Análisis genético y molecular del gen bHLH149 en la respuesta al ayuno de fosfato

Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología. Fecha de lectura: 18-11-201

How is educational legislation mirrored in textbooks? A mixed-method study in relation to the minimum contents of music education in Early Childhood Education (3-6 years) = ¿Cómo se refleja la legislación educativa en los libros de texto? Un estudio de metodología mixta en relación a los contenidos mínimos de educación musical en el segundo ciclo (3-6 años) de educación infantil

Although there are many works dedicated to the study of either educational legislation or textbooks, only a few are devoted to their joint research. In the present study, we aimed to investigate how the national regulations regarding the mandatory contents related to music in Early Childhood Education in the stage of 3 to 6 years are reflected in textbooks in Spain. By means of a multistage mixed methodology, including analyses based on grounded theory, content analysis, as well as cluster and variance analyses, our results provide evidence for: 1) a modern music education philosophy underlying the national Spanish regulations, 2) discrepancies between this philosophy and its development in textbooks, and 3) the existence of different publisher profiles regarding the treatment of contents in music. Finally, we discuss the implications of our results regarding the use of textbooks at this educational stage and in relation to music education

Recuperación de las interacciones entre el haya (Fagus sylvatica) y los hongos ectomicorrícicos 140 años después del fin de la actividad minera

Even the increasing use of restoration, it does not always imply a shortterm answer in ecosystems that guarantees the recovery of their structure, functions, and services. So far, most studies evaluating ecosystem recovery have used metrics that ignore the complexity needed to structure communities of organisms that form ecosystems. Here, we analyze the recovery of species interactions (metric with a certain level of complexity) in a large time scale ('100 years). In particular, we characterized, using molecular identification, the ectomicorrhyzal (EcM) fungal communities present in 18 beech trees inside and seven outside an ancient iron in Navarra (northern Spain), in use from the XIV century until 140 years ago, as well as seven beech trees from a nearby oldgrowth forest. Species richness of EcM fungi was similar for the three locations, while differences were found for species composition in the area damaged by mining and compare to outside the mine and the reference beech forest. Our results suggest the need to assess ecosystem recovery with more complex metrics (e.g. architecture of interaction networks) in order to accurately estimate the real time required for ecosystems to fully recover. © 2019 Los Autores.Este trabajo ha sido financiado por el Plan Nacional de Investigación RETOS (CGL2015-70452-R) y la acreditación de excelencia “María de Maeztu” 2018-2022 (MDM-2017-0714). ARU fue financiada por el programa de becas predoctorales (2016) de la Fundación Tatiana Guzman el Bueno

Inflammatory capacity of exosomes released in the early stages of acute pancreatitis predicts the severity of the disease

As acute pancreatitis progresses to the severe form, a life-threatening systemic inflammation is triggered. Although the mechanisms involved in this process are not yet well understood, it has been proposed that circulating exosomes may be involved in the progression of inflammation from the pancreas to distant organs. Here, the inflammatory capacity and protein profile of plasma exosomes obtained during the first 24 h of hospitalization of patients diagnosed with acute pancreatitis were characterized and compared with the final severity of the disease. We found that the final severity of the disease strongly correlates with the inflammatory capacity of exosomes in the early stages of acute pancreatitis. Exosomes isolated from patients with mild pancreatitis had no effect on macrophages, while exosomes isolated from patients with severe pancreatitis triggered NFκB activation, TNFα and IL1β expression, and free radical generation. To delve deeper into the mechanism involved, we performed a proteomic analysis of the different exosomes that allowed us to identify different groups of proteins whose concentration was also correlated with the clinical classification of pancreatitis. In particular, an increase in the amount of S100A8 and S100A9 carried by exosomes of severe pancreatitis suggests that the mechanism of action of exosomes is mediated by the effect of these proteins on NADPH oxidase. This enzyme is activated by S100A8/S100A9, thus generating free radicals and promoting an inflammatory response. Along these lines, we observed that inhibition of this enzyme abolished all the pro-inflammatory effects of exosomes from severe pancreatitis. All this suggests that the systemic effects, and therefore the final severity of acute pancreatitis, are determined by the content of circulating exosomes generated in the early hours of the process. © 2021 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland.This work was supported by the projects PI16/00060 from Instituto de Salud Carlos III, 2019AEP057 CVSCI, and a grant ‘Gonzalo Miño’ from the Asociación Española de Gastroenterología. The Biologial and Environmental Proteomics group is a member of Proteored-PRB3 and is supported by Grant PT17/0019/0008 of the PE I+D+I 2013–2016, funded by ISCIII and FEDER

Uncontrolled donation after circulatory death: A cohort study of data from a long‐standing deceased‐donor kidney transplantation program.

