Expression of human interferon gamma in Brassica napus seeds

Expressions of heterologous proteins in suitable plant tissues and targeting it into subcellular compartments using specific signals have been studied. Seed-based platforms are among those that allow recombinant proteins to stably accumulate at a relatively high concentration in a compact biomass. In this study, we used seed specific promoter (Napin) and C-terminal KDEL sequence to express human therapeutic protein, interferon gamma (IFN_y) in Brassica napus seeds. Kozak sequence was linked to the 5' end of the IFN_y gene to increase the level of expression. The constructed cassette was transformed into rapeseed. Presence and expression of the transgene were confirmed in the transformants by polymerase chain reaction (PCR) and sodium dodecylsulfatepolyacrylamide gel electrophoresis (SDS-PAGE). Analysis of transgenic plants by enzyme-linked immunosorbent assay (ELISA), dot blot and western blot indicated that IFN_y protein is being expressed in B. napus seeds and is as active as the standard IFN_y. Our results indicate that plant seeds have tremendous potential for production of recombinant proteins as ‘natural bioreactors’.Key words: Interferon gamma, KDEL retention signal, seed specific promoter, Brassica napus, recombinant proteins

High Performance Control of Grid Connected Cascaded H-Bridge Active Rectifier Based on Type II-Fuzzy Logic Controller with Low Frequency Modulation Technique

This paper tries to employ a fuzzy logic (FL) controller type II to control the Cascaded H-Bridge (CHB) active rectifier. This controller has strong performance, specially, when a low switching frequency Selective Harmonic Elimination (SHE) method is used. In order to regulate all of the DC link voltages, the optimum voltage balancing strategy in the low frequency modulation technique is used in the proposed method. Finally, the performance and effectiveness of the proposed method is validated in MATLAB environment.   All the simulation and result has been simulated by MATLAB software

A critical review of bioactive glasses and glass–ceramics in cancer therapy

There is an ongoing profound shift in using glass as a primarily passive material to one that instills active properties. We believe and demonstrate that bioactive glasses (BGs) and glass–ceramics (BGCs) as functional biomaterials for cancer therapy can transform the world of healthcare in the 21st century. Melt/gel-derived BGs and BGCs can carry many exotic elements, including less common rare-earth, and trigger highly efficient anticancer properties via the combination of radiotherapy, photothermal therapy, magnetic hyperthermia, along with drug or therapeutic ions delivery. The addition of these dopants modifies the bioactivity, imparts novel functionalities, and induces specific biological effects that are not achievable using other classes of biomaterials. In this paper, we have briefly reviewed and discussed the current knowledge on promising compositions, processing parameters, and applications of BGs and BGCs in treating cancer. We also envisage the need for further research on this particular, unique class of BGs and BGCs

Algorithms for Art Gallery Illumination

We consider a variant of the Art Gallery Problem, where a polygonal region is to be covered with light sources, with light fading over distance. We describe two practical algorithms, one based on a discrete approximation, and another based on nonlinear programming by means of simplex partitioning strategies. For the case where the light positions are given, we describe a fully polynomial-time approximation scheme. For both algorithms we present an experimental evaluation

Unital Design Based Sink Location Service for Wireless Sensor Networks

In wireless sensor networks (WSNs), providing source node with sink position is an essential principle for geographic routing protocols. Previous works have only focused on the problem of sink localization in a 2-D sensing field while that of 3-D WSNs has received little attention. Providing sink location service with low control overhead and energy consumption is a challenging issue in 3-D WSNs area. In this paper, we propose a unital design based sink location service (UDSL) for WSNs. In this scheme, sink location announcement (SLA) and sink location query (SLQ) packets are forwarded along two paths or blocks. The node located at the intersection of the two paths sends the sink position to the source. In the proposed method, SLA and SLQ messages are constructed using unital design blocks. For this purpose, a mapping from unital design to sink location service has been proposed. However, this basic mapping does not guarantee an intersection of paths,therefore, we propose an enhanced UDSL providing 100 probability of intersection. In order to analyze the proposed scheme's performance, extensive WSNs simulations and experiments have been conducted. The results indicate that UDSL provides reasonable performance in terms of hop counts, path length, and energy consumption for providing sink location service. © 2013 IEEE

Measurements of scattering observables for the pdpd break-up reaction

High-precision measurements of the scattering observables such as cross sections and analyzing powers for the proton-deuteron elastic and break-up reactions have been performed at KVI in the last two decades and elsewhere to investigate various aspects of the three-nucleon force (3NF) effects simultaneously. In 2006 an experiment was performed to study these effects in $\vec{p}+d$ break-up reaction at 135 MeV with the detection system, Big Instrument for Nuclear polarization Analysis, BINA. BINA covers almost the entire kinematical phase space of the break-up reaction. The results are interpreted with the help of state-of-the-art Faddeev calculations and are partly presented in this contribution.Comment: Proceedings of 19th International IUPAP Conference on Few-Body Problems in Physics, Bonn University, 31.08 - 05.09.2009, Bonn, GERMAN

The Effects of Oxytocin on Withdrawal, Craving and Stress Response in Heroin-Dependent Patients: A Randomized, Double-Blind Clinical Trial

Opioid dependence is an increasing clinical and public health problem. Current pharmacotherapies have limited efficacy and cause serious side effects. Increasing bodies of evidences suggest the neuropeptide, oxytocin (OT), as a potential treatment for drug abuse disorders. The current study was designed to evaluate the effect of OT on withdrawal, craving and anxiety scores, cortisol and dehydroepiandrosterone sulphate (DHEAS) blood level in heroin-dependent male patients. This randomized, double-blind placebo-controlled clinical trial was conducted on 58 males with opioid dependence by Abstinence Center of Addictive People in Iran. The participants were randomly allocated to receive intranasal OT (single dose; 40 IU, n = 29) or placebo (n = 29). Heroine withdrawal, craving and anxiety scores were measured using the Opioid Withdrawal Scale, Visual Analogue Scale and (Desire for Drug Questionnaire), and Hamilton checklist respectively. The cortisol and DHEAS levels at baseline and different post-intervention time were measured using a competitive immunoanalysis method. Acute OT administration reduced craving and withdrawal scores but did not change anxiety significantly. Single dose of OT decreased the level of cortisol and improved the cortisol/DHEAS ratio in the heroin users during abstinence (p < 0.01). These results suggest that OT may be useful in the attenuation of craving, withdrawal symptom in heroin-dependent patients and might be considered a new potential treatment for heroin dependence where positive effects of OT on stress-related hormones may be involved in this effect of OT. © 2019 S. Karger AG, Basel. Copyright: All rights reserved