Does Portfolio-Based Language Assessment Align with Learning-Oriented Assessment? Evidence from Literacy Learners and their Instructors

A high-stakes Portfolio-Based Language Assessment (PBLA) protocol that was fully implemented in all Language Instruction for Newcomers to Canada (LINC) programs in 2019 requires instructors and students to set language-learning goals and complete, compile, and reflect on numerous authentic language tasks. Due to the language barriers incurred when communicating with beginner English-as-a-second-language literacy learners (BELLs), no PBLA research has been conducted with BELLs. To address this gap, we interviewed 26 BELLs (n = 2 from 13 L1s) and their instructors (n = 4) about their understanding and use of PBLA. Student interviews were conducted with the assistance of bilingual interpreters in the students’ L1s. All the interviews were then transcribed and thematically analyzed in relation to PBLA’s alignment with the six dimensions in Turner and Purpura’s (2016) learning-oriented assessment framework: contextual, elicitation, proficiency, learning, instructional, interactional, and affective. Results have implications for optimizing learning, and task-based instruction and assessment practices in LINC.Le protocole d’évaluation linguistique basé sur le portefolio (Portfolio-Based Language Assessment - PBLA) à enjeux élevés a été entièrement mis en œuvre dans tous les programmes des cours de langue pour les immigrants au Canada (Language instruction for Newcomers to Canada - LINC) en 2019.  Ce protocole exige que les enseignants et les élèves fixent des objectifs d'apprentissage des langues et accomplissent, compilent et réfléchissent à de nombreuses tâches linguistiques authentiques. En raison des barrières linguistiques rencontrées lors de la communication avec des apprenants débutants en anglais langue seconde peu littératiés, aucune recherche PBLA n'a été menée auprès des apprenants. Pour combler cette lacune, nous avons interviewé 26 apprenants (n = 2 de 13 L1) et leurs enseignants (n = 4) au sujet de leur compréhension et de leur utilisation du PBLA. Les entretiens avec les élèves ont été menés avec l’aide d’interprètes bilingues dans les L1 des élèves. Ensuite, tous les entretiens ont été transcrits et analysés de manière thématique en relation avec l'alignement du PBLA avec les six dimensions du cadre d'évaluation axé sur l'apprentissage de Turner et Purpura (2016): contextuel, élicitation, compétence, apprentissage, pédagogique, interactionnel et affectif. Les résultats ont des implications pour l'optimisation de l'apprentissage et les pratiques d'enseignement et d'évaluation basées sur les tâches dans l'enseignement de la langue pour les nouveaux arrivants au Canada

A Focus Group Study Of Agrlcultural Marketing Programs

Five focus group sessions were held involving over 50 agricultural producers and professionals who work with farmers

Enhancing the Impact of Evidence-based Publications on K-12 ESL Teacher Practices

The reading of current research-informed publications is an essential component of teacher professional development that has the potential to lead to or reinforce the implementation of effective instructional practices. To our knowledge, no studies have examined kindergarten to grade 12 (K-12) ESL teacher engagement in professional reading related to the teaching and learning of ESL. Therefore, we conducted a survey of K-12 ESL teachers from four Canadian provinces to examine their reading practices. Results revealed that teachers mainly consulted professional newsletter articles to address classroom-related issues and indicated that key stakeholders (e.g., teachers’ associations, school districts, and school principals) do little to enhance teachers’ reading of TESL-related publications. Several recommendations to increase teacher reading engagement are included.La lecture de publications courantes fondées sur la recherche constitue une composante essentielle du développement professionnel des enseignants et une qui a le potentiel d’entrainer la mise en œuvre de pratiques pédagogiques efficaces, ou de renforcer celles-ci. Nous ne connaissons aucune étude ayant examiné l’implication des enseignants K-12 dans la lecture de revues et d’ouvrages spécialisés portant sur l’enseignement et l’apprentissage en ALS. Nous avons donc entrepris une enquête auprès d’enseignants K-12 d’ALS dans quatre provinces canadiennes pour connaitre leurs pratiques quant à la lecture. Les résultats indiquent que les enseignants consultaient surtout des articles de bulletins professionnels pour aborder des questions liées à leur pratique. Les enseignants ont également mentionné que les intervenants principaux (par ex. les associations d’enseignants, les districts scolaires et les directeurs d’école) faisaient peu pour encourager la lecture par les enseignants de publications portant sur l’ALS. Nous présentons plusieurs recommandations pour motiver les enseignants à la lecture

