Gastropods from the Rockshelter Deodoro Roca (Sector B), Ongamira Valley (Cordoba, Argentina): A preliminary analysis

Se presentan los primeros resultados de la caracterización taxonómica y la cuantificación de gasterópodos procedentes de distintas unidades estratigráficas asociadas con las ocupaciones fechadas ca. 3000 AP. del Sector B del sitio ADR. El alero en cuestión había sido excavado en las décadas de 1940 y 1950, oportunidad en la que se obtuvieron gran cantidad de restos faunísticos incluidos camélidos, cérvidos, rheidos y gasterópodos (Menghin y González 1954, Pascual 1954). En 2010 se realizó la excavación de algunos sectores trabajados en la década de 1950 dejando los antiguos perfiles expuestos y la excavación de áreas no trabajadas previamente, lo que permitió identificar la presencia de fogones estructurados, con la particularidad de estar asociados a espesas capas de valvas de gasterópodos donde fueron recuperadas todas las valvas enteras y se realizó un muestreo para su cuantificación en cinco sectores del sitio. El análisis de estas valvas permitió la determinación de seis especies: cinco vivientes y una aparentemente extinta en la región; a partir de lo cual se explora su recurrencia en los sitios arqueológicos de la región, así como su potencial para la subsistencia de los grupos que habitaron el alero en el Holoceno Tardío. La cuantificación permitió interpretar que ca. 3000AP la biomasa disponible de gasterópodos terrestres en el área era muy elevada, por lo cual puede haber resultado de interés para la incorporación a la dieta prehispánica.This paper presents the first results of the taxonomic characterization and the quantification of gastropods from the Sector B of the Deodoro Roca rockshelter, Ongamira (Ischilín, Córdoba), in order to understand their role in pre-Hispanic groups subsistence strategy focusing in occupation ca. 3000bp. The site was firstly excavated in the 1940s and 1950s at which time it was obtained many faunal remains from camelids, deers, rheas and gastropods. In 2010 we proceeded to re-excavate some sectors worked in the 1950´s leaving exposed profiles and excavate other not previously worked; thus we were able to identify the presence of different spaces with structured hearths which had the distinction of being associated with thick layers of gastropods shells. The analysis of these valves allowed the determination of six species, including five that live currently in the region and other that nowadays it is apparently extinct. From this we explore the recurrence of these animals in archaeological sites in the region and its potential for the understanding of the subsistence of the groups that inhabited the rockshelter during the Late Holocene.Fil: Izeta, Andres Dario. Universidad Nacional de Cordoba. Facultad de Filosofia y Humanidades. Museo de Antropologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Silva Ferreira Da Costa, Thiago. Universidad Nacional de Cordoba. Facultad de Filosofia y Humanidades. Museo de Antropologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gordillo, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Ciencias de la Tierra; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Cattaneo, Gabriela Roxana. Universidad Nacional de Cordoba. Facultad de Filosofia y Humanidades. Museo de Antropologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Boretto, Gabriella Margherita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Ciencias de la Tierra; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Robledo, Andrés Ignacio. Universidad Nacional de Cordoba. Facultad de Filosofia y Humanidades. Museo de Antropologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Non-intubated VATS for lung cancer—a focus on segmentectomy: a narrative review

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Ornamental beads in a hunter-gatherers context from the Córdoba Province: a mineralogical and microstructural analysis of a “Borus” shell

