Pigmented purpuric dermatosis: a review of the literature

The pigmented purpuric dermatoses (PPDs) are a group of benign, chronic diseases. The variants described to date represent different clinical presentations of the same entity, all having similar histopathologic characteristics. We provide an overview of the most common PPDs and describe their clinical, dermatopathologic, and epiluminescence features. PPDs are both rare and benign, and this, together with an as yet poor understanding of the pathogenic mechanisms involved, means that no standardized treatments exist. We review the treatments described to date. However, because most of the descriptions are based on isolated cases or small series, there is insufficient evidence to support the use of any of these treatments as first-line therapy

Vasculitis leucocitoclástica secundaria a nuevos anticoagulantes orales

Sr. Director: Los nuevos anticoagulantes orales (NACO) son cada vez más empleados en la prevención de ictus en pacientes con fibrilación auricular, y en la prevención y tratamiento de las trombosis venosas profundas y embolismos pulmonares1. Su menor tasa de efectos secundarios y de interacciones farmacológicas, así como su mayor margen terapéutico que permite no realizar controles de laboratorio rutinarios, simplifica su uso en la práctica clínica2. Sin embargo, no están exentos de efectos adversos. Desde el año 2014 han sido descritos 9 casos de vasculitis leucocitoclástica (VL) asociada al consumo de NACO. Aportamos 2 nuevos casos clínicos, así como la primera revisión de la literatura existente hasta la fecha. Presentamos a 2 mujeres de 87 y 84 años, respectivamente, que iniciaron terapia anticoagulante tras detectarse una fibrilación auricular no valvular. La primera consultó 5 meses después del inicio de apixabán por la aparición de lesiones purpúrico-necróticas dolorosas en las 4 extremidades (fig. 1) sin otra clínica sistémica asociada. ..

RNA-binding properties and membrane insertion of Melon necrotic spot virus (MNSV) double gene block movement proteins

Advances in structural and biochemical properties of carmovirus movement proteins (MPs) have only been obtained in p7 and p9 from Carnation mottle virus (CarMV). Alignment of carmovirus MPs revealed a low conservation of amino acid identity but interestingly, similarity was elevated in regions associated with the functional secondary structure elements reported for CarMV which were conserved in all studied proteins. Nevertheless, some differential features in relation with CarMV MPs were identified in those from Melon necrotic virus (MNSV) (p7A and p7B). p7A was a soluble non-sequence specific RNA-binding protein, but unlike CarMV p7, its central region alone could not account for the RNA-binding properties of the entire protein. In fact, a 22-amino acid synthetic peptide whose sequence corresponds to this central region rendered an apparent dissociation constant (K(d)) significantly higher than that of the corresponding entire protein (9 mM vs. 0.83-25.7 microM). This p7A-derived peptide could be induced to fold into an alpha-helical structure as demonstrated for other carmovirus p7-like proteins. Additionally, in vitro fractionation of p7B transcription/translation mixtures in the presence of ER-derived microsomal membranes strongly suggested that p7B is an integral membrane protein. Both characteristics of these two small MPs forming the double gene block (DGB) of MNSV are discussed in the context of the intra- and intercellular movement of carmovirus

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Removal of nitrate from groundwater by nano-scale zero-valent iron injection pulses in continuous-flow packed soil columns

Injection of zero-valent iron nanoparticles (nZVI) into aquifers has gained increasing attention of researchers for in-situ treatment of NO3--contaminated groundwater. nZVI has proved efficient in chemically reducing NO3- and, according to recent research efforts, in supporting biological denitrification under favoured conditions. Given the scarce research on nZVI pulsed injection in continuous-flow systems, the objective of this study was to evaluate the effect of nZVI pulses on the removal of NO3- from groundwater in packed soil columns and, more particularly, to elucidate whether or not biotic NO3- removal processes were promoted by nZVI. Three identical columns were filled with aquifer soil samples and fed with the same nitrate polluted groundwater but operated under different conditions: (A) with application of nZVI pulses and biocide spiked in groundwater, (B) without application of nZVI pulses and (C) with application of nZVI pulses. Results showed that the application of nZVI (at 30 mg/L and 78 mg/L doses) resulted in an immediate and sharp removal of NO3- (88–94%), accompanied by an increase in pH (from 7.0 to 9.0–10.0), a drop in redox potential (Eh) (from +420 mV to 98%) and the NO3- retention capacity of the nZVI particles (13.2–85.5 mg NO3-/g nZVI).Peer ReviewedPostprint (author's final draft