Estimating Bacterial diversity in scirtothrips dorsalis (thysanoptera: thripidae) Via Next generation sequencing

The last 2 decades have produced a better understanding of insect-microbial associations and yielded some important opportunities for insect control. However, most of our knowledge comes from model systems. Thrips (Thysanoptera: Thripidae) have been understudied despite their global importance as invasive species, plant pests and disease vectors. Using a culture and primer independent next-generation sequencing and metagenomics pipeline, we surveyed the bacteria of the globally important pest, Scirtothrips dorsalis Hood. The most abundant bacterial phyla identified were Actinobacteria and Proteobacteria and the most abundant genera were Propionibacterium, Stenotrophomonas, and Pseudomonas. A total of 189 genera of bacteria were identified. The absence of any vertically transferred symbiont taxa commonly found in insects is consistent with other studies suggesting that thrips primarilly acquire resident microbes from their environment. This does not preclude a possible beneficial/intimate association between S. dorsalis and the dominant taxa identified and future work should determine the nature of these associations

Epigenomic translocation of H3K4me3 broad domains over oncogenes following hijacking of super-enhancers

Chromosomal translocations are important drivers of hematological malignancies whereby proto-oncogenes are activated by juxtaposition with super-enhancers, often called enhancer hijacking. We analysed the epigenomic consequences of rearrangements between the super-enhancers of the immunoglobulin heavy locus (IGH) and proto-oncogene CCND1 that are common in B cell malignancies. By integrating BLUEPRINT epigenomic data with DNA breakpoint detection, we characterised the normal chromatin landscape of the human IGH locus and its dynamics after pathological genomic rearrangement. We detected an H3K4me3 broad domain (BD) within the IGH locus of healthy B cells that was absent in samples with IGH-CCND1 translocations. The appearance of H3K4me3-BD over CCND1 in the latter was associated with overexpression and extensive chromatin accessibility of its gene body. We observed similar cancer-specific H3K4me3-BDs associated with super-enhancer hijacking of other common oncogenes in B cell (MAF, MYC and FGFR3/NSD2) and in T-cell malignancies (LMO2, TLX3 and TAL1). Our analysis suggests that H3K4me3-BDs can be created by super-enhancers and supports the new concept of epigenomic translocation, where the relocation of H3K4me3-BDs from cell identity genes to oncogenes accompanies the translocation of super-enhancers

L-Edge Spectroscopy of Dilute, Radiation-Sensitive Systems Using a Transition-Edge-Sensor Array

We present X-ray absorption spectroscopy and resonant inelastic X-ray scattering (RIXS) measurements on the iron L-edge of 0.5 mM aqueous ferricyanide. These measurements demonstrate the ability of high-throughput transition-edge-sensor (TES) spectrometers to access the rich soft X-ray (100-2000eV) spectroscopy regime for dilute and radiation-sensitive samples. Our low-concentration data are in agreement with high-concentration measurements recorded by conventional grating-based spectrometers. These results show that soft X-ray RIXS spectroscopy acquired by high-throughput TES spectrometers can be used to study the local electronic structure of dilute metal-centered complexes relevant to biology, chemistry and catalysis. In particular, TES spectrometers have a unique ability to characterize frozen solutions of radiation- and temperature-sensitive samples.Comment: 19 pages, 4 figure

Categorizing and Defining Popular Psychological Terms Used Within the Youth Athlete Talent Development Literature: A Systematic Review

Inconsistencies in the use and definition of psychological terms within the talent development literature have been identified. To advance the scientific field, the creation of a shared language is recommended. This review aimed to systematically (i) identify terms used in empirical studies to describe psychological components purported to facilitate athletes' development; (ii) analyse definition and meanings of these terms; and (iii) group, label and define terms into meaning clusters. A systematic review using a narrative approach to synthesise information was conducted. A comprehensive literature search of SPORTDiscus, PsycINFO, PsycARTICLES, and ERIC was completed in May 2015. In total 21 empirical studies, published between 2002 and 2015, met the inclusion criteria and were included in the narrative synthesis. Thematic analysis was used to analyse the findings. Identified psychological terms were categorised as psychological skills or psychological characteristics. Psychological skills were defined as athletes’ ability to use learned psychological strategies (e.g., self-talk) to regulate and facilitate the enhancement of psychological characteristics. Psychological characteristics were defined as predispositions that impact upon athlete development (e.g., self-confidence). Despite being relatively enduring and consistent across a range of situations, psychological characteristics can be regulated and enhanced through the use of psychological skills

Upper crustal structure and axial topography at intermediate spreading ridges : seismic constraints from the southern Juan de Fuca Ridge