Despite good long-term outcomes of kidney transplants from controlled donation after circulatory death (DCD) donors, there are few uncontrolled DCD (uDCD) programs. This longitudinal study compares outcomes for all uDCD (N = 774) and all donation after brain death (DBD) (N = 613) kidney transplants performed from 1996 to 2015 at our center. DBD transplants were divided into those from standard-criteria (SCD) (N = 366) and expanded-criteria (N = 247) brain-dead donors (ECD). One-, 5-, and 10-year graft survival rates were 91.7%, 85.7%, and 80.6% for SCD; 86.0%, 75.8%, and 61.4% for ECD; and 85.1%, 78.1%, and 72.2% for uDCD, respectively. Graft survival was worse in recipients of uDCD kidneys than of SCD (P = .004) but better than in transplants from ECD (P = .021). The main cause of graft loss in the uDCD transplants was primary nonfunction. Through logistic regression, donor death due to pulmonary embolism (OR 4.31, 95% CI 1.65-11.23), extrahospital CPR time ≥75 minutes (OR1.94, 95%CI 1.18-3.22), and in-hospital CPR time ≥50 minutes (OR 1.79, 95% CI 1.09-2.93) emerged as predictive factors of primary nonunction. According to the outcomes of our long-standing kidney transplantation program, uDCD could help expand the kidney donor pool.post-print1,71 M

Toll-like receptors in acute kidney injury

Acute kidney injury (AKI) is an important health problem, affecting 13.3 million individuals/year. It is associated with increased mortality, mainly in low- and middle-income countries, where renal replacement therapy is limited. Moreover, survivors show adverse long-term outcomes, including increased risk of developing recurrent AKI bouts, cardiovascular events, and chronic kidney disease. However, there are no specific treatments to decrease the adverse consequences of AKI. Epidemiological and preclinical studies show the pathological role of inflammation in AKI, not only at the acute phase but also in the progression to chronic kidney disease. Toll-like receptors (TLRs) are key regulators of the inflammatory response and have been associated to many cellular processes activated during AKI. For that reason, a number of anti-inflammatory agents targeting TLRs have been analyzed in preclinical studies to decrease renal damage during AKI. In this review, we updated recent knowledge about the role of TLRs, mainly TLR4, in the initiation and development of AKI as well as novel compounds targeting these molecules to diminish kidney injury associated to this pathological conditionThe authors work has been supported by grants from Instituto de Salud Carlos III (ISCIII, FIS-FEDER PI17/00130, PI17/01495, PI20/00375, PI20/00487), Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM) and Cardiovascular (CIBERCV), Spanish Ministry of Science and Innovation (RTI2018-099114-B-100, RTI2018-098788-B-100, DTS19/00093, RYC-2017-22369), and Spanish Societies of Cardiology (SEC), Nephrology (SEN) and Atherosclerosis (SEA). The “PFIS” and “Sara Borrell” training program of the ISCIII supported the salary of MGH (FI18/00310), SR-M (CD19/00021) and CH-B (CP16/00017). Córdoba University supported the salary of C.G.C

Impact of CoQ deficiency on embryonic development in zebrafish

Trabajo presentado en EMBO Workshop. Developmental metabolism: flows of energy, matter, and information, celebrado en Heidelberg (Alemania) del 12 al 15 de septiembre de 2023.Coenzyme Q (CoQ) is a redox-active lipid with a prominent role in the mitochondrial respiratory chain. CoQ is also involved in other redox processes being the electron acceptor for specific mitochondrial dehydrogenases. Primary CoQ deficiencies are rare mitochondrial conditions, biochemically characterised by a reduction in CoQ, caused by biallelic mutations in any of the -at least- 11 COQ genes participating in its biosynthesis. Remarkably, patients show a broad spectrum of clinical manifestations, severity, and age of onset, but a clear genotype-phenotype correlation is still lacking. We hypothesise that the disease unfolding due to defects in specific COQ genes could be different during development and would determine severity, the age of onset and the affected tissues. Modelling rare diseases is a promising approach to overcoming the lack of epidemiological studies. Danio rerio (zebrafish) is a convenient vertebrate model to study embryogenesis due to its straightforward genetic manipulation, the highly efficient, external and easy-to-control oocyte fertilisation and their transparent embryos that make them easy to monitor. We have generated a collection of CRISPR-Cas9 F0 knockout zebrafish models carrying a high rate of biallelic mutations in all known genes involved in CoQ biosynthesis. These somatic F0 mutants enable a high throughput evaluation of loss-of-function phenotypes during early development. Moreover, we have generated a stable coq6 zebrafish knockout line which will allow us to monitor the unfolding of the disease at later embryonic stages. Our work will contribute to close the gap between the knowledge of the regulation of CoQ biosynthesis during development and its coordination with mitochondrial biogenesis. The functional and physiological characterisation of our animal models will help to better understand CoQ deficiencies in humans.Peer reviewe

Repositorio documental de Fisiología Vegetal

Memoria ID-299. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2013-2014.[ES] Este proyecto docente ha servido para la creación de un repositorio documental de fisiología y patología molecular de plantas orientado al aprendizaje e identificación de los fenotipos fisiológicos, genéticos y moleculares utilizados en vegetales, empleando mutantes y plantas transgénicas. Además se han seleccionado, digitalizado e integrado en una base de datos, imágenes de plantas modelo de experimentación como Arabidopsis en distintas etapas del desarrollo vegetal, imágenes de microscopía óptica y confocal descritas acorde con los objetivos de las distintas asignaturas a las que está orientado el repositorio documental. Parte de este material básico, junto con una selección de documentos externos (páginas web, revistas electrónicas, animaciones, videos técnicos y conferencias) se ha utilizado en el presente curso en las distintas asignaturas del Grado en Biología, Biotecnología y Máster en Agrobiotecnología. El conjunto de los recursos está siendo adaptado a formatos de aprendizaje secuencial tutelado y evaluable que se ha integrado en la plataforma Studium de la Universidad de Salamanca y ha estará plenamente operativo en el segundo cuatrimestre del curso académico 2013-2014. Del mismo modo, parte del material generado ha sido empleado en la construcción de las páginas web del Máster en Agrobiotecnología y del Centro Hispano-Luso de Investigaciones Agrarias (CIALE), así como en los folletos divulgativos del citado Máster