10 research outputs found
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Photonic Crystal Kinase Biosensor
We have developed
a novel biosensor for kinases that is based on
a kinase-responsive polymer hydrogel, which enables label-free screening
of kinase activity via changes in optical properties. The hydrogel
is specifically designed to swell reversibly upon phosphorylation
of a target peptide, triggering a change in optical diffraction from
a crystalline colloidal array of particles impregnated into the hydrogel.
Diffraction measurements, and charge staining, confirmed the responsive
nature of the hydrogel. Moreover, the change in diffraction of the
hydrogel upon treatment with kinase exhibited a time- and dose-dependent
response. A theoretical model for ionic polymer networks describes
the observed optical response well and can be used to quantify the
extent of phosphorylation