A Question of G-d: Jewish Theology and Memoirs of the Holocaust

The Holocaust, the systematic murder of the European Jews by the Germans, had massive impacts on the religious beliefs of those Jews who survived it. Nazi authorities and their accomplices stripped Jews away from their homes, their families, and everything they knew. Forced to work under inhumane conditions, many came to question the God they had followed and the religion they had practiced. This thesis investigates the memoirs of five Jewish survivors to analyze the impact the Holocaust had on their faith

Assessing Medical Room Behavior During Infants’ Painful Medical Procedures: The Measure of Adult and Infant Soothing and Distress (MAISD)

This study evaluated the Measure of Adult and Infant Soothing and Distress (MAISD) for examining infant, parent, and nurse behavior during infants’ immunizations. Videotapes of 62 infants, parents, and nurses during immunizations were coded. Concurrent validity and reliability for the MAISD were demonstrated. The scale revealed that infants displayed predominately distress, and adults exhibited primarily reassurance. Parents’ and nurses’ distractions were positively related to infants’ engaging in distraction, and parents’ and nurses’ reassurance was positively associated with infant distress. There appear to be avenues in which to intervene to teach parents and nurses how to best behave to help infants during their painful medical events

Safe and Sustainable Commutes

A group of six undergraduate students worked with a local city government, their professor, and other university leaders to develop a research project related to sustainable community transportation. A city road project was analyzed to understand the sustainability implications to a changing transportation system. By taking a closer look at the city and the adjacent residential community, the project was examined. Additionally, a university project was undertaken to investigate the adoption of an alternative transportation resource on campus. With this information gathered, the campus community has the ability to further utilize new resources to improve campus transportation sustainability

DNM1 encephalopathy: A new disease of vesicle fission.

ObjectiveTo evaluate the phenotypic spectrum caused by mutations in dynamin 1 (DNM1), encoding the presynaptic protein DNM1, and to investigate possible genotype-phenotype correlations and predicted functional consequences based on structural modeling.MethodsWe reviewed phenotypic data of 21 patients (7 previously published) with DNM1 mutations. We compared mutation data to known functional data and undertook biomolecular modeling to assess the effect of the mutations on protein function.ResultsWe identified 19 patients with de novo mutations in DNM1 and a sibling pair who had an inherited mutation from a mosaic parent. Seven patients (33.3%) carried the recurrent p.Arg237Trp mutation. A common phenotype emerged that included severe to profound intellectual disability and muscular hypotonia in all patients and an epilepsy characterized by infantile spasms in 16 of 21 patients, frequently evolving into Lennox-Gastaut syndrome. Two patients had profound global developmental delay without seizures. In addition, we describe a single patient with normal development before the onset of a catastrophic epilepsy, consistent with febrile infection-related epilepsy syndrome at 4 years. All mutations cluster within the GTPase or middle domains, and structural modeling and existing functional data suggest a dominant-negative effect on DMN1 function.ConclusionsThe phenotypic spectrum of DNM1-related encephalopathy is relatively homogeneous, in contrast to many other genetic epilepsies. Up to one-third of patients carry the recurrent p.Arg237Trp variant, which is now one of the most common recurrent variants in epileptic encephalopathies identified to date. Given the predicted dominant-negative mechanism of this mutation, this variant presents a prime target for therapeutic intervention

Mapping the Hsp90 Genetic Interaction Network in Candida albicans Reveals Environmental Contingency and Rewired Circuitry

The molecular chaperone Hsp90 regulates the folding of diverse signal transducers in all eukaryotes, profoundly affecting cellular circuitry. In fungi, Hsp90 influences development, drug resistance, and evolution. Hsp90 interacts with ∼10% of the proteome in the model yeast Saccharomyces cerevisiae, while only two interactions have been identified in Candida albicans, the leading fungal pathogen of humans. Utilizing a chemical genomic approach, we mapped the C. albicans Hsp90 interaction network under diverse stress conditions. The chaperone network is environmentally contingent, and most of the 226 genetic interactors are important for growth only under specific conditions, suggesting that they operate downstream of Hsp90, as with the MAPK Hog1. Few interactors are important for growth in many environments, and these are poised to operate upstream of Hsp90, as with the protein kinase CK2 and the transcription factor Ahr1. We establish environmental contingency in the first chaperone network of a fungal pathogen, novel effectors upstream and downstream of Hsp90, and network rewiring over evolutionary time

