Sialolitiasis parotídea del conducto de Stensen

La litiasis salival es una afectación consistente en la obstrucción mecánica de una glándula salival o de su conducto excretor, debido a la formación de concreciones calcáreas o sialolitos, lo que determina una ectasía salival, pudiendo provocar la dilatación posterior de la glándula. La sialolitiasis supone el 30 % de la patología salival y afecta principalmente a las glándulas submaxilares (83 a 94 %), seguida por la glándula parótida (4 a 10 %) y las glándulas sublinguales (1 a 7 %). En este trabajo presentamos el caso de un paciente varón de 45 años que presentaba mal olor y sabor de boca en el momento de las comidas y afecto de un cálculo salival a nivel del conducto de Stensen izquierdo. Tras el diagnóstico de la sintomatología, el sialolito se eliminó quirúrgicamente bajo anestesia local. De igual forma realizamos una actualización de conceptos en relación con la etiología, diagnóstico y tratamiento de esta patología.Salivary duct lithiasis is a condition characterized by the obstruction of a salivary gland or its excretory duct due to the formation of calcareous concretions or sialoliths resulting in salivary ectasia and even provoking the subsequent dilation of the salivary gland. Sialolithiasis accounts for 30% of salivary diseases and most commonly involves the submaxillary gland (83 to 94%) and less frequently the parotid (4 to 10%) and sublingual glands (1 to 7%). The present study reports the case of a 45-year-old male patient complaining of bad breath and foul-tasting mouth at meal times and presenting with a salivary calculus in left Stensen´s duct. Once the patient was diagnosed, the sialolith was surgically removed using local anesthesia. In this paper we have also updated a series of concepts related to the etiology, diagnosis and treatment of sialolithiasis

Parotid sialolithiasis in Stensen´s duct

Salivary duct lithiasis is a condition characterized by the obstruction of a salivary gland or its excretory duct due to the formation of calcareous concretions or sialoliths resulting in salivary ectasia and even provoking the subsequent dilation of the salivary gland. Sialolithiasis accounts for 30% of salivary diseases and most commonly involves the submaxillary gland (83 to 94%) and less frequently the parotid (4 to 10%) and sublingual glands (1 to 7%). The present study reports the case of a 45-year-old male patient complaining of bad breath and foul-tasting mouth at meal times and presenting with a salivary calculus in left Stensen´s duct. Once the patient was diagnosed, the sialolith was surgically removed using local anesthesia. In this paper we have also updated a series of concepts related to the etiology, diagnosis and treatment of sialolithiasis

Booster effect after SARS-CoV-2 vaccination in immunocompromised hematology patients with prior COVID-19

Patients with hematological malignancies have been excluded from the new zoonotic coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) vaccine trials despite being at higher risk for SARS-CoV-2 disease (COVID-19)-related mortality. However, most health authorities worldwide have designated these patients as a priority for COVID-19 vaccination, even in the absence of efficacy data in these highly immunosuppressed patients. In addition, on 12 August 2021, the US Food and Drug Administration amended the emergency use authorizations for the Pfizer-BioNTech and Moderna COVID-19 vaccines to allow for the use of an additional dose in immunocompromised individuals, such as solid organ transplant recipients or equivalently immunosuppressed patients

A solvatofluorochromic silicon-substituted xanthene dye useful in bioimaging

In this work, we have performed an in-depth study of the photophysics and solvatofluorochromism of a red-emitting Si-xanthene dye, an analog of Tokyo Magenta (TM) historically developed by Egawa et al. (Chem. Commun. 2011, 47, 4162–4164). The results show a strong dependency of the emission properties of 2-Me-4-OMe-TM on the polarity of the solvent. For instance, the dye exhibited an increase in its fluorescence lifetime with the decrease in solvent polarity. Therefore, in this work, this spectral behavior has been used as a new approach for determining the intracellular microenvironment polarity through the measurement of its fluorescence lifetime by Fluorescence Lifetime Imaging Microscopy (FLIM). Our experiments confirmed the ability of the dye to detect changes in polarity between different intracellular compartments.This work was funded by grants CTQ2017-85658-R, CTQ2014-55474-C2-2-R (Spanish Ministry of Economy and Competitiveness; Agencia Estatal de Investigacion, AEI; and European Regional Development Fund, ERDF), QM2012-790 (Junta de Andalucía), and a grant from the Fundación Botín

Evaluation of Burnout Syndrome and Resilience in Jaén Physical Therapists during the COVID-19 Pandemic. Pilot Study