Quantifying the Increase in Radiation Exposure Associated with SPECT/CT Compared to SPECT Alone for Routine Nuclear Medicine Examinations

Purpose. We quantify the additional radiation exposure in terms of effective dose incurred by patients in the CT portion of SPECT/CT examinations. Methods. The effective dose from a variety of common nuclear medicine procedures is calculated and summarized. The extra exposure from the CT portion of the examination is summarized by examination and body part. Two hundred forty-eight scans from 221 patients are included in this study. The effective dose from the CT examination is also compared to average background radiation. Results. We found that the extra effective dose is not sufficient to cause deterministic effects. However, the stochastic effects may be significant, especially in patients undergoing numerous follow-up studies. The cumulative effect might increase the radiation exposure compared to patient management with SPECT alone. Conclusions. While the relative increase in radiation exposure associated with SPECT/CT is generally considered acceptable when compared with the benefits to the patient, physicians should make every effort to minimize this effect by using proper technical procedures and educating patients about the exposure they will receive

Do production patterns influence the processing of speech in prelinguistic infants?

The headturn preference procedure was used to test 18 infants on their response to three different passages chosen to reflect their individual production patterns. The passages contained nonwords with consonants in one of three categories: (a) often produced by that infant (‘own’), (b) rarely produced by that infant but common at that age (‘other’), and (c) not generally produced by infants. Infants who had a single ‘own’ consonant showed no significant preference for either ‘own’ (a) or ‘other’ (b) passages. In contrast, infants’ with two ‘own’ consonants exhibited greater attention to ‘other’ passages (b). Both groups attended equally to the passage featuring consonants rarely produced by infants of that age (c). An analysis of a sample of the infant-directed speech ruled out the mothers’ speech as a source of the infant preferences. The production-based shift to a focus on the ‘other’ passage suggests that nascent production abilities combine with emergent perceptual experience to facilitate word learning

The Evolutionary Analysis of Emerging Low Frequency HIV-1 CXCR4 Using Variants through Time—An Ultra-Deep Approach

Large-scale parallel pyrosequencing produces unprecedented quantities of sequence data. However, when generated from viral populations current mapping software is inadequate for dealing with the high levels of variation present, resulting in the potential for biased data loss. In order to apply the 454 Life Sciences' pyrosequencing system to the study of viral populations, we have developed software for the processing of highly variable sequence data. Here we demonstrate our software by analyzing two temporally sampled HIV-1 intra-patient datasets from a clinical study of maraviroc. This drug binds the CCR5 coreceptor, thus preventing HIV-1 infection of the cell. The objective is to determine viral tropism (CCR5 versus CXCR4 usage) and track the evolution of minority CXCR4-using variants that may limit the response to a maraviroc-containing treatment regimen. Five time points (two prior to treatment) were available from each patient. We first quantify the effects of divergence on initial read k-mer mapping and demonstrate the importance of utilizing population-specific template sequences in relation to the analysis of next-generation sequence data. Then, in conjunction with coreceptor prediction algorithms that infer HIV tropism, our software was used to quantify the viral population structure pre- and post-treatment. In both cases, low frequency CXCR4-using variants (2.5–15%) were detected prior to treatment. Following phylogenetic inference, these variants were observed to exist as distinct lineages that were maintained through time. Our analysis, thus confirms the role of pre-existing CXCR4-using virus in the emergence of maraviroc-insensitive HIV. The software will have utility for the study of intra-host viral diversity and evolution of other fast evolving viruses, and is available from http://www.bioinf.manchester.ac.uk/segminator/

