Políticas públicas en la era digital y el impacto en los medios digitales radiales de la Provincia de San Luis, Argentina

Con la llegada de las tecnologías, el ámbito público se extendió más allá de las calles o de los mas media y encontró en los espacios digitales el lugar donde es posible visibilizar temas y miradas de sujetos que no tenían acceso a grandes empresas de comunicación. Desde el advenimiento a la democracia, los gobiernos argentinos impulsaron reformas para regular la radiodifusión y políticas de comunicación; con las nuevas tecnologías, lo digital ha constituido un tópico a trabajar y a tener en cuenta por el gobierno nacional y en este caso, por el de la Provincia de San Luis. En el ámbito académico, conocer los medios de comunicación de masas era una necesidad que a raíz de las nuevas tecnologías fue mutando. Es por ello y enmarcado en los objetivos del proyecto, se presentan primeros resultados que incluyen: mapa de medios digitales radiales, políticas públicas vigentes desde el 1/1/2012 hasta el 31/12/2014, en relación a las nuevas tecnologías de la información y la comunicación (TIC) y finalmente una aproximación estadística sobre la relación políticas públicas y medios digitales radiales de la Provincia de San Luis.Fil: Maldonado, Ana Silvina. Universidad Nacional de San LuisFil: Palacios, Ayelén Josefina. Universidad Nacional de San LuisFil: Oro, Pablo Martin. Universidad Nacional de San Lui

Clonogenicity, gene expression and phenotype during neutrophil versus erythroid differentiation of cytokine-stimulated CD34 human marrow cells in vitro.

With the objective to correlate clonogenicity, gene expression and phenotype during differentiation, human bone marrow CD34+ cells were cultured in vitro to stimulate erythroid or neutrophil development, and sorted into five subpopulations according to their surface expression of CD15/CD33 and blood group antigen A/CD117 respectively. Sorted cells were cultured in methylcellulose and analysed by real-time reverse transcription polymerase chain reaction for expression of neutrophil and erythroid marker genes. Surface expression of CD15 coincided with restriction to neutrophil/monocyte differentiation and A antigen with restriction to erythroid differentiation. GATA-2 mRNA was down-regulated during both neutrophil and erythroid maturation, whereas GATA-1, SCL, ABO, erythropoietin receptor, Kell, glycophorin A, β-globin and α-haemoglobin stabilizing protein were up-regulated during erythroid differentiation and silenced during neutrophil differentiation. CCAAT/enhancer-binding protein (C/EBP)-α, PU.1, granulocyte colony-stimulating factor receptor, PR3, C/EBP-e and lactoferrin were sequentially expressed during neutrophil differentiation but rapidly down-regulated during the early erythroid stages. Nuclear factor erythroid-derived 2 (NF-E2) and glycophorin C were expressed both during neutrophil and erythroid differentiation. Our data support the notion of early expression of several lineage-associated genes prior to actual lineage commitment, defined by surface expression of CD15 and A antigen as markers for definitive neutrophil/monocyte and erythroid differentiation respectively. Previous findings, primarily from cell lines and mouse models, have been extended to adult human haematopoiesis

Crystal structure dependence of the energy transfer from tb(III) to yb(III) in metal–organic frameworks based in bispyrazolylpyridines

Luminescent mixed lanthanide metal−organic framwork (MOF) materials have been prepared from two polyheterocyclic diacid ligands, 2,6-bis(3-carboxy-1-pyrazolyl)pyridine and 2,6- bis(4-carboxy-1-pyrazolyl)pyridine. The crystal structures of the two organic molecules are presented together with the structures for the MOFs obtained by hydrothermal synthesis either with Yb(III) or mixed Tb(III)/Yb(III) ions. Different coordination architectures result from each ligand, revealing also important differences between the lanthanides. The mixed lanthanide metal−organic frameworks also present diverse luminescent behavior; in the case of 2,6-bis(4-carboxy-1- pyrazolyl)pyridine, where no coordinated water is present in the metal environment, Tb(III) and Yb(III) characteristic emission is observed by excitation of the bispyrazolylpyridine chromophoreThe research leading to these results has received funding from ERCROS S.A. (Aranjuez, Spain

Draft genome sequence of Lactobacillus helveticus ATCC 12046

Lactobacillus helveticus is a lactic acid bacterium used traditionally in the dairy industry, especially in the manufacture of cheeses. We present here the 2,141,841-bp draft genome sequence of L. helveticus strain ATCC 12046, a potential starter strain for improving cheese production.Fil: Ruzal, Sandra Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Palomino, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fina Martin, Joaquina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fernández Do Porto, Darío Augusto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Formación e Investigación en Enseñanza de las Ciencias; Argentin

Knowledge And Attitudes Of Latin American Gynecologists Regarding Unplanned Pregnancy And Use Of Combined Oral Contraceptives.

