Desarrollo larval de Etropus longimanus (Paralichthyidae) y Symphurus trewavasae (Cynoglossidae) en la costa Bonaerense, Argentina

The larval development of Etropus longimanus and Symphurus trewavasae (Pleuronectiformes) off the Buenos Aires coast was described. Both species have an elongated body; however, as the total length of Etropus longimanus larvae increased, their body became deeper. In Symphurus trewavasae the intestine was noticeably coiled. In E. longimanus the notochord flexion started at 3.9 mm and was completed at 5.0 mm standard length (SL). Vertebral formation began in larvae with a 4.6 mm SL and the definitive number of vertebrae (34-39) was observed in larvae of 4.8 mm SL. The dorsal fin had two elongated rays and the pelvic fins had only one. In Symphurus trewavasae the notochord flexion began at 5.9 mm and was completed at 8.0 mm SL. Migration of the right eye was completed in the metamorphic stage at 10.5 mm SL. Vertebral column ossification finished in flexion larvae of 6-7 mm SL, with a total number of 48-50 vertebrae. Four elongated rays of similar length were observed on the dorsal fin.Se describe el desarrollo larvario de Etropus longimanus y Symphurus trewavasae (Pleuronectiformes) que habitan la costa de Buenos Aires. El cuerpo de las larvas de ambas especies es alargado, pero en Etropus longimanus la altura del cuerpo se incrementa con el crecimiento. El tubo digestivo de Symphurus trewavasae es considerablemente enrollado. La flexión del urostilo en larvas de E. longimanus se inició cerca de los 3.9 mm y finalizó cerca de los 5 mm de longitud estándar (LS). Se observaron vértebras definidas en larvas de 4.6 mm LS y el número definitivo (34-39) se visualizó en individuos de 4.8 mm LS. Se distinguen dos radios largos al inicio de la aleta dorsal y uno en la pélvica. En Symphurus trewavasae la flexión del urostilo comenzó cerca de los 5.9 mm LS y finalizó aproximadamente a los 8.0 mm LS. La migración del ojo derecho hacia el lado izquierdo de la cabeza finalizó en el estadio de metamorfosis (10.5 mm LS). La osificación de la columna vertebral se completó en larvas entre los 6 y 7 mm LS, con un número total de 48-59 vértebras. Al inicio de la aleta dorsal se distinguen 4 radios largos con igual longitud

Measured GFR in murine animal models: review on methods, techniques, and procedures

Animal models; Glomerular filtration rate; Kidney damageModels animals; Taxa de filtració glomerular; Dany renalModelos animales; Tasa de filtración glomerular; Daño renalChronic kidney disease (CKD) is one of the most common chronic diseases worldwide, with increasing rates of morbidity and mortality. Thus, early detection is essential to prevent severe adverse events and the progression of kidney disease to an end stage. Glomerular filtration rate (GFR) is the most appropriate index to evaluate renal function in both clinical practice and basic medical research. Several animal models have been developed to understand renal disease induction and progression. Specifically, murine models are useful to study the pathogenesis of renal damage, so a reliable determination of GFR is essential to evaluate the progression of CKD. However, as in clinical practise, the estimation of GFR in murine by levels of serum/urine creatinine or cystatin-C could not be accurate and needed other more reliable methods. As an alternative, the measurement of GFR by the clearance of exogenous markers like inulin, sinistrin, 51Cr-EDTA, 99mTc-DTPA, 125I-iothalamate, or iohexol could be performed. Nevertheless, both approaches—estimation or measurement of GFR—have their limitations and a standard method for the GFR determination has not been defined. Altogether, in this review, we aim to give an overview of the current methods for GFR assessment in murine models, describing each methodology and focusing on their advantages and limitations.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work is supported by funding from the Instituto de Salud Carlos III with the following grants: AERR is a recipient of a contract from the Sara Borrell programme (C21/00142) and STT of PFIS FI20/00147

