Modifications of Graphene Prepared by Chemical Vapor Deposition for Diagnostic Applications

xx, 186 p.El grafeno es un alótropo de carbono que ha demostrado tener excelentes propiedades electrónicas, mecánicas y térmicas. Gracias a ello, tiene un gran potencial en campos como biomedicina o electrónica. Entre sus distintos derivados, el grafeno crecido por deposición química de vapor (CVD) y transferido sobre superficie, ha demostrado ser un material idóneo para este tipo de aplicaciones debido a su elevada conductividad, carácter ambipolar de efecto campo, y una relación calidad/coste de producción conveniente. Sin embargo, la investigación del grafeno aún está en sus inicios y es necesaria una alta reproducibilidad de sus resultados para la futura comercialización y estandarización de los dispositivos de grafeno. Para ello, en esta tesis se ha desarrollado un protocolo de limpieza posterior al proceso de litografía con el fin de eliminar los residuos poliméricos de dicho proceso y obtener el mejor rendimiento electrónico. Además, la implementación de capacidades sensóricas sobre dispositivos electrónicos como los transistores de grafeno permite el desarrollo de herramientas para diagnóstico y tratamiento de diversas condiciones neurológicas como epilepsia o Parkinson. Con este propósito, el grafeno ha sido modificado covalentemente vía una adición radicalaria siguiendo diferentes estrategias compatibles con el diseño del dispositivo, con el fin de anclar los bio-receptores de interés. Como prueba de concepto, el sistema descrito se usó con un aptámero selectivo para trombina, demostrando resultados prometedores.Así mismo, se demostró por primera vez el uso del grafeno CVD en espectrometría de masas MALDI-TOF gracias a su capacidad de ionización/desorción. En concreto, se ha desarrollado un sistema compuesto por diferentes azucares modificados sobre grafeno como herramienta diagnóstica para la detección de proteínas. En este dispositivo se usó el grafeno como superficie asistente para la ionización/desorción láser en espectrometría de masas que, combinado al carácter conductor del grafeno, ha permitido reemplazar ITO como material de soporte y trabajar en condiciones de ausencia de matriz

Using machine learning methods to determine a typology of patients with HIV-HCV infection to be treated with antivirals

Several European countries have established criteria for prioritising initiation of treatment in patients infected with the hepatitis C virus (HCV) by grouping patients according to clinical characteristics. Based on neural network techniques, our objective was to identify those factors for HIV/HCV co-infected patients (to which clinicians have given careful consideration before treatment uptake) that have not being included among the prioritisation criteria. This study was based on the Spanish HERACLES cohort (NCT02511496) (April-September 2015, 2940 patients) and involved application of different neural network models with different basis functions (product-unit, sigmoid unit and radial basis function neural networks) for automatic classification of patients for treatment. An evolutionary algorithm was used to determine the architecture and estimate the coefficients of the model. This machine learning methodology found that radial basis neural networks provided a very simple model in terms of the number of patient characteristics to be considered by the classifier (in this case, six), returning a good overall classification accuracy of 0.767 and a minimum sensitivity (for the classification of the minority class, untreated patients) of 0.550. Finally, the area under the ROC curve was 0.802, which proved to be exceptional. The parsimony of the model makes it especially attractive, using just eight connections. The independent variable "recent PWID" is compulsory due to its importance. The simplicity of the model means that it is possible to analyse the relationship between patient characteristics and the probability of belonging to the treated group

Information and digital technologies of Industry 4.0 and Lean supply chain management: a systematic literature review

The purpose of this paper is to provide an overview of the current state of research and the key aspects and implications of the relationships between Information and Digital Technologies (IDT) of Industry 4.0 and Lean Supply Chain Management (LSCM), with the identification of the lines of research developed and an analysis of the main findings. A Systematic Literature Review methodology has been used for the identification, selection, and evaluation of the published research. A set of 78 papers deduced from the most relevant scientific databases, including Web of Science, Scopus, and ABI/Inform, from 1996 to December 2019, has been analyzed and synthesized. The analysis and evaluation of these papers has enabled a new classification of the literature to be offered that identifies four lines of research based on the Life Cycle of Technology: obsolete IDT in LSCM; mature IDT in LSCM; emerging IDT in LSCM; and an Information Systems and IDT general approach in LSCM. The paper goes on to discuss the gaps found in the literature and proposes new opportunities and challenges for future research. A series of implications are presented intended to be useful from not only an academic point-of-view but also from a management focus, including recommendations for industrial managers and policymakers

