investment-Cash Flow Sensitivity: Evidence from Chinese Listed Firms

In China, regional disparities are important. We examine the difference in the sensitivity of investment to cash flow between firms in inland regions and those in coastal regions. By using the financial data of Chinese listed firms, we found that firms in inland regions rely more on their internal funds in terms of their investment activities than those in coastal regions and that the sensitivity gap between inland and coastal firms widened in the recent contractionary monetary policy period. This suggests that firms in inland regions are harder to obtain outside funds due to unfavorable social and economic environments for inland firms. Our findings suggest that capital markets in China respond rationally to the potential impact of regional disparities on a firm’s performance

A large-scale study of a poultry trading network in Bangladesh: implications for control and surveillance of avian influenza viruses

Since its first report in 2007, avian influenza (AI) has been endemic in Bangladesh. While live poultry marketing is widespread throughout the country and known to influence AI dissemination and persistence, trading patterns have not been described. The aim of this study is to assess poultry trading practices and features of the poultry trading networks which could promote AI spread, and their potential implications for disease control and surveillance. Data on poultry trading practices was collected from 849 poultry traders during a cross-sectional survey in 138 live bird markets (LBMs) across 17 different districts of Bangladesh. The quantity and origins of traded poultry were assessed for each poultry type in surveyed LBMs. The network of contacts between farms and LBMs resulting from commercial movements of live poultry was constructed to assess its connectivity and to identify the key premises influencing it

Obesity, but not hypohydration, mediates changes in mental task load during passive heating in females

Background The independent effects of hypohydration and hyperthermia on cognition and mood is unclear since the two stresses often confound each other. Further, it is unknown if obese individuals have the same impairments during hyperthermia and hypohydration that is often observed in non-obese individuals. Methods The current study was designed to assess the independent and combined effects of mild hypohydration and hyperthermia on cognition, mood, and mental task load in obese and non-obese females. Twenty-one healthy females participated in two passive heating trials, wherein they were either euhydrated or hypohydrated prior to and throughout passive heating. Cognition (ImPACT), mental task load (NASA-TLX), and mood (Brunel Mood Scale; BRUMS) were measured before and after a 1.0 °C increase in core temperature (TC). Results After a 1.0 °C TC elevation, hypohydration resulted in greater (p  0.05). Hyperthermia, regardless of hydration status, impaired (∼5 A.U) measures of memory-based cognition (verbal and visual memory), and increased mental task load, while worsening mood (p  0.05). Conclusion These data indicate that hyperthermia independently impairs memory-based aspects of cognitive performance, mental task load, and leads to a negative mood state. Mild hypohydration did not exacerbate the effects of hyperthermia. However, obese individuals had increased mental task load during hyperthermia

Felsic crust development in the Kaapvaal Craton, South Africa: A reference sample collection to investigate a billion years of geological history

The crust of the Kaapvaal craton accreted throughout the Archaean over nearly 1 billion years. It provides a unique example of the various geological processes that shape Earth's continental crust, and is illustrated by a reference collection of granitoids and mafic rocks (SWASA collection). This sample collection is fully characterised in term of age, major and trace elements, and documents the following multistage history of the craton. In the Barberton area, the initial stages of accretion (stage B·I, > 3.33 Ga and B.II, 3.28—3.21 Ga) correspond to the formation of a sodic (TTG) crust extracted from a near-chondritic reservoir. Stage B.III (ca. 3.1 Ga) corresponds to reworking of this crust, either through intracrustal melting, or via recycling of some material into the mantle and melting of this enriched mantle. Stage B.IV (2.85—2.7 Ga) corresponds to the emplacement of small, discrete plutons involving limited intracrustal reworking. The Northern Kaapvaal craton corresponds to a mobile belt flanking the Barberton cratonic core to the North. Stage NK·I (> 3.1 Ga) resembles stages B·I and B.II: formation of a TTG crust from a chondritic reservoir. In contrast, stage NK.II. (2.97–2.88 Ga) witnesses probable rifting of a cratonic fragment and formation of greenstone basins as well as a new generation of TTGs with both the mafic and felsic magmatism extracted from an isotopically depleted mantle (super-chondritic) reservoir. Intra-crustal reworking dominates stage NK.III (2.88–2.71 Ga), whereas sanukitoids and related granites, involving a mantle contaminated by recycled crustal material, are common during stage NK.IV (ca. 2.67 Ga)