Unintended pregnancy is a public health problem and unmet medical need worldwide. It is estimated that in the year 2012, almost 213 million pregnancies occurred, and the global pregnancy rate decreased only slightly from 2008 to 2012. It was also estimated that 85 million pregnancies (40% of all pregnancies) were unintended and that 38% ended in an unintended birth. To assess knowledge and attitudes of Latin American (LA) obstetricians and gynecologists (OBGYNs) regarding unintended pregnancies and aspects of combined oral contraceptive (COC) use. A survey was conducted during a scientific meeting about contraception in 2014, in which OBGYNs from 12 LA countries who provide attention in contraception were invited to respond to a multiple-choice questionnaire to assess their knowledge and attitudes regarding unplanned pregnancy and some aspects regarding COC use. A total of 210 OBGYNs participated in the study. Their knowledge regarding COC failure was low. The participants reported they believed that their patients habitually forgot to take a pill and that their patients did not know what to do in these situations. They were aware of the benefits of COC use; however, they were less prone to prescribe COCs for the purpose of protecting against ovarian and endometrial cancer, and one-quarter of them had doubts about the association between COC use and cancer risk. The interviewed LA OBGYNs showed some flaws in terms of knowledge of COC failure rates and the non-contraceptive benefits and risks of COCs. To adequately counsel their patients regarding COC intake, OBGYNs must be updated regarding all aspects of COC use.7485-49

