Optical and mid-infrared line emission in nearby Seyfert galaxies

Line ratio diagnostics provide valuable clues on the source of ionizing radiation in galaxies with intense black hole accretion and starbursting events, such as local Seyfert or galaxies at the peak of the star formation history. We aim to provide a reference joint optical and mid-IR analysis for studying AGN identification via line ratios and testing predictions from photoionization models. We obtained homogenous optical spectra with the Southern Africa Large Telescope for 42 Seyfert galaxies with Spitzer/IRS spectroscopy and X-ray to mid-IR multiband data available. After confirming the power of the main optical ([OIII]) and mid-IR ([NeV], [OIV], [NeIII]) emission lines in tracing AGN activity, we explore diagrams based on ratios of optical and mid-IR lines by exploiting photoionization models of different ionizing sources (AGN, star formation and shocks). We find that pure AGN photoionization models are good at reproducing observations of Seyfert galaxies with an AGN fractional contribution to the mid-IR (5-40 micron) emission larger than 50 per cent. For targets with a lower AGN contribution these same models do not fully reproduce the observed mid-IR line ratios. Mid-IR ratios like [NeV]/[NeII], [OIV]/[NeII] and [NeIII]/[NeII] show a dependence on the AGN fractional contribution to the mid-IR unlike optical line ratios. An additional source of ionization, either from star formation or radiative shocks, can help explain the observations in the mid-IR. Among combinations of optical and mid-IR diagnostics in line ratio diagrams, only those involving the [OI]/Halpha ratio are promising diagnostics for simultaneously unraveling the relative role of AGN, star formation and, shocks. A proper identification of the dominant ionizing source would require the exploitation of analysis tools based on advanced statistical techniques as well as spatially resolved data.Comment: 31 pages, 15 figures, 2 tables. Accepted for publication in A&

Linking Self-Incompatibility, Dichogamy, and Flowering Synchrony in Two Euphorbia Species: Alternative Mechanisms for Avoiding Self-Fertilization?

Background: Plant species have several mechanisms to avoid selfing such as dichogamy or a self-incompatibility response. Dichogamy in a single flower may reduce autogamy but, to avoid geitonogamy, plants must show flowering synchronization among all their flowers (i.e. synchronous dichogamy). It is hypothesized that one species would not simultaneously show synchronous dichogamy and self-incompatibility because they are redundant mechanisms to reduce selfing; however, this has not been accurately assessed. Methodology/Principal Findings: This expectation was tested over two years in two natural populations of the closely related Mediterranean spurges Euphorbia boetica and E. nicaeensis, which completely avoid autogamy by protogyny at the cyathia level. Both spurges showed a high population synchrony (Z,79), and their inflorescences flower synchronously. In E. nicaeensis, there was no overlap among the cyathia in anthesis of successive inflorescence levels and the overlap between sexual phases of cyathia of the same inflorescence level was uncommon (4–16%). In contrast, E. boetica showed a high overlap among consecutive inflorescence levels (74–93%) and between sexual phases of cyathia of the same inflorescence level (48–80%). The flowering pattern of both spurges was consistent in the two populations and over the two successive years. A hand-pollination experiment demonstrated that E. nicaeensis was strictly self-compatible whereas E. boetica was partially self-incompatible. Conclusions/Significance: We propose that the complex pattern of synchronized protogyny in E. nicaeensis prevents geitonogamous crosses and, consequently, avoids selfing and inbreeding depression. In E. boetica, a high probability of geitonogamous crosses may occur but, alternatively, this plant escapes selfing through a self-incompatibility response. We posit that synchronous dichogamy and physiological self-incompatibility do not co-occur in the same species because each process is sufficiently effective in avoiding self-fertilization.España Ministerio de Ciencia y Tecnología PLO CGL2005-03731; CGL2008-02533-EEspaña Ministerio de Ciencia y Tecnología MA CGL2009-0825

Genetic diversity and population structure of indigenous chicken in Rwanda using microsatellite markers

[This corrects the article DOI: 10.1371/journal.pone.0225084.]

Does pre-dispersal seed predation limit reproduction and population growth in the alpine clonal plant Geum reptans?

