Ventricular volume expansion in presymptomatic genetic frontotemporal dementia

Objective: To characterize the time course of ventricular volume expansion in genetic frontotemporal dementia (FTD) and identify the onset time and rates of ventricular expansion in presymptomatic FTD mutation carriers. Methods: Participants included patients with a mutation in MAPT, PGRN, or C9orf72, or first-degree relatives of mutation carriers from the GENFI study with MRI scans at study baseline and at 1 year follow-up. Ventricular volumes were obtained from MRI scans using FreeSurfer, with manual editing of segmentation and comparison to fully automated segmentation to establish reliability. Linear mixed models were used to identify differences in ventricular volume and in expansion rates as a function of time to expected disease onset between presymptomatic carriers and noncarriers. Results: A total of 123 participants met the inclusion criteria and were included in the analysis (18 symptomatic carriers, 46 presymptomatic mutation carriers, and 56 noncarriers). Ventricular volume differences were observed 4 years prior to symptom disease onset for presymptomatic carriers compared to noncarriers. Annualized rates of ventricular volume expansion were greater in presymptomatic carriers relative to noncarriers. Importantly, time-intensive manually edited and fully automated ventricular volume resulted in similar findings. Conclusions: Ventricular volume differences are detectable in presymptomatic genetic FTD. Concordance of results from time-intensive manual editing and fully automatic segmentation approaches support its value as a measure of disease onset and progression in future studies in both presymptomatic and symptomatic genetic FTD

Modeling and optimization of sensory changes and shelf-life in vacuum-packaged cooked ham treated by E-beam irradiation

[EN] The E-beam irradiation of vacuum-packaged RTE cooked ham was carried out to establish the dose required to achieve the food safety objective (FSO) and to minimize changes in selected sensory attributes. Cooked ham was irradiated with doses ranging 1-4 kGy. After the treatment, the microbial inactivation of Listeria monocytogenes, the shelf-life of the product and some sensory attributes (appearance, odor, and flavor) were determined. The inactivation of L monocytogenes was satisfactorily described by a first-order kinetics equation (R2=0.99). The influence of the irradiation dose on appearance, odor, and flavor was modeled through Gompertz (R2=0.99, for appearance) and Activation/Inactivation (R2=0.99, for odor and flavor) equations. A model was also developed to determine the shelf-life of irradiated cooked ham depending on the irradiation dose (R2 > 0.91). The dose that maximized the scores of the sensory attributes was 0.96 kGy resulting in an acceptable sensory quality for 80 days. It is possible to apply up to 2 kGy to ensure microbial safety, while provoking no significant changes in the above mentioned sensory attributes. (C) 2010 Elsevier Ltd. All rights reserved.The authors acknowledge the financial support from the Project CSD2007-00016 (CONSOLIDER-INGENIO 2010) funded by the Spanish Ministry of Science and Innovation.Benedito Fort, JJ.; Cambero, MI.; Ortuño Cases, C.; Cabeza, MC.; Ordoñez, JA.; De La Hoz, L. (2011). Modeling and optimization of sensory changes and shelf-life in vacuum-packaged cooked ham treated by E-beam irradiation. Radiation Physics and Chemistry. 80(3):505-513. https://doi.org/10.1016/j.radphyschem.2010.11.001S50551380

Neuronal pentraxin 2 : a synapse-derived CSF biomarker in genetic frontotemporal dementia