Artículos originales[ES] Introducción: Aunque el síndrome del quemado (burnout) se ha estudiado profusamente en los trabajadores sanitarios,existen pocos estudios que lo aborden específicamente en los fisioterapeutas y cuando esto se ha hecho se ha encontrado que los índices varían desde un nivel bajo hasta un nivel medio-alto. La reciente pandemia COVID-19 ha supuesto y supone un reto notable para el sistema sanitario. En este escenario, puede ser de interés evaluar el índice de burnout en el colectivo de fisioterapeutas.Objetivo: El objetivo de este estudio es medir la prevalencia de burnout en el colectivo de fisioterapeutas de Jaén y provincia, así como estudiar posibles fenómenos asociados a la misma. Material y Métodos: Se remitió una encuesta con datos sociodemográficos, una escala de medición de la resiliencia y una escala de medición del burnout (incluyendo sus tres aspectos: cansancio emocional, despersonalización y realización personal) por medio grupos de WhatsApp de ámbito profesional a los fisioterapeutas de la provincia de Jaén. La encuesta fue anónima, voluntaria y autocumplimentable. Resultados: La prevalencia de burnout entre los fisioterapeutas de la provincia de Jaén es superponible a la publicada en otros estudios. Los fisioterapeutas que no trabajan en la capital sufren más de cansancio emocional (p=0,038) y existe una tendencia a que los hombres sufran más despersonalización. Entre las subescalas de resiliencia que más se asocian al burnout está la de “adaptabilidad y redes de apoyo”.Conclusiones: La frecuencia de burnout en los fisioterapeutas de Jaén es superponible a la publicada por otros autores, no siendo posible establecer si el contexto de pandemia COVID juega algún papel. No trabajar en la capital de la provincia parece favorecer el burnout. El perfil de burnout se concreta sobre todo en los aspectos “cansancio emocional” y “despersonalización”, siendo esto especialmente frecuente en los que presentan un bajo nivel de resiliencia en la subescala “adaptabilidad y redes de apoyo” [EN] Introduction: Although burnout syndrome has been extensively studied in health care workers, there are few studies that specifically address it in physical therapists, and when this has been done, the rates have been found to range from low to medium-high. The recent COVID-19 pandemic has been and continues to be a significant challenge for the healthcare system. In this scenario, it may be of interest to evaluate the burnout index in the group of physical therapists. Objective: The objective of this study is to measure the prevalence of burnout in the group of physical therapists in Jaén and province, as well as to study possible phenomena associated with it. Material and Methods: A survey with sociodemographic data, a resilience measurement scale and a burnout measurement scale (including its three aspects: emotional exhaustion, depersonalization and personal fulfillment) was sent by means of WhatsApp groups of professional scope to the physical therapists of the province from Jaén. The survey was anonymous, voluntary, and self-filling.Results: The prevalence of burnout among physiotherapists in the province of Jaén is superimposable to that published in other studies. Physiotherapists who do not work in the capital suffer more from emotional fatigue (p = 0.038) and there is a tendency for men to suffer more depersonalization. Among the resilience subscales that are most asso ciated with burnout is “adaptability and support networks”. Conclusions: The frequency of burnout in Jaén physiotherapists is superimposable to that published by other authors, and it is not possible to establish whether the context of the COVID pandemic plays any role. Not working in the provincial capital seems to favor burnout. The burnout profile is specified above all in the “emotional fatigue” and “depersonalization” aspects, this being especially frequent in those with a low level of resilience in the “adaptability and support networks” subscale.N

A Brucella melitensis H38¿wbkF rough mutant protects against Brucella ovis in rams

Brucella melitensis and Brucella ovis are gram-negative pathogens of sheep that cause severe economic losses and, although B. ovis is non-zoonotic, B. melitensis is the main cause of human brucellosis. B. melitensis carries a smooth (S) lipopolysaccharide (LPS) with an N-formyl-perosamine O-polysaccharide (O-PS) that is absent in the rough LPS of B. ovis. Their control and eradication require vaccination, but B. melitensis Rev 1, the only vaccine available, triggers anti-O-PS antibodies that interfere in the S-brucellae serodiagnosis. Since eradication and serological surveillance of the zoonotic species are priorities, Rev 1 is banned once B. melitensis is eradicated or where it never existed, hampering B. ovis control and eradication. To develop a B. ovis specific vaccine, we investigated three Brucella live vaccine candidates lacking N-formyl-perosamine O-PS: Bov::CAΔwadB (CO2-independent B. ovis with truncated LPS core oligosaccharide); Rev1::wbdRΔwbkC (carrying N-acetylated O-PS); and H38ΔwbkF (B. melitensis rough mutant with intact LPS core). After confirming their attenuation and protection against B. ovis in mice, were tested in rams for efficacy. H38ΔwbkF yielded similar protection to Rev 1 against B. ovis but Bov::CAΔwadB and Rev1::wbdRΔwbkC conferred no or poor protection, respectively. All H38ΔwbkF vaccinated rams developed a protracted antibody response in ELISA and immunoprecipitation B. ovis diagnostic tests. In contrast, all remained negative in Rose Bengal and complement fixation tests used routinely for B. melitensis diagnosis, though some became positive in S-LPS ELISA owing to LPS core epitope reactivity. Thus, H38ΔwbkF is an interesting candidate for the immunoprophylaxis of B. ovis in B. melitensis-free areas

