10 research outputs found
Recommended from our members
Notes vs Recall: Can Third-Year Medical Students Benefit from Obtaining and Presenting an HPI Without Using Notes?
Get PDFRecommended from our members
The Patient Experience Curriculum: Increasing Medical Student Awareness of Patient Centered Care
No full textRecommended from our members
The Patient Experience Curriculum: Increasing Medical Student Awareness of Patient Centered Care
No full textPhotonic Crystal Kinase Biosensor
No full textWe have developed
a novel biosensor for kinases that is based on
a kinase-responsive polymer hydrogel, which enables label-free screening
of kinase activity via changes in optical properties. The hydrogel
is specifically designed to swell reversibly upon phosphorylation
of a target peptide, triggering a change in optical diffraction from
a crystalline colloidal array of particles impregnated into the hydrogel.
Diffraction measurements, and charge staining, confirmed the responsive
nature of the hydrogel. Moreover, the change in diffraction of the
hydrogel upon treatment with kinase exhibited a time- and dose-dependent
response. A theoretical model for ionic polymer networks describes
the observed optical response well and can be used to quantify the
extent of phosphorylation
Label-free detection of missense mutations and methylation differences in the p53 gene using optically diffracting hydrogels
No full text
