Strong enhancement of two-photon absorption properties in synergic 'semi-disconnected' multiporphyrin assemblies designed for combined imaging and photodynamic therapy.

International audienceThe synthesis and photophysical properties of new multiporphyrin assemblies are described. Their design, based on a smooth electronic disconnection between two-photon absorbing (2PA) octupolar or quadrupolar cores and the peripheral porphyrins, leads to a major increase in (non-resonant) 2PA responses in the NIR, while fully retaining the fluorescence and photosensitization properties of isolated porphyrins. This approach, which involves electronic coupling of semi-disconnected moieties in the higher excited states of the synergic systems, is of interest to fully benefit from the advantages of selective 2PA for application in combined two-photon high resolution imaging and photodynamic therapy

Ion flux, transmembrane potential, and osmotic stabilization: A new electrophysiological dynamic model for Eukaryotic cells

International audienceSurvival of mammalian cells is achieved by tight control of cell volume while transmembrane potential is known to control many cellular functions since the seminal work of Hodgkin and Huxley. Regulation of cell volume and transmembrane potential have a wide range of implications in physiology, from neurological and cardiac disorders to cancer and muscle fatigue. Therefore understanding the relationship between transmembrane potential, ion fluxes, and cell volume regulation has become of great interest. In this paper we derive a system of differential equations that links transmembrane potential, ionic concentrations, and cell volume. This model demonstrates that volume stabilization occurs within minutes of changes in extracellular osmotic pressure. We infer a straightforward relationship between transmembrane potential and cell volume. Our model is a generalization of previous models in which either cell volume was constant or osmotic regulation instantaneous. When the extracellular osmotic pressure is constant, the cell volume varies as a function of transmembrane potential and ions fluxes thus providing an implicit link between transmembrane potential and cell growth. Numerical simulations of the model provide results that are consistent with experimental data in terms of time-related changes in cell volume and dynamics of the phenomena

Estimation and Uncertainty Assessment of Surface Microclimate Indicators at Local Scale Using Airborne Infrared Thermography and Multispectral Imagery

A precise estimation and the characterization of the spatial variability of microclimate conditions (MCCs) are essential for risk assessment and site-specific management of vector-borne diseases and crop pests. The objective of this study was to estimate at local scale, and assess the uncertainties of Surface Microclimate Indicators (SMIs) derived from airborne infrared thermography and multispectral imaging. SMIs including Surface Temperature (ST) were estimated in southern Quebec, Canada. The formulation of their uncertainties was based on in-situ observations and the law of propagation of uncertainty. SMIs showed strong local variability and intra-plot variability of MCCs in the study area. The ST values ranged from 290 K to 331 K. They varied more than 17 K on vegetable crop fields. The correlation between ST and in-situ observations was very high (r = 0.99, p = 0.010). The uncertainty and the bias of ST compared to in-situ observations were 0.73 K and ±1.42 K respectively. This study demonstrated that very high spatial resolution multispectral imaging and infrared thermography present a good potential for the characterization of the MCCs that govern the abundance and the behavior of disease vectors and crop pests in a given area

Amplification dynamics of miniature inverted-repeat transposable elements and their impact on rice trait variability

Ministerio de Ciencia y Innovación (PID2019-106374RB-I00) - DOI 10.13039/501100011033Transposable elements (TEs) are a rich source of genetic variability. Among TEs, miniature inverted-repeat TEs (MITEs) are of particular interest as they are present in high copy numbers in plant genomes and are closely associated with genes. MITEs are deletion derivatives of class II transposons, and can be mobilized by the transposases encoded by the latter through a typical cut-and-paste mechanism. However, MITEs are typically present at much higher copy numbers than class II transposons. We present here an analysis of 103 109 transposon insertion polymorphisms (TIPs) in 738 Oryza sativa genomes representing the main rice population groups. We show that an important fraction of MITE insertions has been fixed in rice concomitantly with its domestication. However, another fraction of MITE insertions is present at low frequencies. We performed MITE TIP-genome-wide association studies (TIP-GWAS) to study the impact of these elements on agronomically important traits and found that these elements uncover more trait associations than single nucleotide polymorphisms (SNPs) on important phenotypes such as grain width. Finally, using SNP-GWAS and TIP-GWAS we provide evidence of the replicative amplification of MITEs

