Proline priming: An efficient strategy to mitigate salinity impact at early developmental stages of the oilseed halophyte Cakile maritima

Seed germination is a vital process, yet extremely sensitive to salinity. This is particularly true for coastal halophytes like the annual oilseed species Cakile maritima, which faces the simultaneous impact of wind, salt-spray and seawater inundations in its natural biotopes. At the early developmental stages, this may jeopardize seed germination, plant establishment capacity and hence its development and persistence. Osmopriming is a pre-sowing approach aiming to improve seedling emergence and establishment in adverse environments. Here, we investigate the effect of proline (at 0, 1, 5, and 20 mM) pre-treatment on salt tolerance of C. maritima at the juvenile stage under salinity (0, 100, and 200 mM NaCl). Proline seed priming enhanced the germination rate (28% to 92%) and promoted seedling establishment of C. maritima by stimulating α-amylase activity even at the highest salinity (+55 %). Besides, after transfer of non-germinated seeds on distilled water, salt impact was fully reversible. At the seedling stage, chlorophyll fluorescence parameters showed that this osmoticum increased the maximal quantum yield of PSII photochemistry (Fv/Fm) and the quantum yield of photochemical energy conversion [Y(II)]. In contrast, the quantum yield of nonregulated nonphotochemical energy dissipation [Y(NO)] and the quantum yield of regulated nonphotochemical energy dissipation [Y(NPQ)], which might be correlated to the mitigation of the salt deleterious effects on PSII. Proline and carbohydrate concentrations also increased following priming. Overall, our data provide strong arguments for using proline at low doses (1 and 5 mM) as a successful priming agent to alleviate salinity-induced adverse effect on plants

Psychological responses of Tunisian general population during COVID-19 pandemic

Introduction: this is the first study assessing the psychological impact on Tunisian general population during the first peak of the COVID-19 pandemic. We aimed to assess the prevalence of anxiety, depressive symptoms and insomnia, as well as associated factors. Methods: a cross-sectional study was conducted through an online survey of 1615 people during March 23rd to May 5th, 2020. We used the hospital anxiety and depression scale for anxiety and depression screening, and the insomnia severity index to assess sleep disturbances. Results: our study revealed a high prevalence of anxiety and depressive symptoms, and insomnia (70.9%, 71.1% and 60.6% respectively). Multivariable statistics showed that anxiety symptoms were correlated to female gender (adjusted odds ratio [aOR] 1.784, 95% confidence interval (CI 1.252-2.542; p=10ˉ3), history of mental illness (aOR: 1.680, 95% CI: 1.329-2.125; p<10ˉ3), frequently social media exposure (aOR: 1.578, 95%CI: 1.250-1.992; p<10ˉ3), times to focus on COVID-19 ≥ 3hours (aOR: 1.840, 95% CI: 1.433-2.362; p<10ˉ3), consultation with doctor in the clinic in the past 14 days (aOR: 1.821, 95%CI :1.220-2.718; p=0.003) and recent traumatic event in the past 14 days (aOR: 1.641,95% CI: 1.331-2.024; p<10ˉ3). Principal factors associated with depressive symptoms included female gender (aOR: 1.637, 95% CI: 1.150-2.331; p=0.006), history of mental illness (aOR: 1.498, 95% CI: 1.189-1.888; p=10ˉ3), times to focus on COVID-19 ≥ 3hours (aOR: 1.956, 95% CI: 1.555-2.461; p<10ˉ3), and recent traumatic event in the past 14 days (aOR: 1.558, 95% CI: 1.265-1.919; p<10ˉ3). The main factors correlated to insomnia were younger (age <35years) (aOR: 1.592, 95% CI: 1.17 -2.152; p=0.003), female gender (aOR: 1.864, 95% CI: 1.252-2.775; p=0.002), having organic diseases (aOR: 1.527, 95% CI: 1.131-2.061; p=0.006), history of mental illness (aOR: 1.777, 95% CI: 1.396-2.263; p<10ˉ3), students (aOR: 1.931, 95% CI: 1.495-2.495; p<10ˉ3), times to focus on COVID-19 ≥3hours (aOR: 1.877, 95% CI: 1.467-2.400; p<10ˉ3) and recent traumatic event (aOR: 1.431, 95% CI: 1.144-1.789; p=0.002). Conclusion: our study revealed a major mental health burden in Tunisia during COVID-19 pandemic. Many factors were correlated to anxiety, depressive symptoms and insomnia, suggesting the need for greater psychological support in general and in certain vulnerable groups

Upstream-Downstream Influence of Water Harvesting Techniques (Jessour) on Soil Water Retention in Southeast Tunisia