Introduction: Synapse dysfunction is emerging as an early pathological event in frontotemporal dementia (FTD), however biomarkers are lacking. We aimed to investigate the value of cerebrospinal fluid (CSF) neuronal pentraxins (NPTXs), a family of proteins involved in homeostatic synapse plasticity, as novel biomarkers in genetic FTD. Methods: We included 106 presymptomatic and 54 symptomatic carriers of a pathogenic mutation in GRN, C9orf72 or MAPT, and 70 healthy non-carriers participating in the Genetic Frontotemporal dementia Initiative (GENFI), all of whom had at least one CSF sample. We measured CSF concentrations of NPTX2 using an in-house ELISA, and NPTX1 and NPTX receptor (NPTXR) by Western blot. We correlated NPTX2 with corresponding clinical and neuroimaging datasets as well as with CSF neurofilament light chain (NfL) using linear regression analyses. Results: Symptomatic mutation carriers had lower NPTX2 concentrations (median 643 pg/mL, IQR (301-872)) than presymptomatic carriers (1003 pg/mL (624-1358), p<0.001) and non-carriers (990 pg/mL (597-1373), p<0.001) (corrected for age). Similar results were found for NPTX1 and NPTXR. Among mutation carriers, NPTX2 concentration correlated with several clinical disease severity measures, NfL and grey matter volume of the frontal, temporal and parietal lobes, insula and whole brain. NPTX2 predicted subsequent decline in phonemic verbal fluency and Clinical Dementia Rating scale plus FTD modules. In longitudinal CSF samples, available in 13 subjects, NPTX2 decreased around symptom onset and in the symptomatic stage. Discussion: We conclude that NPTX2 is a promising synapse-derived disease progression biomarker in genetic FTD

Plasma glial fibrillary acidic protein is raised in progranulin-associated frontotemporal dementia

Background There are few validated fluid biomarkers in frontotemporal dementia (FTD). Glial fibrillary acidic protein (GFAP) is a measure of astrogliosis, a known pathological process of FTD, but has yet to be explored as potential biomarker. Methods Plasma GFAP and neurofilament light chain (NfL) concentration were measured in 469 individuals enrolled in the Genetic FTD Initiative: 114 C9orf72 expansion carriers (74 presymptomatic, 40 symptomatic), 119 GRN mutation carriers (88 presymptomatic, 31 symptomatic), 53 MAPT mutation carriers (34 presymptomatic, 19 symptomatic) and 183 non-carrier controls. Biomarker measures were compared between groups using linear regression models adjusted for age and sex with family membership included as random effect. Participants underwent standardised clinical assessments including the Mini-Mental State Examination (MMSE), Frontotemporal Lobar Degeneration-C linical Dementia Rating scale and MRI. Spearman's correlation coefficient was used to investigate the relationship of plasma GFAP to clinical and imaging measures. Results Plasma GFAP concentration was significantly increased in symptomatic GRN mutation carriers (adjusted mean difference from controls 192.3 pg/mL, 95% CI 126.5 to 445.6), but not in those with C9orf72 expansions (9.0, -61.3 to 54.6), MAPT mutations (12.7, -33.3 to 90.4) or the presymptomatic groups. GFAP concentration was significantly positively correlated with age in both controls and the majority of the disease groups, as well as with NfL concentration. In the presymptomatic period, higher GFAP concentrations were correlated with a lower cognitive score (MMSE) and lower brain volume, while in the symptomatic period, higher concentrations were associated with faster rates of atrophy in the temporal lobe. Conclusions Raised GFAP concentrations appear to be unique to GRN-related FTD, with levels potentially increasing just prior to symptom onset, suggesting that GFAP may be an important marker of proximity to onset, and helpful for forthcoming therapeutic prevention trials

Age at symptom onset and death and disease duration in genetic frontotemporal dementia : an international retrospective cohort study

