Lactobacillus helveticus is a lactic acid bacterium used traditionally in the dairy industry, especially in the manufacture of cheeses. We present here the 2,141,841-bp draft genome sequence of L. helveticus strain ATCC 12046, a potential starter strain for improving cheese production.Fil: Ruzal, Sandra Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Palomino, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fina Martin, Joaquina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fernández Do Porto, Darío Augusto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Formación e Investigación en Enseñanza de las Ciencias; Argentin

Allievi, Mariana Caludia

Burguener, Germán Federico

Campos, Josefina

Fernández Do Porto, Darío Augusto

Fina Martin, Joaquina

Palomino, Maria Mercedes

Prado Acosta, Mariano

Ruzal, Sandra Mónica

Genome Announcements

English

LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas

Draft Genome Sequence of Lactobacillus helveticus ATCC12046María Mercedes Palomino,a Germán F. Burguener,b Josefina Campos,c Mariana Allievi,a Joaquina Fina-Martin,aMariano Prado Acosta,a Darío A. Fernández Do Porto,a,b Sandra M. RuzalaaDepartamento de Química Biológica, Pabellón 2, Ciudad Universitaria, Facultad de Ciencias Exactas yNaturales, UBA, IQUIBICEN/CONICET, Buenos Aires, ArgentinabInstituto de Cálculo, Pabellón 2, Ciudad Universitaria, Facultad de Ciencias Exactas y Naturales, UBA, BuenosAires, ArgentinacInstituto Dr. Carlos G. Malbrán, Buenos Aires, ArgentinaABSTRACT Lactobacillus helveticus is a lactic acid bacterium used traditionally inthe dairy industry, especially in the manufacture of cheeses. We present here the2,141,841-bp draft genome sequence of L. helveticus strain ATCC 12046, a potentialstarter strain for improving cheese production.Lactobacillus helveticus is an industrial thermophilic starter or adjunct culture, pre-dominantly used in the fermentation of milk and the manufacture of cheeses (1).Some studies have underlined the health-promoting potential of many strains of L.helveticus, mediated by surface protein from their cell envelope (2–6).L. helveticus ATCC 12046 was obtained from the Centro de Referencia para Lacto-bacilos (CERELA, Tucumán, Argentina). The genome sequence was obtained using awhole-genome shotgun strategy with Illumina MiSeq technology. The number ofpaired-end reads was approximately 402,173 in pairs of 251 bp, totaling 192 Mb ofsequence data. De novo assembly of the contig was done using SPAdes version 3.9.0 (7)and SSPACE version 3.0 (8) and later by means of the reference genome to reducefragmentation. This assembly generated 64 scaffolds, with an N50 value of 89,074 bpand an L50 value of 9 bp. After de novo assembly, contigs were sorted againstL. helveticus CNRZ 32 since 92.63% of the sequenced reads were mapped to its genome(with 93.7% coverage). The draft genome is 2,141,841 bp in length. The GC contentis 36.5 mol%. Genome annotation was done using the standard operating proceduresfrom our own prokaryotic annotation pipeline based on Glimmer for open readingframe prediction (9). Functional annotation included protein function, Gene Ontology(GO) and Clusters of Orthologous Groups (COG) terms, Enzyme Commission (EC)numbers, and Pfam database domains. A total of 2,305 coding sequences (CDSs) and 66structural RNAs (61 tRNAs) were predicted; 582 CDSs (25%) were classified as hypo-thetical or uncharacterized proteins. The Rapid Annotations using Subsystems Tech-nology (RAST) server (10) was used for subsystem