Shoreline Changes and Coastal Erosion: The Case Study of the Coast of Togo (Bight of Benin, West Africa Margin)

The coastal strip between the Volta River delta and the westernmost portion of Benin (West Africa Margin of Atlantic Basin) is highly populated (e.g., Lomé) due to migrations from inland areas. The coastal zone has proved to be very vulnerable because of the potential development of sometimes catastrophic events related to different and interacting causes, resulting in negative effects on natural ecosystems and socio-economic conditions. The main problem is the marked erosion of large coastal sectors with maximum retreat rates of the order of 5 m/year. The continuous loss of territory leads to a progressive impoverishment of activities and human resources and to the increase of geological risk factors. The coastal erosion is induced both by natural and anthropic causes and can be controlled only by means of prevention programs, detailed scientific studies and targeted technical interventions. The main erosional processes observed in the study area are triggered by the presence of the Lomé port and other human activities on the coastal strip, including the water extraction from the subsoil, which induces subsidence and the use of sediments as inert material. These elements, together with the reduction of the solid supply from Volta River (caused by the realization of the Akosombo dam) are among the main factors that control the medium and long-term evolution of the area. Also relative sea level changes, which take into account also tectonic and/or isostatic components, can contribute to the process. In order to have a real understanding of the coastal dynamics and evolution, it would be necessary to develop a scientific structure through the collaboration of all countries of the Bight of Benin affected by coastal erosion. The aim should be primarily to collect the interdisciplinary quantitative data necessary to develop a scientific knowledge background of the Bight of Benin coastal/ocean system. In conclusion, some proposals are presented to reduce the vulnerability of the coastal area as for example to plan surveys for the realization of appropriate coastal protection works, such as walls, revetments, groins, etc. A possible expansion of the port of Lomè is also considered. Proposals comprise the constitution of a Supranational Scientific Committee as a coordinating structure on erosion for both the study of phenomena and planning interventions.Research supported by University of Urbino Carlo Bo (funds Ricerca Scientifica 2020, DiSPeA-M. Tramontana); Research Project CGL2016-75679-P, Spanish Ministry of Education and Science; Research Groups, Projects of the Generalitat Valenciana, Alicante University (CTMA-IGA)

Decellularized diaphragmatic muscle drives a constructive angiogenic response in vivo

Skeletal muscle tissue engineering (TE) aims to efficiently repair large congenital and acquired defects. Biological acellular scaffolds are considered a good tool for TE, as decellularization allows structural preservation of tissue extracellular matrix (ECM) and conservation of its unique cytokine reservoir and the ability to support angiogenesis, cell viability, and proliferation. This represents a major advantage compared to synthetic scaffolds, which can acquire these features only after modification and show limited biocompatibility. In this work, we describe the ability of a skeletal muscle acellular scaffold to promote vascularization both ex vivo and in vivo. Specifically, chicken chorioallantoic membrane assay and protein array confirmed the presence of pro-angiogenic molecules in the decellularized tissue such as HGF, VEGF, and SDF-1\u3b1. The acellular muscle was implanted in BL6/J mice both subcutaneously and ortotopically. In the first condition, the ECM-derived scaffold appeared vascularized 7 days post-implantation. When the decellularized diaphragm was ortotopically applied, newly formed blood vessels containing CD31+, \u3b1SMA+, and vWF+ cells were visible inside the scaffold. Systemic injection of Evans Blue proved function and perfusion of the new vessels, underlying a tissue-regenerative activation. On the contrary, the implantation of a synthetic matrix made of polytetrafluoroethylene used as control was only surrounded by vWF+ cells, with no cell migration inside the scaffold and clear foreign body reaction (giant cells were visible). The molecular profile and the analysis of macrophages confirmed the tendency of the synthetic scaffold to enhance inflammation instead of regeneration. In conclusion, we identified the angiogenic potential of a skeletal muscle-derived acellular scaffold and the pro-regenerative environment activated in vivo, showing clear evidence that the decellularized diaphragm is a suitable candidate for skeletal muscle tissue engineering and regeneration

