82 research outputs found
Citrullinated histone H3 as a novel prognostic blood marker in patients with advanced cancer
- Author
- A Jemal
- AM Houghton
- Annika Lundström
- B Korkmaz
- B Korkmaz
- BA Boone
- C Alfaro
- C Anzilotti
- C Thalin
- C Thalin
- C Yang
- Cedric Seignez
- Charlotte ThÄlin
- E Podaza
- F Semeraro
- H Huang
- HS Jethwa
- HÄkan Wallén
- I Neeli
- J Cedervall
- J Cools-Lartigue
- J Hazeldine
- J Park
- J Xu
- JA Foekens
- K Helin
- K Kawabata
- K Kessenbrock
- L Eriksson
- L Visconti
- M Demers
- M Demers
- M Demers
- M Leshner
- M Saffarzadeh
- M Stroun
- Maud Daleskog
- MB Joshi
- ME Charlson
- MF Sanmamed
- Mia Phillipson
- ML Ekaney
- MĂ©lanie Demers
- Nades Palaniyar
- P Li
- PB Gahan
- Peter Henriksson
- R Allam
- RS Keshari
- RW Hornung
- S Arelaki
- S Guglietta
- S Holdenrieder
- S Najmeh
- S Tohme
- S Wang
- SJ Klebanoff
- SK Jorch
- ST Abrams
- Staffan Lundström
- TA Fuchs
- TA Fuchs
- TA Fuchs
- Thomas Helleday
- TL Wu
- V Brinkmann
- WW Lin
- X Chang
- Y Wang
- ZH Huang
- ZT Schafer
- Publication venue
- 'Public Library of Science (PLoS)'
- Publication date
- 01/01/2018
- Field of study
Citrullinated histone H3 (H3Cit) is a central player in the neutrophil release of nuclear chromatin, known as neutrophil extracellular traps (NETs). NETs have been shown to elicit harmful effects on the host, and were recently proposed to promote tumor progression and spread. Here we report significant elevations of plasma H3Cit in patients with advanced cancer compared with age-matched healthy individuals. These elevations were specific to cancer patients as no increase was observed in severely ill and hospitalized patients with a higher non-malignant comorbidity. The analysis of neutrophils from cancer patients showed a higher proportion of neutrophils positive for intracellular H3Cit compared to severely ill patients. Moreover, the presence of plasma H3Cit in cancer patients strongly correlated with neutrophil activation markers neutrophil elastase (NE) and myeloperoxidase (MPO), and the inflammatory cytokines interleukin-6 and -8, known to induce NETosis. In addition, we show that high levels of circulating H3Cit strongly predicted poor clinical outcome in our cohort of cancer patients with a 2-fold increased risk for short-term mortality. Our results also corroborate the association of NE, interleukin-6 and -8 with poor clinical outcome. Taken together, our results are the first to unveil H3Cit as a potential diagnostic and prognostic blood marker associated with an exacerbated inflammatory response in patients with advanced cancer
Expression analysis onto microarrays of randomly selected cDNA clones highlights HOXB13 as a marker of human prostate cancer
- Author
- A Ben Dor
- A Paoloni-Giacobino
- B Lin
- C Jung
- C Kumar-Sinha
- CA Podlasek
- CA Podlasek
- CJ Bieberich
- D Price
- D Singh
- DG Rees
- DR Rhodes
- E LaTulippe
- EP Gelmann
- J Clark
- J Luo
- J Xu
- JA Magee
- JB Welsh
- JH Luo
- KD Economides
- KS Korkmaz
- LL Xu
- M Wilkinson
- MF Darson
- MJ Burger
- P Berthon
- P Olsson
- PJ Sciavolino
- R Bhatia-Gaur
- R Kuefer
- R Manda
- S Varambally
- SA Abdulkadir
- SM Dhanasekaran
- T Ernst
- T Kondo
- T Sreenath
- TA Stamey
- TA Stamey
- V Srikantan
- X Warot
- XF Liu
- XJ Ma
- Publication venue
- Nature Publishing Group
- Publication date
- 07/12/2004
- Field of study
