ATENDERAH: A SERIOUS VIDEOGAME TO ENTERTAIN AND TEACH SKILLS IN ADULTS WITH ADHD

  This paper describes a video game named aTenDerAH, which was created, designed and implemented for attending the needs of adults with Attention Deficit Hyperactivity Disorder (ADHD). The purpose of aTenDerAH is entertains while different cognitive and organizational areas are being taught. aTenDerAH is integrated into an e-learning platform as a recommended tool for students with ADHD which were previously diagnosed with a user modelling process. The videogame was developed using: Unity for the creation of the 3D game and also for the interactive content, Cinema 4D for the creation of the models and animations in 3D, and Photoshop for the creation of textures for the 3D models.

Association of Hsp70 locus polymorphism with thermotolerance and ailment occurrence in Gulf Creole cattle within intensive systems

Objective: To estimate thermotolerance through the analysis of physiological constants, as well as the conditions suffered by Gulf Creole (BCG) cattle and relate it to the hsp70 locus polymorphism under an intensive production system. Design/methodology/approach: Leukocyte DNA from 60 BCG was genotyped using a 440 bp fragment by PCR-RFLP (Fok I). Physiological variables were estimated at 7-day intervals for 4 months during the hot season: respiratory rate (RF) and layer temperature (TC); likewise, the environmental variables temperature and humidity were recorded to determine thermal comfort. From the database of the production system, the conditions of the animals within the study period were quantified and categorized. Results: The hsp70 gene was polymorphic in BCG. The frequency of the AB heterozygous genotype was 0.77 and for the AA homozygous genotype it was 0.23, with a predominance of the A allele (0.61). Data analysis allowed us to find differences in RF in BCG with AA and AB genotypes (p<0.05). CT showed no differences (p>0.05). The genotype and environmental discomfort did not show an effect on the presentation of conditions in BCG (p>0.05). Limitations on study/implications: Due to the fact that the intensive system is changing and the BCG stay period is short, there are few animals that can be used for the study. Findings/conclusions: BCG is polymorphic and thermotolerant and its performance in the presentation of conditions is favorable.Objective: To estimate thermotolerance by analyzing physiological constants and ailment occurrence in Gulf Creole bovine cattle (GCB) and to relate them to Hsp70 locus polymorphism under an intensive production system. Design/Methodology/Approach: Using a 440 bp fragment, we genotyped leukocyte DNA from 60 BCG through PCR-RFLP (Fok I). Physiological variables were estimated at 7-day intervals for four months during the hottest season. The variables considered were respiratory rate (RR) and layer temperature (LT). Environmental variables—temperature and humidity— were also recorded to determine thermal comfort. Using the production system database, we categorized the animals’ ailments during the studied period. Results: The Hsp70 gene in GCB is polymorphic. The frequency of the AB heterozygous genotype was 0.77; for the AA homozygous genotype, it was 0.23. We observed a predominance of the A allele (0.61). Data analysis allowed us to find differences in RR in GCB with AA and AB genotypes (p<0.05). LT showed no differences (p>0.05). The genotype did not affect ailment occurrence in GCB (p>0.05). Study limitations/implications: Since the intensive system is dynamic, and the GCB stay period is short, few animals were available for the study. Findings/Conclusions: The Hsp70 gene present in GCB is polymorphic, and the animals are thermotolerant. Their performance regarding the occurrence of clinical conditions is favorable

Automatic Discrimination of Species within the Enterobacter cloacae Complex Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry and Supervised Algorithms

The Enterobacter cloacae complex (ECC) encompasses heterogeneous clusters of species that have been associated with nosocomial outbreaks. These species may have different acquired antimicrobial resistance and virulence mechanisms, and their identification is challenging. This study aims to develop predictive models based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) profiles and machine learning for species-level identification. A total of 219 ECC and 118 Klebsiella aerogenes clinical isolates from three hospitals were included. The capability of the proposed method to differentiate the most common ECC species (Enterobacter asburiae, Enterobacter kobei, Enterobacter hormaechei, Enterobacter roggenkampii, Enterobacter ludwigii, and Enterobacter bugandensis) and K. aerogenes was demonstrated by applying unsupervised hierarchical clustering with principal-component analysis (PCA) preprocessing. We observed a distinctive clustering of E. hormaechei and K. aerogenes and a clear trend for the rest of the ECC species to be differentiated over the development data set. Thus, we developed supervised, nonlinear predictive models (support vector machine with radial basis function and random forest). The external validation of these models with protein spectra from two participating hospitals yielded 100% correct species-level assignment for E. asburiae, E. kobei, and E. roggenkampii and between 91.2% and 98.0% for the remaining ECC species; with data analyzed in the three participating centers, the accuracy was close to 100%. Similar results were obtained with the Mass Spectrometric Identification (MSI) database developed recently (https://msi.happy-dev.fr) except in the case of E. hormaechei, which was more accurately identified with the random forest algorithm. In short, MALDI-TOF MS combined with machine learning was demonstrated to be a rapid and accurate method for the differentiation of ECC species