Social Motility in African Trypanosomes

African trypanosomes are devastating human and animal pathogens that cause significant human mortality and limit economic development in sub-Saharan Africa. Studies of trypanosome biology generally consider these protozoan parasites as individual cells in suspension cultures or in animal models of infection. Here we report that the procyclic form of the African trypanosome Trypanosoma brucei engages in social behavior when cultivated on semisolid agarose surfaces. This behavior is characterized by trypanosomes assembling into multicellular communities that engage in polarized migrations across the agarose surface and cooperate to divert their movements in response to external signals. These cooperative movements are flagellum-mediated, since they do not occur in trypanin knockdown parasites that lack normal flagellum motility. We term this behavior social motility based on features shared with social motility and other types of surface-induced social behavior in bacteria. Social motility represents a novel and unexpected aspect of trypanosome biology and offers new paradigms for considering host-parasite interactions

Fine-Scale Variation in Vector Host Use and Force of Infection Drive Localized Patterns of West Nile Virus Transmission

The influence of host diversity on multi-host pathogen transmission and persistence can be confounded by the large number of species and biological interactions that can characterize many transmission systems. For vector-borne pathogens, the composition of host communities has been hypothesized to affect transmission; however, the specific characteristics of host communities that affect transmission remain largely unknown. We tested the hypothesis that vector host use and force of infection (i.e., the summed number of infectious mosquitoes resulting from feeding upon each vertebrate host within a community of hosts), and not simply host diversity or richness, determine local infection rates of West Nile virus (WNV) in mosquito vectors. In suburban Chicago, Illinois, USA, we estimated community force of infection for West Nile virus using data on Culex pipiens mosquito host selection and WNV vertebrate reservoir competence for each host species in multiple residential and semi-natural study sites. We found host community force of infection interacted with avian diversity to influence WNV infection in Culex mosquitoes across the study area. Two avian species, the American robin (Turdus migratorius) and the house sparrow (Passer domesticus), produced 95.8% of the infectious Cx. pipiens mosquitoes and showed a significant positive association with WNV infection in Culex spp. mosquitoes. Therefore, indices of community structure, such as species diversity or richness, may not be reliable indicators of transmission risk at fine spatial scales in vector-borne disease systems. Rather, robust assessment of local transmission risk should incorporate heterogeneity in vector host feeding and variation in vertebrate reservoir competence at the spatial scale of vector-host interaction

Genetic diversity fuels gene discovery for tobacco and alcohol use

Tobacco and alcohol use are heritable behaviours associated with 15% and 5.3% of worldwide deaths, respectively, due largely to broad increased risk for disease and injury(1-4). These substances are used across the globe, yet genome-wide association studies have focused largely on individuals of European ancestries(5). Here we leveraged global genetic diversity across 3.4 million individuals from four major clines of global ancestry (approximately 21% non-European) to power the discovery and fine-mapping of genomic loci associated with tobacco and alcohol use, to inform function of these loci via ancestry-aware transcriptome-wide association studies, and to evaluate the genetic architecture and predictive power of polygenic risk within and across populations. We found that increases in sample size and genetic diversity improved locus identification and fine-mapping resolution, and that a large majority of the 3,823 associated variants (from 2,143 loci) showed consistent effect sizes across ancestry dimensions. However, polygenic risk scores developed in one ancestry performed poorly in others, highlighting the continued need to increase sample sizes of diverse ancestries to realize any potential benefit of polygenic prediction.Peer reviewe

Genetic determinants of telomere length from 109,122 ancestrally diverse whole-genome sequences in TOPMed

Genetic studies on telomere length are important for understanding age-related diseases. Prior GWAS for leukocyte TL have been limited to European and Asian populations. Here, we report the first sequencing-based association study for TL across ancestrally-diverse individuals (European, African, Asian and Hispanic/Latino) from the NHLBI Trans-Omics for Precision Medicine (TOPMed) program. We used whole genome sequencing (WGS) of whole blood for variant genotype calling and the bioinformatic estimation of telomere length in n=109,122 individuals. We identified 59 sentinel variants (p-value OBFC1indicated the independent signals colocalized with cell-type specific eQTLs for OBFC1 (STN1). Using a multi-variant gene-based approach, we identified two genes newly implicated in telomere length, DCLRE1B (SNM1B) and PARN. In PheWAS, we demonstrated our TL polygenic trait scores (PTS) were associated with increased risk of cancer-related phenotypes