El objetivo de este trabajo es analizar la composicion mineralogica y la microestructura del gasteropodo “Borus” (Megalobulimus lorentzianus) que habita en la provincia de Cordoba y compararla con una cuenta ornamental procedente de un sitio arqueológico de cazadores-recolectores del noroeste de la misma provincia y de esta manera contar con nuevas herramientas para identificar las materias primas utilizadas en su fabricacion. El analisis de difraccion de rayos X en base a un ejemplar actual indico que la concha de “Borus” esta compuesta en su totalidad de aragonita, polimorfo de carbonato de calcio (CaCO3) que cristaliza en el sistema ortorrombico. Ademas, el analisis de su microestructura, a traves de microscopia electronica y laser confocal, mostro una disposicion prismatica de los cristales. Por otro lado, este ultimo análisis realizado en una cuenta proveniente del sitio arqueologico Alero Deodoro Roca (valle de Ongamira, provincia de Cordoba) tambien indico la misma composicion mineralógica y disposicion prismatica, lo que se interpreta como un elemento de afiliacion al taxón mencionado. Con la incorporacion de nuevos materiales este tipo de analisis podra, a futuro, mejorar el conocimiento respecto a la especializacion en la confeccion de cuentas y posibles redes de intercambio en la region central de Argentina.In this article, we analyze the mineralogical composition and microstructure of the “Borus” gastropod (Megalobulimus lorentzianus), which is local to the Cordoba Province. The gastropod was compared against a typical ornamental bead from a hunter-gatherers archaeological site located in the northwest region of the same province, with the aim of producing new tools to identify the raw materials used in bead manufacture. An X-ray diffraction analysis of a modern “Borus” shell specimen indicated that from a mineralogical standpoint it was composed entirely of aragonite, a calcium carbonate polymorph (CaCO3) that crystallizes in the orthorhombic system. In addition, an analysis of its microstructure using laser electron confocal microscope identified a prismatic microstructure. These same analyses, undertaken on a bead from the archaeological site of Alero Deodoro Roca (Ongamira valley, Cordoba Province) revealed the same mineralogical composition and prismatic arrangement, leading to interpret the bead as having a taxonomic affiliation to “Borus” shell. With the addition of new materials, in the future this type of analysis will provide insights regarding specialization in the elaboration of beads and exchange networks in the central region of Argentina.Fil: Boretto, Gabriella Margherita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Gordillo, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Izeta, Andres Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Antropología de Córdoba. Universidad Nacional de Córdoba. Facultad de Filosofía y Humanidades. Instituto de Antropología de Córdoba; ArgentinaFil: Colombo, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Martinelli, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Cattaneo, Gabriela Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Antropología de Córdoba. Universidad Nacional de Córdoba. Facultad de Filosofía y Humanidades. Instituto de Antropología de Córdoba; Argentin

VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells.

Endothelial colony forming cells (ECFCs) represent a population of truly endothelial precursors that promote the angiogenic switch in solid tumors, such as breast cancer (BC). The intracellular Ca2+ toolkit, which drives the pro-angiogenic response to VEGF, is remodelled in tumor-associated ECFCs such that they are seemingly insensitive to this growth factor. This feature could underlie the relative failure of anti-VEGF therapies in cancer patients. Herein, we investigated whether and how VEGF uses Ca2+ signalling to control angiogenesis in BC-derived ECFCs (BCECFCs). Although VEGFR-2 was normally expressed, VEGF failed to induce proliferation and in vitro tubulogenesis in BC-ECFCs. Likewise, VEGF did not trigger robust Ca2+ oscillations in these cells. Similar to normal cells, VEGF-induced intracellular Ca2+ oscillations were triggered by inositol-1,4,5-trisphosphate-dependent Ca2+ release from the endoplasmic reticulum (ER) and maintained by store-operated Ca2+ entry (SOCE). However, InsP3-dependent Ca2+ release was significantly lower in BC-ECFCs due to the down-regulation of ER Ca2+ levels, while there was no remarkable difference in the amplitude, pharmacological profile and molecular composition of SOCE. Thus, the attenuation of the pro-angiogenic Ca2+ response to VEGF was seemingly due to the reduction in ER Ca2+ concentration, which prevents VEGF from triggering robust intracellular Ca2+ oscillations. However, the pharmacological inhibition of SOCE prevented BC-ECFC proliferation and in vitro tubulogenesis. These findings demonstrate for the first time that BC-ECFCs are insensitive to VEGF, which might explain at cellular and molecular levels the failure of anti-VEGF therapies in BC patients, and hint at SOCE as a novel molecular target for this disease

Systematic review and meta-analysis of in vitro efficacy of antibiotic combination therapy against carbapenem-resistant Gram-negative bacilli.

