Prevalencia de Entamoeba gingivalis en pacientes con enfermedad periodontal cronica

44 p.Hace años se ha descrito el posible papel que tendría E. gingivalis en la patogenia de la enfermedad periodontal y de otras afecciones estomatológicas como: caries dentales, periodontopatías, pulpitis, gangrenas, estomatitis ulcero-necrótica, entre otras. En Chile no se han reportado casos o estudios que den cuenta de la prevalencia de este protozoo, por lo tanto, el propósito central de este trabajo es conocer la prevalencia de Entamoeba gingivalis en pacientes con enfermedad periodontal que asistieron al Hospital Regional de Talca, Consultorio Dionisio Astaburuaga y Centro de Salud Familiar Carlos Trupp (CESFAM) entre el periodo marzo-mayo de 2009. Para la identificación de E. gingivalis la metodología utilizada fue, la obtención de muestras de líquido crevicular tomadas desde sacos periodontales activos y de placa y/o cálculo dental a 96 pacientes de ambos sexos. A cada una de las muestras obtenidas se les realizó un examen directo y cultivo. Los resultados obtenidos de este estudio arrojaron que existe una prevalencia de Entamoeba gingivalis de 16,7%; además se determinó que existía asociación entre la presencia de Entamoeba gingivalis y las variables sexo y severidad de la Enfermedad Periodontal

Characterization of digestive involvement in patients with chronic T. cruzi infection in Barcelona, Spain

Background: Digestive damage due to Chagas disease (CD) occurs in 15-20% of patients diagnosed as a result of peristaltic dysfunction in some endemic areas. The symptoms of chronic digestive CD are non-specific, and there are numerous confounders. Diagnosis of CD may easily be missed if symptoms are not evaluated by a well trained physician. Regular tests, as barium contrast examinations, probably lack the necessary sensitivity to detect early digestive damage. Methods: 71 individuals with T. cruzi infection (G1) and 18 without (G2) coming from Latin American countries were analyzed. They were asked for clinical and epidemiological data, changes in dietary habits, and history targeting digestive and cardiac CD symptoms. Serological tests for T. cruzi, barium swallow, barium enema, an urea breath test, and esophageal manometry were requested for all patients. Principal findings: G1 and G2 patients did not show differences in lifestyle and past history. Fifteen (21.1%) of G1 had digestive involvement. Following Rezende criteria, esophagopathy was observed in 8 patients in G1 (11.3%) and in none of those in G2. Manometry disorders were recorded in 34 G1 patients and in six in G2. Isolated hypotensive lower esophageal sphincter (LES) was found in sixteen G1 patients (23.9%) and four G2 patients (28.8%). Achalasia was observed in two G1 patients. Among G1 patients, ineffective esophageal motility was seen in six (five with symptoms), diffuse esophageal spasm in two (one with dysphagia and regurgitation), and nutcracker esophagus in three (all with symptoms). There were six patients with hypertonic upper esophageal sphincter (UES) among G1. Following Ximenes criteria, megacolon was found in ten G1 patients (13.9%), and in none of the G2 patients. Conclusions: The prevalence of digestive chronic CD in our series was 21.1%. Dysphagia is a non-pathognomonic symptom of CD, but a good marker of early esophageal involvement. Manometry could be a useful diagnostic test in selected cases, mainly in patients with T. cruzi infection and dysphagia in whose situation barium swallow does not evidence alterations. Constipation is a common but non-specific symptom that can be easily managed. Testing for CD is mandatory in a patient from Latin America with constipation or dysphagia, and if diagnosis is confirmed, megacolon and esophageal involvement should be investigated

A produção de revista como método de ensino em Botânica: relato de experiência de licenciandas em Ciências Biológicas

O ensino de Botânica ainda é rodeado por diversos desafios, dentre eles está a transmissão dos assuntos apenas de maneira conteudista, o que acaba por alimentar a cegueira botânica. Nesse sentido, é primordial que os professores desenvolvam estratégias que busquem consolidar a aprendizagem dos estudantes, sendo a elaboração de materiais didáticos uma alternativa. Assim, o objetivo dessa pesquisa é descrever a experiência de Licenciandas em Ciências Biológicas no desenvolvimento de uma revista com temas relacionados à Botânica. A revista contou com sessões como reportagem, peça teatral e paródia e se mostrou como um método de ensino eficiente tanto para licenciandos como para seus futuros alunos

Prognostic value of discharge heart rate in acute heart failure patients: More relevant in atrial fibrillation?

