Microstructure and optical properties of Pr3+-doped hafnium silicate films

International audienceIn this study, we report on the evolution of the microstructure and photoluminescence properties of Pr 3+-doped hafnium silicate thin films as a function of annealing temperature (T A). The composition and microstructure of the films were characterized by means of Rutherford backscattering spectrometry, spectroscopic ellipsometry, Fourier transform infrared absorption, and X-ray diffraction, while the emission properties have been studied by means of photoluminescence (PL) and PL excitation (PLE) spectroscopies. It was observed that a post-annealing treatment favors the phase separation in hafnium silicate matrix being more evident at 950°C. The HfO 2 phase demonstrates a pronounced crystallization in tetragonal phase upon 950°C annealing. Pr 3+ emission appeared at T A = 950°C, and the highest efficiency of Pr 3+ ion emission was detected upon a thermal treatment at 1,000°C. Analysis of the PLE spectra reveals an efficient energy transfer from matrix defects towards Pr 3+ ions. It is considered that oxygen vacancies act as effective Pr 3+ sensitizer. Finally, a PL study of undoped HfO 2 and HfSiO x matrices is performed to evidence the energy transfer

Onchocerca jakutensis Filariasis in Humans

We identified Onchocerca jakutensis as the causative agent of an unusual human filariasis in a patient with lupus erythematosus. To our knowledge, this is the first case of human infection with O. jakutensis and the first human case of zoonotic onchocercosis involving >1 worm

A procedure for the change point problem in parametric models based on phi-divergence test-statistics

This paper studies the change point problem for a general parametric, univariate or multivariate family of distributions. An information theoretic procedure is developed which is based on general divergence measures for testing the hypothesis of the existence of a change. For comparing the accuracy of the new test-statistic a simulation study is performed for the special case of a univariate discrete model. Finally, the procedure proposed in this paper is illustrated through a classical change-point example

How to Identify and Separate Bright Galaxy Clusters from the Low-frequency Radio Sky?

In this work we simulate the $50-200$ MHz radio sky that is constrained in the field of view ($5^{\circ}$ radius) of the 21 Centimeter Array (21CMA), by carrying out Monte-Carlo simulations to model redshifted cosmological reionization signals and strong contaminating foregrounds, including emissions from our Galaxy, galaxy clusters, and extragalactic point sources. As an improvement of previous works, we consider in detail not only random variations of morphological and spectroscopic parameters within the ranges allowed by multi-band observations, but also evolution of radio halos in galaxy clusters, assuming that relativistic electrons are re-accelerated in the ICM in merger events and lose energy via both synchrotron emission and inverse Compton scattering with CMB photons. By introducing a new approach designed on the basis of independent component analysis (ICA) and wavelet detection algorithm, we prove that, with a cumulative observation of one month with the 21CMA array, about $80\%$ of galaxy clusters with central brightness temperatures of $> 10~{\rm K}$ at 65 MHz can be safely identified and separated from the overwhelmingly bright foreground. We find that the morphological and spectroscopic distortions are extremely small as compared to the input simulated clusters, and the reduced $\chi^2$ of brightness temperature profiles and spectra are controlled to be $\lesssim 0.5$ and $\lesssim 1.3$, respectively. These results robustly indicate that in the near future a sample of dozens of bright galaxy clusters will be disentangled from the foreground in 21CMA observations, the study of which will greatly improve our knowledge about cluster merger rates, electron acceleration mechanisms in cluster radio halos, and magnetic field in the ICM.Comment: 35 pages, 10 figures, Accepted for publication in The Astrophysical Journa

Effect of storage temperature on the stability of spray dried bacteriophage powders

This study aimed to assess the robustness of using a spray drying approach and formulation design in producing inhalable phage powders. Two types of Pseudomonas phages, PEV2 (Podovirus) and PEV40 (Myovirus) in two formulations containing different amounts of trehalose (70% and 60%) and leucine (30% and 40%) were studied. Most of the surface of the produced powders was found to be covered in crystalline leucine. The powders were stored at 4 °C and 20 °C under vacuum. The phage stability and in vitro aerosol performance of the phage powders were examined on the day of production and after 1, 3 and 12 months of storage. A minor titer loss during production was observed for both phages (0.2–0.8 log10 pfu/ml). The storage stability of the produced phage powders was found to be phage and formulation dependent. No further reduction in titer occurred for PEV2 powders stored at 4 °C across the study. The formulation containing 30% leucine maintained the viability of PEV2 at 20 °C, while the formulation containing 40% leucine gradually lost titer over time with a storage reduction of ∼0.9 log10 pfu/ml measured after 12 months. In comparison, the PEV40 phage powders generally had a ∼ 0.5 log10 pfu/ml loss upon storage regardless of temperature. When aerosolized, the total in vitro lung doses of PEV2 were of the order of 107 pfu, except the formulation containing 40% leucine stored at 20 °C which had a lower lung dose. The PEV40 powders also had lung doses of 106–107 pfu. The results demonstrate that spray dried Myoviridae and Podoviridae phage in a simple formulation of leucine and trehalose can be successfully stored for one year at 4 °C and 20 °C with vacuum packaging.The University of Sydney; Australian Research Council; National Institute of Allergy and Infectious Diseases of the National Institutes of Health; National Health and Medical Research Council Centre of Research Excellence in Tuberculosis Contro