Background: Frontotemporal dementia is a heterogenous neurodegenerative disorder, with about a third of cases being genetic. Most of this genetic component is accounted for by mutations in GRN, MAPT, and C9orf72. In this study, we aimed to complement previous phenotypic studies by doing an international study of age at symptom onset, age at death, and disease duration in individuals with mutations in GRN, MAPT, and C9orf72. Methods: In this international, retrospective cohort study, we collected data on age at symptom onset, age at death, and disease duration for patients with pathogenic mutations in the GRN and MAPT genes and pathological expansions in the C9orf72 gene through the Frontotemporal Dementia Prevention Initiative and from published papers. We used mixed effects models to explore differences in age at onset, age at death, and disease duration between genetic groups and individual mutations. We also assessed correlations between the age at onset and at death of each individual and the age at onset and at death of their parents and the mean age at onset and at death of their family members. Lastly, we used mixed effects models to investigate the extent to which variability in age at onset and at death could be accounted for by family membership and the specific mutation carried. Findings: Data were available from 3403 individuals from 1492 families: 1433 with C9orf72 expansions (755 families), 1179 with GRN mutations (483 families, 130 different mutations), and 791 with MAPT mutations (254 families, 67 different mutations). Mean age at symptom onset and at death was 49\ub75 years (SD 10\ub70; onset) and 58\ub75 years (11\ub73; death) in the MAPT group, 58\ub72 years (9\ub78; onset) and 65\ub73 years (10\ub79; death) in the C9orf72 group, and 61\ub73 years (8\ub78; onset) and 68\ub78 years (9\ub77; death) in the GRN group. Mean disease duration was 6\ub74 years (SD 4\ub79) in the C9orf72 group, 7\ub71 years (3\ub79) in the GRN group, and 9\ub73 years (6\ub74) in the MAPT group. Individual age at onset and at death was significantly correlated with both parental age at onset and at death and with mean family age at onset and at death in all three groups, with a stronger correlation observed in the MAPT group (r=0\ub745 between individual and parental age at onset, r=0\ub763 between individual and mean family age at onset, r=0\ub758 between individual and parental age at death, and r=0\ub769 between individual and mean family age at death) than in either the C9orf72 group (r=0\ub732 individual and parental age at onset, r=0\ub736 individual and mean family age at onset, r=0\ub738 individual and parental age at death, and r=0\ub740 individual and mean family age at death) or the GRN group (r=0\ub722 individual and parental age at onset, r=0\ub718 individual and mean family age at onset, r=0\ub722 individual and parental age at death, and r=0\ub732 individual and mean family age at death). Modelling showed that the variability in age at onset and at death in the MAPT group was explained partly by the specific mutation (48%, 95% CI 35\u201362, for age at onset; 61%, 47\u201373, for age at death), and even more by family membership (66%, 56\u201375, for age at onset; 74%, 65\u201382, for age at death). In the GRN group, only 2% (0\u201310) of the variability of age at onset and 9% (3\u201321) of that of age of death was explained by the specific mutation, whereas 14% (9\u201322) of the variability of age at onset and 20% (12\u201330) of that of age at death was explained by family membership. In the C9orf72 group, family membership explained 17% (11\u201326) of the variability of age at onset and 19% (12\u201329) of that of age at death. Interpretation: Our study showed that age at symptom onset and at death of people with genetic frontotemporal dementia is influenced by genetic group and, particularly for MAPT mutations, by the specific mutation carried and by family membership. Although estimation of age at onset will be an important factor in future pre-symptomatic therapeutic trials for all three genetic groups, our study suggests that data from other members of the family will be particularly helpful only for individuals with MAPT mutations. Further work in identifying both genetic and environmental factors that modify phenotype in all groups will be important to improve such estimates. Funding: UK Medical Research Council, National Institute for Health Research, and Alzheimer's Society

Natural clusters of tuberous sclerosis complex (TSC)-associated neuropsychiatric disorders (TAND): new findings from the TOSCA TAND research project.