Evaluation of drug administration errors in a teaching hospital

<p>Abstract</p> <p>Background</p> <p>Medication errors can occur at any of the three steps of the medication use process: prescribing, dispensing and administration. We aimed to determine the incidence, type and clinical importance of drug administration errors and to identify risk factors.</p> <p>Methods</p> <p>Prospective study based on disguised observation technique in four wards in a teaching hospital in Paris, France (800 beds). A pharmacist accompanied nurses and witnessed the preparation and administration of drugs to all patients during the three drug rounds on each of six days per ward. Main outcomes were number, type and clinical importance of errors and associated risk factors. Drug administration error rate was calculated with and without wrong time errors. Relationship between the occurrence of errors and potential risk factors were investigated using logistic regression models with random effects.</p> <p>Results</p> <p>Twenty-eight nurses caring for 108 patients were observed. Among 1501 opportunities for error, 415 administrations (430 errors) with one or more errors were detected (27.6%). There were 312 wrong time errors, ten simultaneously with another type of error, resulting in an error rate without wrong time error of 7.5% (113/1501). The most frequently administered drugs were the cardiovascular drugs (425/1501, 28.3%). The highest risks of error in a drug administration were for dermatological drugs. No potentially life-threatening errors were witnessed and 6% of errors were classified as having a serious or significant impact on patients (mainly omission). In multivariate analysis, the occurrence of errors was associated with drug administration route, drug classification (ATC) and the number of patient under the nurse's care.</p> <p>Conclusion</p> <p>Medication administration errors are frequent. The identification of its determinants helps to undertake designed interventions.</p

Subduction or sagduction? Ambiguity in constraining the origin of ultramafic–mafic bodies in the Archean crust of NW Scotland

The Lewisian Complex of NW Scotland is a fragment of the North Atlantic Craton. It comprises mostly Archean tonalite–trondhjemite–granodiorite (TTG) orthogneisses that were variably metamorphosed and reworked in the late Neoarchean to Paleoproterozoic. Within the granulite facies central region of the mainland Lewisian Complex, discontinuous belts composed of ultramafic–mafic rocks and structurally overlying garnet–biotite gneiss (brown gneiss) are spatially associated with steeply-inclined amphibolite facies shear zones that have been interpreted as terrane boundaries. Interpretation of the primary chemical composition of these rocks is complicated by partial melting and melt loss during granulite facies metamorphism, and contamination with melts derived from the adjacent migmatitic TTG host rocks. Notwithstanding, the composition of the layered ultramafic–mafic rocks is suggestive of a protolith formed by differentiation of tholeiitic magma, where the ultramafic portions of these bodies represent the metamorphosed cumulates and the mafic portions the metamorphosed fractionated liquids. Although the composition of the brown gneiss does not clearly discriminate the protolith, it most likely represents a metamorphosed sedimentary or volcano-sedimentary sequence. For Archean rocks, particularly those metamorphosed to granulite facies, the geochemical characteristics typically used for discrimination of paleotectonic environments are neither strictly appropriate nor clearly diagnostic. Many of the rocks in the Lewisian Complex have ‘arc-like’ trace element signatures. These signatures are interpreted to reflect derivation from hydrated enriched mantle and, in the case of the TTG gneisses, partial melting of amphibolite source rocks containing garnet and a Ti-rich phase, probably rutile. However, it is becoming increasingly recognised that in Archean rocks such signatures may not be unique to a subduction environment but may relate to processes such as delamination and dripping. Consequently, it is unclear whether the Lewisian ultramafic–mafic rocks and brown gneisses represent products of plate margin or intraplate magmatism. Although a subduction-related origin is possible, we propose that an intraplate origin is equally plausible. If the second alternative is correct, the ultramafic–mafic rocks and brown gneisses may represent the remnants of intracratonic greenstone belts that sank into the deep crust due to their density contrast with the underlying partially molten low viscosity TTG orthogneisses