Interpreting Attoclock Measurements of Tunnelling Times

Resolving in time the dynamics of light absorption by atoms and molecules, and the electronic rearrangement this induces, is among the most challenging goals of attosecond spectroscopy. The attoclock is an elegant approach to this problem, which encodes ionization times in the strong-field regime. However, the accurate reconstruction of these times from experimental data presents a formidable theoretical challenge. Here, we solve this problem by combining analytical theory with ab-initio numerical simulations. We apply our theory to numerical attoclock experiments on the hydrogen atom to extract ionization time delays and analyse their nature. Strong field ionization is often viewed as optical tunnelling through the barrier created by the field and the core potential. We show that, in the hydrogen atom, optical tunnelling is instantaneous. By calibrating the attoclock using the hydrogen atom, our method opens the way to identify possible delays associated with multielectron dynamics during strong-field ionization.Comment: 33 pages, 10 figures, 3 appendixe

Ecology and conservation of polylepis forests: What do we know and what do we ignore?

Los bosques de Polylepis, endémicos de las montañas de Sudamérica, suelen estar asociados con sitios relativamente inaccesibles como quebradas, roquedales o laderas empinadas. Las hipótesis más discutidas que explican esta asociación son: 1) factores abióticos como la humedad, la temperatura o el viento, ó 2) factores antropogénicos como la protección del ramoneo por ganado doméstico, la tala o los fuegos iniciados por el ser humano. Para resumir el estado de avance en el conocimiento, realizamos una revisión cuantitativa sobre los estudios de ecología y conservación de bosques de Polylepis a lo largo de toda su distribución. Identificamos 139 publicaciones relevantes, de las que el 36% estuvo concentrado en la Argentina. Las publicaciones incluyen 20 de las ~27 especies existentes, de las cuales las más estudiadas son P. australis y P. tarapacana, con 45 y 21 publicaciones, respectivamente. En el otro extremo, hay 7 especies no representadas en publicaciones. Las disciplinas más populares fueron la ecología de poblaciones (35%) y los estudios sobre biodiversidad y comunidades (18%). Muy pocos estudios se repiten de una región a la otra. Los estudios que intentan comprender la contribución relativa de factores abióticos vs. antropogénicos para explicar la asociación de los bosques con lugares relativamente inaccesibles señalan una contribución mayor de los factores antrópicos, aunque todos están concentrados en el extremo sur de la distribución de Polylepis en la Argentina. Sugerimos áreas de vacancia en estudios y posibles mejoras en la producción de nuevas publicaciones y en la red de interacciones entre investigadores interesados en los ecosistemas de Polylepis.Polylepis forests, endemic to the Andes of South America, are generally associated to relatively inaccessible sites such as ravines, rocky or steep slopes. The reasons for this association have been widely debated and could be of abiotic origin such as humidity, temperature or winds, or due to anthropogenic origin such as protection from browsing by domestic cattle, felling or human ignited fires. We performed a quantitative review on the ecology and conservation of Polylepis forests with the objective of summarizing the state of the art in this topic. We identified 139 relevant publications; 36% were concentrated in Argentina. The publications include 20 of the ~27 existing species; the most studied were P. australis and P. tarapacana, with 45 and 21 publications, respectively. In the opposite extreme, there were 7 species without publications. The most popular disciplines were population ecology (35%) and studies on biodiversity and communities (18%). Very few studies of the same type were repeated from one region to the other. The studies that attempt to quantify the relative contribution of abiotic and anthropogenic factors in restricting forest to inaccessible sites show a stronger contribution of human impact, but are all concentrated in the south of distribution of Polylepis forest in Argentina. We suggest research vacancy areas and possible improvements in the production of new publications and in the network of interactions between researchers interested in Polylepis ecosystems.Fil: Renison, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Ecología y Recursos Naturales Renovables; ArgentinaFil: Morales, Laura. University of California at Davis; Estados UnidosFil: Cuyckens, Griet An Erica. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Centro de Estudios Ambientales Territoriales y Sociales; Argentina. Universidad Nacional de Jujuy. Instituto de Ecorregiones Andinas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Ecorregiones Andinas; ArgentinaFil: Sevillano, Cristian Steven. Cornell University; Estados UnidosFil: Cabrera Amaya, Diego M.. Jardín Botánico de Bogotá José Celestino Mutis; Colombi

An optimized method for the extraction of bacterial mRNA from plant roots infected with Escherichia coli O157:H7