The superiority of combination therapy for carbapenem-resistant Gram-negative bacilli (CR-GNB) infections remains controversial. In vitro models may predict the efficacy of antibiotic regimens against CR-GNB. A systematic review and meta-analysis was performed including pharmacokinetic/pharmacodynamic (PK/PD) and time-kill (TK) studies examining the in vitro efficacy of antibiotic combinations against CR-GNB [PROSPERO registration no. CRD42019128104]. The primary outcome was in vitro synergy based on the effect size (ES): high, ES ≥ 0.75, moderate, 0.35ES0.75; low, ES ≤ 0.35; and absent, ES = 0). A network meta-analysis assessed the bactericidal effect and re-growth rate (secondary outcomes). An adapted version of the ToxRTool was used for risk-of-bias assessment. Over 180 combination regimens from 136 studies were included. The most frequently analysed classes were polymyxins and carbapenems. Limited data were available for ceftazidime/avibactam, ceftolozane/tazobactam and imipenem/relebactam. High or moderate synergism was shown for polymyxin/rifampicin against Acinetobacter baumannii [ES = 0.91, 95% confidence interval (CI) 0.44-1.00], polymyxin/fosfomycin against Klebsiella pneumoniae (ES = 1.00, 95% CI 0.66-1.00) and imipenem/amikacin against Pseudomonas aeruginosa (ES = 1.00, 95% CI 0.21-1.00). Compared with monotherapy, increased bactericidal activity and lower re-growth rates were reported for colistin/fosfomycin and polymyxin/rifampicin in K. pneumoniae and for imipenem/amikacin or imipenem/tobramycin against P. aeruginosa. High quality was documented for 65% and 53% of PK/PD and TK studies, respectively. Well-designed in vitro studies should be encouraged to guide the selection of combination therapies in clinical trials and to improve the armamentarium against carbapenem-resistant bacteria

Editing of HIV-1 RNA by the double-stranded RNA deaminase ADAR1 stimulates viral infection

Adenosine deaminases that act on dsRNA (ADARs) are enzymes that target double-stranded regions of RNA converting adenosines into inosines (A-to-I editing) thus contributing to genome complexity and fine regulation of gene expression. It has been described that a member of the ADAR family, ADAR1, can target viruses and affect their replication process. Here we report evidence showing that ADAR1 stimulates human immuno deficiency virus type 1 (HIV-1) replication by using both editing-dependent and editing-independent mechanisms. We show that over-expression of ADAR1 in HIV-1 producer cells increases viral protein accumulation in an editing-independent manner. Moreover, HIV-1 virions generated in the presence of over-expressed ADAR1 but not an editing-inactive ADAR1 mutant are released more efficiently and display enhanced infectivity, as demonstrated by challenge assays performed with T cell lines and primary CD4+ T lymphocytes. Finally, we report that ADAR1 associates with HIV-1 RNAs and edits adenosines in the 5′ untranslated region (UTR) and the Rev and Tat coding sequence. Overall these results suggest that HIV-1 has evolved mechanisms to take advantage of specific RNA editing activity of the host cell and disclose a stimulatory function of ADAR1 in the spread of HIV-1

The Health Effects of Climate Change: A Survey of Recent Quantitative Research

In recent years there has been a large scientific and public debate on climate change and its direct as well as indirect effects on human health. In particular, a large amount of research on the effects of climate changes on human health has addressed two fundamental questions. First, can historical data be of some help in revealing how short-run or long-run climate variations affect the occurrence of infectious diseases? Second, is it possible to build more accurate quantitative models which are capable of predicting the future effects of different climate conditions on the transmissibility of particularly dangerous infectious diseases? The primary goal of this paper is to review the most relevant contributions which have directly tackled those questions, both with respect to the effects of climate changes on the diffusion of non-infectious and infectious diseases, with malaria as a case study. Specific attention will be drawn on the methodological aspects of each study, which will be classified according to the type of quantitative model considered, namely time series models, panel data and spatial models, and non-statistical approaches. Since many different disciplines and approaches are involved, a broader view is necessary in order to provide a better understanding of the interactions between climate and health. In this respect, our paper also presents a critical summary of the recent literature related to more general aspects of the impacts of climate changes on human health, such as: the economics of climate change; how to manage the health effects of climate change; the establishment of Early Warning Systems for infectious diseases

