Apports du cryo-microscope électronique à balayage à émission de champ à l'étude des matières organiques et des relations organo-minérales naturelles. Application aux croûtes microbiotiques des sols Field emission cryo-scanning electron microscopy of organic matter and organomineral associations. Application to microbiotic soil crusts

The cryo-SEM (SEM equipped with a freeze-drying sample preparation system) allows the micromorphological transformations occurring during desiccation-rewetting cycles of microbiotic crusts from Sahelian soils to be visualised, like the swelling up of mucilaginous envelopes of the constituent micro-organisms through water absorption. When the cryo-SEM is equipped with a field emission gun, which permits observations at low voltage operation (1 kV) without coating, the natural surface of the samples may be revealed, in particular the presence, at the surface of mineral grains, of microbe-derived organic meshes that probably play a role in the resistance of these crusts to erosion. Résumé Le cryo-Meb (Meb équipé d'un système de préparation des échantillons par congélation-lyophilisation) permet de visualiser les modifications micromorphologiques se produisant lors des cycles de dessiccation-réhydratation des croûtes microbiotiques de sols sahéliens, comme le gonflement, par absorption d'eau, des enveloppes mucilagineuses des micro-organismes constitutifs. Lorsque le cryo-Meb possède un canon à émission de champ, qui autorise des observations à basse tension d'accélération (1 kV), sans metallisation, c'est l'état de surface naturel des échantillons qui peut être révélé, notamment la présence, autour des grains minéraux, de réseaux organiques d'origine microbienne, jouant vraisemblablement un rôle dans la résistance à l'érosion manifestée par ces croûtes

Role of a cyanobacterial cover on structural stability of sandy soils in the Sahelian part of western Niger

Microbiotic soil crusts, mostly formed by cyanobacteria, are widespread on the surface of fallow land in western Niger. They lie adjacent to completely bare soils. We have investigated the structural stability of these soils by testing aggregate breakdown under fast-wetting, slow-wetting and mechanical breakdown. The tests were effected on aggregates ranging from 3 to 5 mm in size. These experiments were completed by micromorphological examinations under light and scanning electron microscope. For all tests, the fragment size distribution and the mean weight diameter (MWD) revealed the great stability of aggregates from soils with a cyanobacterial cover (MWD 1.82 to 3.10 mm), compared to those from soils devoid of cyanobacterial cover (MWD 0.25 to 1.26 mm). Fast- and slow-wetting of microbiotic soil crust material induced a lesser disaggregation of aggregates compared to mechanical breakdown. On the contrary, fast-wetting and mechanical breakdown of aggregates from soils devoid of cyanobacterial cover induced a greater disaggregation than slow-wetting. Microscopic examination of microbiotic crusts revealed an intricate network of filamentous cyanobacteria and extracellular polymer secretions, which binds and entraps mineral particles on the soil surface. Organo-mineral aggregates ranging from 0.10 to 0.12 mm were observed. Below the superficial crusts, filaments and residual organic matter bind tightly soil particles, thus giving a compact structure. The great stability of aggregates of soil with cyanobacterial cover is likely related to the binding and gluing effect of cyanobacteria and derived organic matter. This is consistent with the positive correlation between MWD values and organic carbon content. The present results thereby confirm the resistance to erosion of soil with microbial cover as indicated by field measurements

Microbiotic soil crusts in the Sahel of Western Niger and their influence on Soil POROSITY and water dynamics

International audienceMicrobiotic soil crusts are common features of the surface of fallow land in Western Niger. We investigated the interaction between these microbial covers and the porosity and water dynamics of soils at the surface of a Sahelian landscape. The soil pore system was examined by microscopic observations and mercury porosimetry. The soil water retention capacity was measured using a Richard pressure membrane apparatus. Runoff measurements were performed in situ at a one m2 scale under natural rainfall. Samples with dense cyanobacterial cover had microbially-originated pores ranging from 0.04 and 50 μm in size. These samples also showed higher total soil porosity and retained two to four times more water than samples with thin microbial cover. These properties are closely related to the hydrophobic nature of cyanobacterial components. Final runoff values obtained on densely covered surfaces were significantly higher compared to those measured on surfaces with thin microbial cover due to the geometry of the microbially-originated pore system and its functioning

Gully erosion prediction in a Sahelian context

In Sahelian region, concentrated overland flow often leads to the formation of gullies. Although this phenomenon is widespread in those regions, research efforts are still needed to be able to model their spatial distribution and the role of the different parameters involved in this process. In this context, the objectives of this study are twofold. The first step is to investigate to what extent the role of Sahelian soil surface crusts (biological and/or physical) on soil surface infiltrability and detachment affect the formation and development of gullies. The second step is to integrate the results of these investigations in a simple geomorphological model to predict gully location at the watershed scale. The evaluation of the resulting model on two test catchments demonstrated that the integration of soil crusting is a key parameter to insure the quality and relevance of gully prediction. The model is able to distinguish between two types of gullies, those whose width range between 0.5m and 4m and those whose width exceeds 4m. The application of the model at the regional scale is however limited by the resolution of available regional digital elevation model (i.e. the 90m resolution SRTM DEM) which only permits the prediction of large gullies

