Exotic looped trajectories via quantum marking

We provide an analytical and theoretical study of exotic looped trajectories (ELTs) in a double-slit interferometer with quantum marking. We use an excited Rydberg-like atom and which-way detectors such as superconducting cavities, just as in the Scully-Englert-Walther interferometer. We indicate appropriate conditions on the atomic beam or superconducting cavities so that we determine an interference pattern and fringe visibility exclusive from the ELTs. We quantitatively describe our results for Rubidium atoms and propose this framework as an alternative scheme to the double-slit experiment modified to interfere only these exotic trajectories.Comment: 10 pages, 5 figure

A REALIDADE DO SISTEMA PÚBLICO DE ASSISTENCIA AO IDOSO: UM PANORAMA PSICOSSOCIAL DA REGIÃO METROPOLITANA DE BRASÍLIA

The care service to the elderly community in the metropolitan region of Ceilândia has been the subject of social discussions about the precariousness and lack of public resources to meet the region's quantity. In this scenario, we investigated the reality of long-term public and private institutions and religious institutes, in order to verify the preventive and palliative actions of the physical, psychic, social and emotional care system. It was observed that the elderly of long-term services in public institutions had contradictory feelings of well-being and psychological distress, to the detriment of the feeling of loneliness and abandonment by the absence of family in their daily lives and precariousness of the services offered. In contrast, the elderly in private and religious institutions verbalize the feeling of emptiness caused by the daily living of family members, but the recreational and social activities keep them alive and emotionally stable. This work is relevant for the elderly community in understanding social bottlenecks, within the legal right of the elderly, in guaranteeing rights to housing, food and housing. The objective was to present an overview of the practical perspective of the actions of welcoming, recreation and rest of the elderly in situations of vulnerability and social risk in the institutional context, since family abandonment is one of the causes that affect physical and psychosocial health, triggering diseases. like anxiety and depression. From the social point of view, the discussion of public social assistance policies regarding the care service to the elderly community is fundamental, given that the Brazilian population is aging, and this theme has been neglected by the responsible public health agencies. . For the scientific community, the study is significant as a guide for future research in the social area. It is a descriptive and exploratory research about the investigated field.El servicio de atención a la comunidad de ancianos en la región metropolitana de Ceilândia ha sido objeto de debates sociales sobre la precariedad y la falta de recursos públicos para satisfacer la cantidad de la región. En este escenario, investigamos la realidad de las instituciones públicas y privadas e institutos religiosos a largo plazo, a fin de verificar las acciones preventivas y paliativas del sistema de atención física, psíquica, social y emocional. Se observó que los ancianos de servicios a largo plazo en instituciones públicas tenían sentimientos contradictorios de bienestar y angustia psicológica, en detrimento del sentimiento de soledad y abandono por la ausencia de la familia en su vida cotidiana y la precariedad de los servicios ofrecidos. En contraste, los ancianos en instituciones privadas y religiosas verbalizan el sentimiento de vacío causado por la vida diaria de los miembros de la familia, pero las actividades recreativas y sociales los mantienen vivos y emocionalmente estables. Este trabajo es relevante para la comunidad de ancianos en la comprensión de los cuellos de botella sociales, dentro del derecho legal de los ancianos, en la garantía de los derechos a la vivienda, la alimentación y la vivienda. El objetivo fue presentar una visión general de la perspectiva práctica de las acciones de acogida, recreación y descanso de las personas mayores en situaciones de vulnerabilidad y riesgo social en el contexto institucional, ya que el abandono familiar es una de las causas que afectan la salud física y psicosocial, desencadenando enfermedades. como ansiedad y depresión. Desde el punto de vista social, la discusión de las políticas públicas de asistencia social con respecto al servicio de atención a la comunidad de ancianos es fundamental, dado que la población brasileña está envejeciendo, y este tema ha sido descuidado por las agencias responsables de salud pública. . Para la comunidad científica, el estudio es significativo como guía para futuras investigaciones en el área social. Es una investigación descriptiva y exploratoria sobre el campo investigado.O serviço de atendimento à comunidade de idosos na região metropolitana de Ceilândia tem sido pauta de discussões sociais sobre a precariedade e falta de recursos públicos para atender o quantitativo da região. Neste panorama, investigou-se a realidade das instituições de longa permanência, de natureza pública, privada e institutos religiosos, a fim de verificar as ações preventivas e paliativas do sistema de atendimento físico, psíquico, social e emocional. Observou- se que os idosos dos serviços de longa permanência em instituições públicas apresentaram sentimentos contraditórios de bem-estar e sofrimento psíquico, em detrimento do sentimento de solidão e abandono pela ausência familiar em seu cotidiano e precariedade dos serviços ofertados. Em contrapartida, os idosos de instituições privadas e religiosas verbalizam o sentimento de vazio ocasionado pela convivência diária dos familiares, contudo as atividades recreativas e sociais os mantém vivos e emocionalmente estável. Este trabalho é relevante para a comunidade idosa na compreensão dos gargalos sociais, no âmbito do direito legal do idoso, na garantia de direitos à moradia, alimentação e habitação. Objetivou-se apresentar um panorama da perspectiva prática das ações de acolhimento, recreação e repouso de idosos em situações de vulnerabilidade e risco social no contexto institucional, uma vez que o abandono familiar é uma das causas que afetam a saúde física e psicossocial, desencadeando doenças como ansiedade e depressão. Sob a ótica social, é fundamental a discussão das políticas públicas de assistência social, no que tange o serviço de atendimento à comunidade de idosos, tendo em vista que a população Brasileira está envelhecendo, e este tema tem sido negligenciado pelos órgãos responsáveis de saúde pública. Para a comunidade cientifica, o estudo é significativo como norteador de futuras pesquisas na área social. Trata-se de uma pesquisa descritiva e exploratória acerca do campo investigado

