Modelling soil water conent in a tomato field: proximal gamma ray spectroscopy and soil-crop system models

Proximal soil sensors are taking hold in the understanding of soil hydrogeological processes involved in precision agriculture. In this context, permanently installed gamma ray spectroscopy stations represent one of the best space-time trade off methods at field scale. This study proved the feasibility and reliability of soil water content monitoring through a seven-month continuous acquisition of terrestrial gamma radiation in a tomato test field. By employing a 1 L sodium iodide detector placed at a height of 2.25 m, we investigated the gamma signal coming from an area having a ~25 m radius and from a depth of approximately 30 cm. Experimental values, inferred after a calibration measurement and corrected for the presence of biomass, were corroborated with gravimetric data acquired under different soil moisture conditions, giving an average absolute discrepancy of about 2%. A quantitative comparison was carried out with data simulated by AquaCrop, CRITeRIA, and IRRINET soil-crop system models. The different goodness of fit obtained in bare soil condition and during the vegetated period highlighted that CRITeRIA showed the best agreement with the experimental data over the entire data-taking period while, in presence of the tomato crop, IRRINET provided the best results.Comment: 18 pages, 9 Figures, 3 Table

Transcriptome-wide expression profiling in skin fibroblasts of patients with joint hypermobility syndrome/ehlers-danlos syndrome hypermobility type

Joint hypermobility syndrome/Ehlers-Danlos syndrome hypermobility type (JHS/EDS-HT), is likely the most common systemic heritable connective tissue disorder, and is mostly recognized by generalized joint hypermobility, joint instability complications, minor skin changes and a wide range of satellite features. JHS/EDS-HT is considered an autosomal dominant trait but is still without a defined molecular basis. The absence of (a) causative gene(s) for JHS/EDS-HT is likely attributable to marked genetic heterogeneity and/or interaction of multiple loci. In order to help in deciphering such a complex molecular background, we carried out a comprehensive immunofluorescence analysis and gene expression profiling in cultured skin fibroblasts from five women affected with JHS/EDS-HT. Protein study revealed disarray of several matrix structural components such as fibrillins, tenascins, elastin, collagens, fibronectin, and their integrin receptors. Transcriptome analysis indicated perturbation of different signaling cascades that are required for homeostatic regulation either during development or in adult tissues as well as altered expression of several genes involved in maintenance of extracellular matrix architecture and homeostasis (e.g., SPON2, TGM2, MMP16, GPC4, SULF1), cell-cell adhesion (e.g., CDH2, CHD10, PCDH9, CLDN11, FLG, DSP), immune/inflammatory/pain responses (e.g., CFD, AQP9, COLEC12, KCNQ5, PRLR), and essential for redox balance (e.g., ADH1C, AKR1C2, AKR1C3, MAOB, GSTM5). Our findings provide a picture of the gene expression profile and dysregulated pathways in JHS/EDS-HT skin fibroblasts that correlate well with the systemic phenotype of the patients

GLUT10 deficiency leads to oxidative stress and non-canonical αvβ3 integrin-mediated TGFβ signalling associated with extracellular matrix disarray in arterial tortuosity syndrome skin fibroblasts

Arterial tortuosity syndrome (ATS) is an autosomal recessive connective tissue disorder caused by loss-of-function mutations in SLC2A10, which encodes facilitative glucose transporter 10 (GLUT10). The role of GLUT10 in ATS pathogenesis remains an enigma, and the transported metabolite(s), i.e. glucose and/or dehydroascorbic acid, have not been clearly elucidated. To discern the molecular mechanisms underlying the ATS aetiology, we performed gene expression profiling and biochemical studies on skin fibroblasts. Transcriptome analyses revealed the dysregulation of several genes involved in TGFβ signalling and extracellular matrix (ECM) homeostasis as well as the perturbation of specific pathways that control both the cell energy balance and the oxidative stress response. Biochemical and functional studies showed a marked increase in ROS-induced lipid peroxidation sustained by altered PPARγ function, which contributes to the redox imbalance and the compensatory antioxidant activity of ALDH1A1. ATS fibroblasts also showed activation of a non-canonical TGFβ signalling due to TGFBRI disorganization, the upregulation of TGFBRII and connective tissue growth factor, and the activation of the αvβ3 integrin transduction pathway, which involves p125FAK, p60Src and p38 MAPK. Stable GLUT10 expression in patients' fibroblasts normalized redox homeostasis and PPARγ activity, rescued canonical TGFβ signalling and induced partial ECM re-organization. These data add new insights into the ATS dysregulated biological pathways and definition of the pathomechanisms involved in this disorder