Long-term effect of forest and landscape restoration practices on soil organic carbon stock in semi-arid Burkina Faso

In semi-arid areas, forest and landscape restoration (FLR) practices are being implemented to reverse the land degradation process. The objective of this study was to investigate the long-term effect of FLR practices on soil organic carbon stock (SOCs) under different land uses in the semi-arid region of Burkina Faso. The study was conducted on degraded land under rehabilitation practices for 45, 27, 18 and 11 years, which were compared to similar land without specific rehabilitation measures. The soil was collected in 2018 in 35 sampling plots of 30 m x 30 m. Soil analysis concerned bulk density, soil particle size, soil pH, soil organic carbon content, and respiratory activity of microorganisms. SOCs increased by 150%, 98% and 29% over 0-10 cm depth in 45-, 27- and 11-year of FLR practices, and decreased by 6% in 18-year of FLR practices compared to their respective control. SOCs were not linearly increased with the duration of the implementation of FLR practices because the variation of SOCs depends on several other parameters such as soil texture, and types of combination of FLR practices. The highest SOCs were recorded for 27 years (9.5 t.ha-1) and 45 years (8.5 t.ha-1) of FLR practices. This study revealed the importance of including Assisted Natural Regeneration (ANR) as one of the combined FLR practices, for improvement of SOCs

The pervasive and multifaceted influence of biocrusts on water in the world's drylands

The capture and use of water are critically important in drylands, which collectively constitute Earth's largest biome. Drylands will likely experience lower and more unreliable rainfall as climatic conditions change over the next century. Dryland soils support a rich community of microphytic organisms (biocrusts), which are critically important because they regulate the delivery and retention of water. Yet despite their hydrological significance, a global synthesis of their effects on hydrology is lacking. We synthesized 2,997 observations from 109 publications to explore how biocrusts affected five hydrological processes (times to ponding and runoff, early [sorptivity] and final [infiltration] stages of water flow into soil, and the rate or volume of runoff) and two hydrological outcomes (moisture storage, sediment production). We found that increasing biocrust cover reduced the time for water to pond on the surface (−40%) and commence runoff (−33%), and reduced infiltration (−34%) and sediment production (−68%). Greater biocrust cover had no significant effect on sorptivity or runoff rate/amount, but increased moisture storage (+14%). Infiltration declined most (−56%) at fine scales, and moisture storage was greatest (+36%) at large scales. Effects of biocrust type (cyanobacteria, lichen, moss, mixed), soil texture (sand, loam, clay), and climatic zone (arid, semiarid, dry subhumid) were nuanced. Our synthesis provides novel insights into the magnitude, processes, and contexts of biocrust effects in drylands. This information is critical to improve our capacity to manage dwindling dryland water supplies as Earth becomes hotter and drier.This work was conducted as part of the Powell Working Group “Completing the dryland puzzle: creating a predictive framework for biological soil crust function and response to climate change” supported by the John Wesley Powell Center for Analysis and Synthesis, funded by the US Geological Survey. J.B. and S.R. were funded by USGS Ecosystems and Land Use Change Mission Areas, by the US Department of Energy (DESC-0008168), and by the Strategic Environmental Research and Development Program (RC18-1322). J.D. is supported by grants from the Holsworth Wildlife Research Endowment & The Ecological Society of Australia, and a scholarship from China Scholarship Council (No. 201706040073). B.C. is supported by grants from the National Science Foundation (award DEB-1844531) and DePaul University. M.A.B. is supported by a grant from the National Science Foundation (award DEB-1638966). B.W. was supported by the Max Planck Society and a Paul Crutzen Nobel Laureate Fellowship. E.H.-S. was supported by CONACYT grant 251388 B. F.T.M. was supported by the European Research Council (ERC grant agreement 647038 [BIODESERT]) and Generalitat Valenciana (CIDEGENT/2018/041)

Soil erosion processes in european vineyards: a qualitative comparison of rainfall simulation measurements in Germany, Spain and France