We studied the impact of the seed damaging gall midge larva Geomyia alpina on its perennial alpine host plant Geum reptans. We analysed the effect of seed predation on reproduction by seeds, i.e. seed number, seed mass, and seed viability and on growth and clonal propagation of non-protected plants in comparison to plants protected from predation by an insecticide. Additionally, we assessed the consequences of seed predation for population growth using matrix projection modelling. Seed predation resulted in a decrease in total seed mass per flower head by 23.8% in non-protected plants (P > 0.05). Individual seed mass decreased with increasing infestation intensity (P > 0.05). Seed number remained unaffected because the sucking feeding behaviour by gall midge larvae does not evoke seed abortion. Percent germination of seeds from non-protected plants was reduced by 97.9% compared to seeds from protected plants. According to reduced seed viability, modelling revealed a decrease in population growth rate from lambda= 1.055 to lambda= 1.041. Predation did neither influence total plant biomass nor biomass fractions. But stolon dry-weight of non-protected plants increased by 24.1% (P > 0.05), which may indicate a trade-off between sexual reproduction and clonal propagation. Our results demonstrate that despite substantial reduction of viable seeds, predation by gall midge larvae only slightly affected population growth of G. reptans suggesting that in this alpine species, persistence by longevity and clonal propagation can balance potential seed losses by predation, at least for local population growth

Thermally Activated Delayed Fluorescence and Phosphorescence Quenching in Iminophosphonamide Copper and Zinc Complexes

The synthesis of copper and zinc complexes of four variably substituted iminophosphonamide ligands is presented. While the copper complexes form ligand-bridged dimers, the zinc compounds are monomeric. Due to different steric demand of the ligand the arrangement of the ligands within the dimeric complexes varies. Similar to the structurally related iminophosphonamide complexes of alkali metals and calcium, the steady-state and time-resolved photoluminescence (PL) of four of the seven compounds studied here as solids in a temperature range of 5–295 K can be described within the scheme of thermally activated delayed fluorescence (TADF). Accordingly, they exhibit bright blue-green phosphorescence at low temperatures (<100 K), which turns into delayed fluorescence by increasing the temperature. However, unusually, the fluorescence is practically absent in two copper complexes which otherwise still conform to the TADF scheme. In these cases, the excited singlet states decay essentially non-radiatively and their thermal population from the corresponding low-lying triplet states efficiently quenches PL (phosphorescence). Three other copper and zinc complexes only exhibit prompt fluorescence, evidencing a wide variation of photophysical properties in this class of compounds. The excited states of the copper complex with especially pronounced phosphorescence quenching were also investigated by low-temperature time-resolved infrared spectroscopy and quantum chemical calculations

Simple Synthetic Routes to Carbene‐M‐Amido (M=Cu, Ag, Au) Complexes for Luminescence and Photocatalysis Applications

The development of novel and operationally simple synthetic routes to carbene‐metal‐amido (CMA) complexes of copper, silver and gold relevant for photonic applications are reported. A mild base and sustainable solvents allow all reactions to be conducted in air and at room temperature, leading to high yields of the targeted compounds even on multigram scales. The effect of various mild bases on the N−H metallation was studied in silico and experimentally, while a mechanochemical, solvent‐free synthetic approach was also developed. Our photophysical studies on [M(NHC)(Cbz)] (Cbz=carbazolyl) indicate that the occurrence of fluorescent or phosphorescent states is determined primarily by the metal, providing control over the excited state properties. Consequently, we demonstrate the potential of the new CMAs beyond luminescence applications by employing a selected CMA as a photocatalyst. The exemplified synthetic ease is expected to accelerate the applications of CMAs in photocatalysis and materials chemistry

Total Bee Dependence on One Flower Species Despite Available Congeners of Similar Floral Shape

<div><p>Extreme specialization is a common phenomenon in antagonistic biotic interactions but it is quite rare in mutualistic ones. Indeed, bee specialization on a single flower species (monolecty) is a questioned fact. Here, we provide multiple lines of evidence on true monolecty in a solitary bee (<i>Flavipanurgus venustus</i>, Andrenidae), which is consistent across space (18 sites in SW Iberian Peninsula) and time (three years) despite the presence of closely related congeneric plant species whose flowers are morphologically similar. The host flower (<i>Cistus crispus</i>, Cistaceae) is in turn a supergeneralist, visited by at least 85 insect species. We uncover ultraviolet light reflectance as a distinctive visual cue of the host flower, which can be a key mechanism because bee specialization has an innate basis to recognize specific signals. Moreover, we hypothesized that a total dependence on an ephemeral resource (i.e. one flower species) must lead to spatiotemporal matching with it. Accordingly, we prove that the bee’s flight phenology is synchronized with the blooming period of the host flower, and that the densities of bee populations mirror the local densities of the host flower. This case supports the ‘predictable plethora’ hypothesis, that is, that host-specialization in bees is fostered by plant species providing predictably abundant floral resources. Our findings, along with available phylogenetic information on the genus <i>Cistus</i>, suggest the importance of historical processes and cognitive constraints as drivers of specialization in bee-plant interactions.</p></div