The genomic landscape of balanced cytogenetic abnormalities associated with human congenital anomalies

Despite the clinical significance of balanced chromosomal abnormalities (BCAs), their characterization has largely been restricted to cytogenetic resolution. We explored the landscape of BCAs at nucleotide resolution in 273 subjects with a spectrum of congenital anomalies. Whole-genome sequencing revised 93% of karyotypes and demonstrated complexity that was cryptic to karyotyping in 21% of BCAs, highlighting the limitations of conventional cytogenetic approaches. At least 33.9% of BCAs resulted in gene disruption that likely contributed to the developmental phenotype, 5.2% were associated with pathogenic genomic imbalances, and 7.3% disrupted topologically associated domains (TADs) encompassing known syndromic loci. Remarkably, BCA breakpoints in eight subjects altered a single TAD encompassing MEF2C, a known driver of 5q14.3 microdeletion syndrome, resulting in decreased MEF2C expression. We propose that sequence-level resolution dramatically improves prediction of clinical outcomes for balanced rearrangements and provides insight into new pathogenic mechanisms, such as altered regulation due to changes in chromosome topology

Correction to: Cluster identification, selection, and description in Cluster randomized crossover trials: the PREP-IT trials

An amendment to this paper has been published and can be accessed via the original article

Overwintering Biology of Culex Pipiens Linnaeus (Culicidae) in East-Central Illinois

136 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.Culex pipiens was the only species collected from the Champaign-Urbana, overwintering site. Temperature was the key factor in resting site choice by these females, with most found in the coldest areas of the site throughout the overwintering season. Furthermore, results suggested that temperature affected both follicle state and the quantity and quality of depot fats in overwintering females. The general trend in mean primary follicle lengths of females collected from the site was an increase in size from November to March. However, significant decreases in follicle length from January to February were noted for females collected from the coldest area of the site, corresponding to significant temperature decreases in this area during that time. Culex pipiens collected from October to January had very similar mean total fat values with only the value for March significantly less than values of females collected during previous months. Total fat values for mosquitoes collected from the warmest and coldest sections during January, February, and March were not significantly different. However, the results showed that females from the coldest area of the site lost their initial fat reserves at a slower rate than those collected from the warmest area. Gas-liquid chromatography (GLC) showed the primary fatty acid in these females was the monounsaturate palmitoleic acid. Mosquitoes collected from the coldest section of the site in January had a greater mean percentage of palmitoleic acid than those collected from the warmest area. This suggested that, while females from the different sections had similar mean amounts of fat during this month, the qualities of their fat reserves were different.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD

A Single Application of Compost Can Leave Lasting Impacts on Soil Microbial Community Structure and Alter Cross-Domain Interaction Networks

Our current understanding suggests that nutrient management strategies applied to agricultural soils over multiple years are required to cause major and stable shifts in soil microbial communities. However, some studies suggest that agricultural soils can benefit even from sporadic, single additions of organic matter. Here we investigate how single additions of high-quality organic matter can cause significant shifts in microbial soil communities over multiple cropping cycles. We grew radishes in a tropical Oxisol soil for six crop cycles after a single application of a high-nitrogen compost or urea. At planting and before biomass harvest, we sampled soils influenced by the radish rhizosphere and sequenced bacterial and archaeal 16S and fungal ITS rDNA marker genes. We measured microbial richness and diversity, community composition and structure, and constructed correlation networks to predict cross-domain microbial interactions. We found that a single application of compost, compared to urea or control, resulted in a persistent improved plant biomass response and led to sustained changes in the soil microbial community throughout the duration of the 227-day study. Compost altered the structure of both the fungal and prokaryotic microbial communities, introduced new microorganisms that persisted in the resident soil system, and altered soil microbial correlation network structure and hub taxa. In contrast, fertilization with urea did not significantly alter the structure of soil microbial communities compared to the control but reduced network complexity and altered hub taxa. This study highlights the significant impacts that high-quality organic matter fertilization can exert on agricultural soil microbiomes and adds to the growing body of knowledge on using organic fertilizers as a way to steer the soil microbiome toward a healthier soil