In a strategy aimed at identifying novel markers of human prostate cancer, we performed expression analysis using microarrays of clones randomly selected from a cDNA library prepared from the LNCaP prostate cancer cell line. Comparisons of expression profiles in primary human prostate cancer, adjacent normal prostate tissue, and a selection of other (nonprostate) normal human tissues, led to the identification of a set of clones that were judged as the best candidate markers of normal and/or malignant prostate tissue. DNA sequencing of the selected clones revealed that they included 10 genes that had previously been established as prostate markers: NKX3.1, KLK2, KLK3 (PSA), FOLH1 (PSMA), STEAP2, PSGR, PRAC, RDH11, Prostein and FASN. Following analysis of the expression patterns of all selected and sequenced genes through interrogation of SAGE databases, a further three genes from our clone set, HOXB13, SPON2 and NCAM2, emerged as additional candidate markers of human prostate cancer. Quantitative RTâPCR demonstrated the specificity of expression of HOXB13 in prostate tissue and revealed its ubiquitous expression in a series of 37 primary prostate cancers and 20 normal prostates. These results demonstrate the utility of this expression-microarray approach in hunting for new markers of individual human cancer types
The use of Fluorescence Resonance Energy Transfer (FRET) peptidesfor measurement of clinically important proteolytic enzymes
- Author
- Adriana K. Carmona
- ALVES FM
- ALVES MF
- ALVES MF
- ANGELO PF
- ARAUJO MC
- BARROS NM
- BERSANETTI PA
- CAMPOS M
- CARMEL A
- CARMONA AK
- CHAGAS JR
- CHAGAS JR
- COTRIN SS
- CUSHMAN DW
- DE SOUZA ES
- DEL NERY E
- DEL NERY E
- DION N
- EHLERS MR
- FLORENTIN D
- FOSTER T
- FRIEDLAND J
- GOUDREAU N
- GROISMAN GM
- HERSH LB
- HIRATA IY
- IWATA N
- JASPARD E
- JOHANNING K
- KERR MA
- KORKMAZ B
- LETARTE M
- Luiz Juliano
- LĂPEZ-OTĂN C
- MALFROY B
- MALFROY B
- MALFROY B
- Maria Aparecida Juliano
- MEDEIROS MA
- MOLINARO G
- MOLINARO G
- OLIVEIRA MC
- PIMENTA DC
- PORTARO FC
- PORTARO FC
- PRYDE
- PUZER L
- PUZER L
- RONCO P
- ROQUES BP
- ROUSSEAU A
- RĂHAULT S
- SABATINI RA
- SAPSFORD KE
- SCHECHTER I
- SKEGGS LT
- SKIDGEL RA
- SKIDGEL RA
- SOUBRIER F
- TURK B
- VASILJEVA O
- WEI L
- WEI L
- WELSCH C
- WHITWORTH JA
- YANG HYT
- Publication venue
- 'FapUNIFESP (SciELO)'
- Publication date
- Field of study
Global patient outcomes after elective surgery: prospective cohort study in 27 low-, middle- and high-income countries.
- Author
- Ab Majid MA
- Ab Rahman AS
- Ab Rahman R
- Abayasinghe C
- Abbott T
- Abdullah NH
- Abdur-Rahman L
- Abeloos J
- Abernethy C
- Abidin UN
- Abrunhosa A
- Absar M
- Adam S
- Adams D
- Adams G
- Adamson M
- Adekola O
- Adelaja Y
- Ademola A
- Adenekan A
- Adenike O
- Adeolu AA
- Adetoye A
- Adewale B
- Adigun T
- Adolfsson A
- Aflori L
- Africander C
- Afshari A
- Agarwal V
- Agodirin O
- Aguero Vera M
- Aguzzoli C
- Ahmad A
- Ahmad N
- Ahmad T
- Ahmad T
- Ahmad Y
- Ahmed A
- Ahmed J
- Ai Y
- Aignatoaie M
- Aimoniotou-Georgiou E
- Akanmu O
- Akerman N
- Akinwale M
- Akring I
- Al 'Amri K
- Al Anizi M
- Al hussaini S
- Al-Soudaine Y
- Aladin H
- Alagbe-Briggs O
- Alaman Laguarda G
- Albaj S
- Alcock D
- Alcock L
- Aldecoa C
- Aldecoa C
- Aleixo FB
- Alexander H
- Alexander M
- Alexander-Sefre F
- Alherz F
- Ali H
- Ali M
- Ali S
- Alias A
- Alias AH
- Alias RLR
- Alit MA
- Allen S
- Allen SJ
- Allison J
- Alloj C
- Allorto NL
- Allsop J
- Almeida W
- Alonso E
- Alphonsus C
- Alvares D
- Alves MJ
- Amador JCB
- Ameer Y
- Amendola CP
- Ami N
- Amstrup-Hansen L
- Anagnou A
- Andersen C
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- Andreadaki E
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- Anillo V
- Antal O
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- Antonina W
- Apagaki E
- Apostolou K
- Applewhaite C
- Aquino LPG
- Ar P
- Arawwawala D
- Ardern D
- Argue R
- Arkalaki E
- Armaganidis A
- Armstrong J
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- Arnaoutoglou C
- Arnaoutoglou E
- Arnold G
- Arrandale L
- Arrich J
- Arrigo M
- Arshad H
- Arshad MA
- Arumugam S
- Arustei M
- Arvaniti K
- Arya A
- Arya S
- Asimakos A
- Asudo F
- Athanasakis E
- Atiku M
- Attrill E
- Attwood C
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- Avila Sanchez FJ
- Avila EJD
- Avis J
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- Ayyaz H
- Azam UAA
- Azambuja P
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- Aziz FZ
