Justifications for differentiated approach in correction of some changes of the immunologic disorders in patients with rapidly progressing generalized parodontitis with different drug susceptibility of the parodontal bacteria

Rapidly progressing generalized perio­dontitis (RPGP) still remains an extremely important medical problem, despite certain achievements in solving most of the issues of etiology, pathogenesis and treatment. The study included 102 patients with rapidly progressing periodontitis of I-II and II-III degrees of severity, with ineffective and inconsistent results of previous treatment who were examined and treated in the clinic of the Department of Surgical Dentistry, Periodontics and Implantology of the SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine». On the basis of the diagnostic and therapeutic measures carried out, it was established that, in patients with rapidly progressing generalized periodontitis, the formation of various drug sensitivity in periodontopathogenic microorganisms to protocol antibacterial therapy is closely associated with immunological protocol diseases. The presence of drug-resistant bacteria in the periodontal tissues is combined with a more pronounced immunodeficiency in the mechanisms of systemic and local protection than when the lesions are colonized by drug-sensitive periodontal microorganisms. The revealed features of immunity disorders in patients with RPGP with drug-resistant infection to conventional antibiotic therapy are manifested by a decrease in CD3+ lymphocytes, cells with cytostatic activity (CD4+, CD16+) expressing receptors for the activation of lymphocytes CD25+, HLA-DR and apoptosis CD95+), against the background of a slight increase in CD20+, CD116+, CD54+, significant inhibition of the synthesis of SIgA, IgM, α-INF, γ-INF; a pronounced increase in the production of pro-inflammatory cytokines IL-1β and TNF-α. The need for a differentiated approach in the correction of immunological disorders in patients with RPGP depending on the detected sensitivity and resistance of periodontal pathogenic bacteria to the protocol generally accepted antibacterial therapy has been clearly proved; this provides a faster relief of the infectious and inflammatory process (on average on 3.6±0.2 day) in more than 90% of cases, normalization of the parameters of immunological reactivity in 93.3% of the observed and stable clinical remission of the disease

The program cyberdiesel for mathematical modeling of fuel supply and local intracylinder processes in a diesel engine with volumetric carburetion

The program CyberDiesel is developed on the basis of complex mathematical model of fuel supply and local intracylinder processes in a diesel engine with volumetric carburetion. The program is intended for solving practical problems of coordinating constructive and adjusting parameters of fuel equipment and combustion chamber of a diesel engine by mathematical modeling methods

The complete mitochondrial genome of the extinct Pleistocene horse (Equus cf. lenensis) from Kotelny Island (New Siberian Islands, Russia) and its phylogenetic assessment

The complete mitochondrial genome from the Pleistocene stallion horse (Equus cf. lenensis) which complete skull was found in 1901 on Kotelny Island (New Siberian Archipelago, Sakha Republic, Russia) is published in this paper. The mitochondrial DNA (mtDNA) is 16,584 base pairs (bp) in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes. The overall base composition of the genome in descending order was 32.3% – A, 28.5% – C, 13.4% – G, 25.8% - T without a significant AT bias of 58.2%.publishedVersio

Апроксимація згортки розподілів Вейбулла за допомогою сплайн-експоненційних розподілів

Calculable technology of spline-exponential approximation to Weibull renewal has proposed. The comparative analysis of results of methods of approximation is conducted with the results of construction of analytical renewal function for the Weibull distribution.Предложена вычислительная технология аппроксимации свёртки распределений Вейбулла с помощью сплайн-экспоненциальных распределений. Проведён сравнительный анализ результатов методов аппроксимации с результатами построения аналитической функции распределения свёртки.Запропоновано обчислювальну технологiю апроксимацiї згортки розподiлів Вейбулла за допомогою сплайн-експоненцiйних розподілів. Проведено порівняльний аналіз результатів апроксимаційних методів з результатами побудови аналітичної функції розподілу згортки.

