As sete vidas de Adília Lopes ou o renascer da “mulher-osga”

O presente artigo pretende refletir sobre o conceito de Vita Nova perspetivado por Roland Barthes e associá-lo à obra de Adília Lopes, poetisa portuguesa contemporânea. Focar-se-á, em particular, a relação entre vida “real”, escrita poética e a imagem cultivada pela autora através dos seus textos e paratextos

Lipidomics reveals similar changes in serum phospholipid signatures of overweight and obese pediatric subjects

Obesity is a public health problem and a risk factor for pathologies such type 2 diabetes mellitus, cardiovascular diseases, and nonalcoholic fatty liver disease. Given these clinical implications, there is a growing interest to understand the pathophysiological mechanism of obesity. Changes in lipid metabolism have been associated with obesity and obesity-related complications. However, changes in the lipid profile of obese children have been overlooked. In the present work, we analyzed the serum phospholipidome of overweight and obese children by HILIC-MS/MS and GC-MS. Using this approach, we have identified 165 lipid species belonging to the classes PC, PE, PS, PG, PI, LPC, and SM. The phospholipidome of overweight (OW) and obese (OB) children was significantly different from normal-weight children (control). Main differences were observed in the PI class that was less abundant in OW and OB children and some PS, PE, SM, and PC lipid species are upregulated in obese and overweight children. Although further studies are needed to clarify some association between phospholipid alterations and metabolic changes, our results highlight the alteration that occurs in the serum phospholipid profile in obesity in children.publishe

Genome-wide profiling of p53-regulated enhancer RNAs uncovers a subset of enhancers controlled by a lncRNA

p53 binds enhancers to regulate key target genes. Here, we globally mapped p53-regulated enhancers by looking at enhancer RNA (eRNA) production. Intriguingly, while many p53-induced enhancers contained p53-binding sites, most did not. As long non-coding RNAs(lncRNAs) are prominent regulators of chromatin dynamics, we hypothesized that p53-induced lncRNAs contribute to the activation of enhancers by p53. Among p53-induced lncRNAs, we identified LED and demonstrate that its suppression attenuates p53 function. Chromatin-binding and eRNA expression analyses show that LED associates with and activates strong enhancers. One prominent target of LED was located at an enhancer region within CDKN1A gene, a potent p53-responsive cell cycle inhibitor. LED knockdown reduces CDKN1A enhancer induction and activity, and cell cycle arrest following p53 activation. Finally, promoter-associated hypermethylation analysis shows silencing of LED in human tumours. Thus, our study identifies a new layer of complexity in the p53 pathway and suggests its dysregulation in cancer

Influenza severe cases in hospitals, between 2014 and 2016 in Portugal

Rede Portuguesa de Laboratórios para o Diagnóstico da GripeBackground: Since 2009, the Portuguese Laboratory Network (PLNID) for Influenza Diagnosis has integrated 15 Laboratories in mainland and Atlantic Islands of Azores and Madeira. This PLNID added an important contribute to the National Influenza Surveillance Program regarding severe and hospitalized influenza cases. The present study aims to describe influenza viruses detected in influenza like illness (ILI) cases: outpatients (Outp), hospitalized (Hosp), and intensive care units (ICU), between 2014 and 2016. Methods: The PLNID performs influenza virus diagnosis by biomolecular methodologies. Weekly reports to the National Influenza Reference Laboratory ILI cases tested for influenza. Reports include data on detecting viruses, hospital assistance, antiviral therapeutics, and information on death outcome. Were reported during two winter seasons 8059 ILI cases,being 3560 cases in 2014/15 (1024 in Outp, 1750 Hosp, and 606 in ICU) and 4499 cases in 2015/2016 (1933 in Outp, 1826 Hosp, and 740 in ICU). Results: The higher percentage of influenza positive cases were detected in Outp in both seasons, 18% during 2014/15 and 20% in 2015/16. In 2014/15,influenza cases were more frequent in individuals older than 65 years old and these required more hospitalizations,even in ICU. In 2015/16,the influenza cases were mainly detected in individuals between 15-64 years old. A higher proportion of influenza positive cases with hospitalization in ICU were observed in adults between 45-64 years old.During the study period,the predominant circulating influenza viruses were different in the two seasons: influenza B and A(H3) co-circulated in 2014/15,and influenza A(H1)pdm09 was predominant during 2015/16. Even when influenza A is notthe dominant virus, A(H3) and A(H1)pdm09 subtypes correlate with higher detection rate in hospitalized cases (Hosp and UCI), with higher frequencies in adults older than 45. Influenza B,detected in higher proportion in outpatients, was frequently relatedwith influenza cases in younger age groups: 0-4 and 5-14 years old. Conclusions: This study highlights the correlation of theinfluenza virus type/subtype that circulates in each season with the possible need for hospitalization and intensive care in special groups of the population. Circulation of influenza A subtypes can cause more frequentdisease in individuals older than 45, with need of hospitalization including intensive care. On the other hand, influenza B is more frequently associated with less severe cases and with infection in children and younger adults. Influenza B circulation might predict lower number of hospitalizations.The identification of influenza type in circulation,byPLNID ineach season, could guide action planning measures in population health care.info:eu-repo/semantics/publishedVersio