Essential role of the czc determinant for cadmium, cobalt and zinc resistance in Gluconacetobacter diazotrophicus PAl 5

The mechanisms of cadmium, cobalt and zinc resistance were characterized in the plant-growth-promoting bacterium Gluconacetobacter diazotrophicus PAl 5. The resistance level of the wild-type strain was evaluated through the establishment of minimum inhibitory concentrations (MIC) of the soluble compounds CdCl2·H2O, CoCl2·6H2O and ZnCl2. Gluconacetobacter diazotrophicus PAl 5 was resistant to high concentrations of Cd, Co and Zn, with MICs of 1.2, 20 and 20 mM, respectively. Screening of an insertion library from transposon EZ-Tn5<R6Kyori/KAN-2> in the presence of ZnO revealed that the mutant GDP30H3 was unable to grow in the presence of the compound. This mutant was also highly sensitive to CdCl2·H2O, CoCl2·6H2O and ZnCl2. Molecular characterization established that the mutation affected the czcA gene, which encodes a protein involved in metal efflux. In silico analysis showed that czcA is a component of the czcCBARS operon together with four other genes. This work provides evidence of the high tolerance of G. diazotrophicus PAl 5 to heavy metalsand that czc is a determinant for metal resistance in this bacterium. [Int Microbiol 2012; 15(2):69-78

Analysis of the biofilm proteome of Xylella fastidiosa

<p>Abstract</p> <p>Background</p> <p><it>Xylella fastidiosa </it>is limited to the xylem of the plant host and the foregut of insect vectors (sharpshooters). The mechanism of pathogenicity of this bacterium differs from other plant pathogens, since it does not present typical genes that confer specific interactions between plant and pathogens (avr and/or hrp). The bacterium is injected directly into the xylem vessels where it adheres and colonizes. The whole process leads to the formation of biofilms, which are considered the main mechanism of pathogenicity. Cells in biofilms are metabolically and phenotypically different from their planktonic condition. The mature biofilm stage (phase of higher cell density) presents high virulence and resistance to toxic substances such as antibiotics and detergents. Here we performed proteomic analysis of proteins expressed exclusively in the mature biofilm of <it>X. fastidiosa </it>strain 9a5c, in comparison to planktonic growth condition.</p> <p>Results</p> <p>We found a total of 456 proteins expressed in the biofilm condition, which correspond to approximately 10% of total protein in the genome. The biofilm showed 37% (or 144 proteins) different protein than we found in the planktonic growth condition. The large difference in protein pattern in the biofilm condition may be responsible for the physiological changes of the cells in the biofilm of <it>X. fastidiosa</it>. Mass spectrometry was used to identify these proteins, while real-time quantitative polymerase chain reaction monitored expression of genes encoding them. Most of proteins expressed in the mature biofilm growth were associated with metabolism, adhesion, pathogenicity and stress conditions. Even though the biofilm cells in this work were not submitted to any stress condition, some stress related proteins were expressed only in the biofilm condition, suggesting that the biofilm cells would constitutively express proteins in different adverse environments.</p> <p>Conclusions</p> <p>We observed overexpression of proteins related to quorum sensing, proving the existence of communication between cells, and thus the development of structuring the biofilm (mature biofilm) leading to obstruction of vessels and development of disease. This paper reports a first proteomic analysis of mature biofilm of <it>X. fastidiosa</it>, opening new perspectives for understanding the biochemistry of mature biofilm growth in a plant pathogen.</p