Small portable rainfall simulators are considered a useful tool to analyze soil erosion processes in cultivated lands. European research groups in Spain (Valencia, Málaga, Lleida, Madrid and La Rioja), France (Reims) and Germany (Trier) have used different rainfall simulators (varying in drop size distribution and fall velocities, kinetic energy, plot forms and sizes, and field of application) to study soil loss, surface flow, runoff and infiltration coefficients in different experimental plots (Valencia, Montes de Málaga, Penedès, Campo Real and La Rioja in Spain, Champagne in France and Mosel-Ruwer valley in Germany). The measurements and experiments developed by these research teams give an overview of the variety of methodologies used in rainfall simulations to study the problem of soil erosion and describe the erosion features in different climatic environments, management practices and soil types. The aims of this study are: (i) to investigate where, how and why researchers from different wine-growing regions applied rainfall simulations with successful results as a tool to measure soil erosion processes; (ii) to make a qualitative comparison about the general soil erosion processes in European terroirs; (iii) to demonstrate the importance of the development of standard method for measurement of soil erosion processes in vineyards, using rainfall simulators; and (iv) and to analyze the key factors that should be taken into account to carry out rainfall simulations. The rainfall simulations in all cases allowed infiltration capacity, susceptibility of the soil to detachment and generation of sediment loads to runoff to be determined. Despite using small plots, the experiments were useful to analyze the influence of soil cover to reduce soil erosion, to make comparisons between different locations, and to evaluate the influence of different soil characteristics. The comparative analysis of the studies performed in different study areas points out the need to define an operational methodology to carry out rainfall simulations, which allows us to obtain representative and comparable results and to avoid errors in the interpretation in order to achieve comparable information about runoff and soil los

Hotspots of biogeochemical activity linked to aridity and plant traits across global drylands

14 páginas.- 4 figuras.- 67 referencias.- The online version contains supplementary material available at https://doi.org/10.1038/s41477-024-01670-7Perennial plants create productive and biodiverse hotspots, known as fertile islands, beneath their canopies. These hotspots largely determine the structure and functioning of drylands worldwide. Despite their ubiquity, the factors controlling fertile islands under conditions of contrasting grazing by livestock, the most prevalent land use in drylands, remain virtually unknown. Here we evaluated the relative importance of grazing pressure and herbivore type, climate and plant functional traits on 24 soil physical and chemical attributes that represent proxies of key ecosystem services related to decomposition, soil fertility, and soil and water conservation. To do this, we conducted a standardized global survey of 288 plots at 88 sites in 25 countries worldwide. We show that aridity and plant traits are the major factors associated with the magnitude of plant effects on fertile islands in grazed drylands worldwide. Grazing pressure had little influence on the capacity of plants to support fertile islands. Taller and wider shrubs and grasses supported stronger island effects. Stable and functional soils tended to be linked to species-rich sites with taller plants. Together, our findings dispel the notion that grazing pressure or herbivore type are linked to the formation or intensification of fertile islands in drylands. Rather, our study suggests that changes in aridity, and processes that alter island identity and therefore plant traits, will have marked effects on how perennial plants support and maintain the functioning of drylands in a more arid and grazed world.This research was supported by the European Research Council (ERC grant 647038 (BIODESERT) awarded to F.T.M.) and Generalitat Valenciana (CIDEGENT/2018/041). D.J.E. was supported by the Hermon Slade Foundation (HSF21040). J. Ding was supported by the National Natural Science Foundation of China Project (41991232) and the Fundamental Research Funds for the Central Universities of China. M.D.-B. acknowledges support from TED2021-130908B-C41/AEI/10.13039/501100011033/Unión Europea Next Generation EU/PRTR and the Spanish Ministry of Science and Innovation for the I + D + i project PID2020-115813RA-I00 funded by MCIN/AEI/10.13039/501100011033. O.S. was supported by US National Science Foundation (Grants DEB 1754106, 20-25166), and Y.L.B.-P. by a Marie Sklodowska-Curie Actions Individual Fellowship (MSCA-1018 IF) within the European Program Horizon 2020 (DRYFUN Project 656035). K.G. and N.B. acknowledge support from the German Federal Ministry of Education and Research (BMBF) SPACES projects OPTIMASS (FKZ: 01LL1302A) and ORYCS (FKZ: FKZ01LL1804A). B.B. was supported by the Taylor Family-Asia Foundation Endowed Chair in Ecology and Conservation Biology, and M. Bowker by funding from the School of Forestry, Northern Arizona University. C.B. acknowledges funding from the National Natural Science Foundation of China (41971131). D.B. acknowledges support from the Hungarian Research, Development and Innovation Office (NKFI KKP 144096), and A. Fajardo support from ANID PIA/BASAL FB 210006 and the Millennium Science Initiative Program NCN2021-050. M.F. and H.E. received funding from Ferdowsi University of Mashhad (grant 39843). A.N. and M.K. acknowledge support from FCT (CEECIND/02453/2018/CP1534/CT0001, SFRH/BD/130274/2017, PTDC/ASP-SIL/7743/2020, UIDB/00329/2020), EEA (10/CALL#5), AdaptForGrazing (PRR-C05-i03-I-000035) and LTsER Montado platform (LTER_EU_PT_001) grants. O.V. acknowledges support from the Hungarian Research, Development and Innovation Office (NKFI KKP 144096). L.W. was supported by the US National Science Foundation (EAR 1554894). Y.Z. and X.Z. were supported by the National Natural Science Foundation of China (U2003214). H.S. is supported by a María Zambrano fellowship funded by the Ministry of Universities and European Union-Next Generation plan. The use of any trade, firm or product names does not imply endorsement by any agency, institution or government. Finally, we thank the many people who assisted with field work and the landowners, corporations and national bodies that allowed us access to their land.Peer reviewe