Weather parameters and soil moisture profiles were measured at an hourly time step during four agricultural years (September to October, from 2018–19 to 2021–22) in two Jessour (water harvesting cultivated terraces) of the same valley in Zmerten (southeastern Tunisia), characterized by an arid climate. One instrumented Jesr (singular of Jessour) was located upstream and the other one downstream. During each dry season, when crops experience water stress, the downstream Jesr had a higher available water content than the upstream one; in the downstream Jesr the soil profile moisture remained above the wilting point, whereas in the upstream soil surface, moisture levels decreased to below the wilting point. High accumulation/low intensity rains (causing saturation/excess runoff) flooded both upstream and downstream Jessour from 50 mm of cumulative rainfall, whereas high intensity/low accumulation rains (causing infiltration/excess runoff) activated the downstream Jesr from an intensity of 15.2 mm/h, and a combination of moderate intensity and moderate accumulation rains activated both Jessour from an intensity of 8 mm/h and a cumulative rainfall of 33 mm. We propose to set 50 mm of cumulative rainfall and/or 6.4 mm/h of intensity as threshold values for the activation of the Jessour system in Zmerten. However, significant soil moisture recharges can occur even without activation of the Jessour system.EEA BarilocheFil: Calianno, Martin. University of Lausanne. Institute of Geography and Sustainability and Interdisciplinary Centre for Mountain Research; SuizaFil: Calianno, Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Ben Fraj, Tarek. University of Tunis. CGMED Laboratory; TúnezFil: Ben Fraj, Tarek. University of Sousse. Faculty of Arts and Human Sciences; TúnezFil: Fallot, Jean-Michel. University of Lausanne. Institute of Geography and Sustainability and Interdisciplinary Centre for Mountain Research; SuizaFil: Abbassi, Mohamed. University of Tunis. CGMED Laboratory; TúnezFil: Abbassi, Mohamed. University of Sousse. Faculty of Arts and Human Sciences; TúnezFil: Ghram Messedi, Aziza. University of Tunis. CGMED Laboratory; TúnezFil: Ghram Messedi, Aziza. University of Sousse. Faculty of Arts and Human Sciences; TúnezFil: Ben Ouezdou, Hédi. University of Tunis. CGMED Laboratory; TúnezFil: Ben Ouezdou, Hédi. University of Sousse. Faculty of Arts and Human Sciences; TúnezFil: Reynard, Emmanuel. University of Lausanne. Institute of Geography and Sustainability and Interdisciplinary Centre for Mountain Research; Suiz

Benefits of Water-Harvesting Systems (Jessour) on Soil Water Retention in Southeast Tunisia

In this paper, we quantify the water balance of Jessour at the scale of agricultural plots. Jessour (plural of Jesr) are ancestral hydro-agricultural systems in the Dahar plateau (southeastern Tunisia). They consist of small dams built across wadis and gullies, which retain rainwater and sediments, hence enabling cropping. Despite arid climate conditions, Jessour allow the culture of the olive tree beyond its ecological limits. Weather monitoring stations were set up and soil moisture sensors installed down to a depth of 1.25 m in the soil in two neighboring gullies in the village of Zammour: one with a Jesr and one without. Laser granulometry and organic matter analyses were carried out on samples collected near the soil moisture sensors. Measurements were recorded from 28 September 2017 to 21 September 2018. From 10 to 12 November 2017, the region received 123.3 mm rainfall. The Jesr retained the equivalent of 410.3 mm of soil moisture to a depth of 1.25 m whereas the value in the gully was 224.6 mm. Throughout the summer of 2018, the soil available water capacity (AWC) remained above 55 mm in the Jesr, while it dropped to zero in the gully. Jessour are thus very suitable hydro-agricultural systems to face the climate changes concerning this fragile region, located in the transition zone between the semi-arid to arid Mediterranean region and the Sahara

Subclinical atherosclerosis in Behcet's disease: A systematic review and meta-analysis.

OBJECTIVE: To evaluate subclinical atherosclerosis in Behcet disease (BD), we performed a systematic review and meta-analysis of studies where atherosclerosis was determined by flow-mediated dilatation (FMD) and endothelial-mediated dilatation (EMD) and by measurement of intima media thickness (IMT) of carotid arteries. METHODS: Systematic search of EMBASE and PubMed databases from January 2000 to January 2014 according to PRISMA guidelines. RESULTS: Nine studies met the inclusion criteria on FMD/EMD, 11 on IMT and 4 on both. BD had lower FMD than controls (SMD = -0.89, 95% CI: -0.660 to -1.11, p < 0.001), which was confirmed by subgroup analyses on active and inactive patients (SMD = -1.17, 95% CI: -1.45 to -0.89 and SMD = -0.72, 95% CI: -0.97 to -0.46, p = 0.0001 for both). EMD was lower in BD but with a large estimate (SMD = 0.38, 95% CI: -0.79 to -0.03, p = 0.06, I(2) = 82.2%). IMT was greater in BD and the large estimate (SMD = 0.95, 95% CI: 0.63-1.28, p < 0.0001, I(2) = 87.6%) persisted after subgroup analysis on active and inactive patients (I(2) = 88.4% and 86.7%, respectively). Pooling IMT studies by a Newcastle Ottawa Scale of 5 and 6/7 yielded lower estimates (SMD = 0.54, 95% CI: 0.32-0.75, p < 0.0001, I(2) = 58.7% and SMD = 1.72, 95% CI: 1.35-2.09 p < 0.05, I(2) = 48.6%). CONCLUSIONS: FMD is impaired in BD even in inactive state and IMT is greater despite a degree of statistical heterogeneity that reflects the clinical heterogeneity of BD. Future prospective studies should account for risk stratification of atherosclerosis in BD