BACKGROUND: Tuberous sclerosis complex (TSC)-associated neuropsychiatric disorders (TAND) have unique, individual patterns that pose significant challenges for diagnosis, psycho-education, and intervention planning. A recent study suggested that it may be feasible to use TAND Checklist data and data-driven methods to generate natural TAND clusters. However, the study had a small sample size and data from only two countries. Here, we investigated the replicability of identifying natural TAND clusters from a larger and more diverse sample from the TOSCA study. METHODS: As part of the TOSCA international TSC registry study, this embedded research project collected TAND Checklist data from individuals with TSC. Correlation coefficients were calculated for TAND variables to generate a correlation matrix. Hierarchical cluster and factor analysis methods were used for data reduction and identification of natural TAND clusters. RESULTS: A total of 85 individuals with TSC (female:male, 40:45) from 7 countries were enrolled. Cluster analysis grouped the TAND variables into 6 clusters: a scholastic cluster (reading, writing, spelling, mathematics, visuo-spatial difficulties, disorientation), a hyperactive/impulsive cluster (hyperactivity, impulsivity, self-injurious behavior), a mood/anxiety cluster (anxiety, depressed mood, sleep difficulties, shyness), a neuropsychological cluster (attention/concentration difficulties, memory, attention, dual/multi-tasking, executive skills deficits), a dysregulated behavior cluster (mood swings, aggressive outbursts, temper tantrums), and an autism spectrum disorder (ASD)-like cluster (delayed language, poor eye contact, repetitive behaviors, unusual use of language, inflexibility, difficulties associated with eating). The natural clusters mapped reasonably well onto the six-factor solution generated. Comparison between cluster and factor solutions from this study and the earlier feasibility study showed significant similarity, particularly in cluster solutions. CONCLUSIONS: Results from this TOSCA research project in an independent international data set showed that the combination of cluster analysis and factor analysis may be able to identify clinically meaningful natural TAND clusters. Findings were remarkably similar to those identified in the earlier feasibility study, supporting the potential robustness of these natural TAND clusters. Further steps should include examination of larger samples, investigation of internal consistency, and evaluation of the robustness of the proposed natural clusters

The Project Ptdc/cte-gix/098968/2008 - High Resolution Stratigraphy Of The Lower Jurassic Organic-rich Marine Series In The Lusitanian Basin (portugal): Initial Expectations And Results [o Projecto Ptdc/cte-gix/098968/2008 - Estratigrafia De Alta Resolução Das Séries Marinhas Ricas Em Matéria Orgânica Do Jurássico Inferior Da Bacia Lusitânica: Perspectivas Iniciais E Resultados Obtidos]

