Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

D-Aspartate (D-Asp) treatment improved the fertility of young male C57BL/6N mice in vivo revealing a direct role on capacitation, acrosome reaction, and fertility in vitro in young males only. We investigated whether the positive effect of D-Asp on fertility could be extended to adult males and evaluated the efficacy of a 2- or 4-week-treatment in vivo. Therefore, 20 mM sodium D-Asp was supplied in drinking water to males of different ages so that they were 9 or 16 weeks old at the end of the experiments. After sperm freezing, the in vitro fertilization (IVF) rate, the birth rate, hormone levels (luteinizing hormone (LH), epitestosterone, and testosterone), the sperm quality (morphology, abnormalities, motility, and velocity), the capacitation rate, and the acrosome reaction were investigated. Oral D-Asp treatment improves the fertilizing capability in mice regardless of the age of the animals. Importantly, a short D-Asp treatment of 2 weeks in young males elevates sperm parameters to the levels of untreated adult animals. In vivo, D-Asp treatment highly improves sperm quality but not sperm concentration. Therefore, D-Asp plays a beneficial role in mouse male fertility and may be highly relevant for cryorepositories to improve mouse sperm biobanking

Geochemical Analysis of Recent Volcanic Ash Blanketing Barbados and Constraints on Magma Composition (V32E-0112)

Preliminary field and geochemical investigations were conducted on ash samples from Barbados to chemically characterize and decipher magma chemistry associated with a recent volcanic event. A thick plume of volcanic ash from the La Soufriere Volcano in St Vincent, which erupted on April 9, 2021, caused lower visibility due to ash clouds which engulfed the region for some time. Three authors from the research team visited Barbados during the summer subsequent and completed a reconnaissance investigation on recent ashfall. St Vincent is a small volcanic island in the Eastern Caribbean and lies in the southern part of the Lesser Antilles volcanic arc. Six volcanoes along the arc have erupted since 1900. Magmas that have erupted from the Soufrière of St Vincent during the geologic past (Holocene) include primitive arc basalts and andesites. Collected ash samples were analyzed using inductively coupled plasma mass spectrometry (ICP-MS), Ultra Trace Aqua Regia and whole rock package – X-ray Fluorescence (XRF). Facilities available through the ALS USA Incorporated (Reno, Nevada), an established geochemistry laboratory known for its research quality output, provided access to retrieve both bulk oxide and trace elements data. To achieve better accuracy, samples were pulverized to 75 microns or less. Analyzed samples fell well within the more evolved andesitic compositions (SiO2 = 53- 56 wt. %). A notable concentration of alumina (18%), CaO (9%), and a general decrease in magnesia and soda (each averaging 3%) seem to suggest differentiation of the investigated samples. LOI recorded between less than 1 to 3%. It seems likely that magma evolution was substantially driven by crystal fractionation over time. The results of trace element investigation including Ba, Cu, Mn, Ce, Cr, Ni, Zn, Rb, Sr, V, Y, and Zr, point to an intermediate volcanism constraining the ash composition in Barbados. Utilization of Harker-type binary variation diagrams involving recent ash deposits in Barbados indicate differentiation trend with an evolving nature of the source volcanism. Composition of ash samples record time-integrated enrichment in light REE over heavy REE, and the volcanism in the source area changed character with time and point to an increase in the degree of partial melting and a decrease in crustal involvement with time

Comparison of clastogen-induced gene expression profiles in wild-type and DNA repair-deficient Rad54/Rad54B cells

Background: Previously we found that Rad54/Rad54B cells are more sensitive towards mitomycin C (MMC) as compared to wild-type (WT) cells. This difference in sensitivity was absent upon exposure to other clastogens like bleomycin (BLM) and g-radiation. In order to get further insight into possible underlying mechanisms, gene expression changes in WT and Rad54/Rad54B MEFs (mouse embryonic fibroblasts) after exposure to the clastogens MMC and BLM were investigated. Exposures of these cells to mutagens (N-ac-AAF and ENU) and vehicle were taken as controls. Results: Most exposures resulted in an induction of DNA damage signaling and apoptosis genes and a reduced expression of cell division genes in cells of both genotypes. As expected, responses to N-ac-AAF were very similar in both genotypes. ENU exposure did not lead to significant gene expression changes in cells of both genotypes, presumably due to its short half-life. Gene expression responses to clastogens, however, showed a genotype-dependent effect for BLM and MMC. MMC treated Rad54/Rad54B MEFs showed no induction of p53-signaling, DNA damage response and apoptosis as seen for all the other treatments. Conclusion: These data support our finding that different types of clastogens exist and that responses to these types depend on the DNA repair status of the cells.Toxicogenomics and risk assessmen

Neoflavonoids as Inhibitors of HIV-1 Replication by Targeting the Tat and NF-kB Pathways