Novel deep targeted sequencing method for minimal residual disease monitoring in acute myeloid leukemia

A high proportion of patients with acute myeloid leukemia who achieve minimal residual disease (MRD) negative status ultimately relapse because a fraction of pathological clones remains undetected by standard methods. We designed and validated a high-throughput sequencing method for MRD assessment of cell clonotypes with mutations of NPM1, IDH1/2 and/or FLT3-SNVs. For clinical validation, 106 follow-up samples from 63 patients in complete remission were studied by NGS, evaluating the level of mutations detected at diagnosis. The predictive value of MRD status by NGS, multiparameter flow cytometry, or quantitative PCR was determined by survival analysis. The method achieved a sensitivity of 10-4 for SNV mutations and 10-5 for insertions/deletions and could be used in acute myeloid leukemia patients who carry any mutation (86% in our diagnosis data set). NGS-determined MRD positive status was associated with lower disease-free survival (hazard ratio [HR] 3.4, p=0.005) and lower overall survival (HR 4.2, p<0.001). Multivariate analysis showed that MRD positive status by NGS was an independent factor associated with risk of death (HR 4.54, p =0.005) and the only independent factor conferring risk of relapse (HR 3.76, p =0.012). This NGS based method simplifies and standardizes MRD evaluation, with high applicability in acute myeloid leukemia. It also improves upon flow cytometry and quantitative PCR to predict acute myeloid leukemia outcome and could be incorporated in clinical settings and clinical trials.This study was supported by the Subdirección General de Investigación Sanitaria (Instituto de Salud Carlos III, Spain) grants PI13/02387 and PI16/01530, and the CRIS against Cancer foundation grant 2014/0120. M.L. holds a postdoctoral fellowship of the Spanish Ministry of Economy and Competitiveness (FPDI-2013-16409). P.R.P. holds a postdoctoral fellowship of the Spanish of Instituto de Salud Carlos III: Contrato Predoctoral de Formación en Investigación en Salud i-PFIS (IFI 14/00008).S

Scientific collaboration for early detection of invaders results in a significant update on estimated range: lessons from Stenothoe georgiana Bynum & Fox 1977

Detection of new non-indigenous species is often delayed when taxonomically challenging taxa are involved, such as small-sized marine organisms. The present study highlights the relevance of scientific cooperation in the early detection of the invader amphipod Stenothoe georgiana. Originally described from North Carolina (USA), the species was recently found in Chile and the Western Mediterranean. Here, we provide the first record of the species in Macaronesia, Atlantic coasts of continental Europe, North Africa and Australia, and extend its known distribution along the Mediterranean coast. Just like other small crustaceans, shipping (including recreational boating) and aquaculture are probably the main vectors of introduction and secondary spread for this amphipod species. This case of S. georgiana sheds light on the importance of promoting taxonomical knowledge, and building multidisciplinary expertise networks that ensure an effective spread of alien species information. We also encourage the implementation of standardized monitoring methodologies to facilitate early detection of small mobile invaders

A Brucella melitensis H38ΔwbkF rough mutant protects against Brucella ovis in rams

Brucella melitensis and Brucella ovis are gram-negative pathogens of sheep that cause severe economic losses and, although B. ovis is non-zoonotic, B. melitensis is the main cause of human brucellosis. B. melitensis carries a smooth (S) lipopolysaccharide (LPS) with an N-formyl-perosamine O-polysaccharide (O-PS) that is absent in the rough LPS of B. ovis. Their control and eradication require vaccination, but B. melitensis Rev 1, the only vaccine available, triggers anti-O-PS antibodies that interfere in the S-brucellae serodiagnosis. Since eradication and serological surveillance of the zoonotic species are priorities, Rev 1 is banned once B. melitensis is eradicated or where it never existed, hampering B. ovis control and eradication. To develop a B. ovis specific vaccine, we investigated three Brucella live vaccine candidates lacking N-formyl-perosamine O-PS: Bov::CAΔwadB (CO2-independent B. ovis with truncated LPS core oligosaccharide); Rev1::wbdRΔwbkC (carrying N-acetylated O-PS); and H38ΔwbkF (B. melitensis rough mutant with intact LPS core). After confirming their attenuation and protection against B. ovis in mice, were tested in rams for efficacy. H38ΔwbkF yielded similar protection to Rev 1 against B. ovis but Bov::CAΔwadB and Rev1::wbdRΔwbkC conferred no or poor protection, respectively. All H38ΔwbkF vaccinated rams developed a protracted antibody response in ELISA and immunoprecipitation B. ovis diagnostic tests. In contrast, all remained negative in Rose Bengal and complement fixation tests used routinely for B. melitensis diagnosis, though some became positive in S-LPS ELISA owing to LPS core epitope reactivity. Thus, H38ΔwbkF is an interesting candidate for the immunoprophylaxis of B. ovis in B. melitensis-free areas.Publishe