Hypoxia inducible factor 1a in kidney cancer

https://openworks.mdanderson.org/sumexp23/1120/thumbnail.jp

Real-Time MRI-Guided Catheter Tracking Using Hyperpolarized Silicon Particles

Visualizing the movement of angiocatheters during endovascular interventions is typically accomplished using x-ray fluoroscopy. There are many potential advantages to developing magnetic resonance imaging-based approaches that will allow three-dimensional imaging of the tissue/vasculature interface while monitoring other physiologically-relevant criteria, without exposing the patient or clinician team to ionizing radiation. Here we introduce a proof-of-concept development of a magnetic resonance imaging-guided catheter tracking method that utilizes hyperpolarized silicon particles. The increased signal of the silicon particles is generated via low-temperature, solid-state dynamic nuclear polarization, and the particles retain their enhanced signal for ?40?minutes—allowing imaging experiments over extended time durations. The particles are affixed to the tip of standard medical-grade catheters and are used to track passage under set distal and temporal points in phantoms and live mouse models. With continued development, this method has the potential to supplement x-ray fluoroscopy and other MRI-guided catheter tracking methods as a zero-background, positive contrast agent that does not require ionizing radiation

NMR Spectroscopy-Based Metabolomics of Platelets to Analyze Brain Tumors

“Tumor-educated platelets” have recently generated substantial interest for the diagnosis of cancer. We hypothesized that tumor educated platelets from patients with brain tumors will reflect altered metabolism compared to platelets from healthy volunteers. Here, in a pilot study, we have employed nuclear magnetic resonance (NMR) spectroscopy in platelets from brain tumor patients to demonstrate altered metabolism compared to the platelets obtained from healthy volunteers

Additional file 1: Figure S1. of Induction of autophagy by ARHI (DIRAS3) alters fundamental metabolic pathways in ovarian cancer models

Growth of parental and ARHI-transfected SKOv3 and Hey cells. Effect of Dox treatment on the growth of parental and ARHI-transfected SKOv3 and Hey cells at 24 and 48 h. Western analysis of the effect of ARHI expression on LC3I and LC3II is also presented. Figure S2. Western analysis of GLUT1 expression following ARHI induction. Figure S3. Analysis of ARHI expression and autophagy markers during Atg5 knockdown. Effect of Atg5 knockdown on LC3I and LC3II levels during ARHI expression in SKOv3-ARHI cells. Immunofluorescence of SKOv3-ARHI cells transfected with GFP-LC3 following ARHI induction with and without Atg5 knockdown. Figure S4. Western analysis of LDH and CK expression following ARHI induction. Figure S5. Induction of ARHI expression in vivo. Expression of ARHI and LC3 in subcutaneous SKOv3-ARHI tumors at 24-72 h post-treatment with Dox. Figure S6. Expression of ACC and Phsopho-ACC by RPPA. Figure S7. Fractional 13C label incorporation from 5-13C-Gln in SKOv3-ARHI. The fractional incorporation of the glutamine 13C label into NMR-observable intracellular metabolites following induction of ARHI. (PDF 867 kb

Long noncoding RNA ceruloplasmin promotes cancer growth by altering glycolysis

Long noncoding RNAs (lncRNAs) significantly influence the development and regulation of genome expression in cells. Here, we demonstrate the role of lncRNA ceruloplasmin (NRCP) in cancer metabolism and elucidate functional effects leading to increased tumor progression. NRCP was highly upregulated in ovarian tumors, and knockdown of NRCP resulted in significantly increased apoptosis, decreased cell proliferation, and decreased glycolysis compared with control cancer cells. In an orthotopic mouse model of ovarian cancer, siNRCP delivered via a liposomal carrier significantly reduced tumor growth compared with control treatment. We identified NRCP as an intermediate binding partner between STAT1 and RNA polymerase II, leading to increased expression of downstream target genes such as glucose-6-phosphate isomerase. Collectively, we report a previously unrecognized role of the lncRNA NRCP in modulating cancer metabolism. As demonstrated, DOPC nanoparticle-incorporated siRNA-mediated silencing of this lncRNA in vivo provides therapeutic avenue toward modulating lncRNAs in cancer