descriptions. According to RAST, theannotation assigned 923 CDSs (40%) to RAST subsystems.In common with L. helveticus DPC 4571, the genome presents a large number ofinsertion sequences (ISs) (11). It includes 201 mobile element proteins with highsimilarity to transposase enzyme genes, indicating that they may belong to IS elements,which have been shown to specifically contribute to niche adaptation by promoting avariety of genetic rearrangements. In comparison with CNRZ 32, the genome presentsfour deleted regions ranging from 10 to 40 kb in length. Major gene loss that occurredamong members of the order Lactobacillales indicates early adaptation to nutritionallyrich environments (12). One deletion of interest within contig 11, which mapped withinthe 448212 to 476326 region of CNRZ 32, shows the lack of a cell wall rhamnoseReceived 4 January 2018 Accepted 12January 2018 Published 15 February 2018Citation Palomino MM, Burguener GF, CamposJ, Allievi M, Fina-Martin J, Prado Acosta M,Fernández Do Porto DA, Ruzal SM. 2018. Draftgenome sequence of Lactobacillus helveticusATCC 12046. Genome Announc 6:e01595-17.https://doi.org/10.1128/genomeA.01595-17.Copyright © 2018 Palomino et al. This is anopen-access article distributed under the termsof the Creative Commons Attribution 4.0International license.Address correspondence to Darío A. FernándezDo Porto, dariofd@gmail.com, or Sandra M.Ruzal, sandra@qb.fcen.uba.ar.D.A.F.D.P. and S.M.R. contributed equally to thiswork.PROKARYOTEScrossmVolume 6 Issue 7 e01595-17 genomea.asm.org 1 on February 27, 2018 by guesthttp://genomea.asm.org/Downloaded from containing a polysaccharide cluster. Additionally, in the same deletion we noticed thelack of a glicosyltransferase and an alpha-D-GlcNac-alpha-1,2-L-rhamnosyltransferase,both possibly involved in the side chain formation of rhamnose-glucose cell wallpolysaccharides. The lack of these genes would probably produce variability in thecell wall polysaccharide architecture. It was found that changes in autolytic propertiesobserved in different strains of L. helveticus were due to differences in the chemicalstructure of their cell wall polysaccharides (13). Autolysis of the starter’s strains, as thosebelonging to the L. helveticus species, remains of considerable interest regarding theiruse in dairy fermentation, particularly in the manufacture of cheeses.Accession number(s). This whole-genome shotgun project has been deposited atDDBJ/EMBL/GenBank under the accession number PJRL00000000. The version de-scribed in this paper is version PJRL01000000.ACKNOWLEDGMENTSThis work was supported by grants from the Universidad de Buenos Aires(20020130100444BA and PIP 11220130100162CO) from CONICET (Consejo Nacional deInvestigaciones Científicas y Técnicas) to S.M.R. and by grants from the AgenciaNacional de Promoción Científica y Tecnológica (ANPCyT PICT-2015-1863) to D.A.F.D.P.M.M.P., M.A., M.P.A., D.A.F.D.P., and S.M.R. are members of CONICET.REFERENCES1. Taverniti V, Guglielmetti S. 2012. Health-promoting properties of Lacto-bacillus helveticus. Front Microbiol 3:392. https://doi.org/10.3389/fmicb.2012.00392.2. Johnson-Henry KC, Hagen KE, Gordonpour M, Tompkins TA, ShermanPM. 