Experience with the use of Rituximab for the treatment of rheumatoid arthritis in a tertiary Hospital in Spain: RITAR study

There is evidence supporting that there are no relevant clinical differences between dosing rituximab 1000 mg or 2000 mg per cycle in rheumatoid arthritis (RA) patients in clinical trials, and low-dose cycles seem to have a better safety profile. Our objective was to describe the pattern of use of rituximab in real-life practice conditions. Methods: Rituximab for RA in clinical practice (RITAR) study is a retrospective cohort study from 2005 to 2015. Eligibility criteria were RA adults treated with rituximab for active articular disease. Response duration was the main outcome defined as months elapsed from the date of rituximab first infusion to the date of flare. A multivariable analysis was performed to determine the variables associated with response duration. Results: A total of 114 patients and 409 cycles were described, 93.0% seropositive and 80.7% women. Rituximab was mainly used as second-line biological therapy. On demand retreatment was used in 94.6% of cases versus fixed 6 months retreatment in 5.4%. Median response duration to on demand rituximab cycles was 10 months (interquartile range, 7–13). Multivariable analysis showed that age older than 65 years, number of rituximab cycles, seropositivity, and first- or second-line therapy were associated with longer response duration. The dose administered at each cycle was not significantly associated with response duration. Conclusions: Our experience suggests that 1000 mg rituximab single infusion on demand is a reasonable schedule for long-term treatment of those patients with good response after the first cycles, especially in seropositive patients and when it is applied as a first- or second-line biological therap

Forecasting of poor visibility episodes in the vicinity of Tenerife Norte Airport

Aviation safety is a priority that may be compromised by adverse weather conditions. This is the case for poor visibility in the vicinity of airports, which can pose a risk during takeoff and landing. For airports that are prone to fog because of their location, an accurate forecast of poor-visibility episodes is vital. However, the forecasting of low clouds is still a challenge in numerical weather prediction, especially when an airport is near complex terrain for which the use of non-hydrostatic mesoscale models is mandatory. All these factors are present at Tenerife Norte Airport, which is commonly affected by poor visibility from low clouds related to persistent trade winds and moist flows from the Atlantic Ocean. In this paper, several methods for estimating visibility based on mesoscale model outputs are tested. Use of the HARMONIE-AROME model is encouraged because of its excellent performance in the detection of poor-visibility episodes (False Alarm Ratio = 0.34–0.38; Frequency Of Misses = 0.22–0.38, depending on the model version and method used). In addition, the use of satellite application facilities is proposed for the nowcasting of low clouds affecting the airport area. Specifically, we used products that estimate cloud type, cloud top altitude, and integrated water vapor content in the boundary layer. Finally, an application is presented for the monitoring of weather conditions in real time to estimate poor-visibility risk.This work was supported by the Aeronautics Annual Plan 2018 of AEMET

The animal model determines the results on Aeromonas virulence factors.

The selection of an experimental animal model is of great importance in the study of bacterial virulence factors. Here, a bath infection of zebrafish larvae is proposed as an alternative model to study the virulence factors of Aeromonas hydrophila. Intraperitoneal infections in mice and trout were compared with bath infections in zebrafish larvae using specific mutants. The great advantage of this model is that bath immersion mimics the natural route of infection, and injury to the tail also provides a natural portal of entry for the bacteria. The implication of T3SS in the virulence of A. hydrophila was analyzed using the AH-1::aopB mutant. This mutant was less virulent than the wild-type strain when inoculated into zebrafish larvae, as described in other vertebrates. However, the zebrafish model exhibited slight differences in mortality kinetics only observed using invertebrate models. Infections using the mutant AH-11vapA lacking the gene coding for the surface S-layer suggested that this protein was not totally necessary to the bacteria once it was inside the host, but it contributed to the inflammatory response. Only when healthy zebrafish larvae were infected did the mutant produce less mortality than the wild-type. Variations between models were evidenced using the AH-11rmlB, which lacks the O-antigen lipopolysaccharide (LPS), and the AH-11wahD, which lacks the O-antigen LPS and part of the LPS outer-core. Both mutants showed decreased mortality in all of the animal models, but the differences between them were only observed in injured zebrafish larvae, suggesting that residues from the LPS outer core must be important for virulence. The greatest differences were observed using the AH- 11FlaB-J (lacking polar flagella and unable to swim) and the AH-1::motX (non-motile but producing flagella). They were as pathogenic as the wild-type strain when injected into mice and trout, but no mortalities were registered in zebrafish larvae. This study demonstrates that zebrafish larvae can be used as a host model to assess the virulence factors of A. hydrophila. This model revealed more differences in pathogenicity than the in vitro models and enabled the detection of slight variations in pathogenesis not observed using intraperitoneal injections of mice or fish