Mesh Fixation Methods in Groin Hernia Surgery

No unanimous consent has been reached by surgeons in terms of a method for mesh fixation in laparoscopic and open surgery for inguinal hernia repair. Many different methods of fixation are available, and the choice of which one to use is still based on surgeons’ preferences. At present, tissue glues, sutures, and laparoscopic tacks are the most common fixating methods. In open technique, sutures have been the method of choice for their reduced costs and surgeons’ habits. Nevertheless, tissue glues have been demonstrated to be effective and safe. Similarly, tacks can be considered the most common means of fixation in laparoscopic hernia repair, but they are connected to a higher risk of complication and morbidity. In this chapter, we present these types of mesh fixation, their characteristics and potential risks, and advantages of their use

All-in-One Mesh Hernioplasty: A New Procedure for Inguinal Hernia Repair

Since the 1970s, when biocompatible meshes were introduced with the consequent decrease of recurrence, one of the priorities in inguinal hernia surgery was that of minimizing postoperative chronic pain. All technical variations, proposed during the past years in order to improve patient’s comfort, reported a variable incidence of chronic neuralgia. The procedure we describe, applicable to all cases of primary inguinal hernia, employs a smaller pre-cut single mesh that covers all weak areas of the inguinal canal and is enveloped in a fibro-cremasteric sheath, avoiding contact of the prosthesis with neural structures. The new procedure, already performed on 250 patients, aims to improve patients’ comfort and to reduce the incidence of chronic neuralgia

Water retention potentials of Italian soils and physiological responses of potted golden kiwifruit

Correct water management of golden kiwifruit vines, besides being essential for reaching high yield and fruit quality, is also fundamental to keep plants healthy and avoid useless water loss. The objectives of this trial were to: 1) assess the water retention curve in typical soils for the kiwifruit production in Italy; 2) evaluate the response of potted Zezy002 plants to the variation of soil moisture, in term of leaf gas exchange and stem water potential. Plants grown on each soil were divided into two groups: 3 plants were irrigated maintaining soil water at field capacity (control); 4 plants were subjected to water stress as follows: irrigation rate was reduced 50 % of the evapotranspiration rate for one week, then irrigation was suspended. Two days after water suspension, 2 of the 4 stressed plants were irrigated as for the control plants (recovery), the other 2 vines were not irrigated (stress). Each pot was provided with a chalk potentiometric probe so that soil matric potential was constantly monitored. During the experiment leaf gas exchange and stem water potential were daily measured. After irrigation stop, a decrease of net photosynthesis, transpiration and stomatal conductance were observed, on the other hand, intercellular CO2 concentration showed the opposite trend. Specific behavior has been detected for the two loam soils tested (here labelled as “sand-clay-silt” and “silt” soils). At wilting point, established when plant photosynthetic activity stopped, soil matric potential, measured by chalk probes, ranged between -1.8 and -2.0 MPa in all soils with the exception of “sandy-clay-silt”, where it was -0.49 MPa. Soil matric potential at field water capacity ranged between -20 and -50 kPa in all soils with the exception of “silt” soil (-140 kPa). This peculiar behavior will be likely due to specific pore size distribution or poor-connected pores, however further investigations are needed

Structural insights into the inhibition of M. tuberculosis salicylate synthase (MbtI)