2007. Surface-layer protein extracts from Lactobacillus helveticusinhibit enterohaemorrhagic Escherichia coli O157:H7 adhesion to epithe-lial cells. Cell Microbiol 9:356 –367. https://doi.org/10.1111/j.1462-5822.2006.00791.x.3. Taverniti V, Stuknyte M, Minuzzo M, Arioli S, De Noni I, Scabiosi C,Cordova ZM, Junttila I, Hämäläinen S, Turpeinen H, Mora D, Karp M, PesuM, Guglielmetti S. 2013. S-layer protein mediates the stimulatory effectof Lactobacillus helveticus MIMLh5 on innate immunity. Appl EnvironMicrobiol 79:1221–1231. https://doi.org/10.1128/AEM.03056-12.4. Meng J, Zhu X, Gao SM, Zhang QX, Sun Z, Lu RR. 2014. Characterizationof surface layer proteins and its role in probiotic properties of threeLactobacillus strains. Int J Biol Macromol 65:110 –114. https://doi.org/10.1016/j.ijbiomac.2014.01.024.5. Skrzypczak K, Gustaw W, Waśko A. 2015. Health-promoting propertiesexhibited by Lactobacillus helveticus strains. Acta Biochim Pol 62:713–720. https://doi.org/10.18388/abp.2015_1116.6. Prado Acosta M, Ruzal SM, Cordo SM. 2016. S-layer proteins fromLactobacillus sp. inhibit bacterial infection by blockage of DC-SIGNcell receptor. Int J Biol Macromol 92:998 –1005. https://doi.org/10.1016/j.ijbiomac.2016.07.096.7. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS,Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV,Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA. 2012. SPAdes: a newgenome assembly algorithm and its applications to single-cell sequenc-ing. J Comput Biol 19:455– 477. https://doi.org/10.1089/cmb.2012.0021.8. Boetzer M, Henkel CV, Jansen HJ, Butler D, Pirovano W. 2011. Scaffoldingpre-assembled contigs using SSPACE. Bioinformatics 27:578–579. https://doi.org/10.1093/bioinformatics/btq683.9. Sosa EJ, Burguener G, Lanzarotti E, Defelipe L, Radusky L, Pardo AM,Marti M, Turjanski AG, Do Porto DF. 2018. Target-Pathogen: a structuralbioinformatic approach to prioritize drug targets in pathogens. NucleicAcids Res 46:D413–D418. https://doi.org/10.1093/nar/gkx1015.10. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K,Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL,Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD,Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O. 2008.The RAST server: Rapid Annotations using Subsystems Technology. BMCGenomics 9:75. https://doi.org/10.1186/1471-2164-9-75.11. Callanan M, Kaleta P, O’Callaghan J, O’Sullivan O, Jordan K, McAuliffe O,Sangrador-Vegas A, Slattery L, Fitzgerald GF, Beresford T, Ross RP. 2008.Genome sequence of Lactobacillus helveticus, an organism distinguishedby selective gene loss and insertion sequence element expansion. JBacteriol 190:727–735. https://doi.org/10.1128/JB.01295-07.12. Makarova KS, Slesarev A, Wolf Y, Sorokin A, Mirkin B, Koonin EV, PavlovA, Pavlova N, Karamychev V, Polouchine N, Shakhova V, Grigoriev I, LouY, Rohksar D, Lucas S, Huang K, Goodstein DM, Hawkins T, PlengvidhyaV, Welker DL, Hughes JE, Goh Y, Benson A, Baldwin KA, Lee JH, Díaz-Muñiz I, Dosti B, Smeianov VV, Wechter W, Barabote R, Lorca GL,Altermann E, Barrangou R, Ganesan B, Xie Y, Rawsthorne H, Tamir D,Parker C, Breidt F, Broadbent JR, Hutkins R, O’Sullivan D, Steele JL, UnluG, Saier MH, Klaenhammer TR, Richardson P, Kozyavkin S, Weimer BC,Mills DA. 2006. Comparative genomics of the lactic acid bacteria. ProcNatl Acad Sci U S A 103:15611–15616. https://doi.org/10.1073/pnas.0607117103.13. Vinogradov E, Valence F, Maes E, Jebava I, Chuat V, Lortal S, Grard T,Guerardel Y, Sadovskaya I. 2013. Structural studies of the cell wallpolysaccharides from three strains of Lactobacillus helveticus with differ-ent autolytic properties: DPC4571, BROI, and LH1. Carbohydr Res 379:7–12. https://doi.org/10.1016/j.carres.2013.05.020.Palomino et al.Volume 6 Issue 7 e01595-17 genomea.asm.org 2 on February 27, 2018 by guesthttp://genomea.asm.org/Downloaded from 