Very short-term forecast of near-coastal flow using scanning lidars

Wind measurements can reduce the uncertainty in the prediction of wind energy production. Today, commercially available scanning lidars can scan the atmosphere up to several kilometres. Here, we use lidar measurements to forecast near-coastal winds with lead times of 5 min. Using Taylor's frozen turbulence hypothesis together with local topographic corrections, we demonstrate that wind speeds at a downstream position can be forecast by using measurements from a scanning lidar performed upstream in a very short-term horizon. The study covers 10 periods characterised by neutral and stable atmospheric conditions. Our methodology shows smaller forecasting errors than those of the persistence method and the autoregressive integrated moving average (ARIMA) model. We discuss the applicability of this forecasting technique with regards to the characteristics of the lidar trajectories, the site-specific conditions and the atmospheric stability

The fungal subtilase AsES elicits a PTI-like defence response in Arabidopsis thaliana plants independently of its enzymatic activity

Acremonium strictum elicitor subtilisin (AsES) is a 34‐kDa serine‐protease secreted by the strawberry fungal pathogen A. strictum. On AsES perception, a set of defence reactions is induced, both locally and systemically, in a wide variety of plant species and against pathogens of alternative lifestyles. However, it is not clear whether AsES proteolytic activity is required for triggering a defence response or if the protein itself acts as an elicitor. To investigate the necessity of the protease activity to activate the defence response, AsES coding sequences of the wild‐type gene and a mutant on the active site (S226A) were cloned and expressed in Escherichia coli. Our data show that pretreatment of Arabidopsis plants with inactive proteins, i.e. inhibited with phenylmethylsulphonyl fluoride (PMSF) and mutant, resulted in an increased systemic resistance to Botrytis cinerea and expression of defence‐related genes in a temporal manner that mimics the effect already reported for the native AsES protein. The data presented in this study indicate that the defence‐eliciting property exhibited by AsES is not associated with its proteolytic activity. Moreover, the enhanced expression of some immune marker genes, seedling growth inhibition and the involvement of the co‐receptor BAK1 observed in plants treated with AsES suggests that AsES is being recognized as a pathogen‐associated molecular pattern by a leucine‐rich repeat receptor. The understanding of the mechanism of action of AsES will contribute to the development of new breeding strategies to confer durable resistance in plants

Intercultural and digital competence in teacher training from an international perspective: Poland Portugal, Slovakia, Spain and Russia

This article presents one of the studies from the European project IRNET, a network that pursues the development of new tools and methods for advanced pedagogical science in the field of ICT instruments, elearning and intercultural competences. We intend to explore the application of international and national policies of innovative, digital Education and intercultural competences in teacher training, as well as innovative educational practices. Another objective of the project is also to identify such practises and/or research projects in order to foster intercultural and digital competences in Spain, Poland , Slovak, Portugal and Russia. From a comparative perspective and on a qualitative paradigm, we have analyzed three comparative units that have allowed, among other things, us to look at the impact of projects financed with European or national funds as promoters of change and innovation related to intercultural training and technologies information. Finally, it has been observed that teaching trends point to a professorship endowed with skills that integrate intercultural models from the joint search for the school of the future, and for that online networks and virtual learning communities are key resources

Explorando con realidad virtual interactiva

Continuando una línea de investigación y desarrollo que se viene trabajando en el LINTI, Laboratorio de Investigación en Nuevas Tecnologías Informáticas de la UNLP (Argentina), en este artículo se presenta la concreción de los proyectos que utilizan Realidad Aumentada (RA) con fines sociales y el estado de avance de aquellos que utilizan Realidad Virtual (RV) para potenciar aplicaciones móviles. Dentro de esta línea se abordan aspectos relacionados al desarrollo de aplicaciones educativas interactivas y materiales didácticos, así como también una nueva línea relacionada con RV aplicada a la arquitectura. En este artículo, se continúa con las líneas de I+D presentadas en WICC 2021, donde se analizaron y evaluaron entornos de desarrollo para aplicaciones de gamificación usando RA y RV y se presentan los avances en los desarrollos de aplicaciones usando RA y RV.Red de Universidades con Carreras en Informátic

Polycomb Repressive Complex 2 Regulates Genes Necessary for Intestinal Microfold Cell (M Cell) Development