Author Posting. © American Geophysical Union, 2005. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 110 (2005): B12104, doi:10.1029/2005JB003630.We use multichannel seismic reflection data to image the upper crustal structure of 0-620 ka crust along the southern Juan de Fuca Ridge (JdFR). The study area comprises two segments spreading at intermediate rate with an axial high morphology with narrow (Cleft) and wide (Vance) axial summit grabens (ASG). Along most of the axis of both segments we image the top of an axial magma chamber (AMC). The AMC along Cleft deepens from south to north, from 2.0 km beneath the RIDGE Cleft Observatory and hydrothermal vents near the southern end of the segment, to 2.3 km at the northern end near the site of the 1980’s eruptive event. Along the Vance segment, the AMC also deepens from south to north, from 2.4 km to 2.7 km. Seismic layer 2A, interpreted as the basaltic extrusive layer, is 250-300 m thick at the ridge axis along the Cleft segment, and 300-350 m thick along the axis of the Vance segment. However off-axis layer 2A is similar in both segments (500-600 m), indicating ~90% and ~60% off-axis thickening at the Cleft and Vance segments, respectively. Half of the thickening occurs sharply at the walls of the ASG, with the remaining thickening occurring within 3-4 km of the ASG. Along the full length of both segments, layer 2A is thinner within the ASG, compared to the ridge flanks. Previous studies argued that the ASG is a cyclic feature formed by alternating periods of magmatism and tectonic extension. Our observations agree with the evolving nature of the ASG. However, we suggest that its evolution is related to large changes in axial morphology produced by small fluctuations in magma supply. Thus the ASG, rather than being formed by excess volcanism, is a rifted flexural axial high. The changes in axial morphology affect the distribution of lava flows along the ridge flanks, as indicated by the pattern of layer 2A thickness. The fluctuations in magma supply may occur at all spreading rates, but its effects on crustal structure and axial morphology are most pronounced along intermediate spreading rate ridges.This study was supported by the National Science Foundation grants OCE-0002551 to Woods Hole Oceanographic Institution, OCE-0002488 to Lamont-Doherty Earth Observatory, and OCE-0002600 to Scripps Institution of Oceanography

Seismic structure of the Endeavour Segment, Juan de Fuca Ridge : correlations with seismicity and hydrothermal activity

Author Posting. © American Geophysical Union, 2007. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 112 (2007): B02401, doi:10.1029/2005JB004210.Multichannel seismic reflection data collected in July 2002 at the Endeavour Segment, Juan de Fuca Ridge, show a midcrustal reflector underlying all of the known high-temperature hydrothermal vent fields in this area. On the basis of the character and geometry of this reflection, its similarity to events at other spreading centers, and its polarity, we identify this as a reflection from one or more crustal magma bodies rather than from a hydrothermal cracking front interface. The Endeavour magma chamber reflector is found under the central, topographically shallow section of the segment at two-way traveltime (TWTT) values of 0.9–1.4 s (∼2.1–3.3 km) below the seafloor. It extends approximately 24 km along axis and is shallowest beneath the center of the segment and deepens toward the segment ends. On cross-axis lines the axial magma chamber (AMC) reflector is only 0.4–1.2 km wide and appears to dip 8–36° to the east. While a magma chamber underlies all known Endeavour high-temperature hydrothermal vent fields, AMC depth is not a dominant factor in determining vent fluid properties. The stacked and migrated seismic lines also show a strong layer 2a event at TWTT values of 0.30 ± 0.09 s (380 ± 120 m) below the seafloor on the along-axis line and 0.38 ± 0.09 s (500 ± 110 m) on the cross-axis lines. A weak Moho reflection is observed in a few locations at TWTT values of 1.9–2.4 s below the seafloor. By projecting hypocenters of well-located microseismicity in this region onto the seismic sections, we find that most axial earthquakes are concentrated just above the magma chamber and distributed diffusely within this zone, indicating thermal-related cracking. The presence of a partially molten crustal magma chamber argues against prior hypotheses that hydrothermal heat extraction at this intermediate spreading ridge is primarily driven by propagation of a cracking front down into a frozen magma chamber and indicates that magmatic heat plays a significant role in the hydrothermal system. Morphological and hydrothermal differences between the intermediate spreading Endeavour and fast spreading ridges are attributable to the greater depth of the Endeavour AMC and the corresponding possibility of axial faulting.E.V.A. was supported by a National Science Foundation Graduate Research Fellowship, the WHOI-MIT Joint Program, and the WHOI Deep Ocean Exploration Institute. This work was also supported by OCE-0002551 to the Woods Hole Oceanographic Institution, OCE-0002488 to Lamont-Doherty Earth Observatory, and OCE-0002600 to Scripps Institution of Oceanography

Constructing the crust along the Galapagos Spreading Center 91.3°–95.5°W : correlation of seismic layer 2A with axial magma lens and topographic characteristics

Author Posting. © American Geophysical Union, 2004. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 109 (2004): B10310, doi:10.1029/2004JB003066.Multichannel seismic reflection data are used to infer crustal accretion processes along the intermediate spreading Galapagos Spreading Center. East of 92.5°W, we image a magma lens beneath the ridge axis that is relatively shallow (1.0–2.5 km below the seafloor) and narrow (∼0.5–1.5 km, cross-axis width). We also image a thin seismic layer 2A (0.24–0.42 km) that thickens away from the ridge axis by as much as 150%. West of 92.7°W, the magma lens is deeper (2.5–4.5 km) and wider (0.7–2.4 km), and layer 2A is thicker (0.36–0.66 km) and thickens off axis by <40%. The positive correlation between layer 2A thickness and magma lens depth supports the interpretation of layer 2A as the extrusive volcanic layer with thickness controlled by the pressure on the magma lens and its ability to push magma to the surface. Our findings also suggest that narrower magma lenses focus diking close the ridge axis such that lava flowing away from the ridge axis will blanket older flows and thicken the extrusive crust off axis. Flow of lava away from the ridge axis is probably promoted by the slope of the axial bathymetric high, which is largest east of 92.5°W. West of ∼94°W the “transitional” axial morphology lacks a prominent bathymetric high and layer 2A no longer thickens off axis. We detect no magma lens west of 94.7°W where a small axial valley appears. The above changes can be linked to the westward decrease in the magma and heat flux associated with the fading influence of the Galapagos hot spot on the Galapagos Spreading Center.This project was funded by NSF-OCE- 0002189