Respuesta del tabaco al riego deficitario para dos fechas de trasplante

En Argentina la producción de tabaco Virginia se localiza en Jujuy y Salta, donde la reposición de agua al cultivo se realiza principalmente con riego gravitacional. La posibilidad de regar con mayor precisión y la poca sensibilidad del cultivo a un estrés moderado permite suponer que es factible regar el tabaco de manera deficitaria. Por lo que, el objetivo de este trabajo fue evaluar la respuesta de la materia seca de hoja de tabaco y sus componentes al riego deficitario de alta frecuencia durante la fase previa a las precipitaciones en el Valle de Lerma (Cerrillos, Salta) para dos fechas de trasplante, determinando, además, atributos relacionados con la eficiencia en el uso de la radiación y del agua. El estudio se realizó en la EEA del INTA Salta durante tres ciclos agrícolas (2013/14, 2015/16 y 2016/17). Se trabajó con tabaco Virginia variedad K394. Los tratamientos consistieron en la combinación de dos factores, momento de trasplante (Te: temprano y Ta: tardío) y necesidad de riego (NR) con niveles de reposición del 50 y 100%. En diferentes momentos se determinó materia seca de hoja (MSh), materia seca aérea total (hoja+tallo) (MSa) e índice de área foliar (IAF) y masa foliar específica (MFE). En 2015/16 y 2016/17 se determinó filocrono, radiación interceptada acumulada por el cultivo (Rint) y eficiencia de uso de radiación (EUR). Se calculó la eficiencia de uso del agua considerando el agua de riego (EUAr) y el agua aportada por las precipitaciones y el riego (EUApr) a cosecha. La MSh a cosecha fue menor en 2013/14 que en los otros dos ciclos agrícolas, pero fue similar para las dos fechas de trasplante en cada ciclo agrícola. Si bien Te recibió entre 300 y 400 MJ de radiación incidente más que Ta, estos solo se corresponden con un 3% extra de Rint acumulada durante el ciclo agrícola. Por lo tanto, la menor MSh en 2013/14 se explicó por el menor IAF. En 2013/14 y 2016/17 el IAF y la MFE fueron similares entre fechas de trasplante, mientras que en 2015/16 el menor IAF a cosecha de Te generó hojas con mayor MFE. La EUR a cosecha varió entre los 0,46 y 0,48 g/MJ sin diferenciarse entre fechas de trasplante. En 2015/16 y 2016/17, donde el fertilizante utilizado fue líquido, reponer el 50% de la NR no penalizó la MSh y el IAF, lo que sí ocurrió en 2013/14 (donde se utilizó fertilizante sólido). La EUApr fue menor en 2013/14 que en los otros ciclos agrícolas; siempre fue mayor en Ta y solo mayor en 50% de NR en 2015/16, mientras que la EUAr fue en todos los ciclos agrícolas mayor con la combinación de Ta y 50% de NR. Se concluye que el riego deficitario controlado puede ser una alternativa viable para el cultivo de tabaco en el Valle de Lerma, sin embargo, deberían profundizarse estudios donde se considere la calidad de hoja obtenida.The production of tobacco Virginia in Argentina is located at Jujuy and Salta where crop irrigation is mainly done by a gravity system. The possibility of using precision irrigation methods and the low sensitivity of the crop to moderate water stress allow assuming that it is feasible to irrigate tobacco with a deficit strategy. The aim of this study was to evaluate the effects of high frequency deficit irrigation during the period that precede summer precipitation at the Valle de Lerma (Cerrillos, Salta) on tobacco leaf dry matter and its main component for two transplant dates. Attributes related to water and radiation use efficiencies were also determined. The study was conducted during three growing cycles (2013/14, 2015/16 y 2016/17) at INTA EEA-Salta with the tobacco Virginia variety K394. Treatments consisted of the combination of two transplant dates (early, Te; late, Ta) and two level of irrigation requirement (NR): 100 and 50% of NR. Leaf dry matter (MSh), total dry matter (leaf+stem), leaf area index (IAF) and specific foliar mass (MFE) were determined at different moments. Phyllochron, crop accumulated intercepted radiation (Rint) and radiation use efficiency (EUR) were determined in 2015/16 and 2016/17. Water use efficiency was calculated for the 20-24 leaf and harvest samplings considering the irrigation water (EUAr) and the water contributed by the rainfall and irrigation (EUApr). MSh at harvest was lower in 2013/14 than in the other growing cycles, but was similar for the two transplant dates in each growing cycle. Although Te received more incident radiation (300-400 MJ) than Ta, this only represented an extra 3% of Rint during the growing cycle. Therefore, the lowest MSh in 2013/14 was explained by the lowest IAF. IAF and MFE were similar between transplant dates in 2013/14 and 2016/17, whereas for the 2015/16 growing cycle the lower IAF at harvest found for Te generated leaves with higher MFE. There were not differences between transplant date for EUR at harvest, ranging from 0,46 to 0,48 g/MJ. Replacement of 50% of NR did not penalize MSh and IAF during the 2015/16 and 2016/17 growing cycles (when the fertilized used was liquid), whereas it decreased MSh and IAF in 2013/14 (when liquid fertilizer was used). EUApr was lower in 2013/14 than in the other growing cycles. EUApr was always higher for Ta than Te, and for 50% of NR only in 2015/16. During the three growing cycles the lowest EUAr was found with the combination of Ta and 50% of NR. The controlled deficit irrigation is an alternative for tobacco production in Valle de Lerma; however, studies considering leaf quality should be considering.EEA SaltaFil: Diez, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Acreche, Martin Moises. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Body Mass Index in young women and risk of cardiomyopathy: a long-term follow-up study in Sweden

Incidence rates of cardiomyopathies, which are a common cause of heart failure in young people, have increased during the last decades. An association between body weight in adolescence and future cardiomyopathy among men was recently identified. Whether or not this holds true also for women is unknown. The aim was therefore to determine whether for young women being overweight or obese is associated with a higher risk of developing cardiomyopathy. This was a registry-based national prospective cohort study with data collected from the Swedish Medical Birth Register, 1982 to 2014, with up to 33 years of follow-up. Included women were of childbearing age (18-45 years) during the initial antenatal visit in their first or second pregnancy (n=1 393 346). We obtained baseline data on body mass index (BMI), smoking, education, and previous disorders. After exclusions, mainly because of previous disorders, the final sample was composed of 1 388 571 women. Cardiomyopathy cases were identified by linking the Medical Birth Register to the National Patient and Cause of Death registers. In total, we identified 1699 cases of cardiomyopathy (mean age at diagnosis, 46.2 [SD 9.1] years) during the follow-up with an incidence rate of 5.9 per 100 000 observation years. Of these, 481 were diagnosed with dilated cardiomyopathy, 246 had hypertrophic cardiomyopathy, 61 had alcohol/drug-induced cardiomyopathy, and 509 had other forms. The lowest risk for being diagnosed with a cardiomyopathy was detected at a BMI of 21 kg/m , with a gradual increase in risk with higher BMI, particularly for dilated cardiomyopathy, where a hazard ratio of 4.71 (95% CI, 2.81-7.89) was found for severely obese subjects (BMI ≥35 kg/m ), as compared with BMI 20 to <22.5. Elevated BMI among young women was associated with an increased risk of being diagnosed with a subsequent cardiomyopathy, especially dilated cardiomyopathy, starting already at mildly elevated body weight, whereas severe obesity entailed an almost 5-fold increase in risk. With the increasing numbers of persons who are overweight or obese, higher rates of cardiomyopathy can be expected in the future, along with an altered disease burden related to adiposity