Analysis of microbial gene expression during host colonization provides valuable information on the nature of interaction, beneficial or pathogenic, and the adaptive processes involved. Isolation of bacterial mRNA for in planta analysis can be challenging where host nucleic acid may dominate the preparation, or inhibitory compounds affect downstream analysis, e.g., quantitative reverse transcriptase PCR (qPCR), microarray, or RNA-seq. The goal of this work was to optimize the isolation of bacterial mRNA of food-borne pathogens from living plants. Reported methods for recovery of phytopathogen-infected plant material, using hot phenol extraction and high concentration of bacterial inoculation or large amounts of infected tissues, were found to be inappropriate for plant roots inoculated with Escherichia coli O157:H7. The bacterial RNA yields were too low and increased plant material resulted in a dominance of plant RNA in the sample. To improve the yield of bacterial RNA and reduce the number of plants required, an optimized method was developed which combines bead beating with directed bacterial lysis using SDS and lysozyme. Inhibitory plant compounds, such as phenolics and polysaccharides, were counteracted with the addition of high-molecular-weight polyethylene glycol and hexadecyltrimethyl ammonium bromide. The new method increased the total yield of bacterial mRNA substantially and allowed assessment of gene expression by qPCR. This method can be applied to other bacterial species associated with plant roots, and also in the wider context of food safety

Ipsilateral vagotomy to unilaterally ovariectomized pre-pubertal rats modifies compensatory ovarian responses

The present study evaluates the participation of the vagus nerve in pre-pubertal rats with unilateral ovariectomy on puberty onset, and on progesterone, testosterone and estradiol serum levels, and the compensatory responses of the ovary. Unilateral vagotomy did not modify the onset of puberty in unilaterally ovariectomized rats. Ovulation rates of animals with the left vagus nerve sectioned and the left ovary in-situ was lower than in rats with only unilateral ovariectomy. Sectioning the left vagus to 32-day old rats with the left ovary in-situ resulted in lower compensatory ovarian hypertrophy than in rats with right unilateral ovariectomy. Twenty-eight or 32-day old animals with sectioning of the right vagus nerve and the right ovary in situ showed higher compensatory ovulation. Twenty-eight -day old rats with the right ovary in situ had higher progesterone and testosterone levels than animals of the same age with the left ovary in-situ. Compared to animals with the right ovary in situ, animals treated at 32-days of age, sectioning the ipsi-lateral vagus nerve resulted in higher progesterone levels. Higher progesterone levels were observed in 28- and 32 days old rats with the left ovary in situ and left vagus nerve sectioned. Thirty-two day old animals with the right ovary in situ and right vagus nerve sectioned had higher progesterone levels than rats of the same age with the left ovary in situ and left vagus nerve sectioned. Left vagotomy to 28-day old rats with the left ovary in situ resulted in higher testosterone levels, a reverse response to that observed in animals with sectioning of the right vagus and the right ovary in situ. Thirty-two day old rats with the left ovary in situ and left vagus nerve sectioned showed lower testosterone levels than animals without vagotomy and with the left ovary in situ

Anthropometry, dietary intake, physical activity and sitting time patterns in adolescents aged 15-17 years: an international comparison in eight Latin American countries

BACKGROUND: Although there is high prevalence of obesity and other cardiovascular risk factors among Latin American adolescents, there is limited evidence on dietary intake and physical activity (PA) patterns in this population. Therefore, we characterized anthropometry, dietary intake, PA and sitting time (ST) in adolescents aged 15-17 years from eight Latin American countries. METHODS: Six hundred seventy-one adolescents (41.4% girls) from the Latin American Study of Nutrition and Health (ELANS) were included. Nutritional status was classified by four BMI (kg/m2) categories. Waist circumference (WC) was categorized as above or below thresholds. Dietary intake was assessed through two non-consecutive 24-h dietary recalls. PA and ST were measured using the International Physical Activity Questionnaire (IPAQ). We calculated overall and country-specific estimates by sex and tested for differences between boys and girls. RESULTS: Differences in the prevalence of overweightness (15.1 and 21.6%) and obesity (8.5 and 6.5%) between boys and girls, respectively, were statistically insignificant (p = 0.059). Average energy intake was 2289.7 kcal/day (95% CI: 2231-2350) for boys and 1904.2 kcal/day (95% CI: 1840-1963) for girls (p  0.05 for all outcomes). There was no statistically significant difference in the prevalence of total energy (TE) saturated fat and added sugar (>10% of TE) between girls and boys (49.6% versus 44.8 and 81.7% versus 76.1%, respectively). Prevalence of physical inactivity was 19% in boys and 43.7% in girls (p  0.05). CONCLUSIONS: These findings highlight the high prevalence of poor dietary intake and physical inactivity in adolescents from Latin American countries. Therefore, effective and sustainable strategies and programmes are needed that promote healthier diets, regular PA and reduce ST among Latin American adolescents. TRIAL REGISTRATION: Clinical Trials NCT02226627. Retrospectively registered on August 27, 2014