Draft genome sequence of Lactobacillus helveticus ATCC 12046

ABSTRACT
          
            Lactobacillus helveticus
            is a lactic acid bacterium used traditionally in the dairy industry, especially in the manufacture of cheeses. We present here the 2,141,841-bp draft genome sequence of
            L. helveticus
            strain ATCC 12046, a potential starter strain for improving cheese production.
          </jats:p

María Mercedes Palomino

Germán F. Burguener

Josefina Campos

Mariana Allievi

Joaquina Fina-Martin

Mariano Prado Acosta

Darío A. Fernández Do Porto

Sandra M. Ruzal

Crossref

Draft Genome Sequence of
            <i>Lactobacillus helveticus</i>
            ATCC 12046

CONICET Digital

Draft Genome Sequence of Lactobacillus helveticus ATCC12046María Mercedes Palomino,a Germán F. Burguener,b Joseﬁna Campos,c Mariana Allievi,a Joaquina Fina-Martin,aMariano Prado Acosta,a Darío A. Fernández Do Porto,a,b Sandra M. RuzalaaDepartamento de Química Biológica, Pabellón 2, Ciudad Universitaria, Facultad de Ciencias Exactas yNaturales, UBA, IQUIBICEN/CONICET, Buenos Aires, ArgentinabInstituto de Cálculo, Pabellón 2, Ciudad Universitaria, Facultad de Ciencias Exactas y Naturales, UBA, BuenosAires, ArgentinacInstituto Dr. Carlos G. Malbrán, Buenos Aires, ArgentinaABSTRACT Lactobacillus helveticus is a lactic acid bacterium used traditionally inthe dairy industry, especially in the manufacture of cheeses. We present here the2,141,841-bp draft genome sequence of L. helveticus strain ATCC 12046, a potentialstarter strain for improving cheese production.Lactobacillus helveticus is an industrial thermophilic starter or adjunct culture, pre-dominantly used in the fermentation of milk and the manufacture of cheeses (1).Some studies have underlined the health-promoting potential of many strains of L.helveticus, mediated by surface protein from their cell envelope (2–6).L. helveticus ATCC 12046 was obtained from the Centro de Referencia para Lacto-bacilos (CERELA, Tucumán, Argentina). The genome sequence was obtained using awhole-genome shotgun strategy with Illumina MiSeq technology. The number ofpaired-end reads was approximately 402,173 in pairs of 251 bp, totaling 192 Mb ofsequence data. De novo assembly of the contig was done using SPAdes version 3.9.0 (7)and SSPACE version 3.0 (8) and later by means of the reference genome to reducefragmentation. This assembly generated 64 scaffolds, with an N50 value of 89,074 bpand an L50 value of 9 bp. After de novo assembly, contigs were sorted againstL. helveticus CNRZ 32 since 92.63% of the sequenced reads were mapped to its genome(with 93.7% coverage). The draft genome is 2,141,841 bp in length. The GC contentis 36.5 mol%. Genome annotation was done using the standard operating proceduresfrom our own prokaryotic annotation pipeline based on Glimmer for open readingframe prediction (9). Functional annotation included protein function, Gene Ontology(GO) and Clusters of Orthologous Groups (COG) terms, Enzyme Commission (EC)numbers, and Pfam database domains. A total of 2,305 coding sequences (CDSs) and 66structural RNAs (61 tRNAs) were predicted; 582 CDSs (25%) were classiﬁed as hypo-thetical or uncharacterized proteins. The Rapid Annotations using Subsystems Tech-nology (RAST) server (10) was used for subsystem descriptions. According to RAST, theannotation assigned 923 CDSs (40%) to RAST subsystems.In common with L. helveticus DPC 4571, the genome presents a large number ofinsertion sequences (ISs) (11). It includes 201 mobile element proteins with highsimilarity to transposase enzyme genes, indicating that they may belong to IS elements,which have been shown to speciﬁcally contribute to niche adaptation by promoting avariety of genetic rearrangements. In comparison with CNRZ 32, the genome presentsfour deleted regions ranging from 10 to 40 kb in length. Major gene loss that occurredamong members of the order Lactobacillales indicates early adaptation to nutritionallyrich environments (12). One deletion of interest within contig 11, which mapped withinthe 448212 to 476326 region of CNRZ 32, shows the lack of a cell wall rhamnoseReceived 4 January 2018 Accepted 12January 2018 Published 15 February 2018Citation Palomino MM, Burguener GF, CamposJ, Allievi M, Fina-Martin J, Prado Acosta M,Fernández Do Porto DA, Ruzal SM. 2018. Draftgenome sequence of Lactobacillus helveticusATCC 12046. Genome Announc 6:e01595-17.https://doi.org/10.1128/genomeA.01595-17.Copyright © 2018 Palomino et al. This is anopen-access article distributed under the termsof the Creative Commons Attribution 4.0International license.Address correspondence to Darío A. FernándezDo Porto, dariofd@gmail.com, or Sandra M.Ruzal, sandra@qb.fcen.uba.ar.D.A.F.D.P. and S.M.R. contributed equally to thiswork.PROKARYOTEScrossmVolume 6 Issue 7 e01595-17 genomea.asm.org 1 on February 27, 2018 by guesthttp://genomea.asm.org/Downloaded from containing a polysaccharide cluster. Additionally, in the same deletion we noticed thelack of a glicosyltransferase and an alpha-D-GlcNac-alpha-1,2-L-rhamnosyltransferase,both possibly involved in the side chain formation of rhamnose-glucose cell wallpolysaccharides. The lack of these genes would probably produce variability in thecell wall polysaccharide architecture. It was found that changes in autolytic propertiesobserved in different strains of L. helveticus were due to differences in the chemicalstructure of their cell wall polysaccharides (13). Autolysis of the starter’s strains, as thosebelonging to the L. helveticus species, remains of considerable interest regarding theiruse in dairy fermentation, particularly in the manufacture of cheeses.Accession number(s). This whole-genome shotgun project has been deposited atDDBJ/EMBL/GenBank under the accession number PJRL00000000. The version de-scribed in this paper is version PJRL01000000.ACKNOWLEDGMENTSThis work was supported by grants from the Universidad de Buenos Aires(20020130100444BA and PIP 11220130100162CO) from CONICET (Consejo Nacional deInvestigaciones Cientíﬁcas y Técnicas) to S.M.R. and by grants from the AgenciaNacional de Promoción Cientíﬁca y Tecnológica (ANPCyT PICT-2015-1863) to D.A.F.D.P.M.M.P., M.A., M.P.A., D.A.F.D.P., and S.M.R. are members of CONICET.REFERENCES1. Taverniti V, Guglielmetti S. 2012. Health-promoting properties of Lacto-bacillus helveticus. Front Microbiol 3:392. https://doi.org/10.3389/fmicb.2012.00392.2. Johnson-Henry KC, Hagen KE, Gordonpour M, Tompkins TA, ShermanPM. 