Dinâmicas de (investig)ação em contextos de promoção da língua portuguesa: a presença do CIDTFF na rede Camões, I.P.

Sem resumo disponível.publishe

N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration

Background: non-coding RNAs have been drawing increasing attention in recent years as functional data suggest that they play important roles in key cellular processes. N-BLR is a primate-specific long non-coding RNA that modulates the epithelial-to-mesenchymal transition, facilitates cell migration, and increases colorectal cancer invasion. Results: we performed multivariate analyses of data from two independent cohorts of colorectal cancer patients and show that the abundance of N-BLR is associated with tumor stage, invasion potential, and overall patient survival. Through in vitro and in vivo experiments we found that N-BLR facilitates migration primarily via crosstalk with E-cadherin and ZEB1. We showed that this crosstalk is mediated by a pyknon, a short ~20 nucleotide-long DNA motif contained in the N-BLR transcript and is targeted by members of the miR-200 family. In light of these findings, we used a microarray to investigate the expression patterns of other pyknon-containing genomic loci. We found multiple such loci that are differentially transcribed between healthy and diseased tissues in colorectal cancer and chronic lymphocytic leukemia. Moreover, we identified several new loci whose expression correlates with the colorectal cancer patients' overall survival. Conclusions: the primate-specific N-BLR is a novel molecular contributor to the complex mechanisms that underlie metastasis in colorectal cancer and a potential novel biomarker for this disease. The presence of a functional pyknon within N-BLR and the related finding that many more pyknon-containing genomic loci in the human genome exhibit tissue-specific and disease-specific expression suggests the possibility of an alternative class of biomarkers and therapeutic targets that are primate-specific

The dynamic DNA methylomes of double-stranded DNA viruses associated with human cancer

The natural history of cancers associated with virus exposure is intriguing, since only a minority of human tissues infected with these viruses inevitably progress to cancer. However, the molecular reasons why the infection is controlled or instead progresses to subsequent stages of tumorigenesis are largely unknown. In this article, we provide the first complete DNA methylomes of double-stranded DNA viruses associated with human cancer that might provide important clues to help us understand the described process. Using bisulfite genomic sequencing of multiple clones, we have obtained the DNA methylation status of every CpG dinucleotide in the genome of the Human Papilloma Viruses 16 and 18 and Human Hepatitis B Virus, and in all the transcription start sites of the Epstein-Barr Virus. These viruses are associated with infectious diseases (such as hepatitis B and infectious mononucleosis) and the development of human tumors (cervical, hepatic, and nasopharyngeal cancers, and lymphoma), and are responsible for 1 million deaths worldwide every year. The DNA methylomes presented provide evidence of the dynamic nature of the epigenome in contrast to the genome. We observed that the DNA methylome of these viruses evolves from an unmethylated to a highly methylated genome in association with the progression of the disease, from asymptomatic healthy carriers, through chronically infected tissues and pre-malignant lesions, to the full-blown invasive tumor. The observed DNA methylation changes have a major functional impact on the biological behavior of the viruses

Des-Dobra: re-visão e tradução. A construção da poetisa em Adília Lopes = Des-Pliegue: re-visión y traducción. La construcción de la poetisa en Adília Lopes