Epitelial mesenchymal transition : from the molecular to physiologic

La transición epitelio mesénquima (EMT) es un proceso compuesto de diferentes fases, donde una célula epitelial adquiere un fenotipo mesenquimal. Dentro de los cambios involucrados se encuentran: pérdida de la polaridad celular, adquisición de una capacidad migratoria, capacidad invasora, resistencia a la apoptosis y aumento en la producción de componentes de la matriz extracelular. Todos estos cambios ocurren como una consecuencia de la activación y represión de genes involucrados con rutas de señalización específicas relacionadas con este evento. La EMT está relacionada con procesos fisiológicos y patológicos como el cáncer. Consta de tres fases: una de células no migratorias, células premigratorias y células migratorias; cada una de ellas producto de diferentes señales intra o extracelulares, factores de transcripción (TGF-B, Snail, TWIST, Sox, Slug, ZEB1, entre otras) y proteínas involucradas (E-cadherina, integrina, vimentina, ocludinas y claudinas).Revisión1-10Transition mesenchymal epithelium (EMT) is a process composed of different phases where an epithelial cell acquires a mesenchymal phenotype. Among the changes involved are: loss of cellular polarity, acquisition of a migratory capacity, invasive capacity, resistance to apoptosis, and increase in the production of components of the extracellular matrix. All these changes occur as a consequence of the activation and repression of genes involved with specific signaling pathways related to this event. EMT is related to physiological and pathological processes such as cancer. It consists of three phases: A phase of non-migratory cells, pre-migratory cells and migratory cells; (TGF-B, Snail, TWIST, Sox, Slug, ZEB1 among others), and proteins involved (E-cadherin, integrin, vimentin, occludins and claudins)

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Semillas de vida. Agricultura, conocimiento tradicional y recursos naturales en México.

El objetivo de este libro es valorar los estudios sobre sistemas agrícolas y conocimiento tradicional de corte etnográfico. Por ello, cada capítulo aporta estudios relacionados con el conocimiento tradicional, la cosmovisión indígena y campesina, el maíz, los recursos naturales, la alimentación, las plantas medicinales, los cuales se analizan en diferentes contextos geográficos de México, sobre todo en comunidades de Veracruz, Chiapas, Tabasco, Estado de México y Sinaloa. La información de los textos se articula desde un enfoque general de la agricultura y el conocimiento tradicional.El presente texto está dedicado al estudio de la situación actual de los sistemas agrícolas mexicanos y su asociación con los componentes geográficos, ambientales y socioculturales, porque los recursos naturales son el sustento de los procesos y actividades agrícolas, así como del conocimiento tradicional de las familias campesinas. Por ello, se analizan las estrategias adaptativas (ambientales y socioculturales) que se vinculan con la subsistencia familiar en las comunidades rurales.Universidad Autónoma del Estado de Méxic

Transición epitelio mesénquima: de lo molecular a lo fisiológico

Resumen La transición epitelio mesénquima (EMT) es un proceso compuesto de diferentes fases, donde una célula epitelial adquiere un fenotipo mesenquimal. Dentro de los cambios involucrados se encuentran: pérdida de la polaridad celular, adquisición de una capacidad migratoria, capacidad invasora, resistencia a la apoptosis y aumento en la producción de componentes de la matriz extracelular. Todos estos cambios ocurren como una consecuencia de la activación y represión de genes involucrados con rutas de señalización específicas relacionadas con este evento. La EMT está relacionada con procesos fisiológicos y patológicos como el cáncer. Consta de tres fases: una de células no migratorias, células premigratorias y células migratorias; cada una de ellas producto de diferentes señales intra o extracelulares, factores de transcripción (TGF-B, Snail, TWIST, Sox, Slug, ZEB1, entre otras) y proteínas involucradas (E-cadherina, integrina, vimentina, ocludinas y claudinas).

The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies

International audienceSignificance There is growing evidence that preexisting autoantibodies neutralizing type I interferons (IFNs) are strong determinants of life-threatening COVID-19 pneumonia. It is important to estimate their quantitative impact on COVID-19 mortality upon SARS-CoV-2 infection, by age and sex, as both the prevalence of these autoantibodies and the risk of COVID-19 death increase with age and are higher in men. Using an unvaccinated sample of 1,261 deceased patients and 34,159 individuals from the general population, we found that autoantibodies against type I IFNs strongly increased the SARS-CoV-2 infection fatality rate at all ages, in both men and women. Autoantibodies against type I IFNs are strong and common predictors of life-threatening COVID-19. Testing for these autoantibodies should be considered in the general population