This work presents the main results of the Project "High resolution stratigraphy of the Lower Jurassic organic-rich marine series in the Lusitanian Basin (Portugal)", supported by the Portuguese Foundation for Science and Technology. This project was focused on a multidisciplinary stratigraphic analysis based on the study of a range of biotic and abiotic parameters of the Lower Jurassic marl-limestone succession rich in organic matter, embracing seven main objectives: 1. Sedimentological (mineralogical, textural and compositional) and sequential analysis of the whole Lower Jurassic hemipelagic succession (Upper Sinemurian-Toarcian); 2. Ammonite biostratigraphic precisions of the Upper Sinemurian and Upper Pliensbachian; 3. Palaeontological and palaeoecological analysis of the benthic macrofauna (especially brachiopods and bivalves); 4. Biostratigraphical, taxonomic and palaeoecological analysis of the ostracod fauna (Sinemurian-Toarcian); 5. Chemostratigraphic analysis based mainly on carbon stable isotopes (Sinemurian-Pliensbachian); 6. Organic geochemical and petrographic analysis (total organic carbon, palynofacies, Rock-Eval pyrolysis and biomarkers) of the Sinemurian-Pliensbachian; 7. Vertical variation patterns of gamma-ray analysis (Sinemurian- Toarcian). Besides the new results in each specific scientific domain (for example, biostratigraphy, palaeoecology, sedimentology, sequence stratigraphy and cyclicity, chemostratigraphy and organic geochemistry), the integration of all data, in several cases at highresolution level, allows emphasizing the achievement of two main transverse goals: the improvement of the stratigraphy and sedimentary knowledge of the Lower Jurassic series of western Iberia margin, with implications in the palaoenvironmental and palaeoceanographic interpretations of the Tethyan pre-Atlantic basins dynamics and global correlation; taking into consideration the huge number of organic geochemical parameters analyzed, the results of this research contributed significantly to understand the role of the different Sinemurian and Pliensbachian organic-rich intervals as potential hydrocarbon generation sources (quality of organic matter and maturation). © 2013 LNEG - Laboratório Nacional de Geologia e Energia IP.100SPEC.ISS.1713Azerêdo, A.C., Duarte, L.V., Henriques, M.H., Manuppella, G., Da dinâmica continental no Triásico aos mares do Jurássico Inferior e Médio (2003) Cadernos de Geologia de Portugal, p. 43. , Instituto Geológico e MineiroAzerêdo, A.C., Mendonça Filho, J.G., Cabral, M.C., Duarte, L.V., Calcários pedogénicos e níveis ricos em matéria orgânica no Jurássico Médio da Pedreira do Galinha, Serra de Aire: Abordagem multi-disciplinar (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 95-100. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosBarrón, E., Comas-Rengifo, M.J., Duarte, L.V., Palynomorph succession of the Upper Pliensbachian-Lower Toarcian of the Peniche section (Portugal) (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 55-61. , In: Duarte, L.V., Silva, R.L., Azerêdo (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosBarroso-Barcenilla, F., Comas-Rengifo, M.J., Duarte, L.V., Goy, A., Martínez, G., A new genus of nautiloid in the Toarcian of the Iberian Peninsula (Spain and Portugal) (2011) Bulletin de la Société Géologique de France, 182 (5), pp. 391-404Boussaha, M., Caractérisation spatiale des paléoenvironnements au Sinémurien (Bassin Lusitanien) sur la base des assemblages à nannofossiles calcaires (2011) Master Sciences Technologies Santé mention Sciences de la Terre spécialité Paléontologie Sédimentologie Paléoenvironnements, 1, p. 49. , Université Claude Bernard LyonCabral, M.C., Azerêdo, A.C., Colin, J.-P., Silva, R.L., Duarte, L.V., Associações de ostracodos da Formação de Coimbra (Sinemuriano) de S. Pedro de Moel: Valor paleoecológico e paleobiogeográfico (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 43-47. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosCabral, M.C., Lord, A.R., Boomer, I.D., Loureiro, I.M., Malz, H., Tanycythere gen. nov. and its significance for Jurassic ostracod diversity patterns Palaeontology, , (submetido)Cabral, M.C., Loureiro, I.M., Duarte, L.V., Azerêdo, A.C., Registo da extinção dos Metacopina (Ostracoda, Crustacea) no Toarciano de Rabaçal, região de Coimbra (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 63-68. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosComas-Rengifo, M.J., Duarte, L.V., García Joral, F., Goy, A., Los braquiópodos del Toarciense inferior (Jurásico) en el área de Rabaçal-Condeixa (Portugal): Distribución estratigráfica y paleobiogeografía (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 37-42. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosComas-Rengifo, M.J., Duarte, L.V., Goy, A., Paredes, R., Silva, R.L., El Sinemuriense Superior (cronozonas Oxynotum y Raricostatum) en la región de S. Pedro de Moel (Cuenca Lusitánica, Portugal) (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 15-19. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosCorreia, G., Aplicações da Espectrometria de Raios Gama no Estudo de Séries Carbonatadas. O caso do Jurássico Inferior da Bacia Lusitânica (2011) Tese de Mestrado em Geociências (especialidade em Geologia do Petróleo) não publicada, p. 97. , Departamento de Ciências da Terra da Universidade de Coimbra, CoimbraCorreia, G., Duarte, L.V., Pereira, A., Silva, R.L., Outcrop Spectral Gamma-Ray applications to the Sinemurian-Pliensbachian organic-rich facies of the Lusitanian Basin (Portugal) (2012) Journal of Iberian Geology, 38 (2), pp. 373-388Correia, G., Duarte, L.V., Pereira, A.C., Evidências paleogeográficas e correlação estratigráfica através da utilização da espectrometria de raios gama no Toarciano inferior de diferentes sectores da Bacia Lusitânica (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 89-93. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosCorreia, G., Duarte, L.V., Pereira, A.C., Silva, R.L., Mendonça Filho, J.G., Utilização da espectrometria de raios gama na caracterização das fácies ricas em matéria orgânica do Sinemuriano-Pliensbaquiano da Bacia Lusitânica (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 77-82. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosDuarte, L.V., Facies analysis and sequential evolution of the Toarcian-Lower Aalenian series in the Lusitanian Basin (Portugal) (1997) Comunicações do Instituto Geológico e Mineiro, 83, pp. 65-94Duarte, L.V., Lithostratigraphy, sequence stratigraphy and depositional setting of the Pliensbachian and Toarcian series in the Lusitanian Basin (Portugal) (2007) Ciências da Terra (UNL), 16, pp. 17-23Duarte, L.V., Krautter, M., Soares, A.F., Bioconstructions à spongiaires siliceux dans le Lias terminal du Bassin lusitanien (Portugal): Stratigraphie, sédimentologie et signification paléogéographique (2001) Bulletin de la Société Géologique de France, 172 (5), pp. 637-646Duarte, L.V., Silva, R.L., Mendonça Filho, J.G., The Lower Jurassic of west coast of Portugal: Stratigraphy and organic matter in carbonate sedimentation (2011) Field Trip Guide Book of 63rd Annual Meeting of the International Committee for Coal and Organic Petrology, Memórias, 17, pp. 1-42. , In: Flores, M., Marques, M. (Eds)Duarte, L.V., Silva, R.L., Mendonça Filho, J.G., Variação do COT e pirólise Rock-Eval do Jurássico Inferior da região de S. Pedro de Moel. Potencial de geração de hidrocarbonetos (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 107-111. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosDuarte, L.V., Silva, R.L., Mendonça Filho, J.G., Oliveira, L.C., Evidências geoquímicas do Jurássico Inferior de Peniche (Bacia Lusitânica, Portugal): Estado de arte e perspectivas futuras. In: Flores, D., Marques, M. (Eds), Saídas de Campo do X Congresso de Geoquímica dos Países de Língua Portuguesa/XVI Semana de Geoquímica (2010) Memórias (Porto), 15, pp. 3-24Duarte, L.V., Silva, R.L., Mendonça Filho, J.G., Poças Ribeiro, N., Chagas, R.B.A., High-resolution stratigraphy, Palynofacies and source rock potential of the Água de Madeiros Formation (Upper Sinemurian) of the Lusitanian Basin, Portugal (2012) Journal of Petroleum Geology, 35 (2), pp. 105-126Duarte, L.V., Silva, R.L., Mendonça Filho, J.G., Poças Ribeiro, N., Ferreira, F., Chapter 8: Case Studies (Dispersed Organic Matter). Lusitanian Basin: Geological setting, stratigraphy and organic matter record in the Lower Jurassic hemipelagic deposits of the Lusitanian Basin (2011) ICCP Training Course on Dispersed Organic Matter, pp. 107-118. , In: Flores, D., Marques, M. (Eds), ICCP Training program Commission I, PortoDuarte, L.V., Silva, R.L., Oliveira, L.C.V., Comas-Rengifo, M.J., Silva, F., Organic-rich facies in the Sinemurian and Pliensbachian of the Lusitanian Basin, Portugal: Total Organic Carbon distribution and