Structure of information technology for detecting speech defects in speech signal

Описано запропоновану авторами інформаційну технологію виявлення дефектів мовлення, методи фонемної сегментації мовного сигналу, класи дефектів, що виявляються, а також способи виявлення цих дефектів. Надано проектно-системну документацію програмного пакета, в якому реалізовано описані алгоритми.Описана предложенная авторами информационная технология обнаружения дефектов речи, методы фонемной сегментации речевого сигнала, классы дефектов, а также способы выявления этих дефектов. Представлена проектно-системная документация программного пакета, в котором реализованы описанные алгоритмы.The purpose of this paper is to describe the methods and algorithms of information technology proposed by authors for speech defects detection. The task of speech defects detection contains the tasks of segmentation of the speech signal, phoneme classification, as well as comparing the possibly impaired speech with a properly spoken reference signal. Comparisons should be made both for the signal as a whole (detection of missed phonemes) and for each phoneme separately (detection of defective phonemes). The segmentation algorithm used in information technology is based on the assertion that, at interphonemic transitions, the signal undergoes significant changes on many scales at once and is characterized by an increase in wavelet detailing coefficients, while in stationary areas the wavelet coefficients are grouped near certain scales. Phoneme wise presentation of the signal makes it possible to identify defects related to the incorrect pronunciation of the phoneme present in the word - the case where the total number of phonemes in the word remains unchanged. It is also possible to detect complete loss of the phoneme or the appearance of extra phonemes. To map phonemes to references, we use a dynamic timeline transformation algorithm, the vector of parameters of which is the Euclidean distance between the Fourier decomposition bands. In order to determine the degree of proximity of the input to the reference, an additional limitation is proposed, according to which the distance from the input signal to the reference signal should be significantly different from the distances for other files. As a response threshold, 1/3 of the variance of distances between files was used. When the number of phonemes in a word does not match the reference, each phoneme of the reference is mapped to an input file, so that one of the input phonemes is matched, without violating the order of phonemes. If more than one non-identified section in a row is detected during segmentation, the sections are combined and compared to the base of the reference phonemes as one phoneme. If the base of the reference phonemes does not match the nearest result to the adjacent left or right phonemes, the segment of the speech is marked as defective. The described algorithms are implemented as part of information technology for assisting in the formulation of the pronunciation by providing the ability to independently assess the quality of the pronunciation

The first dinosaur egg from the Lower Cretaceous of Western Siberia, Russia

© 2017 Informa UK Limited, trading as Taylor & Francis Group The Lower Cretaceous Ilek Formation in Western Siberia (Russia) has yielded various vertebrate fossils, including skeletal remains of dinosaurs. Here we report on a fragmentary theropod egg from the vertebrate locality Shestakovo 3 of the Ilek Formation in Kemerovo Province. We assign the specimen to the oogenus Prismatoolithus (oofamily Prismatoolithidae) as Prismatoolithus ilekensis oosp. nov., on the basis of the following unique combination of characters: ovoid-shaped egg; thin eggshell 300–330 μm thick; angustiprismatic morphotype; eggshell with three different layers; gradual transition between mammillary layer and prismatic layer; abrupt contact between prismatic layer and external layer; mammillary layer to prismatic layer to external layer thickness ratio is 1:3:0.6; prismatic layer with ill-defined squamatic texture; angusticanaliculate pore system; and smooth outer surface. Like other Early Creataceous Prismatoolithus, the egg of Prismatoolithus ilekensis oosp. nov. was laid by a small bodied theropod dinosaur (troodontid or primitive bird) and this taxonomic attribution is supported by results of our phylogenetic analysis. Prismatoolithus ilekensis oosp. nov. is the first Early Cretaceous ootaxon from Russia. urn:lsid:zoobank.org:act:734EAD40-86C3-488B-A61E-B5FF7378BC0

The nucleus of the Sagittarius dSph galaxy and M54: a window on the process of galaxy nucleation