BACKGROUND & AIMS: Microfold cells (M cells) are immunosurveillance epithelial cells located in the Peyer's patches (PPs) in the intestine and are responsible for monitoring and transcytosis of antigens, microorganisms, and pathogens. Mature M cells use the receptor glycoprotein 2 (GP2) to aid in transcytosis. Recent studies have shown transcription factors, Spi-B and SRY-Box Transcription Factor 8 (Sox8). are necessary for M-cell differentiation, but not sufficient. An exhaustive set of factors sufficient for differentiation and development of a mature GP2+ M cell remains elusive. Our aim was to understand the role of polycomb repressive complex 2 (PRC2) as an epigenetic regulator of M-cell development. Estrogen-related-receptor gamma (Esrrg), identified as a PRC2-regulated gene, was studied in depth, in addition to its relationship with Spi-B and Sox8. METHODS: Comparative chromatin immunoprecipitation and global run-on sequencing analysis of mouse intestinal organoids were performed in stem condition, enterocyte conditions, and receptor activator of nuclear factor kappa B ligand-induced M-cell condition. Esrrg, which was identified as one of the PRC2-regulated transcription factors, was studied in wild-type mice and knocked out in intestinal organoids using guide RNA's. Sox8 null mice were used to study Esrrg and its relation to Sox8. RESULTS: chromatin immunoprecipitation and global run-on sequencing analysis showed 12 novel PRC2 regulated transcription factors, PRC2-regulated Esrrg is a novel M-cell-specific transcription factor acting on a receptor activator of nuclear factor kappa B ligand-receptor activator of nuclear factor kappa B-induced nuclear factor-kappa B pathway, upstream of Sox8, and necessary but not sufficient for a mature M-cell marker of Gp2 expression. CONCLUSIONS: PRC2 regulates a significant set of genes in M cells including Esrrg, which is critical for M-cell development and differentiation. Loss of Esrrg led to an immature M-cell phenotype lacking in Sox8 and Gp2 expression. Transcript profiling: the data have been deposited in the NCBI Gene Expression Omnibus database (GSE157629).Peer reviewe

Expanding on the Fundamental Metallicity Relation in Dwarf Galaxies with MUSE

The mass-metallicity relation (MZR) represents one of the most important scaling relations in the context of galaxy evolution, comprising a positive correlation between stellar mass and metallicity (Z). The fundamental metallicity relation (FMR) introduces a new parameter, the star formation rate (SFR), in the dependence. While several studies found that Z is anti-correlated with the SFR at fixed mass, the validity of this statement has been questioned extensively and no widely-accepted consensus has been reached yet. With this work, we investigate the FMR in nine nearby, spatially-resolved, dwarf galaxies, using gas diagnostics on integral-field spectroscopic data of the Multi Unit Spectroscopic Explorer (MUSE), pushing such investigations to lower galaxy masses and higher resolutions. We find that both the MZR and FMR exhibit different behaviours within different star forming regions of the galaxies. We find that the SFR surface density - metallicity anti-correlation is tighter in the low-mass galaxies of our sample. For all the galaxies considered, we find a SFR surface density - stellar mass surface density correlation. We propose that the main reason behind these findings is connected to the accretion mechanisms of the gas fuelling star formation -- low-mass, metal-poor galaxies accrete pristine gas from the intergalactic medium, while in more massive and metal-enriched systems the gas responsible for star formation is recycled from previous star forming episodes.Comment: 15 pages, 8 figures, accepted for publication in Astronomy & Astrophysic

Report on the implementation of WP3 "Analyses and evaluation of the ICT level, e-learning and intercultural development in every participating country" in the framework of the IRNet project

This article, prepared by an international team of authors – researchers from different scientific areas, connected with ICT, e-learning, pedagogy, and other related disciplines – focuses on the objectives and some results of the IRNet international project. In particular, this article describes the research tools, methods and some procedures of the WP3 “Analyses and Evaluation of the ICT Level, E-learning and Intercultural Development in Every Participating Country”: Objectives, Tasks, Deliverables, and implementation of research trips. Except that, the article presents more important events, such as (video)conferences, seminars, workshops, an e-round table debate; among these events are ICTE2014, DLCC2014, “New Educational Strategies in Modern Information Space,” “Hightech information educational environment,” during which some more important results of the project research were presented. The list of publications includes 32 papers and a manuscript with WP3 results. Researchers from Poland, Russia, Ukraine, the Netherlands, Spain, Slovakia, Portugal, Czech Republic and Australia analysed the results of WP3 in the context of the next stages and Work packages of IRNet project – International Research Network