Genetic Association of a Gain-of-Function IFNGR1 Polymorphism and the Intergenic Region LNCAROD/DKK1 With Behcet's Disease

Objective. Behçet’s disease is a complex systemic inflammatory vasculitis of incompletely understood etiology. This study was undertaken to investigate genetic associations with Behçet’s disease in a diverse multiethnic population.Methods. A total of 9,444 patients and controls from 7 different populations were included in this study. Genotyping was performed using an Infinium ImmunoArray- 24 v.1.0 or v.2.0 BeadChip. Analysis of expression data from stimulated monocytes, and epigenetic and chromatin interaction analyses were performed.Results. We identified 2 novel genetic susceptibility loci for Behçet’s disease, including a risk locus in IFNGR1(rs4896243) (odds ratio [OR] 1.25; P = 2.42 × 10−9) and within the intergenic region LNCAROD/DKK1 (rs1660760) (OR 0.78; P = 2.75 × 10−8). The risk variants in IFNGR1 significantly increased IFNGR1 messenger RNA expression in lipopolysaccharide- stimulated monocytes. In addition, our results replicated the association (P 30 genetic susceptibility loci with a suggestive level of association (P < 5 × 10−5), which will require replication. Finally, functional annotation of genetic susceptibility loci in Behçet’s disease revealed their possible regulatory roles and suggested potential causal genes and molecular mechanisms that could be further investigated.Conclusion. We performed the largest genetic association study in Behçet’s disease to date. Our findings reveal novel putative functional variants associated with the disease and replicate and extend the genetic associations in other loci across multiple ancestries

Effects of non-uniform root zone salinity on water use, Na+ recirculation, and Na+ and H+ flux in cotton

A new split-root system was established through grafting to study cotton response to non-uniform salinity. Each root half was treated with either uniform (100/100 mM) or non-uniform NaCl concentrations (0/200 and 50/150 mM). In contrast to uniform control, non-uniform salinity treatment improved plant growth and water use, with more water absorbed from the non- and low salinity side. Non-uniform treatments decreased Na+ concentrations in leaves. The [Na+] in the ‘0’ side roots of the 0/200 treatment was significantly higher than that in either side of the 0/0 control, but greatly decreased when the ‘0’ side phloem was girdled, suggesting that the increased [Na+] in the ‘0’ side roots was possibly due to transportation of foliar Na+ to roots through phloem. Plants under non-uniform salinity extruded more Na+ from the root than those under uniform salinity. Root Na+ efflux in the low salinity side was greatly enhanced by the higher salinity side. NaCl-induced Na+ efflux and H+ influx were inhibited by amiloride and sodium orthovanadate, suggesting that root Na+ extrusion was probably due to active Na+/H+ antiport across the plasma membrane. Improved plant growth under non-uniform salinity was thus attributed to increased water use, reduced leaf Na+ concentration, transport of excessive foliar Na+ to the low salinity side, and enhanced Na+ efflux from the low salinity root

Impact of Systemic Inflammation and Autoimmune Diseases on apoA-I and HDL Plasma Levels and Functions

The cholesterol of high-density lipoproteins (HDLs) and its major proteic component, apoA-I, have been widely investigated as potential predictors of acute cardiovascular (CV) events. In particular, HDL cholesterol levels were shown to be inversely and independently associated with the risk of acute CV diseases in different patient populations, including autoimmune and chronic inflammatory disorders. Some relevant and direct anti-inflammatory activities of HDL have been also recently identified targeting both immune and vascular cell subsets. These studies recently highlighted the improvement of HDL function (instead of circulating levels) as a promising treatment strategy to reduce inflammation and associated CV risk in several diseases, such as systemic lupus erythematosus and rheumatoid arthritis. In these diseases, anti-inflammatory treatments targeting HDL function might improve both disease activity and CV risk. In this narrative review, we will focus on the pathophysiological relevance of HDL and apoA-I levels/functions in different acute and chronic inflammatory pathophysiological conditions

Automatisation d'un spectrometre de masse : application a l'analyse des gaz residuels d'une capsule contenant une puce AsGa

SIGLECNRS T Bordereau / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc