Revisiting non-offspring nursing: Allonursing evolves when the costs are low

This is the accepted manuscript. The final published version is available from Royal Society Publishing at http://rsbl.royalsocietypublishing.org/content/10/6/20140378.abstract.Allonursing, the nursing of another female's offspring, is commonly assumed to have evolved through the benefits of kin selection or reciprocity. The evolution of allonursing may also be influenced by variation in the possible costs to allonurses. The relative influence of costs and benefits on the incidence of allonursing in mammals remains unexplored. We show, using comparative analyses, that where females group with kin, the presence or the absence of allonursing is not associated with further variation in relatedness. Allonursing is most common where females produce litters; here the relative investment per offspring is low, and the costs of nursing additional young are likely to be reduced. Our results suggest that variation in the potential benefits is not associated with the distribution of allonursing, but that allonursing can quickly evolve when the costs to allonurses of nursing additional offspring are low.D.L. received funding from ERC grant no. 294494-THCB2011

Minutes matter: brief hatching asynchrony adversely affects late-hatched hihi nestlings, but not life beyond the nest

Size hierarchies are often seen when nestlings hatch asynchronously over a period of days. Shorter hatch periods are common across passerines, however, and while these may also give rise to asymmetries, their effects are rarely considered. Regardless of hatch period, the long-term consequences for later hatched nestlings that survive to fledge is unknown for wild birds. Here we explored the timing of hatch order in a free-living population of hihi nestlings, Notiomystis cincta, and followed any effects in and out of the nest. We found that while hatching time from first- to last-hatched nestlings was often less than 24 h, last-hatched individuals grew more slowly and were lighter and smaller at fledging than older siblings. Last-hatched nestlings were also less likely to fledge. These effects were greater in larger broods. Adult body size is correlated with fledging size in hihi; however, we found no evidence that hatch order affected longevity postfledging, or lifetime reproductive success. We then explored whether carotenoid availability might buffer these stressful rearing conditions (through food supplementation of parents) but found no evidence that increased access to carotenoids for mothers and/or growing nestlings influenced incubation schedules, or the effects of hatching late. Together these results suggest that while even a very short hatch period can influence adult phenotype, hatching asynchrony is not maladaptive for hihi: when last-hatched nestlings survive to fledge they can contribute as much to their mothers' fitness as first-hatched siblings

Minutes matter: brief hatching asynchrony adversely affects late-hatched hihi nestlings, but not life beyond the nest

Size hierarchies are often seen when nestlings hatch asynchronously over a period of days. Shorter hatch periods are common across passerines, however, and while these may also give rise to asymmetries, their effects are rarely considered. Regardless of hatch period, the long-term consequences for later hatched nestlings that survive to fledge is unknown for wild birds. Here we explored the timing of hatch order in a free-living population of hihi nestlings, Notiomystis cincta, and followed any effects in and out of the nest. We found that while hatching time from first- to last-hatched nestlings was often less than 24 h, last-hatched individuals grew more slowly and were lighter and smaller at fledging than older siblings. Last-hatched nestlings were also less likely to fledge. These effects were greater in larger broods. Adult body size is correlated with fledging size in hihi; however, we found no evidence that hatch order affected longevity postfledging, or lifetime reproductive success. We then explored whether carotenoid availability might buffer these stressful rearing conditions (through food supplementation of parents) but found no evidence that increased access to carotenoids for mothers and/or growing nestlings influenced incubation schedules, or the effects of hatching late. Together these results suggest that while even a very short hatch period can influence adult phenotype, hatching asynchrony is not maladaptive for hihi: when last-hatched nestlings survive to fledge they can contribute as much to their mothers' fitness as first-hatched siblings

Survey of the quality of experimental design, statistical analysis and reporting of research using animals

For scientific, ethical and economic reasons, experiments involving animals should be appropriately designed, correctly analysed and transparently reported. This increases the scientific validity of the results, and maximises the knowledge gained from each experiment. A minimum amount of relevant information must be included in scientific publications to ensure that the methods and results of a study can be reviewed, analysed and repeated. Omitting essential information can raise scientific and ethical concerns. We report the findings of a systematic survey of reporting, experimental design and statistical analysis in published biomedical research using laboratory animals. Medline and EMBASE were searched for studies reporting research on live rats, mice and non-human primates carried out in UK and US publicly funded research establishments. Detailed information was collected from 271 publications, about the objective or hypothesis of the study, the number, sex, age and/or weight of animals used, and experimental and statistical methods. Only 59% of the studies stated the hypothesis or objective of the study and the number and characteristics of the animals used. Appropriate and efficient experimental design is a critical component of high-quality science. Most of the papers surveyed did not use randomisation (87%) or blinding (86%), to reduce bias in animal selection and outcome assessment. Only 70% of the publications that used statistical methods described their methods and presented the results with a measure of error or variability. This survey has identified a number of issues that need to be addressed in order to improve experimental design and reporting in publications describing research using animals. Scientific publication is a powerful and important source of information; the authors of scientific publications therefore have a responsibility to describe their methods and results comprehensively, accurately and transparently, and peer reviewers and journal editors share the responsibility to ensure that published studies fulfil these criteria

Chromatic Illumination Discrimination Ability Reveals that Human Colour Constancy Is Optimised for Blue Daylight Illuminations

The phenomenon of colour constancy in human visual perception keeps surface colours constant, despite changes in their reflected light due to changing illumination. Although colour constancy has evolved under a constrained subset of illuminations, it is unknown whether its underlying mechanisms, thought to involve multiple components from retina to cortex, are optimised for particular environmental variations. Here we demonstrate a new method for investigating colour constancy using illumination matching in real scenes which, unlike previous methods using surface matching and simulated scenes, allows testing of multiple, real illuminations. We use real scenes consisting of solid familiar or unfamiliar objects against uniform or variegated backgrounds and compare discrimination performance for typical illuminations from the daylight chromaticity locus (approximately blue-yellow) and atypical spectra from an orthogonal locus (approximately red-green, at correlated colour temperature 6700 K), all produced in real time by a 10-channel LED illuminator. We find that discrimination of illumination changes is poorer along the daylight locus than the atypical locus, and is poorest particularly for bluer illumination changes, demonstrating conversely that surface colour constancy is best for blue daylight illuminations. Illumination discrimination is also enhanced, and therefore colour constancy diminished, for uniform backgrounds, irrespective of the object type. These results are not explained by statistical properties of the scene signal changes at the retinal level. We conclude that high-level mechanisms of colour constancy are biased for the blue daylight illuminations and variegated backgrounds to which the human visual system has typically been exposed

Lattice Boltzmann method for the fractional advection-diffusion equation

Mass transport, such as movement of phosphorus in soils and solutes in rivers, is a natural phenomenon and its study plays an important role in science and engineering. It is found that there are numerous practical diffusion phenomena that do not obey the classical advection-diffusion equation (ADE). Such diffusion is called abnormal or superdiffusion, and it is well described using a fractional advection-diffusion equation (FADE). The FADE finds a wide range of applications in various areas with great potential for studying complex mass transport in real hydrological systems. However, solution to the FADE is difficult, and the existing numerical methods are complicated and inefficient. In this study, a fresh lattice Boltzmann method is developed for solving the fractional advection-diffusion equation (LabFADE). The FADE is transformed into an equation similar to an advection-diffusion equation and solved using the lattice Boltzmann method. The LabFADE has all the advantages of the conventional lattice Boltzmann method and avoids a complex solution procedure, unlike other existing numerical methods. The method has been validated through simulations of several benchmark tests: a point-source diffusion, a boundary-value problem of steady diffusion, and an initial-boundary-value problem of unsteady diffusion with the coexistence of source and sink terms. In addition, by including the effects of the skewness β , the fractional order α , and the single relaxation time τ , the accuracy and convergence of the method have been assessed. The numerical predictions are compared with the analytical solutions, and they indicate that the method is second-order accurate. The method presented will allow the FADE to be more widely applied to complex mass transport problems in science and engineering

Facial expressions depicting compassionate and critical emotions: the development and validation of a new emotional face stimulus set

Attachment with altruistic others requires the ability to appropriately process affiliative and kind facial cues. Yet there is no stimulus set available to investigate such processes. Here, we developed a stimulus set depicting compassionate and critical facial expressions, and validated its effectiveness using well-established visual-probe methodology. In Study 1, 62 participants rated photographs of actors displaying compassionate/kind and critical faces on strength of emotion type. This produced a new stimulus set based on N = 31 actors, whose facial expressions were reliably distinguished as compassionate, critical and neutral. In Study 2, 70 participants completed a visual-probe task measuring attentional orientation to critical and compassionate/kind faces. This revealed that participants lower in self-criticism demonstrated enhanced attention to compassionate/kind faces whereas those higher in self-criticism showed no bias. To sum, the new stimulus set produced interpretable findings using visual-probe methodology and is the first to include higher order, complex positive affect displays

Differences between <i>Trypanosoma brucei gambiense</i> groups 1 and 2 in their resistance to killing by Trypanolytic factor 1

&lt;p&gt;&lt;b&gt;Background:&lt;/b&gt; The three sub-species of &lt;i&gt;Trypanosoma brucei&lt;/i&gt; are important pathogens of sub-Saharan Africa. &lt;i&gt;T. b. brucei&lt;/i&gt; is unable to infect humans due to sensitivity to trypanosome lytic factors (TLF) 1 and 2 found in human serum. &lt;i&gt;T. b. rhodesiense&lt;/i&gt; and &lt;i&gt;T. b. gambiense&lt;/i&gt; are able to resist lysis by TLF. There are two distinct sub-groups of &lt;i&gt;T. b. gambiense&lt;/i&gt; that differ genetically and by human serum resistance phenotypes. Group 1 &lt;i&gt;T. b. gambiense&lt;/i&gt; have an invariant phenotype whereas group 2 show variable resistance. Previous data indicated that group 1 &lt;i&gt;T. b. gambiense&lt;/i&gt; are resistant to TLF-1 due in-part to reduced uptake of TLF-1 mediated by reduced expression of the TLF-1 receptor (the haptoglobin-hemoglobin receptor (&lt;i&gt;HpHbR&lt;/i&gt;)) gene. Here we investigate if this is also true in group 2 parasites.&lt;/p&gt; &lt;p&gt;&lt;b&gt;Methodology:&lt;/b&gt; Isogenic resistant and sensitive group 2 &lt;i&gt;T. b. gambiense&lt;/i&gt; were derived and compared to other T. brucei parasites. Both resistant and sensitive lines express the &lt;i&gt;HpHbR&lt;/i&gt; gene at similar levels and internalized fluorescently labeled TLF-1 similar fashion to &lt;i&gt;T. b. brucei&lt;/i&gt;. Both resistant and sensitive group 2, as well as group 1 &lt;i&gt;T. b. gambiense&lt;/i&gt;, internalize recombinant APOL1, but only sensitive group 2 parasites are lysed.&lt;/p&gt; &lt;p&gt;&lt;b&gt;Conclusions:&lt;/b&gt; Our data indicate that, despite group 1 &lt;i&gt;T. b. gambiense&lt;/i&gt; avoiding TLF-1, it is resistant to the main lytic component, APOL1. Similarly group 2 &lt;i&gt;T. b. gambiense&lt;/i&gt; is innately resistant to APOL1, which could be based on the same mechanism. However, group 2 &lt;i&gt;T. b. gambiense&lt;/i&gt; variably displays this phenotype and expression does not appear to correlate with a change in expression site or expression of &lt;i&gt;HpHbR&lt;/i&gt;. Thus there are differences in the mechanism of human serum resistance between &lt;i&gt;T. b. gambiense&lt;/i&gt; groups 1 and 2.&lt;/p&gt