We present the results of a thorough study of the nucleus of the Sgr dwarf spheroidal galaxy and of the bright globular cluster M54 that resides within the same nucleus (Sgr,N). We have obtained accurate radial velocities and metallicity estimates for 1152 candidate Red Giant Branch stars of Sgr and M54 lying within ~ 9 arcmin from the center of the galaxy, from Keck/DEIMOS and VLT/FLAMES spectra of the infrared Calcium II triplet. Using both velocity and metallicity information we selected two samples of 425 and 321 very-likely members of M54 and of Sgr,N, respectively. The two considered systems display significantly different velocity dispersion profiles: M54 has a steeply decreasing profile from r=0, where sigma= 14.2 km/s, to r=3.5 arcmin where it reaches sigma=5.3 km/s, then it appears to rise again to sigma= 10 km/s at r=7 arcmin. In contrast Sgr,N has a uniformly flat profile at sigma=9.6 km/s over the whole 0 < r < 9 arcmin range. Using data from the literature we show that the velocity dispersion of Sgr remains constant at least out to r ~ 100 arcmin and there is no sign of the transition between the outer flat-luminosity-profile core and the inner nucleus in the velocity profile. These results - together with a re-analysis of the surface brightness profile of Sgr,N and a suite of dedicated N-body simulations - provide very strong support for the hypothesis that the nucleus of Sgr formed independently of M54, which probably plunged to its present position, coincident with Sgr,N, because of significant decay of the original orbit due to dynamical friction.Comment: Accepted for publication by the Astronomical Journal. emulateapj.cls, 26 pag., 21 low resolution figures. A full-resolution color version of the paper can be retrieved from http://www.bo.astro.it/SGR/Sgr_nucleus.ps.g

Эфиромасличные культуры семейства Lamiaceae для вертикального овощеводства

Relevance. Vertical farming – is a new and advanced direction in greenhouse vegetable cultivation. Expansion biodiversity of plants for vertical farming occur with help of green leafy vegetables. Plants of Lamiaceae family are well known as aromatic and medicinal plants with high content of substances with antioxidant activity. It allows use these plants as a base for the functional nutrition. Leafy parts the plants of Lamiaceae family may be used as aromatic and healthy additions to traditional foods, such as salads, soups and sauces.  Goal of the study: analysis the biochemical composition of leafy parts plants of Lamiaceaefamily, cultivated at the multi circle hydroponic construction. Materials and methods. 1) Plants: Monarda fistulosa L. (the breeding sample №5 U.P.), Monarda citriodora Cerv. ex Lag. (Simka variety), Melissa officinalisL. (Zhemchuzhina variety). 2) secondary metabolites: flavonoid glycoside linarozid and steroid glycoside moldstim. Methods. 1) cultivation of plants Lamiaceae family at the 5 circles hydroponic construction; 2) analytic methods: determination of dry matter content, determination of ascorbic acid content, determination sum of chlorophylls and carotenoids, determination sum of antioxidants; 3) statistical methods. Results. First experiment the cultivation of plants Lamiaceae family at the multi circle hydroponic construction was successful. Location of plants at different circles of hydroponic installation didn’t influence the content of dry matter, ascorbic acid and sum of chlorophylls in leafy parts of plants Monarda fistulosaL. The content of dry matter and sum of antioxidants in leafy parts of Monarda fistulosaL. plants of the first cutting is significantly higher than of other cuttings. So, we can recommend the leaves of Monarda fistulosa L. plants of the first cutting for the healthy additions in the scheme of the functional nutrition. Seed treatment with water solutions of secondary metabolites changed significantly the weight of leaves: it was increased in Monarda citriodoraCerv. ex Lag. and in Melissa officinalisL. Актуальность. Вертикальное овощеводство – передовое направление овощеводства защищённого грунта. Расширение биоразнообразия растений для вертикального овощеводства происходит за счёт зеленных культур. Представители семейства Lamiaceae хорошо известны как ароматические и медицинские растения с высоким содержанием антиоксидантов. Это позволяет использовать их как листовые овощи в программах по функциональному питанию. Цель исследований: анализ биохимического состава листовой массы растений семейства Lamiaceae, культивируемых на многоярусной узкостеллажной гидропонике (МУГ). Материалы и методы. Материалы исследований: растения: Monarda fistulosa L. (селекционный образец №5 Ю.П.), Monarda citriodora Cerv. ex Lag. (сорт Симка), Melissa officinalis L. (сорт Жемчужина) и вторичные метаболиты: флавоноидный гликозид линарозид и стероидный гликозид молдстим. Растения семейства Lamiaceae культивировали на пятиярусной гидропонной установке. При проведении исследований использовали аналитические: определение содержания сухого вещества, аскорбиновой кислоты, суммарного содержания хлорофиллов и каротиноидов, суммарного содержания антиоксидантов; и статистические методы: однофакторный дисперсионный анализ (Microsoft Excel, 2003), двухфакторный дисперсионный анализ. Результаты исследований. Первый опыт культивирования растений семейства Lamiaceae на пятиярусной гидропонной установке был успешен. Расположение на разных ярусах конструкции не влияет на содержание сухого вещества, аскорбиновой кислоты и суммарное содержание хлорофиллов в надземной части растений Monarda fistulosa L. Содержание сухого вещества и суммарное содержание антиоксидантов существенно выше в надземной части растений Monarda fistulosa L. первой срезки. Можно рекомендовать листья растений Monarda fistulosaL. первой срезки как полезные добавки в схеме функционального питания. Обработка семян водными растворами вторичных метаболитов может существенно увеличить вес надземной части растений семейства Lamiaceae