relation to transgressive-regressive facies cycles (2010) Geologica Acta, 8, pp. 325-340Duarte, L.V., Soares, A.F., Litostratigrafia das séries margocalcárias do Jurássico Inferior da Bacia Lusitânica (Portugal) (2002) Comunicações do Instituto Geológico e Mineiro, 89, pp. 135-154Ferreira, R., Composição orgânica e maturação térmica da Formação de Vale das Fontes (Pliensbaquiano) de Peniche. Potencial de geração de hidrocarbonetos (2010) Tese de Mestrado em Geociências (especialidade em Geologia do Petróleo) não publicada, p. 89. , Departamento de Ciências da Terra da Universidade de Coimbra, CoimbraFerreira, R., Mendonça Filho, J.G., Duarte, L.V., Silva, R.L., Mendonça, J.O., Hydrocarbon generation potential of the Pliensbachian organic-rich series of Peniche (Lusitanian Basin, Portugal): An organopetrographic and thermal maturation assessment integrated analysis (2010) II Central & North Atlantic Conjugate Margins Conference. Extended Abstracts, Lisbon, pp. 116-120. , In: Pena dos Reis, R, Pimentel, N. (Eds), PortugalLoureiro, I.M., Cabral, M.C., Duarte, L.V., Azerêdo, A.C., Ostracodos do Sinemuriano superior da região de S. Pedro de Moel e de Peniche: Relação com o contexto sedimentar (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 49-54. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosMattioli, E., Plancq, M., Boussaha, M., Duarte, L.V., Pittet, B., Calcareous nannofossil biostratigraphy: New data from the Early Jurassic of the Lusitanian Basin (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 69-76. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosMendonça Filho, J.G., Silva, T.F., Silva, R.L., Duarte, L.V., Poças Ribeiro, N., Distribuição dos biomarcadores hopanóides e sua relação com a matéria orgânica nas séries jurássicas de S. Pedro de Moel, Bacia Lusitânica (Portugal) (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 101-105. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosMouterde, R., Rocha, R.B., Ruget, C., Tintant, H., Faciès, biostratigraphie et paléogéographie du Jurassique portugais (1979) Ciências Terra (UNL), 5, pp. 29-52Mouterde, R., Ruget, C., Esquisse de la paléogéographie du Jurassique inférieur et moyen au Portugal (1975) Bulletin de la Société géologique de France, 17, pp. 779-786. , 7e SérieParedes, R., Bivalves do Sinemuriano da Bacia Lusitânica. 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Novos contributos paleontológicos, sedimentológicos e geoquímicosSilva, R.L., Duarte, L.V., Mendonça Filho, J.G., Optical and geochemical characterization of Upper Sinemurian (Lower Jurassic) fossil wood from the Lusitanian Basin (Portugal) (2013) Geochemical Journal, , (em revisão)Silva, R.L., Duarte, L.V., Mendonça Filho, J.G., Azerêdo, A.C., Contrasts between the two main Jurassic source rocks in the western margin of the Lusitanian Basin (Portugal) (2010) II Central & North Atlantic Conjugate Margins Conference, pp. 261-265. , In: Pena dos Reis, R, Pimentel, N (Eds.), Extended Abstracts, Lisbon, PortugalSilva, R.L., Mendonça Filho, J.G., Azerêdo, A.C., Duarte, L.V., Palynofacies and TOC analysis of marine and non-marine sediments across the Middle-Upper Jurassic boundary in the Central-Northern Lusitanian Basin (Portugal) (2013) Facies, , doi: 10.1007/s10347-013-0369-xSilva, R.L., Mendonça Filho, J.G., Azerêdo, A.C., Duarte, L.V., Palinofácies e caracterização de matéria orgânica da Formação de Cabaços (Bacia Lusitânica): Contributos para a discriminação das dinâmicas paleobiológicas e paleoambientais em meios carbonatados dominantemente não-marinhos (2013) Comunicações Geológicas, 100 (ESPECIAL I), pp. 113-118. , In: Duarte, L.V., Silva, R.L., Azerêdo, A.C. (Eds), Fácies carbonatadas ricas em matéria orgânica do Jurássico da Bacia Lusitânica. Novos contributos paleontológicos, sedimentológicos e geoquímicosSilva, R.L., Mendonça Filho, J.G., da Silva, F.S., Duarte, L.V., Silva, T.F., Ferreira, R., Azerêdo, A.C., Can biogeochemistry aid in the palaeoenvironmental/early diagenesis reconstruction of the ~187 Ma (Pliensbachian) organic-rich hemipelagic series of the Lusitanian Basin (Portugal)? (2012) Bulletin of Geosciences, 87, pp. 373-382Soares, A.F., Rocha, R.B., Elmi, S., Henriques, M.H., Mouterde, R., Almeras, Y., Ruget, C., Kullberg, J.C., Le sous-bassin nord-lusitanien (Portugal) du Trias au Jurassique moyen: Histoire d'un "rift avorté" (1993) Comptes rendus de l'Académie des sciences de Paris, 317, pp. 1659-166