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- Baghirzada L
- Bai H-P
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- Bairaktari A
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- Balaji P
- Balajonda N
- Balanescu A
- Balasubramaniam M
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- Bali C
- Bandeira ME
- Banerjee R
- Bankewitz C
- Banner-Goodspeed V
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- Barcraft-Barnes H
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- 'Oxford University Press (OUP)'
- Publication date
- 01/01/2016
- Field of study
BACKGROUND: As global initiatives increase patient access to surgical treatments, there remains a need to understand the adverse effects of surgery and define appropriate levels of perioperative care. METHODS: We designed a prospective international 7-day cohort study of outcomes following elective adult inpatient surgery in 27 countries. The primary outcome was in-hospital complications. Secondary outcomes were death following a complication (failure to rescue) and death in hospital. Process measures were admission to critical care immediately after surgery or to treat a complication and duration of hospital stay. A single definition of critical care was used for all countries. RESULTS: A total of 474 hospitals in 19 high-, 7 middle- and 1 low-income country were included in the primary analysis. Data included 44Â 814 patients with a median hospital stay of 4 (range 2-7) days. A total of 7508 patients (16.8%) developed one or more postoperative complication and 207 died (0.5%). The overall mortality among patients who developed complications was 2.8%. Mortality following complications ranged from 2.4% for pulmonary embolism to 43.9% for cardiac arrest. A total of 4360 (9.7%) patients were admitted to a critical care unit as routine immediately after surgery, of whom 2198 (50.4%) developed a complication, with 105 (2.4%) deaths. A total of 1233 patients (16.4%) were admitted to a critical care unit to treat complications, with 119 (9.7%) deaths. Despite lower baseline risk, outcomes were similar in low- and middle-income compared with high-income countries. CONCLUSIONS: Poor patient outcomes are common after inpatient surgery. Global initiatives to increase access to surgical treatments should also address the need for safe perioperative care. STUDY REGISTRATION: ISRCTN5181700
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- Zhuang H
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- Zientara-Rytter K
- Zimmermann CM
- Ziviani E
- Zoladek T
- Zong W-X
- Zorov DB
- Zorzano A
- Zou W
- Zou Z
- Zou Z
- Zuryn S
- Zwerschke W
- Ălvarez ĂMC
- Ăvalos Y
- Ănal G
- ĂstĂŒn S
- ÄoliÄ M
- ÄokiÄ J
- Ćœerovnik E
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field
Myocyte membrane and microdomain modifications in diabetes: determinants of ischemic tolerance and cardioprotection
- Author
- A AdameovĂĄ
- A AdameovĂĄ
- A Alday
- A Barthel
- A Barua
- A Bilginoglu
- A Cavalli
- A Creus
- A Fujita
- A Gomez-Ruiz
- A Gysembergh
- A Görbe
- A Hassouna
- A Ibrahimi
- A Keszler
- A Kleindienst
- A Lopez-Izquierdo
- A Lumb
- A Makino
- A Malhotra
- A Nitenberg
- A Prasad
- A Rana
- A Ring
- A Tosaki
- A Tsang
- A Vecchini
- A Wichelhaus
- A Ziegelhöffer
- AA Bulhak
- AC Dimitriu-Leen
- AC Maritim
- AF Quest
- AJ Poe
- AN Mather
- AO Verkerk
- AR Busija
- AS Abdelmoneim
- AS Augustus
- AS Augustus
- AV Glukhov
- AW Cohen
- AW Cohen
- B Adams
- B Drenger
- B Giusti
- B Hissa
- B Laczy
- B Pani
- B Ziegelhöffer-Mihalovicovå
- BC Suh
- BD Rockett
- BF Peake
- BM Collins
- BW Doble
- BW Paty
- C Chen-Scarabelli
- C Csonka
- C Filippo Di
- C Hidalgo
- C Lengyel
- C Malfitano
- C Mineo
- C Moreno
- C Wunderlich
- C Zhou
- CA Makarewich
- CE Bennett
- CE Brom van den
- CE Heyliger
- CH Shao
- CJ Baier
- CJ Currie
- CJ Currie
- CJ Field
- CJ Knowles
- CJ Knowles
- CJ Lavie
- CK Roberts
- CN Antonescu
- D Ebel
- D Feuvray
- D Huang
- D Papahadjopoulos
- D Prisco
- D Sengupta
- D Zhao
- DA Brown
- DC Colayco
- DD Belke
- DD Belke
- DE Duzhyy
- DF Legler
- DJ Beech
- DJ Betteridge
- DJ Margolis
- DJ Paulson
- DJ Paulson
- DM Khodeer
- DM Roth
- DP Goel
- DS Park
- DW Hilgemann
- E Bakan
- E Balse
- E Camors
- E Kottenberg
- E Liepinsh
- E Lucas
- EA Sanidas
- EJ Anderson
- EJ Lee
- EJ Smart
- EM Balk
- EM Gaber
- ER Gross
- Eugene F. Du Toit
- F Carotenuto
- F Carotenuto
- F Elinder
- F Kehl
- F Tomai
- FB Hu
- FC Howarth
- FC Howarth
- FC Howarth
- FR Heinzel
- FS Hesari
- FW Booth
- G Barrientos
- G Biondi-Zoccai
- G GarcĂa-Cardeña
- G Gimpl
- G Hekimian
- G Luca De
- G Ma
- G Mieren Van der
- G Tse
- G Xu
- GA Colditz
- GA Ramirez-Correa
- GC Melkani
- GD Fairn
- GE Hoogslag
- GE Sumnicht
- GI Lancaster
- GJ Gross
- GN Pierce
- GN Pierce
- GW Barsness
- GW Cline
- H Ha
- H Kahleova
- H Li
- H Lin
- H Lin
- H Pareja-Galeano
- H Sano
- H Su
- H Su
- H Wang
- HA Eyre
- HA Jamieson
- Hemal H. Patel
- HH Patel
- HH Patel
- HH Patel
- HJ Whittington
- HK Saini
- HM Colhoun
- HM Medford
- HM Ruijter Den
- HN Fridolfsson
- HN Fridolfsson
- HN Yadav
- HP Lauritzen
- HR Hope
- HS Kim
- I Eitel
- I Levental
- I Levitan
- I Uphues
- I WaczulĂkovĂĄ
- J Bolk
- J Bossuyt
- J Fantini
- J Feng
- J Feng
- J Greaves
- J Greaves
- J Gustavsson
- J Hashimoto
- J Howie
- J Hu
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- T RavingerovĂĄ
- T Sato
- T Yamagishi
- TJ Kamp
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- TM Lee
- TO StĂžlen
- U Rajamani
- UA Ărom
- V Benediktsdottir
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- X Chen
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- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
International Consensus Statement on Rhinology and Allergy: Rhinosinusitis
- Author
- Aanaes K
- Abadie WM
- Abi Najm S
- Abigail P
- Abrahams JJ
- Abrass LJ
- Abreu CB
- Abreu NA
- Abshirini H
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- Agache I
- Aghamohammadi A
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- Ahn CN
- Ahn HJ
- Ahn JC
- Ahn SH
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- Aitken M
- Ajaiyeoba A
- Akbari E
- Akdis CA
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- Akiyama K
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- Al Komser MK
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- Alanin MC
- Albritton FDT
- Albu S
- Albu S
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- Aldini G
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- Allen EK
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- Amble FR
- American Academy of Pediatrics Steering Committee on Quality Improvement and Management (AAP SCQIM)
- Amin MR
- Amine M
- Amodu EJ
- Anamika A
- Anand V
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- Anderson WC
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- Ankichetty SP
- Annamalai S
- Anon JB
- AnselmoâLima WT
- Antisdel JL
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- Publication venue
- 'Wiley'
- Publication date
- 01/01/2021
- Field of study
Background: The 5 years since the publication of the first International Consensus Statement on Allergy and Rhinology: Rhinosinusitis (ICARâRS) has witnessed foundational progress in our understanding and treatment of rhinologic disease. These advances are reflected within the more than 40 new topics covered within the ICARâRSâ2021 as well as updates to the original 140 topics. This executive summary consolidates the evidenceâbased findings of the document. Methods: ICARâRS presents over 180 topics in the forms of evidenceâbased reviews with recommendations (EBRRs), evidenceâbased reviews, and literature reviews. The highest grade structured recommendations of the EBRR sections are summarized in this executive summary. Results: ICARâRSâ2021 covers 22 topics regarding the medical management of RS, which are grade A/B and are presented in the executive summary. Additionally, 4 topics regarding the surgical management of RS are grade A/B and are presented in the executive summary. Finally, a comprehensive evidenceâbased management algorithm is provided. Conclusion: This ICARâRSâ2021 executive summary provides a compilation of the evidenceâbased recommendations for medical and surgical treatment of the most common forms of RS