Aims: The prognostic impact of heart rate (HR) in acute heart failure (AHF) patients is not well known especially in atrial fibrillation (AF) patients. The aim of the study was to evaluate the impact of admission HR, discharge HR, HR difference (admission-discharge) in AHF patients with sinus rhythm (SR) or AF on long- term outcomes. Methods: We included 1398 patients consecutively admitted with AHF between October 2013 and December 2014 from a national multicentre, prospective registry. Logistic regression models were used to estimate the association between admission HR, discharge HR and HR difference and one- year all-cause mortality and HF readmission. Results: The mean age of the study population was 72+/-12years. Of these, 594 (42.4%) were female, 655 (77.8%) were hypertensive and 655 (46.8%) had diabetes. Among all included patients, 745 (53.2%) had sinus rhythm and 653 (46.7%) had atrial fibrillation. Only discharge HR was associated with one year all-cause mortality (Relative risk (RR)=1.182, confidence interval (CI) 95% 1.024-1.366, p=0.022) in SR. In AF patients discharge HR was associated with one year all cause mortality (RR=1.276, CI 95% 1.115-1.459, p</=0.001). We did not observe a prognostic effect of admission HR or HRD on long-term outcomes in both groups. This relationship is not dependent on left ventricular ejection fraction. Conclusions: In AHF patients lower discharge HR, neither the admission nor the difference, is associated with better long-term outcomes especially in AF patients

Diaphragm Pacing in Patients with Spinal Cord Injury:A European Experience

BACKGROUND: Patients with high spinal cord injury (SCI) are unable to breathe on their own and require mechanical ventilation (MV). The long-term use of MV is associated with increased morbidity and mortality. In patients with intact phrenic nerve function, patients can be partially or completely removed from MV by directly stimulating the diaphragm motor points with a diaphragm pacing system (DPS). OBJECTIVES: We describe our multicenter European experience using DPS in SCI patients who required MV. METHODS: We conducted a retrospective study of patients who were evaluated for the implantation of DPS. Patients evaluated for DPS who met the prospectively defined criteria of being at least 1 year of age, and having cervical injury resulting in a complete or partial dependency on MV were included. Patients who received DPS implants were followed for up to 1 year for device usage and safety. RESULTS: Across 3 centers, 47 patients with high SCI were evaluated for DPS, and 34 were implanted. Twenty-one patients had 12 months of follow-up data with a median DPS use of 15 h/day (interquartile range 4, 24). Eight patients (38.1%) achieved complete MV weaning using DPS 24 h/day. Two DPS-related complications were surgical device revision and a wire eruption. No other major complications were associated with DPS use. CONCLUSIONS: Diaphragm pacing represents an attractive alternative stand-alone treatment or adjunctive therapy compared to MV in patients with high SCI. After a period of acclimation, the patients were able to reduce the daily use of MV, and many could be completely removed from MV

Association Between Western and Mediterranean Dietary Patterns and Mammographic Density

OBJECTIVE: To examine the association between two dietary patterns (Western and Mediterranean), previously linked to breast cancer risk, and mammographic density. METHODS: This cross-sectional study included 3,584 women attending population-based breast cancer screening programs and recruited between October 7, 2007, and July 14, 2008 (participation rate 74.5%). Collected data included anthropometric measurements; demographic, obstetric, and gynecologic characteristics; family and personal health history; and diet in the preceding year. Mammographic density was blindly assessed by a single radiologist and classified into four categories: less than 10%, 10-25%, 25-50%, and greater than 50%. The association between adherence to either a Western or a Mediterranean dietary pattern and mammographic density was explored using multivariable ordinal logistic regression models with random center-specific intercepts. Models were adjusted for age, body mass index, parity, menopause, smoking, family history, hormonal treatment, and calorie and alcohol intake. Differences according to women's characteristics were tested including interaction terms. RESULTS: Women with a higher adherence to the Western dietary pattern were more likely to have high mammographic density (n=242 [27%]) than women with low adherence (n=169 [19%]) with a fully adjusted odds ratio (ORQ4vsQ1) of 1.25 (95% confidence interval [CI] 1.03-1.52). This association was confined to overweight-obese women (adjusted ORQ4vsQ1 [95% CI] 1.41 [1.13-1.76]). No association between Mediterranean dietary pattern and mammographic density was observed. CONCLUSION: The Western dietary pattern was associated with increased mammographic density among overweight-obese women. Our results might inform specific dietary recommendations for women with high mammographic density.S

CURSO BÁSICO DE LIBRAS COM INSTRUTORES SURDOS: Um caminho para transformações sociais

Diante de um contexto educacional de dificuldades na escolarização, alguns surdos conseguem desenvolver a Língua de Sinais em um aspecto mais amplo, alcançando níveis de conhecimento que os possibilita se tornar instrutores de Libras, tornando-se aptos a ministrar cursos para ouvintes. Nesse sentido, o objetivo deste trabalho é relatar e refletir sobre as experiências vivenciadas com instrutores surdos durante um Curso Básico de Libras. O curso foi desenvolvido no período de fevereiro a novembro de 2019, o qual foi composto por: aulas diferenciadas, aulas de campo, avaliações, oficinas em escolas e peças teatrais. Com a ocorrência deste, pôde-se experimentar o quão importante e necessário é o estudo da Língua Brasileira de Sinais para a inclusão