Cluster-Cluster Lensing and the Case of Abell 383

Extensive surveys of galaxy clusters motivate us to assess the likelihood of cluster-cluster lensing (CCL), namely, gravitational-lensing of a background cluster by a foreground cluster. We briefly describe the characteristics of CCLs in optical, X-ray and SZ measurements, and calculate their predicted numbers for $\Lambda$CDM parameters and a viable range of cluster mass functions and their uncertainties. The predicted number of CCLs in the strong-lensing regime varies from several ($<10$) to as high as a few dozen, depending mainly on whether lensing triaxiality bias is accounted for, through the c-M relation. A much larger number is predicted when taking into account also CCL in the weak-lensing regime. In addition to few previously suggested CCLs, we report a detection of a possible CCL in A383, where background candidate high-$z$ structures are magnified, as seen in deep Subaru observations.Comment: 9 pages, 5 figures, submitted to MNRA

Molecular characterization of Mycobacterium tuberculosis and Mycobacterium bovis isolates by Enterobacterial Repetitive Intergenic Consensus-PCR

Tuberculosis (TB) is an infectious disease in which the molecular typing methods allow to have important information about the dynamics of transmission and to assist properly in disease control. Although the ERIC-PCR (Enterobacterial repetitive intergenic consensus-PCR) assay is fast and easy to perform, scarce studies have reported its use in epidemiological studies in TB outbreaks. In this study, we aimed to genotype Mycobacterium tuberculosis and M. bovis isolates by ERIC-PCR and compare its discriminatory power with two other classically used methods: 12 loci-MIRU (Mycobacterial Interspersed Repetitive Units) and Spoligotyping. The M. tuberculosis isolates studied were from northwestern and southwestern and M. bovis from northwestern Parana, Brazil. ERIC-PCR rendered banding patterns with great diversity (1 to 12 bands) of molecular sizes, ranging from 100 to 1600 bp. ERIC-PCR showed to be fast, simple and affordable to differentiate isolates. ERIC-PCR would be an important tool in the epidemiology of TB as screening in case of outbreak, which demands rapid intervention. However if any doubt persist, as it may occur with the application of only one genotypic method, other genotyping methods should be applied and carefully interpreted, always with additional epidemiological information

Charge Properties of TiO2 Nanotubes in NaNO3 Aqueous Solution

Charging of material surfaces in aqueous electrolyte solutions is one of the most important processes in the interactions between biomaterials and surrounding tissue. Other than a biomaterial, titania nanotubes (TiO2 NTs) represent a versatile material for numerous applications such as heavy metal adsorption or photocatalysis. In this article, the surface charge properties of titania NTs in NaNO3 solution were investigated through electrophoretic mobility and polyelectrolyte colloid titration measuring techniques. In addition, we used high-resolution transmission electron microscopy imaging to determine the morphology of TiO2 NTs. A theoretical model based on the classical density functional theory coupled with the charge regulation method in terms of mass action law was developed to understand the experimental data and to provide insights into charge properties at different physical conditions, namely, pH and NaNO3 concentration. Two intrinsic protonation constants and surface site density have been obtained. The electrostatic properties of the system in terms of electrostatic potentials and ion distributions were calculated and discussed for various pH values. The model can quantitatively describe the titration curve as a function of pH for higher bulk salt concentrations and the difference in the equilibrium amount of charges between the inner and outer surfaces of TiO2 NTs. Calculated counterion (NO3–) distributions show a pronounced decrease of NO3– ions for high bulk pH (both inside and outside TiO2 NT) because of the strong electric field. With the decrease of bulk pH or the increase of the salt concentration, NO3– is able to accumulate near the TiO2 NTs surfaces

Generalised massive gravity one-loop partition function and AdS/(L)CFT

The graviton 1-loop partition function is calculated for Euclidean generalised massive gravity (GMG) using AdS heat kernel techniques. We find that the results fit perfectly into the AdS/(L)CFT picture. Conformal Chern-Simons gravity, a singular limit of GMG, leads to an additional contribution in the 1-loop determinant from the conformal ghost. We show that this contribution has a nice interpretation on the conformal field theory side in terms of a semi-classical null vector at level two descending from a primary with conformal weights (3/2,-1/2).Comment: 25 p., 2 jpg figs, v2: added 6 lines of clarifying text after Eq. (2.38