[por] É nosso propósito, neste trabalho doutoral, ler a obra da poetisa portuguesa contemporânea Adília Lopes (Lisboa, 1960), a partir de uma perspetiva ginocrítica, suportada pelos Estudos de Género, e mais particularmente Feministas, refletindo, simultânea e consequentemente, sobre as questões levantadas pela escrita de mulheres, assim como apresentar uma leitura inserida num quadro de transcriação tradutológica. O projeto literário Adília Lopes raramente surge associado a um pensamento feminista ou politicamente interventivo. O presente estudo tem por objetivo principal sublinhar, precisamente, as relações entre a componente intervencionista e de luta do feminismo e o empenho da artista/cidadã na construção uma sociedade igualitária. Aceitando a perspetiva ginocrítica -na esteira de Elaine Showalter, Hélène Cixous ou Anna Klobucka- como modelo interpretativo que tem por objeto a mulher enquanto produtora de significado textual e, simultaneamente, como paradigma científico e académico, a primeira parte do nosso trabalho atenta na construção da autora de poesia, a poetisa, através do uso da pseudonímia feminina e da figura da autobiografia, concluindo da sua eficácia enquanto ato de resistência e denúncia dos jogos de autoridade e poder, baseados na dicotomia feminino / masculino. Neste situar do sujeito escritora em contexto, abordam-se as representações do corpo na idade pós-moderna na qual inscrevemos a nossa autora. Desta forma, destacamos o modo como se entrelaça, de forma complexa, a urdidura do pensamento feminista e pós-feminista com a conceção pós-modernista da história, da sociedade e do homem. Considerando a escrita adiliana um produto do seu tempo, estabelecemos as ligações da poesia de Lopes ao marco tipológico representado pelo pós-modernismo. Contudo, antes de procedermos à ilustração e à análise da forma como as coordenadas deste movimento se consubstanciam na obra adiliana, examinamos as grandes etapas do percurso histórico deste conceito periodológico. Neste sentido, damos especial destaque à sua perigosa ligação com os feminismos que entendemos plurais e diversos. Num segundo momento do nosso estudo, atentamos nas linhas de força da escrita de Adília Lopes, reveladas pela ferramenta intertextual e pelo seu diálogo incessante e peculiar com o mundo. Inscrevemos as histórias de Adília e Maria José nas narrativas feministas, destacando a politização da sua escrita, oriunda de uma personalização identitária que age como uma âncora que a localiza no seu corpo, na sua rua, na cidade, no país, no mundo. Vemos, por fim, de que forma a excessiva pessoalização do sujeito poético adiliano torna a sua escrita impessoal, no sentido dado por Roberto Esposito, a partir da reflexão da filósofa Simone Weil. A terceira pessoa, o terceiro corpo que emerge desta desagregação pessoal, celebra a comunhão cristã e redentora de Adília Lopes com o mundo dos homens. Na última parte deste trabalho académico, e propondo uma outra forma de leitura, apresentamos a tradução para castelhano do Clube da Poetisa Morta, publicado em 1997. Na perspetiva hermenêutico-tradutológica adotada, a tradução do texto literário apresenta-se sempre como uma re-visão criativa do texto original e um desvelamento ou uma instauração de novos horizontes de sentido. A tradutora assentará o seu plano de tradução no conceito de “projeto menorizante”, segundo o formato proposto por Lawrence Venuti, e privilegiará o “écart” (desvio) em detrimento da diferença textual, comungando assim com a reflexão filosófica de François Jullien a propósito do desvio e da diferença. O livro selecionado anuncia, desde o título, várias linhas de leitura presentes na obra adiliana, sejam a ironia iconoclasta, a temática da morte (figurada e literal), a miséria humana, motivo fundamental na poesia impessoal da autora, passando pelas referências à cultura popular, à religião católica e a culturemas específicos que fazem deste conjunto poemático um exemplo da escrita distintiva da poetisa portuguesa.[eng] This dissertation aims to read the poetry of the Portuguese author Adília Lopes (Lisbon, 1960) from a gynocritical perspective rooted in Gender Studies and more precisely Women’s Studies. It analyzes questions raised by women’s writing and, as a different form of reading, offering a recreation based on translation. At her début, Adília Lopes was only published by marginal editors and was labeled as an eccentric author whose writing could not be thought as poetry. In spite of the belated interest, the author is now recognized by scholars and renown editors, such as Assírio & Alvim. Nevertheless, the literary project named Adília Lopes was not often associated with a feminist or even political thought. For this reason, this study intends to reveal that Lopes’ poetry postulates a third way, a third body personified in writing and which resists the classifications and the impositions of a cruel, unequal, and profoundly individualistic society. Through her impersonal stylistic mode, the author’s poetry inscribes itself within both the necessary revision of past models and a new textual perspective. Reading the Adilian canon in the light of a gynocritical paradigm which fixes woman as a subject, rather than as a mere object, we find the foundations and processes that construct the feminine and the masculine in the poetic tissue, as they are represented in Lopes’ poetry, in the author’s ironic handling of stereotypes and clichés. Considering the close relation between this theoretical framework and new approaches to the postmodern lyric subject, this thesis also investigates the fabrication of a textual identity for a lyrical self, understood as the construction of the woman who writes poetry, that is, the composition of the poetess. The final part of the thesis proposes another reading practice promotted by the exercise in translating Adília Lopes’ book Clube da Poetisa Morta (“Dead poetess society”) into Spanish. With this purpose, the study reflects on translation as an intercultural, interpersonal and “inter-authorial” act. It focuses, thus, on some generic aspects about translation and, specifically, the translation of poetry. The woman translator that consciously undertakes the role of translator privileges a “minoritizing project” as defined by Venuti and centers on the notion of “écart” (deviation) to the detriment of the textual difference