European H2020 Project WORTECS Wireless Mixed Reality Prototyping

This paper presents European collaborative project WORTECS objectives and reports on the development of several radio and optical wireless prototypes and a demonstrator targeting mixed reality (MR) application. The aim is to achieve a net throughput of up to Tbps in an indoor heterogeneous network for the MR use case, which seems to be a high throughput "killer application" beyond 5G. A special routing device is associated with the demonstrator to select the most suitable wireless access technology. Post introduction to the project, an overview of the demonstrator is presented with details of the current progress of the prototypes

Electric pulses: a flexible tool to manipulate cytosolic calcium concentrations and generate spontaneous-like calcium oscillations in mesenchymal stem cells

Human adipose mesenchymal stem cells (haMSCs) are multipotent adult stem cells of great interest in regenerative medicine or oncology. They present spontaneous calcium oscillations related to cell cycle progression or differentiation but the correlation between these events is still unclear. Indeed, it is difficult to mimic haMSCs spontaneous calcium oscillations with chemical means. Pulsed electric fields (PEFs) can permeabilise plasma and/or organelles membranes depending on the applied pulses and therefore generate cytosolic calcium peaks by recruiting calcium from the external medium or from internal stores. We show that it is possible to mimic haMSCs spontaneous calcium oscillations (same amplitude, duration and shape) using 100 μs PEFs or 10 ns PEFs. We propose a model that explains the experimental situations reported. PEFs can therefore be a flexible tool to manipulate cytosolic calcium concentrations. This tool, that can be switched on and off instantaneously, contrary to chemicals agents, can be very useful to investigate the role of calcium oscillations in cell physiology and/or to manipulate cell fate

A microfluidic biochip for the nanoporation of living cells

International audienceThis paper deals with the development of a microfluidic biochip for the exposure of living cells to nanosecond pulsed electric fields (nsPEF). When exposed to ultra short electric pulses (typical duration of 3-10ns), disturbances on the plasma membrane and on the intra cellular components occur, modifying the behavioral response of cells exposed to drugs or transgene vectors. This phenomenon permits to envision promising therapies. The presented biochip is composed of thick gold electrodes that are designed to deliver a maximum of energy to the biological medium containing cells. The temporal and spectral distributions of the nsPEF are considered for the design of the chip. In order to validate the fabricated biochip ability to orient the pulse towards the cells flowing within the exposition channels, a frequency analysis is provided. High voltage measurements in the time domain are performed to characterize the amplitude and the shape of the nsPEF within the exposition channels and compared to numerical simulations achieved with a 3D Finite-Difference Time-Domain code. We demonstrate that the biochip is adapted for 3 ns and 10 ns pulses and that the nsPEF are homogenously applied to the biological cells regardless their position along the microfluidic channel. Furthermore, biological tests performed on the developed microfluidic biochip permit to prove its capability to permeabilize living cells with nanopulses. To the best of our knowledge, we report here the first successful use of a microfluidic device optimized for the achievement and real time observation of the nanoporation of living cells

Disaggregation of SMOS soil moisture to 100m resolution using MODIS optical/thermal and sentinel-1 radar data: evaluation over a bare soil site in morocco