Twenty-eight neoflavonoids have been prepared and evaluated in vitro against HIV-1. Antiviral activity was assessed on MT-2 cells infected with viral clones carrying the luciferase reporter gene. Inhibition of HIV transcription and Tat function were tested on cells stably transfected with the HIV-LTR and Tat protein. Seven 4-phenylchromen-2-one derivatives showed HIV transcriptional inhibitory activity but only the phenylchrome-2-one 10 inhibited NF- B and displayed anti-Tat activity simultaneously. Compounds 10, 14, and 25, inhibited HIV replication in both targets at concentrations <25 M. The assays of these synthetic 4-phenylchromen-2-ones may aid in the investigation of some aspects of the anti-HIV activity of such compounds and could serve as a scaffold for designing better anti-HIV compounds, which may lead to a potential anti-HIV therapeutic dru

NASA MAA (MUREP AEROSPACE ACADEMY) STEM PROJECT AT YORK COLLEGE: ENSURING FUTURE STEM PIPELINE

Our ongoing K1-12 NASA-supported STEM activities has served over 5000 children since 2015, largely from groups that are undeserved and underrepresented in the sciences. These STEM activities have been successfully delivered for several years and include earth science, meteorite geology, rocketry, aerodynamics, star gazing, EV3 Mindstorm robotics, basic coding exercises, wind tunnel, 3D-printing, etc., conducted largely through our present MAA (MUREP Aerospace Academy) Project. Corporate funding from the Con Edison and National Grid ensures additional STEM dissemination to the students during the summer operation. Significant positive component of the MAA program is the availability and willingness of the former student participants (many of whom are completing STEM majors in college) to extend their helping hands to assist teachers and guide students to fulfill assigned tasks and share their rich experience to reinforce the value of STEM learning. In a way, they validate students’ participation and promote meaningful dialog with their parents, mostly first generation Americans, often with English as a second language, and lacking knowledge of the importance of the STEM disciplines and careers built around it. Recent involvement of the City University of New York’s (CUNY) Explorer’s Program, a vigorous initiative undertaken by the CUNY to bring thousands of middle school students to the various CUNY campuses, including York College, opened a formidable partnership building opportunity for the MAA to tap into the future pipeline by engaging visiting students with our STEM facilities and educating these young and curious minds about the future STEM potential both in selecting exciting academic and career options. STEM outreach delivered through hands-on, team work, in an experiential and critical thinking environment is becoming a catalyst for motivating numerous students towards earth science and relevant NASA content. This way, MAA Program is creating a STEM conscious young student body and providing a strategic recruitment tool for various undergraduate STEM disciplines. Early involvement of middle school students in STEM activities can be deemed as a powerful and viable mechanism to overcome an apparent shortage of STEM workforce representing minorities, women and financially disadvantaged groups

Caracterización fitoquimica de extractos de frondas y rizomas de dos especies del genero Phlebodium provenientes de Honduras y Guatemala

El complejo calahuala son especies de la familia Polypodiaceae, (Phlebodium pseudoaureum y P. decamanum) utilizadas comúnmente para el tratamiento de afecciones gastrointestinales, respiratorias, cardíacas, renales, reumatismo, diabetes, hipertensión, tópicamente se usa en psoriasis, eczema, úlceras y como protector solar. Se ha demostrado por pruebas farmacológicas actividad antiinflamatoria, inmunomoduladora, fotoprotectiva, y estimuladora de la actividad cerebral. En el presente trabajo se comparó el perfil cromatográfico de extractos de frondas y rizomas de P. decamanum y P pseudoaureum mediante cromatografía en capa fina (TLC) y cromatografía líquida de alta resolución (HPLC) con el objetivo de determinar diferencias fitoquímicas entre las especies y proponer algunas moléculas que pudieran usarse para estandarizar sus extractos

Non-homologous end-joining pathway associated with occurrence of myocardial infarction: gene set analysis of genome-wide association study data

&lt;p&gt;Purpose: DNA repair deficiencies have been postulated to play a role in the development and progression of cardiovascular disease (CVD). The hypothesis is that DNA damage accumulating with age may induce cell death, which promotes formation of unstable plaques. Defects in DNA repair mechanisms may therefore increase the risk of CVD events. We examined whether the joints effect of common genetic variants in 5 DNA repair pathways may influence the risk of CVD events.&lt;/p&gt; &lt;p&gt;Methods: The PLINK set-based test was used to examine the association to myocardial infarction (MI) of the DNA repair pathway in GWAS data of 866 subjects of the GENetic DEterminants of Restenosis (GENDER) study and 5,244 subjects of the PROspective Study of Pravastatin in the Elderly at Risk (PROSPER) study. We included the main DNA repair pathways (base excision repair, nucleotide excision repair, mismatch repair, homologous recombination and non-homologous end-joining (NHEJ)) in the analysis.&lt;/p&gt; &lt;p&gt;Results: The NHEJ pathway was associated with the occurrence of MI in both GENDER (P = 0.0083) and PROSPER (P = 0.014). This association was mainly driven by genetic variation in the MRE11A gene (PGENDER = 0.0001 and PPROSPER = 0.002). The homologous recombination pathway was associated with MI in GENDER only (P = 0.011), for the other pathways no associations were observed.&lt;/p&gt; &lt;p&gt;Conclusion: This is the first study analyzing the joint effect of common genetic variation in DNA repair pathways and the risk of CVD events, demonstrating an association between the NHEJ pathway and MI in 2 different cohorts.&lt;/p&gt