52 research outputs found
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Reversibly binding resin for algal harvest and concentration
The present invention includes a method for harvesting or separating one or more biological cells from an aqueous feed, a stream, a suspension, or any combinations thereof by providing the aqueous feed, the stream, or the suspension comprising the one or more biological cells in a tank or a vessel; providing one or more ion-exchange resins, wherein the ion-exchange resin is an anion-exchange resin; contacting the anion-exchange resin with the aqueous feed; binding the one or more biological cells to the anion-exchange resin; releasing or eluting the bound one or more biological cells by changing the pH; and collecting the released one or more biological cells to obtain a concentrated slurry or suspension of the one or more biological cells.Board of Regents, University of Texas Syste
Murine T-Lymphomas Corresponding to the Immature CD4-8+ Thymocyte Subset
N-methyl-N-nitrosourea induces murine CD4-8+ T-lymphomas that express high levels
of J11d and low levels of CD5 antigens, a phenotype characteristic of immature CD4-8+
thymocytes. This assignment is supported by the fact that CD4-8+ lymphoma cell lines
acquire CD4 expression after intrathymic (i.t.) transfer, a finding consistent with the
established precursor potential of the normal immature CD4-8+ subset. CD4+8+ lymphomas recovered after i.t. transfer maintain a CD4+8+ phenotype in long-term culture. Northern blot analyses reveal that CD4 expression is regulated at the
transcriptional level in immature CD4-8+ and CD4+8+ cell lines. CD4-8+ lymphomas
express low levels of functional CD3/TCR complexes that mediate intracellular Ca2+
mobilization in response to CD3 or α/β-TCR monoclonal antibody. These data suggest
that the immature CD4-8+ subset contains cells capable of undergoing TCR-mediated
signaling and selection events. In contrast to normal immature CD4-8+ cells, which
comprise a heterogeneous and transient subset, the CD4-8+ lymphoma lines provide
stable, monoclonal models of the immature CD4-8+ stage of thymocyte development
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Non-dispersive process for insoluble oil recovery from aqueous slurries
The development and application of a novel non-polar oil recovery process utilizing a non-dispersive solvent extraction method to coalesce and recover oil from a bio-cellular aqueous slurry is described herein. The process could apply to recovery of algal oil from a lysed algae slurry, recovery of Omega fatty acids from a bio-cellular aqueous feed, recovery of Beta-carotene from a bio-cellular aqueous feed and for the removal from produced water in oil production and similar type applications. The technique of the present invention utilizes a microporous hollow fiber (MHF) membrane contactor. The novel non-polar oil recovery process described herein can be coupled to a collecting fluid (a non-polar solvent such as heptane, a biodiesel mixture or the previously extracted oil) that is circulated through the hollow fiber membrane. In cases where the biodiesel mixture or the previously extracted oil is used the solvent recovery step (e.g. distillation) can be eliminated.Board of Regents, University of Texas Syste
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Immobilized resins for algal oil extraction
A reverse phase extraction method for the recovery of triglycerides from aqueous slurries of algae is described herein. The present invention discloses the use of immobilized anion exchange and lipid binding resins. As the algae flows past the resin, triglycerides adhere while the bulk of the algae flows through. The lipids, useful for generating biofuels are then are eluted off the resin. The method of the present invention does not require prior drying of the algae, is inexpensive, and does not destroy the algal biomass which can be used for other purposes.Board of Regents, University of Texas Syste
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