Mutações em JAK2, MPL e CALR em neoplasias mieloproliferativas: análise de dissociação em alta resolução

La policitemia vera (PV), la trombocitemia esencial (TE) y la mielofibrosisidiopática (MI) constituyen las Neoplasias Mieloproliferativas cromosomaFiladelfia negativas (NMP Ph-neg). La mutación V617F en el exón 14 delgen JAK2 ha sido descripta en un 90% de los casos de PV y en un 50%de TE y MI. Recientemente, se identificaron mutaciones en el exón 10del gen MPL y en el exón 9 del gen CALR, presentes en un 5 y 73% depacientes con TE y MI sin mutaciones en JAK2, respectivamente. En el presentetrabajo se estudió la detección de dichas mutaciones en 52 pacientescon NMP, mediante amplificaciones por PCR en Tiempo Real con posterioranálisis por High Resolution Melting (HRM) y secuenciación. La mutaciónV617F en JAK2 fue registrada en un 83,3% de pacientes con PV y 42,8%con TE y MI. Un 6,25% y 56,25% de pacientes con TE y MI JAK2 negativosresultaron positivos para mutaciones en el exón 10 de gen del receptorde la trombopoyetina (MPL) y el exón 9 de gen de la calreticulina (CALR).El análisis por HRM puede ser considerado como herramienta diagnósticaeficaz para las NMP debido a su alta sensibilidad, bajo costo y tiempo deprocesado, teniendo en cuenta el impacto clínico que podría tener en los pacientes la detección temprana de dichas mutaciones.Polycythemia vera (PV), essential thrombocythemia (TE) and idiopathic myelofibrosis (MI) are Philadelphia chromosome–negative myeloproliferative neoplasms (MPN-Ph. Neg). The presence of the V617F mutation in exon 14 of the JAK2 gene has been described in 90% of cases of PV and 50% of MI and TE. Recently, mutations in exon 10 of the MPL gene and exon 9 of CALR gene have been identified, which are present in 5 to 73% of patients with TE and MI without mutations in JAK2, respectively. In this work, the detection of these mutations was studied in 52 patients with NMP, using real time PCR amplifications with subsequent High Resolution Melting (HRM) analysis and sequencing. A total of 83.3% of patients with PV and 42.8% with MI and TE were recorded as positive for the V617F mutation in JAK2. A total of 6.25% and 56.25% of the patients with MI and TE with non-mutated JAK2 were positive for mutations in MPL exon 10 and CALR exon 9. HRM analysis could be considered an effective diagnostic tool for NMP due to its high sensitivity, low cost and processing time, taking into account the clinical impact that early detection of such mutations could have on patients.Policitemia vera (PV), trombocitemia essencial (TE) e mielofibrose idiopática (MI) constituem as Neoplasias Mieloproliferativas cromossomo Filadélfia negativas (NMP Ph-neg). A mutação V617F no exon 14 do gene JAK2 foi descrita em 90% dos casos de PV e em 50% de TE e MI. Recentemente, foram identificadas mutações no exon 10 do gene MPL e no exon 9 do gene CALR, presentes em 5 a 73% de pacientes com TE e MI sem mutações em JAK2, respectivamente. Neste trabalho foi estudada a detecção de tais mutações em 52 pacientes com NMP, usando amplificações por PCR em Tempo Real, com posterior análise por High Resolution Melting (HRM) e sequenciamento. 83,3% dos pacientes com PV e 42,8% com TE e MI foram positivos para a mutação V617F em JAK2. 6,25% e 56,25% de pacientes com TE e MI JAK2 negativo foram positivos para mutações no exon 10 de gene do receptor da trombopoietina (MPL) e o exon 9 de gene da calreticulina (CALR). A análise por HRM pode ser considerada como ferramenta de diagnóstico eficaz para as NMP, devido à sua alta sensibilidade, baixo custo e tempo de processamento, tendo em conta o impacto clínico que poderia ter a detecção precoce de tais mutações nos pacientes.Fil: Videla, Yanina Paola. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Fares Taie Instituto de Análisis; ArgentinaFil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fares Taie Instituto de Análisis; ArgentinaFil: Perez Maturo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Fares Taie Instituto de Análisis; ArgentinaFil: Di Gerónimo, Vanesa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Martin , Nazarena. Clínica Colón; ArgentinaFil: Pagani, Fernando. Clínica Colón; Argentin