The 40 km resolution SMOS (Soil Moisture and Ocean Salinity) soil moisture, previously disaggregated at a 1 km resolution using the DISPATCH (DISaggregation based on Physical And Theoretical scale CHange) method based on MODIS optical/thermal data, is further disaggregated to 100 m resolution using Sentinel-1 backscattering coefficient (σ°). For this purpose, three distinct radar-based disaggregation methods are tested by linking the spatio-temporal variability of σ° and soil moisture data at the 1 km and 100 m resolution. The three methods are: (1) the weight method, which estimates soil moisture at 100 m resolution at a certain time as a function of σ° ratio (100 m to 1 km resolution) and the 1 km DISPATCH products of the same time; (2) the regression method which estimates soil moisture as a function of σ° where the regression parameters (e.g., intercept and slope) vary in space and time; and (3) the Cumulative Distribution Function (CDF) method, which estimates 100 m resolution soil moisture from the cumulative probability of 100 m resolution backscatter and the maximum to minimum 1 km resolution (DISPATCH) soil moisture difference. In each case, disaggregation results are evaluated against in situ measurements collected between 1 January 2016 and 11 October 2016 over a bare soil site in central Morocco. The determination coefficient (R2) between 1 km resolution DISPATCH and localized in situ soil moisture is 0.31. The regression and CDF methods have marginal effect on improving the DISPATCH accuracy at the station scale with a R2 between remotely sensed and in situ soil moisture of 0.29 and 0.34, respectively. By contrast, the weight method significantly improves the correlation between remotely sensed and in situ soil moisture with a R2 of 0.52. Likewise, the soil moisture estimates show low root mean square difference with in situ measurements (RMSD = 0.032 m3 m−3).This work is a contribution to the REC project funded by the European Commission Horizon 2020 Programme for Research and Innovation (H2020) in the context of the Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) action under grant agreement no: 645642. In addition, this work has been partially funded by a public grant of Ministerio de Economía y Competitividad (DI-14-06587) and AGAUR-Generalitat de Catalunya (DI-2015-058)

Modeling actual water use under different irrigation regimes at district scale: Application to the FAO-56 dual crop coefficient method

The modeling of irrigation in land surface models are generally based on two soil moisture parameters SMthreshold and SMtarget at which irrigation automatically starts and stops, respectively. Typically, both parameters are usually set to optimal values allowing to fill the soil water reservoir with just the estimated right amount and to avoid crop water excess at all times. The point is that agricultural practices greatly vary according to many factors (climatological, crop, soil, technical, human, etc.). To fill the gap, we propose a new calibration method of SMthreshold and SMtarget to represent the irrigation water use in any (optimal, deficit or even over) irrigation regime. The approach is tested using the dual-crop coefficient FAO-56 model implemented at the field scale over an 8100 ha irrigation district in northeastern Spain where the irrigation water use is precisely monitored at the district scale. Both irrigation parameters are first retrieved at monthly scale from the irrigation observations of year 2019. The irrigation simulated by the FAO-56 model is then evaluated against observations at district and weekly scale over 5 years (2017–2021) separately. The performance of the newly calibrated irrigation module is also assessed by comparing it against three other modules with varying configurations including default estimates for SMthreshold and SMtarget. The proposed irrigation module obtains systematically the best performance for each of the 5 years with an overall correlation coefficient of 0.95 ± 0.02 and root-mean square error of 0.27 ± 0.07 hm3/week (0.64 ± 0.17 mm/day). Unlike the three irrigation modules used as benchmark, the new irrigation module is able to reproduce the farmers’ practices throughout the year, and especially, to simulate the actual water use in the deficit and excess irrigation regimes occurring in the study area in spring and summer, respectively.This study was supported by the IDEWA project ( ANR-19-P026-003 ) of the Partnership for research and innovation in the Mediterranean area ( PRIMA ) program and by the Horizon 2020 ACCWA project (grant agreement # 823965 ) in the context of Marie Sklodowska-Curie Research and Innovation Staff Exchange (RISE) program. The authors wish to acknowledge the "Comunitat de Regants Canal Algerri Balaguer" and the Ebro Hydrographic Confederation (SAIH Ebro) for providing the observation irrigation data used in this study