The effects of sildenafil on nerve regeneration in a rat model
- Publication venue
- German Medical Science GMS Publishing House; DĂŒsseldorf
- Publication date
- 05/10/2015
- Field of study
The effect of deep friction massage on pain, loss of strength and daily life skills of patients with lateral epicondylitis
- Publication venue
- German Medical Science GMS Publishing House; DĂŒsseldorf
- Publication date
- 05/10/2015
- Field of study
Outcomes of trochanteric femoral fractures treated with proximal femoral nail: an analysis of 100 consecutive cases
- Publication venue
- 'Dove Medical Press Ltd.'
- Publication date
- 01/04/2014
- Field of study
Mehmet Fatih Korkmaz,1 Mehmet Nuri Erdem,2 Zeliha Disli,3 Engin Burak Selcuk,4 Mustafa Karakaplan,1 Abdullah Gogus5 1Department of Orthopedics and Traumatology, Inonu University School of Medicine, Malatya, Turkey; 2Department of Orthopedics and Traumatology, Nisantasi University School of Medicine, Istanbul, Turkey; 3Department of Anesthesiology, Malatya Government Hospital, Malatya, Turkey; 4Department of Family Medicine, Inonu University School of Medicine, Malatya, Turkey; 5Department of Orthopedics and Traumatology, Florence Nightingale Hospital, Istanbul, Turkey Purpose: In this study, we aimed to report the results of a retrospective study carried out at our institute regarding cases of patients who had suffered proximal femoral fractures between January 2002 and February 2007, and who were treated with a proximal femoral nail. Materials and methods: One hundred consecutive cases were included in the study. A case documentation form was used to obtain intraoperative data including age, sex, mechanism of injury, type of fracture according to Association for Osteosynthesis/Association for the Study of Internal Fixation (AO/ASIF) classification and the American Society of Anesthesiologists' (ASA) physical status classification (ASA grade). Clinical and radiographic examinations were performed at the time of admission and at the 6th week; subsequent visits were organized on the 3rd month, 6th month, and 12th month, and in patients with longer follow-up and annually postoperatively. The Harris score of hip function was used, and any change in the position of the implants and the progress of the fracture union, which was determined radiologically, was noted. Results: The mean age of the patients was 77.66 years (range: 37–98 years), and the sex distribution was 32 males and 68 females. Seventy-three fractures were reduced by closed means, whereas 27 needed limited open reduction. The mean follow-up time for the study group was 31.3 months (range: 12–75 months). Postoperative radiographs showed a near-anatomical fracture reduction in 78% of patients. The Harris hip score was negatively correlated with the ASA score and patient age. No cases of implant failure were observed. Three patients died before discharge (one due to pulmonary embolism, two due to cardiac arrest), and five patients died due to unrelated medical conditions within the first 3 months of the follow-up. Conclusion: Our study showed that proximal femoral nail is a reliable fixation with good fracture union, and it is not associated with major complications in any type of trochanteric femoral fracture. Keywords: ASA, femoral fracture, Harris score, proximal femoral nai
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