Tuberculosis represents the first cause of death from a single infectious agent in the world. This alarming trend is worsened by the growing emergence of resistant strains of Mycobacterium tuberculosis, which pose a serious threat to the public’s health. Therefore, the discovery of new antitubercular agents has still a critical importance. In this context, the mycobacterium-specific salicylate synthase MbtI, involved in the siderophore-mediated iron uptake pathway, has been recently validated as a pharmacological target for the development of novel antitubercular agents. A structure-based virtual screening allowed us to identify a competitive furan-based inhibitor of MbtI. With the aim of increasing its inhibitory effect, we explored the chemical space around this hit through a structure-activity relationship study, which led to the identification of more potent MbtI inhibitors. These compounds exhibited a promising antimycobacterial action, related to the reduced production of siderophores. The novel lead compounds were submitted to co-crystallization experiments to empirically define the binding mode of this class of compounds within the active site of MbtI. The structural data evidenced an unexpected binding mode, completely overturned with respect to the computational model. An investigation of the active site revealed that the absence of the magnesium ion in the crystal was likely responsible for the different pose. Therefore, we undertook a careful analysis of the role of the metal co-factor in the binding of the ligand through biochemical and structural experiments, which led to the disclosure of significant information regarding the catalytic activity of the enzyme. Our results allowed the rational modification of our scaffold, ultimately leading to the obtainment of improved compounds

A Multicenter Retrospective Survey regarding Diabetic Ketoacidosis Management in Italian Children with Type 1 Diabetes

We conducted a retrospective survey in pediatric centers belonging to the Italian Society for Pediatric Diabetology and Endocrinology. The following data were collected for all new-onset diabetes patients aged 0-18 years: DKA (pH < 7.30), severe DKA (pH < 7.1), DKA in preschool children, DKA treatment according to ISPAD protocol, type of rehydrating solution used, bicarbonates use, and amount of insulin infused. Records (n = 2453) of children with newly diagnosed diabetes were collected from 68/77 centers (87%), 39 of which are tertiary referral centers, the majority of whom (n = 1536, 89.4%) were diagnosed in the tertiary referral centers. DKA was observed in 38.5% and severe DKA in 10.3%. Considering preschool children, DKA was observed in 72%, and severe DKA in 16.7%. Cerebral edema following DKA treatment was observed in 5 (0.5%). DKA treatment according to ISPAD guidelines was adopted in 68% of the centers. In the first 2 hours, rehydration was started with normal saline in all centers, but with different amount. Bicarbonate was quite never been used. Insulin was infused starting from third hour at the rate of 0.05-0.1 U/kg/h in 72% of centers. Despite prevention campaign, DKA is still observed in Italian children at onset, with significant variability in DKA treatment, underlying the need to share guidelines among centers

How to nudge students toward healthier snacks? Consumer neuroscience insights on multisensory nudge interventions in university vending machines

Nudge has proven effective in promoting healthier eating, especially in academic environments. However, its application in vending machines has not been extensively studied yet, with existing studies focusing on choice and overlooking the emotional and cognitive responses to these interventions. Our research explored how visual and olfactory nudges (and a combination of both) can encourage healthier choices in university vending machines, and examined the related emotional and cognitive reactions, adopting a consumer neuroscience approach. It encompassed three distinct analysis levels: behavioral (snack choice), neurophysiological (emotional and cognitive reactions assessed through skin conductance and electroencephalography), and attentional (visual attention on snacks evaluated via eye-tracking). The findings revealed that while visual and olfactory nudges, when considered individually, were associated with an increased likelihood of making healthier choices, their combined effect was not significant. Skin conductance indicated that olfactory and combined nudge interventions induced relaxation, potentially promoting healthier choices. Electroencephalography results suggested a reduced motivational approach toward snacks when both nudges were combined. Eye-tracking indicated that individuals pay heightened attention to healthy snacks when positioned at eye level, however, this can also be affected by the nudge condition. Our findings suggest that implementing visual or olfactory nudges alone may promote healthier choices in vending machines, whereas combining them may lessen their impact, and corroborate the importance of positioning target products in locations where they are readily visible to consumers. These insights enhance our understanding of the emotional, cognitive, and attentional components behind nudge interventions and offer practical strategies for promoting healthier eating habits