Enzimska ekstrakcija hidroksicinamičnih kiselina iz otpada nastalog pri proizvodnji kave

Ferulic, caffeic, p-coumaric and chlorogenic acids are classified as hydroxycinnamic acids, presenting anticarcinogenic, anti-inflammatory and antioxidant properties. In this work, enzymatic extraction has been studied in order to extract high value-added products like hydroxycinnamic acids from coffee pulp. A commercial pectinase and enzyme extract produced by Rhizomucor pusillus strain 23aIV in solid-state fermentation using olive oil or coffee pulp (CP) as an inducer of the feruloyl esterase activity were evaluated separately and mixed. The total content (covalently linked and free) of ferulic, caffeic, p-coumaric and chlorogenic acids was 5276 mg per kg of coffee pulp. Distribution was as follows (in %): chlorogenic acid 58.7, caffeic acid 37.6, ferulic acid 2.1 and p-coumaric acid 1.5. Most of the hydroxycinnamic acids were covalently bound to the cell wall (in %): p-coumaric acid 97.2, caffeic acid 94.4, chlorogenic acid 76.9 and ferulic acid 73.4. The content of covalently linked hydroxycinnamic acid was used to calculate the enzyme extraction yield. The maximum carbon dioxide rate for the solid-state fermentation using olive oil as an inducer was higher and it was reached in a short cultivation time. Nevertheless, the feruloyl esterase (FAE) activity (units per mg of protein) obtained in the fermentation using CP as an inducer was 31.8 % higher in comparison with that obtained in the fermentation using olive oil as the inducer. To our knowledge, this is the first report indicating the composition of both esterified and free ferulic, caffeic, p-coumaric and chlorogenic acids in coffee pulp. The highest yield of extraction of hydroxycinnamic acids was obtained by mixing the produced enzyme extract using coffee pulp as an inducer and a commercial pectinase. Extraction yields were as follows (in %): chlorogenic acid 54.4, ferulic acid 19.8, p-coumaric acid 7.2 and caffeic acid 2.3. An important increase in the added value of coffee pulp was mainly due to the extraction of chlorogenic acid.Ferulinska, kafeinska, p-kumarinska i klorogena kiselina pripadaju u hidroksicinamične kiseline, koje imaju antikarcinogena, protuupalna i antioksidativna svojstva. U radu je ispitana enzimska ekstrakcija hidroksicinamičnih kiselina iz otpada nastalog pri proizvodnji kave. Upotrijebljene su, zasebno i u smjesi, komercijalna pektinaza te enzimski esktrakt dobiven fermentacijom na čvrstoj podlozi od maslinovog ulja ili otpada nastalog pri proizvodnji kave (da bi se potaknula aktivnost feruloil esteraze), s pomoću Rhizomucor pusillus 23aIV. Ukupni je udio kovalentno vezanih i slobodnih hidroksicinamičnih kiselina bio 5276 mg/kg otpada. Od toga je bilo: 58,7 % klorogene; 37,6 % kafeinske; 2,1 % ferulinske i 1,5 % p-kumarinske kiseline. Većina je hidroksicinamičnih kiselina bila kovalentno vezana za staničnu stijenku, i to: 97,2 % p-kumarinske; 94,4 % kafeinske; 76,9 % klorogene i 73,4 % ferulinske kiseline. Pomoću udjela kovalentno vezanih hidroksicinamičnih kiselina izračunat je prinos ekstrahiranog enzima. Maksimalni je udio fermentacijom proizvedenog CO2 postignut na čvrstoj podlozi od maslinovog ulja, i to za kraće vrijeme nego na podlozi od otpada nastalog pri proizvodnje kave, iako je feruloil esteraza proizvedena primjenom ove druge podloge pokazala aktivnost veću za 31,8 %. Prema spoznajama autora ovo je prvo istraživanje u kojem su određeni udjeli vezanih i slobodnih hidroksicinamičnih kiselina u otpadu nastalom pri proizvodnji kave. Najveći je prinos ekstrakcije hidroksicinamičnih kiselina postignut primjenom komercijalne pektinaze i enzimskog ekstrakta. Pritom je izdvojeno 54,4 % klorogene; 19,8 % ferulinske; 7,2 % p-kumarinske i 2,3 % kafeinske kiseline, pa je zaključeno da je ovaj postupak dobar za ekstrakciju klorogene kiseline

Terahertz epsilon-near-zero graded-index lens

An epsilon-near-zero graded-index converging lens with planar faces is proposed and analyzed. Each perfectly-electric conducting (PEC) waveguide comprising the lens operates slightly above its cut-off frequency and has the same length but different cross-sectional dimensions. This allows controlling individually the propagation constant and the normalized characteristic impedance of each waveguide for the desired phase front at the lens output while Fresnel reflection losses are minimized. A complete theoretical analysis based on the waveguide theory and Fermat’s principle is provided. This is complemented with numerical simulation results of two-dimensional and three-dimensional lenses, made of PEC and aluminum, respectively, and working in the terahertz regime, which show good agreement with the analytical work.Effort sponsored by Spanish Government under contracts Consolider “Engineering Metamaterials” CSD2008-00066 and TEC2011-28664-C02-01. P.R.-U. is sponsored by the Government of Navarra under funding program “Formación de tecnólogos” 055/01/11. M.N.- C. is supported by the Imperial College Junior Research Fellowship. M. B. acknowledges funding by the Spanish Government under the research contract program Ramon y Cajal RYC-2011-08221. N.E. acknowledges the support from the US Office of Naval Research (ONR) Multidisciplinary University Research Initiatives (MURI) grant number N00014-10-1- 0942