Clinical characteristics and risk factors associated with COVID-19 severity in patients with haematological malignancies in Italy: a retrospective, multicentre, cohort study

Several small studies on patients with COVID-19 and haematological malignancies are available showing a high mortality in this population. The Italian Hematology Alliance on COVID-19 aimed to collect data from adult patients with haematological malignancies who required hospitalisation for COVID-19

New records of rare species in the Mediterranean Sea (October 2020)

This Collective Article presents information about 21 taxa belonging to seven Phyla (one Ochrophyta, one Porifera, three Cnidaria, two Arthropoda, three Mollusca, one Echinodermata, and ten Chordata) and extending from the western Mediterranean Sea to the Levantine Sea. The new records were reported from nine countries as follows: Spain: first records of three deep-sea species from the Blanes Canyon along the Catalan margin, namely the gorgonian Placogorgia coronata, the bivalve Acesta excavata, and the Azores rockling Gaidropsarus granti; Italy: first record of the mesopsammic nudibranch Embletonia pulchra from Ligurian shallow-waters; first record of the deep-sea carnivorous sponge Lycopodina hypogea from the north-central Tyrrhenian Sea, living in dense clusters over dead black corals; new records of the Portuguese man o’ war Physalia physalis from Sardinian and Sicilian waters; first Italian record of the large asteroid Coronaster briareus from the Ionian Sea; first record of the white grouper Epinephelus aeneus in the northernmost point of the Adriatic Sea; Croatia: first record of the gastropod Haliotis mykonosensis for the Adriatic Sea; Malta: new sightings of Physalia physalis from Maltese waters; Libya: first record of the sand crab Albunea carabus from two localities along the Libyan coast; Greece: first records of the deep-sea black coral Parantipathes larix from the eastern Mediterranean Sea; first verified record of the agujon needlefish Tylosurus imperialis in the Hellenic Ionian Sea; first confirmed record of the brown algae Treptacantha squarrosa in the eastern Mediterranean Sea; new records of three deep-sea fish species from the Aegean Sea, namely the bluntnose sixgill shark Hexanchus griseus, the Atlantic pomfret Brama brama, and the rudderfish Centrolophus niger; new record of the tripletail Lobotes surinamensis from Lesvos Island; new record of the shrimp Brachycarpus biunguiculatus from the gut content of the non-indigenous lionfish Pterois miles; Turkey: new record of the imperi- al blackfish Schedophilus ovalis from Turkish waters; Lebanon: first record of the slender sunfish Ranzania laevis, stranded along the Lebanese coast; Israel: new record, after about 60 years from the last catch, of the spotted dragonet Callionymus maculatus.peer-reviewe

Antidiabetic Drug Prescription Pattern in Hospitalized Older Patients with Diabetes

Objective: To describe the prescription pattern of antidiabetic and cardiovascular drugs in a cohort of hospitalized older patients with diabetes. Methods: Patients with diabetes aged 65 years or older hospitalized in internal medicine and/or geriatric wards throughout Italy and enrolled in the REPOSI (REgistro POliterapuie SIMI—Società Italiana di Medicina Interna) registry from 2010 to 2019 and discharged alive were included. Results: Among 1703 patients with diabetes, 1433 (84.2%) were on treatment with at least one antidiabetic drug at hospital admission, mainly prescribed as monotherapy with insulin (28.3%) or metformin (19.2%). The proportion of treated patients decreased at discharge (N = 1309, 76.9%), with a significant reduction over time. Among those prescribed, the proportion of those with insulin alone increased over time (p = 0.0066), while the proportion of those prescribed sulfonylureas decreased (p < 0.0001). Among patients receiving antidiabetic therapy at discharge, 1063 (81.2%) were also prescribed cardiovascular drugs, mainly with an antihypertensive drug alone or in combination (N = 777, 73.1%). Conclusion: The management of older patients with diabetes in a hospital setting is often sub-optimal, as shown by the increasing trend in insulin at discharge, even if an overall improvement has been highlighted by the prevalent decrease in sulfonylureas prescription