Comisión de plan de estudios de la titulación de Ingeniería Técnica de Telecomunicaciones, especialidad en Sonido e Imagen de la EPS

La red docente de la Comisión de plan de estudios de la titulación de Ingeniería Técnica de Telecomunicación, especialidad en Sonido e Imagen de la EPS ha realizado durante el curso 2007/08 un estudio de los objetivos y competencias del futuro título de grado, así como el análisis y diseño de la posible estructura en bloques y asignaturas obligatorias en la que se podría distribuir dicho título. El estudio toma como base los resultados obtenidos en redes de cursos anteriores (ver memoria de redes 2005/06 y 2006/07), las cuales estaban orientadas al diseño curricular dentro del marco de los créditos ECTS para la convergencia al Espacio Europeo de Educación Superior, y sobre todo, se basa en la experiencia de los propios participantes en trabajos o redes previas. El objetivo principal de este proyecto es el diseño curricular del futuro título de grado en Ingeniería de Telecomunicación en Sonido e Imagen, directamente relacionada con la actual Ingeniería Técnica de Telecomunicación, especialidad en Sonido e Imagen, que se imparte en la Universidad de Alicante. Para ello se han seguido las pautas generales establecidas por el Real Decreto de ordenación de Enseñanzas Universitarias Oficiales (BOE, 30 de octubre de 2007), así como otros documentos elaborados por el Colegio Oficial de Ingenieros Técnicos de Telecomunicación y la Comisión de Universidades de Ingeniería Técnica de Telecomunicación

Thyroid hormones inhibit TGF-β signaling and attenuate fibrotic responses

TGF-β, the most potent profibrogenic factor, acts by activating SMAD (mothers against decapentaplegic) transcription factors, which bind to SMAD-binding elements in target genes. Here, we show that the thyroid hormone triiodothyronine (T3), through binding to its nuclear receptors (TRs), is able to antagonize transcriptional activation by TGF-β/SMAD. This antagonism involves reduced phosphorylation of SMADs and a direct interaction of the receptors with SMAD3 and SMAD4 that is independent of T3-mediated transcriptional activity but requires residues in the receptor DNA binding domain. T3 reduces occupancy of SMADbinding elements in response to TGF-β, reducing histone acetylation and inhibiting transcription. In agreement with this transcriptional cross-talk, T3 is able to antagonize fibrotic processes in vivo. Liver fibrosis induced by carbon tetrachloride is attenuated by thyroid hormone administration to mice, whereas aged TR knockout mice spontaneously accumulate collagen. Furthermore, skin fibrosis induced by bleomycin administration is also reduced by the thyroid hormones. These findings define an important function of the thyroid hormone receptors and suggest TR ligands could have beneficial effects to block the progression of fibrotic diseases.This work was supported by Grants BFU2011-28058 and BFU2014-53610P from Ministerio de Economía y Competitividad; S2011/BMD-2328 TIRONET from the Comunidad de Madrid; and RD12/0036/0030 from the Instituto de Salud Carlos III. The cost of this publication has been paid in part by FEDER fund

Design and test of Geophonino-3D: A low-cost three-component seismic noise recorder for the application of the H/V method

Earthquake effects are strongly related with the properties of the local geology, amplifying the ground motion, especially in soft soils. This amplification is connected with the resonant frequency, which can be estimated applying the horizontal-to-vertical spectral ratio (H/V) method on seismic noise measurements. In this work, a low-cost user-friendly data acquisition system (Geophonino-3D) has been designed for three-component seismic noise recording. The developed system consists of the signal conditioning circuit and the Arduino Due with an SD Shield for data storage. The suitability of the equipment for the application of the H/V method has been first assessed in the laboratory, according to the experimental sensitivity, the internal noise stability and the channel consistency. Subsequently, experimental verification has been carried out by recording seismic noise at eight different sites along the province of Alicante (southeast Spain), with different soil characteristics. The performance of the developed acquisition system has been evaluated in terms of the power spectral density and the H/V peaks by comparison with the results obtained through a commercial seismic recorder. The obtained results show the applicability of the developed acquisition system to record seismic noise and to apply the H/V method. Geophonino-3D is an open source and open hardware system and the price of its components is much lower than any other commercial equipment, which becomes a significant advance especially for small research groups with reduced economic support.This research has been supported by the Conselleria d’Educació, Investigació, Cultura i Sport de la Generalitat Valenciana (project AICO/2016/098)