The role of retailer's performance in optimal wholesale price discount policies

The main goal of this paper is to model the effects of wholesale price control on manufacturer’s profit, taking explicitly into account the retailer’s sales motivation and performance. We consider a stylized distribution channel where a manufacturer sells a single kind of good to a single retailer. Wholesale price discounts are assumed to increase the retailer’s motivation thus improving sales. We study the manufacturer’s profit maximization problem as an optimal control model where the manufacturer’s control is the discount on wholesale price and retailer’s motivation is one of the state variables. In particular in the paper we prove that an increasing discount policy is optimal for the manufacturer when the retailer is not efficient while efficient retailers may require to decrease the trade discounts at the end of the selling period. Computational experiments point out how the discount on wholesale price passed by the retailer to the market (passthrough) influences the optimal profit of the manufacturer

Rational Design and Synthesis of Large Stokes Shift 2,6-Sulphur-Disubstituted BODIPYs for Cell Imaging

Five new disubstituted 2,6-thioaryl-BODIPY dyes were synthesized via selective aromatic electrophilic substitution from commercially available thiophenols. The analysis of the photophysical properties via absorption and emission spectroscopy showed unusually large Stokes shifts for BODIPY fluorophores (70&ndash;100 nm), which makes them suitable probes for bioimaging. Selected compounds were evaluated for labelling primary immune cells as well as different cancer cell lines using confocal fluorescence microscopy

Characterization of indicator tree species in neotropical environments and implications for geological mapping

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOGeobotanical remote sensing (GbRS) in the strict sense is an indirect approach to obtain geological information in heavily vegetated areas for mineral prospecting and geological mapping. Using ultra- and hyperspectral technologies, the goals of this resea216385400FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO2010/51758-2, 2010/51718-0309712/2017-3, 302925/2015-

Optimizing exosomal RNA isolation for RNA-Seq analyses of archival sera specimens

Exosomes are endosome-derived membrane vesicles that contain proteins, lipids, and nucleic acids. The exosomal transcriptome mediates intercellular communication, and represents an understudied reservoir of novel biomarkers for human diseases. Next-generation sequencing enables complex quantitative characterization of exosomal RNAs from diverse sources. However, detailed protocols describing exosome purification for preparation of exosomal RNA-sequence (RNA-Seq) libraries are lacking. Here we compared methods for isolation of exosomes and extraction of exosomal RNA from human cell-free serum, as well as strategies for attaining equal representation of samples within pooled RNA-Seq libraries. We compared commercial precipitation with ultracentrifugation for exosome purification and confirmed the presence of exosomes via both transmission electron microscopy and immunoblotting. Exosomal RNA extraction was compared using four different RNA purification methods. We determined the minimal starting volume of serum required for exosome preparation and showed that high quality exosomal RNA can be isolated from sera stored for over a decade. Finally, RNA-Seq libraries were successfully prepared with exosomal RNAs extracted from human cell-free serum, cataloguing both coding and non-coding exosomal transcripts. This method provides researchers with strategic options to prepare RNA-Seq libraries and compare RNA-Seq data quantitatively from minimal volumes of fresh and archival human cell-free serum for disease biomarker discovery