Serological Proteomic Screening and Evaluation of a Recombinant Egg Antigen for the Diagnosis of Low-Intensity \u3ci\u3eSchistosoma mansoni\u3c/i\u3e infections in endemic area in Brazil

Background Despite decades of use of control programs, schistosomiasis remains a global public health problem. To further reduce prevalence and intensity of infection, or to achieve the goal of elimination in low-endemic areas, there needs to be better diagnostic tools to detect low-intensity infections in low-endemic areas in Brazil. The rationale for development of new diagnostic tools is that the current standard test Kato-Katz (KK) is not sensitive enough to detect low-intensity infections in low-endemic areas. In order to develop new diagnostic tools, we employed a proteomics approach to identify biomarkers associated with schistosome-specific immune responses in hopes of developing sensitive and specific new methods for immunodiagnosis. Methods and findings Immunoproteomic analyses were performed on egg extracts of Schistosoma mansoni using pooled sera from infected or non-infected individuals from a low-endemic area of Brazil. Cross reactivity with other soil-transmitted helminths (STH) was determined using pooled sera from individuals uniquely infected with different helminths. Using this approach, we identified 23 targets recognized by schistosome acute and chronic sera samples. To identify immunoreactive targets that were likely glycan epitopes, we compared these targets to the immunoreactivity of spots treated with sodium metaperiodate oxidation of egg extract. This treatment yielded 12/23 spots maintaining immunoreactivity, suggesting that they were protein epitopes. From these 12 spots, 11 spots cross-reacted with sera from individuals infected with other STH and 10 spots cross-reacted with the negative control group. Spot number 5 was exclusively immunoreactive with sera from S. mansoni-infected groups in native and deglycosylated conditions and corresponds to Major Egg Antigen (MEA). We expressed MEA as a recombinant protein and showed a similar recognition pattern to that of the native protein via western blot. IgG-ELISA gave a sensitivity of 87.10% and specificity of 89.09% represented by area under the ROC curve of 0.95. IgG-ELISA performed better than the conventional KK (2 slides), identifying 56/64 cases harboring 1–10 eggs per gram of feces that were undiagnosed by KK parasitological technique. Conclusions The serological proteome approach was able to identify a new diagnostic candidate. The recombinant egg antigen provided good performance in IgG-ELISA to detect individuals with extreme low-intensity infections (1 egg per gram of feces). Therefore, the IgG-ELISA using this newly identified recombinant MEA can be a useful tool combined with other techniques in low-endemic areas to determine the true prevalence of schistosome infection that is underestimated by the KK method. Further, to overcome the complexity of ELISA in the field, a second generation of antibody-based rapid diagnostic tests (RDT) can be developed

Prevalência de ovos de Helmintos nas águas residuárias urbanas na cidade de Campina Grande - PB / Prevalence of helminth eggs in urban wastewater in the city of Campina Grande - PB

Este trabalho teve por objetivo avaliar a recuperação e a frequência de ovos de helmintos em amostras de águas residuárias urbanas, considerando-se as condições sanitárias, higiênicas e sociais de populações de baixa renda. Foram avaliados os valores médios obtidos entre os bairros de baixa renda e os demais bairros da cidade de Campina Grande, Paraíba. O estudo foi desenvolvido no sistema de esgotamento sanitário e envolveu quatro pontos de coleta. Os pontos P1, P2 e P3, correspondentes aos bairros da rede geral da cidade, apresentaram menores concentrações de ovos de helmintos quando comparados ao ponto P4, correspondente aos bairros de baixa renda. As elevadas concentrações de ovos de helmintos podem ser relacionadas ao baixo nível social, econômico e de educação sanitária dos bairros de baixa renda, tendo em vista que a população dispõe de infraestrutura completa de saneamento básico

Congreso online: nueva herramienta para fomentar el aprendizaje

Los congresos científicos son una herramienta valiosa en el aprendizaje para estudiantes de tercer ciclo. Sin embargo, no son aprovechados al máximo con esta finalidad, puesto que las intervenciones por parte de los estudiantes, en cuanto a plantear cuestiones se refiere, son prácticamente nulas. Con el objetivo de fomentar la participación de los estudiantes de tercer ciclo en los congresos científicos, se presenta una propuesta de congreso online para estudiantes de doctorado en el programa interuniversitario `Electroquímica. Ciencia y Tecnología`, empleando la herramienta Moodle. Este congreso consiste en dar a conocer, de forma visible y con formato de pósters, comunicaciones científicas de los estudiantes, quienes deben hacer preguntas acerca de las mismas y responder a las realizadas sobre su contribución. Además, y siempre con la finalidad de fomentar la participación en forma de preguntas y discusiones científicas, se otorgarán premios tales como al estudiante más participativo y al mejor póster, para lo que se evaluará la defensa del póster por parte del estudiante