15 research outputs found
Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products
Effect of disclosing the relation between effort and unit reliability on system reliability: An economic experiment
Open Flower Fluoroimmunoassay: A General Method To Make Fluorescent Protein-Based Immunosensor Probes
Fluorescence-based probes, especially
those that utilize Förster
resonance energy transfer (FRET) between fluorescent protein (FP)
variants, are widely used to monitor various biological phenomena,
most often detecting its ligand-induced conformational change through
the receptor domain. While antibody provides a fertile resource of
a specific receptor for various biomolecules, its potential has not
been fully exploited. An exception is a pair of donor FP-fused V<sub>H</sub> and acceptor FP-fused V<sub>L</sub> fragments, which has
been proven useful when their association increases in the presence
of antigen (open sandwich fluoroimmunoassay, OS-FIA). However, probes
for larger proteins such as serum albumin (SA) were difficult to produce,
since the interaction between V<sub>H</sub> and V<sub>L</sub> of these
antibodies is barely affected by the bound antigen. Here, we propose
a novel strategy, called open flower fluoroimmunoassay (OF-FIA), using
a probe composed of a donor-fused V<sub>H</sub> and an acceptor-fused
V<sub>L</sub> linked by a disulfide bond between V<sub>H</sub> and
V<sub>L</sub> (CyPet/YPet-dsFv). The probe gave high FRET efficiency
due to the dimerization propensity of the FP pair, while the efficiency
got lower as SA concentration increased, probably due to dimer disruption.
The constructed probe could detect clinically relevant range of SA,
showing its potential as a diagnostic reagent
Erratum to “DNA amplification using phi29 DNA polymerase validates gene polymorphism analysis from buccal mucosa samples” [J. Prosthodont. Res. 55 (2011) 165–170]
Synthesis of Network Biobased Aliphatic Polyesters Exhibiting Better Tensile Properties than the Linear Polymers by ADMET Polymerization in the Presence of Glycerol Tris(undec-10-enoate)
Development of biobased aliphatic polyesters with better mechanical (tensile) properties in film has attracted considerable attention. This report presents the synthesis of soluble network biobased aliphatic polyesters by acyclic diene metathesis (ADMET) polymerization of bis(undec-10-enyl)isosorbide diester [M1, dianhydro-D-glucityl bis(undec-10-enoate)] in the presence of a tri-arm crosslinker [CL, glycerol tris(undec-10-enoate)] using a ruthenium–carbene catalyst, and subsequent olefin hydrogenation using RhCl(PPh3)3. The resultant polymers, after hydrogenation (expressed as HCP1) and prepared in the presence of 1.0 mol% CL, showed better tensile properties than the linear polymer (HP1) with similar molecular weight [tensile strength (elongation at break): 20.8 MPa (282%) in HP1 vs. 35.4 MPa (572%) in HCP1]. It turned out that the polymer films prepared by the addition of CL during the polymerization (expressed as a 2-step approach) showed better tensile properties. The resultant polymer film also shows better tensile properties than the conventional polyolefins such as linear high density polyethylene, polypropylene, and low density polyethylene