A Novel Typing Method for Streptococcus pneumoniae Using Selected Surface Proteins

The diverse pneumococcal diseases are associated with different pneumococcal lineages, or clonal complexes. Nevertheless, intra-clonal genomic variability, which influences pathogenicity, has been reported for surface virulence factors. These factors constitute the communication interface between the pathogen and its host and their corresponding genes are subjected to strong selective pressures affecting functionality and immunogenicity. First, the presence and allelic dispersion of 97 outer protein families were screened in 19 complete pneumococcal genomes. Seventeen families were deemed variable and were then examined in 216 draft genomes. This procedure allowed the generation of binary vectors with 17 positions and the classification of strains into surfotypes. They represent the outer protein subsets with the highest inter-strain discriminative power. A total of 116 non-redundant surfotypes were identified. Those sharing a critical number of common protein features were hierarchically clustered into 18 surfogroups. Most clonal complexes with comparable epidemiological characteristics belonged to the same or similar surfogroups. However, the very large CC156 clonal complex was dispersed over several surfogroups. In order to establish a relationship between surfogroup and pathogenicity, the surfotypes of 95 clinical isolates with different serogroup/serotype combinations were analyzed. We found a significant correlation between surfogroup and type of pathogenic behavior (primary invasive, opportunistic invasive, and non-invasive). We conclude that the virulent behavior of S. pneumoniae is related to the activity of collections of, rather than individual, surface virulence factors. Since surfotypes evolve faster than MLSTs and directly reflect virulence potential, this novel typing protocol is appropriate for the identification of emerging clones.This work was supported by a Miguel Servet contract from the Spanish Ministry of Health to AM, Plan Nacional de I+D+I of the Ministry of Science and Innovation (BIO2011-25343, BIO2014-555462-R, SAF2012-39444-C02), Fondo de Investigaciones Sanitarias de la Seguridad Social (PI11/00763) and Fondo Europeo de Desarrollo Regional (FEDER). CIBER Enfermedades Respiratorias is an initiative of the Instituto de Salud Carlos III.Peer reviewedPeer Reviewe

Propuesta de plan museológico para el museo de artes y tradiciones populares “Luis Repetto Málaga”

La presente tesis desarrolla la Propuesta de un Plan Museológico para el Museo de Artes y Tradiciones Populares “Luis Repetto Málaga” del Instituto Riva-Agüero de la Pontificia Universidad Católica del Perú, cuyo objetivo principal es orientar y ordenar su gestión integral. Para ello, se utilizó como herramienta metodológica la publicación española Criterios para la elaboración del plan museológico (2006). Como primer paso, se elaboró un diagnóstico del estado actual del museo en los siguientes ámbitos: institucional, colecciones, arquitectura, exposiciones, difusión y comunicación, seguridad, recursos humanos y recursos económicos, a fin de detectar y priorizar los puntos críticos en cada uno de éstos. Luego del análisis del diagnóstico, se realizó la propuesta de una nueva visión, misión, objetivos y mandato para el museo, para posteriormente, elaborar los programas para cada uno de los ámbitos antes mencionados, con la finalidad de trazar lineamientos ordenadores en cada uno de éstos. Finalmente, se identificaron los proyectos prioritarios en los ámbitos: institucional, colecciones, arquitectura, exposiciones, difusión y comunicación, seguridad, recursos humanos y recursos económicos, que, a nuestro criterio, serían las primeras acciones que el museo debe ejecutar para alcanzar sus objetivos