ЭФФЕКТИВНОСТЬ ДЕЙСТВИЯ РЕГУЛЯТОРОВ ПРИРОДНОГО ПРОИСХОЖДЕНИЯ ПРИ ВЫРАЩИВАНИИ МОРКОВИ

Utilization of natural biostimulators- secondary metabolits of plants in carrot production was studied. Purified fractions with glycoside content are characterized with extraction and adsorption chromatography from the next plants: linarosides – from Linaria vulgaris Mill. L., melampyrosides - from Melampyrum nemorosum L., genistifoliosides - from Linaria genistifolia L., verbascosides - from Verbascum phlomoides L., melongosides - from Solanum melongena L. and tomatoside – from Solanum lycopersicum L. The former substance was registered under market mark “ecostim”. Prospective preparations for the improvement plant resistance to abiotic and biotic stresses have been identified in laboratory tests. Treatment of carrot seeds with water solutions of such substances accelerates plant growth, increases yield and improves quality of storage roots. We determined regimes of seed treatment (substance concentration, time of exposition) and gave recommends by using of natural regulators in the technologic scheme of carrot cultivation. Using secondary metabolites of higher plants stimulates the energy of germination (on 1,5-6,7%) and increases the field germination of carrot seeds (on 23-40%). It accelerates leaves growth and increases the mass of storage roots, improves biochemical characteristics of storage roots (accumulation the β- carotene and decreasing of nitrates content). Yield of storage roots increases on 4,2-8,5 t/ha, and yield of market quality roots increases on 4-6 t/ha.Изучена возможность использования природных биорегуляторов – вторичных метаболитов высших растений – в качестве элемента технологии при выращивании моркови. Из представителей сем. Solanaceae и Scrophularеaceaе методом экстракции и последующей адсорбционно-распределительной хроматографии получены и химически охарактеризованы очищенные гликозидсодержащие фракции: из Linaria vulgaris Mill. L. – линарозиды, из Melampyrum nemorosum L. - мелампирозиды, из Linaria genistifolia L. – генистифолиозиды, из Verbascum phlomoides L. - вербаскозиды, из Solanum melongena L. мелонгозиды и из Solanum lycopersicum L. разрешенный к применению препарат экостим на основе стероидного гликозида – томатозид. В процессе лабораторного тестирования выявлены наиболее перспективные соеди- нения, способные благодаря предпосевному замачиванию семян в их водных растворах, индуцировать устойчивость растений моркови к абиотическим и биотическим стрессам, что в свою очередь способствует ускорению роста, повышению урожайности и улучшению качества получаемой продукции. Определены оптимальные параметры обработки семян (концентрации биорегуляторов в водных растворах, время экспозиции семян), даны рекомендации по применению предпосевной обработки семян природными биорегуляторами в технологической схеме выращивания моркови. Использование вторичных метаболитов высших растений стимулирует энергию прорастания (на 1,5-6,7%) и повышает полевую всхожесть семян моркови (на 23-40%), обеспечивает дружное появление всходов и оптимальную густоту стояния. Это ускоряет динамику нарастания ассимиляционной поверхности растений и массы корнеплода, улучшает биохимические показатели конечной продукции, способствуя накоплению в них каротина и снижению нитратов. При этом урожайность увеличивается на 4,2-8,5 т/га, выход товарной продукции - на 4-6 т/га.