2007. Surface-layer protein extracts from Lactobacillus helveticusinhibit enterohaemorrhagic Escherichia coli O157:H7 adhesion to epithe-lial cells. Cell Microbiol 9:356–367. https://doi.org/10.1111/j.1462-5822.2006.00791.x.3. Taverniti V, Stuknyte M, Minuzzo M, Arioli S, De Noni I, Scabiosi C,Cordova ZM, Junttila I, Hämäläinen S, Turpeinen H, Mora D, Karp M, PesuM, Guglielmetti S. 2013. S-layer protein mediates the stimulatory effectof Lactobacillus helveticus MIMLh5 on innate immunity. Appl EnvironMicrobiol 79:1221–1231. https://doi.org/10.1128/AEM.03056-12.4. Meng J, Zhu X, Gao SM, Zhang QX, Sun Z, Lu RR. 2014. Characterizationof surface layer proteins and its role in probiotic properties of threeLactobacillus strains. Int J Biol Macromol 65:110–114. https://doi.org/10.1016/j.ijbiomac.2014.01.024.5. Skrzypczak K, Gustaw W, Was´ko A. 2015. Health-promoting propertiesexhibited by Lactobacillus helveticus strains. Acta Biochim Pol 62:713–720. https://doi.org/10.18388/abp.2015_1116.6. Prado Acosta M, Ruzal SM, Cordo SM. 2016. S-layer proteins fromLactobacillus sp. inhibit bacterial infection by blockage of DC-SIGNcell receptor. Int J Biol Macromol 92:998–1005. https://doi.org/10.1016/j.ijbiomac.2016.07.096.7. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS,Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV,Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA. 2012. SPAdes: a newgenome assembly algorithm and its applications to single-cell sequenc-ing. J Comput Biol 19:455–477. https://doi.org/10.1089/cmb.2012.0021.8. Boetzer M, Henkel CV, Jansen HJ, Butler D, Pirovano W. 2011. Scaffoldingpre-assembled contigs using SSPACE. Bioinformatics 27:578–579. https://doi.org/10.1093/bioinformatics/btq683.9. Sosa EJ, Burguener G, Lanzarotti E, Defelipe L, Radusky L, Pardo AM,Marti M, Turjanski AG, Do Porto DF. 2018. Target-Pathogen: a structuralbioinformatic approach to prioritize drug targets in pathogens. NucleicAcids Res 46:D413–D418. https://doi.org/10.1093/nar/gkx1015.10. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K,Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL,Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD,Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O. 2008.The RAST server: Rapid Annotations using Subsystems Technology. BMCGenomics 9:75. https://doi.org/10.1186/1471-2164-9-75.11. Callanan M, Kaleta P, O’Callaghan J, O’Sullivan O, Jordan K, McAuliffe O,Sangrador-Vegas A, Slattery L, Fitzgerald GF, Beresford T, Ross RP. 2008.Genome sequence of Lactobacillus helveticus, an organism distinguishedby selective gene loss and insertion sequence element expansion. JBacteriol 190:727–735. https://doi.org/10.1128/JB.01295-07.12. Makarova KS, Slesarev A, Wolf Y, Sorokin A, Mirkin B, Koonin EV, PavlovA, Pavlova N, Karamychev V, Polouchine N, Shakhova V, Grigoriev I, LouY, Rohksar D, Lucas S, Huang K, Goodstein DM, Hawkins T, PlengvidhyaV, Welker DL, Hughes JE, Goh Y, Benson A, Baldwin KA, Lee JH, Díaz-Muñiz I, Dosti B, Smeianov VV, Wechter W, Barabote R, Lorca GL,Altermann E, Barrangou R, Ganesan B, Xie Y, Rawsthorne H, Tamir D,Parker C, Breidt F, Broadbent JR, Hutkins R, O’Sullivan D, Steele JL, UnluG, Saier MH, Klaenhammer TR, Richardson P, Kozyavkin S, Weimer BC,Mills DA. 2006. Comparative genomics of the lactic acid bacteria. ProcNatl Acad Sci U S A 103:15611–15616. https://doi.org/10.1073/pnas.0607117103.13. Vinogradov E, Valence F, Maes E, Jebava I, Chuat V, Lortal S, Grard T,Guerardel Y, Sadovskaya I. 2013. Structural studies of the cell wallpolysaccharides from three strains of Lactobacillus helveticus with differ-ent autolytic properties: DPC4571, BROI, and LH1. Carbohydr Res 379:7–12. https://doi.org/10.1016/j.carres.2013.05.020.Palomino et al.Volume 6 Issue 7 e01595-17 genomea.asm.org 2 on February 27, 2018 by guesthttp://genomea.asm.org/Downloaded from 

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Draft genome sequence of Lactobacillus helveticus ATCC 12046

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