Neutralino-Nucleon Cross Section and Charge and Colour Breaking Constraints

We compute the neutralino-nucleon cross section in several supersymmetric scenarios, taking into account all kind of constraints. In particular, the constraints that the absence of dangerous charge and colour breaking minima imposes on the parameter space are studied in detail. In addition, the most recent experimental constraints, such as the lower bound on the Higgs mass, the $b\to s\gamma$ branching ratio, and the muon $g-2$ are considered. The astrophysical bounds on the dark matter density are also imposed on the theoretical computation of the relic neutralino density, assuming thermal production. This computation is relevant for the theoretical analysis of the direct detection of dark matter in current experiments. We consider first the supergravity scenario with universal soft terms and GUT scale. In this scenario the charge and colour breaking constraints turn out to be quite important, and \tan\beta\lsim 20 is forbidden. Larger values of $\tan\beta$ can also be forbidden, depending on the value of the trilinear parameter $A$. Finally, we study supergravity scenarios with an intermediate scale, and also with non-universal scalar and gaugino masses where the cross section can be very large.Comment: Final version to appear in JHE

Regulation of DNA Methylation Patterns by CK2-Mediated Phosphorylation of Dnmt3a

DNA methylation is a central epigenetic modification that is established by de novo DNA methyltransferases. The mechanisms underlying the generation of genomic methylation patterns are still poorly understood. Using mass spectrometry and a phosphospecific Dnmt3a antibody, we demonstrate that CK2 phosphorylates endogenous Dnmt3a at two key residues located near its PWWP domain, thereby downregulating the ability of Dnmt3a to methylate DNA. Genome-wide DNA methylation analysis shows that CK2 primarily modulates CpG methylation of several repeats, most notably of Alu SINEs. This modulation can be directly attributed to CK2-mediated phosphorylation of Dnmt3a. We also find that CK2-mediated phosphorylation is required for localization of Dnmt3a to heterochromatin. By revealing phosphorylation as a mode of regulation of de novo DNA methyltransferase function and by uncovering a mechanism for the regulation of methylation at repetitive elements, our results shed light on the origin of DNA methylation patterns

The third data release of the Kilo-Degree Survey and associated data products

The Kilo-Degree Survey (KiDS) is an ongoing optical wide-field imaging survey with the OmegaCAM camera at the VLT Survey Telescope. It aims to image 1500 square degrees in four filters (ugri). The core science driver is mapping the large-scale matter distribution in the Universe, using weak lensing shear and photometric redshift measurements. Further science cases include galaxy evolution, Milky Way structure, detection of high-redshift clusters, and finding rare sources such as strong lenses and quasars. Here we present the third public data release (DR3) and several associated data products, adding further area, homogenized photometric calibration, photometric redshifts and weak lensing shear measurements to the first two releases. A dedicated pipeline embedded in the Astro-WISE information system is used for the production of the main release. Modifications with respect to earlier releases are described in detail. Photometric redshifts have been derived using both Bayesian template fitting, and machine-learning techniques. For the weak lensing measurements, optimized procedures based on the THELI data reduction and lensfit shear measurement packages are used. In DR3 stacked ugri images, weight maps, masks, and source lists for 292 new survey tiles (~300 sq.deg) are made available. The multi-band catalogue, including homogenized photometry and photometric redshifts, covers the combined DR1, DR2 and DR3 footprint of 440 survey tiles (447 sq.deg). Limiting magnitudes are typically 24.3, 25.1, 24.9, 23.8 (5 sigma in a 2 arcsec aperture) in ugri, respectively, and the typical r-band PSF size is less than 0.7 arcsec. The photometric homogenization scheme ensures accurate colors and an absolute calibration stable to ~2% for gri and ~3% in u. Separately released are a weak lensing shear catalogue and photometric redshifts based on two different machine-learning techniques.Comment: small modifications; 27 pages, 12 figures, accepted for publication in Astronomy & Astrophysic