Structure of the Mg-Chelatase Cofactor GUN4 Reveals a Novel Hand-Shaped Fold for Porphyrin Binding

In plants, the accumulation of the chlorophyll precursor Mg-protoporphyrin IX (Mg-Proto) in the plastid regulates the expression of a number of nuclear genes with functions related to photosynthesis. Analysis of the plastid-to-nucleus signaling activity of Mg-Proto in Arabidopsis thaliana led to the discovery of GUN4, a novel porphyrin-binding protein that also dramatically enhances the activity of Mg-chelatase, the enzyme that synthesizes Mg-Proto. GUN4 may also play a role in both photoprotection and the cellular shuttling of tetrapyrroles. Here we report a 1.78-Å resolution crystal structure of Synechocystis GUN4, in which the porphyrin-binding domain adopts a unique three dimensional fold with a “cupped hand” shape. Biophysical and biochemical analyses revealed the specific site of interaction between GUN4 and Mg-Proto and the energetic determinants for the GUN4 • Mg-Proto interaction. Our data support a novel protective function for GUN4 in tetrapyrrole trafficking. The combined structural and energetic analyses presented herein form the physical-chemical basis for understanding GUN4 biological activity, including its role in the stimulation of Mg-chelatase activity, as well as in Mg-Proto retrograde signaling

Triple point cancelling numbers of surface links and quandle cocycle invariants

The unknotting or triple point cancelling number of a surface link F is the least number of 1-handles for F such that the 2-knot obtained from F by surgery along them is unknotted or pseudo-ribbon, respectively. These numbers have been often studied by knot groups and Alexander invariants. On the other hand, quandle colorings and quandle cocycle invariants of surface links were introduced and applied to other aspects, including non-invertibility and triple point numbers. In this paper, we give lower bounds of the unknotting or triple point cancelling numbers of surface links by using quandle colorings and quandle cocycle invariants

Cationic M2L4 Cages for Perchlorate Removal from Aqueous Solutions and Preferential Perchlorate Incorporation in Hydrophilic Solutions

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