ПОВЫШЕНИЕ СЕМЕННОЙ ПРОДУКТИВНОСТИ РОДИТЕЛЬСКОЙ ЛИНИИ ГИБРИДА F1 КАПУСТЫ БЕЛОКОЧАННОЙ ПОД ДЕЙСТВИЕМ СТЕРОИДНЫХ ГЛИКОЗИДОВ

Development of F1 cabbage hybrids is concerned to difficulties of artificial pollination, low ability to developovary leading to low seed productivity in parental lines. The aim of the study is to improve the seed productivity of self-pollinated parental line 3/14C for F1 cabbage hybrid ‘Krasotka’. Secondary plant metabolites such as steroid glycosides can help overcome these difficulties and increase the seed productivity (Blandinskaya et al., 2013). 3/14C-line was propagated by backcrossing flowers in bud stage with artificial self-pollination, using water solutions of steroid glycosides ‘Moldstim’ and ‘Melongozid’ (in concentrations 0.1%, 0.01%, 0.001% and 0.0001%). Seed productivity was evaluated according to methods that were elaborated earlier (Bukharov A.F. et al., 2011, 2013). It was established that all parameters of seed formation and development were increased significantly under treatment of steroid glycosides ‘Moldstim’ and ‘Melongozid’ in mentioned concentration. Differences were significant at the 5%-level of significance. Concentration of 0.001% was more effective than other both preparations. These preparations stimulated all phases of seed development. Number of fruits was increased by 16-39%, a number of seeds per one the pod was also increased by 17-38%, mass of 1000 seeds was increased by 9-12%. Thus, the seed productivity of parental line 3/14C F1cabbage hybrid named ‘Krasotka’ was increased by 44 to 105% as result of the action of steroid glycosides. Thus, ‘Moldstim’ and ‘Melongozid’ in concentration of 0.001% can be recommend as physiological stimulators to improve seed productivity in parental lines necessary for development of F1 cabbage hybrids.Получение F1 гибридов капусты белокочанной связано с трудностями принудительного опыления: слабой завязываемостью семян и низкой семенной продуктивностью исходных родительских линий. Цель настоящей работы: повысить семенную продуктивность самонесовместимой родительской линии 3/14С F1 гибрида капусты белокочанной Красотка с использованием вторичных метаболитов растений – стероидных гликозидов. Эксперимент провели в 2013-2015 годах на базе ВНИИ овощеводства. Размножение линии 3/14С осуществляли путем инцухтирования цветков в состоянии бутона, используя в качестве стимулирующих факторов водные растворы стероидных гликозидов молдстима и мелонгозида в концентрациях 0,1%, 0,01%, 0,001% и 0,0001%. Установлено, что стероидные гликозиды молдстим и мелонгозид в исследуемых концентрациях существенно повышают все показатели образования и развития семени. Положительные эффекты препаратов проявились на всех этапах опыления-оплодотворения и развития семени и выразились в существенном повышении всех параметров семенной продуктивности. Различия были существенны на 5%-ном уровне значимости. Наиболее эффективной для обоих препаратов была концентрация 0,001%. Завязываемость плодов выросла на 16-39%, осеменённость плода – на 17-38%, масса 1000 семян – на 9-12%. В результате семенная продуктивность родительской линии3/14С F1 гибрида капусты белокочанной Красотка увеличилась на 44-105%. Следовательно, препараты молдстим и мелонгозид (в концентрации 0,001%) можно рекомендовать как физиологические стимуляторы для повышения семенной продуктивности самонесовместимых линий в процессе получения F1 гибридов капусты белокочанной методом принудительного опыления