Are intermediate school meals a real contribution to improve a healthy and sustainable diet ?

Purpose The purpose is to analyse the nutritional adequacy and carbon footprint of intermediated meals provided to preschool children and primary-level students in a Portuguese municipality. Design/methodology/approach An observational cross-sectional study was conducted with a convenience sampling consisting of school snacks from a Portuguese municipality. The nutritional assessment used food labels and a Portuguese food composition table. The literature review for carbon footprint assessment was conducted by searching for the products under analysis or similar ones. Findings The results showed that 80% of snacks have a higher energy value than recommended. The majority of options are below recommendations for protein and fat and above recommendations for carbohydrates. The intermediated meals with more dairy products in composition have the highest carbon footprint. The carbon footprint included the packaging of the products, and it wasn't possible to determine the influence of non-food products. Research limitations/implications This study has limitations in the fact that we do not know the carbon footprint of Portuguese products and we had to compare them with others, from different countries, with possibly different types of production. Practical implications Intermediate meals are inadequate, and the carbon footprint is higher when the intermediated meals include products of animal origin – the reason why the composition of intermediated meals should be redesigned considering the achievement of these targets. Social implications The promotion of intermediated meals that promote the Mediterranean eating pattern contributes to health and well-being and is a vehicle for nutrition education and healthy food consumption in schools. Originality/value Many studies have been conducted to analyse the carbon footprint and environmental impact of school meals, but commonly lunch is the meal evaluated and the assessment of intermediated meals' impact is an open field. Originality/value Many studies have been conducted to analyse the carbon footprint and environmental impact of school meals, but commonly lunch is the meal evaluated and the assessment of intermediated meals' impact is an open field.info:eu-repo/semantics/publishedVersio

Insuficiência renal aguda e febre prolongada – um caso de nefrite intersticial aguda induzida por fármacos

A nefrite intersticial aguda (NIA) é uma causa de insuficiência renal aguda em idade pediátrica. A etiologia medicamentosa é a mais frequente e o tratamento primário da NIA consiste na eliminação do agente etiológico suspeito. Descreve-se o caso de uma adolescente, de 15 anos, com uma síndrome febril com 24 dias de evolução, acompanhado de exantema maculopapular e insuficiência renal aguda (IRA). Após exclusão de etiologias mais frequentes, foi diagnosticada NIA induzida por fármacos e após descontinuação da terapêutica evoluiu favoravelmente. A NIA tem geralmente um prognóstico favorável, mas pode progredir com IRA e, raramente, para nefrite crónica, sendo fundamental o seu diagnóstico precoce bem como a identificação do agente agressor