EXPERIENCIA DEL PADECIMIENTO EN TRABAJADORES CON LUMBALGIA INESPECÍFICA CRÓNICA DERIVADA DE UN RIESGO DE TRABAJO

Conocer y describir la experiencia del padecimiento de los trabajadores portadores de Lumbalgia Inespecífica Crónica Derivada de un Riesgo de Trabajo (LICDRT). Entendiendo la experiencia del padecimiento como el fenómeno social subjetivo y dinámico en el cual los enfermos y sus allegados, organizan, interpretan y expresan un conjunto de sentimientos, estados de ánimo, sensaciones y cambios corporales, eventos ligados y/o derivados del padecimiento y de su atención, todo dentro de las estructuras, relaciones y significados sociales que lo determinan. El estudio emplea el método cualitativo desde la perspectiva de la teoría fundamentada de Anselm Strauss. Se realizaron entrevistas a profundidad semi-estructuradas a 6 pacientes del área metropolitana de Guadalajara, derechohabientes del Instituto Mexicano del Seguro Social. Los datos se analizaron mediante la metodología de la teoría fundamentada con el apoyo del programa Atlas ti 6. Los pacientes manifestaron su percepción de un sistema de seguridad deficiente, conflictos en la relación médico paciente, consistentes en falta de credibilidad por parte del personal médico hacia la sintomatología experimentada. Manifestaron que el principal síntoma fue dolor y limitación para manejo de cargas. Enfatizaron la necesidad de recibir información entendible acerca de las causas del dolor y evolución del padecimiento, así como trato serio y humano por parte del médico especialista. Sus sentimientos fueron de la tristeza, a la depresión y hasta pensamientos suicidas. Manifestaron apoyo de su familia y redes sociales. Expusieron una relación problemática con sus patrones y no hubo consecuencias económicas importantes. Abstract Understand and describe the illness experience of workers carrying nonspecific chronic low back pain resulting from an occupational hazard (LICDRT). Understanding the illness experience as the subjective and dynamic social phenomenon in which patients and their relatives, organize, interpret and express a set of feelings, moods, feelings and bodily changes, related events and / or derivatives of the disease and ttention, particularly whithin the structures, relationships and social meanings that determine. The study used the qualitative method from the perspective of grounded theory by Anselm Strauss. We conducted in-depth interviews, semi-structured to 6 patients in the metropolitan area of Guadalajara of the Mexican Institute of Social Security. The data were analyzed using grounded theory methodology, whit the support of software Atlas ti. Patients expressed their perception of poor security system, conflicts in the doctor-patient relationship, consisting of lack of credibility on the part of medical personnel to the symptoms experienced. They stated that the main symptom was pain and limitation for cargo handling. They emphasized the need for understandable information about the causes of pain and disease's development, as well as serious and humane treatment by the medical specialist. His feelings were sadness, depression and even suicidal thoughts. Expressed support of family and social networks. Presented a problematic relationship with their employers and no major economic consequences. Palabras clave: Lumbalgia Inespecífica crónica, riesgo de trabajo, estudio cualitativo, experiencia del padecimiento, teoría fundamentada, Chronic nonspecific low back pain

Influence of Femtosecond Laser Modification on Biomechanical and Biofunctional Behavior of Porous Titanium Substrates

[Abstract] Bone resorption and inadequate osseointegration are considered the main problems of titanium implants. In this investigation, the texture and surface roughness of porous titanium samples obtained by the space holder technique were modified with a femtosecond Yb-doped fiber laser. Different percentages of porosity (30, 40, 50, and 60 vol.%) and particle range size (100–200 and 355–500 μm) were compared with fully-dense samples obtained by conventional powder metallurgy. After femtosecond laser treatment the formation of a rough surface with micro-columns and micro-holes occurred for all the studied substrates. The surface was covered by ripples over the micro-metric structures. This work evaluates both the influence of the macro-pores inherent to the spacer particles, as well as the micro-columns and the texture generated with the laser, on the wettability of the surface, the cell behavior (adhesion and proliferation of osteoblasts), micro-hardness (instrumented micro-indentation test, P–h curves) and scratch resistance. The titanium sample with 30 vol.% and a pore range size of 100–200 μm was the best candidate for the replacement of small damaged cortical bone tissues, based on its better biomechanical (stiffness and yield strength) and biofunctional balance (bone in-growth and in vitro osseointegration).This research was funded by the Ministerio de Ciencia e Innovación del Gobierno de España (PID2019-109371GB-100), Junta de Andalucía (Spain), through the Project PAIDI P20-00671Junta de Andalucía; P20-0067

Narrowing the Genetic Causes of Language Dysfunction in the 1q21.1 Microduplication Syndrome

The chromosome 1q21.1 duplication syndrome (OMIM# 612475) is characterized by head anomalies, mild facial dysmorphisms, and cognitive problems, including autistic features, mental retardation, developmental delay, and learning disabilities. Speech and language development are sometimes impaired, but no detailed characterization of language problems in this condition has been provided to date. We report in detail on the cognitive and language phenotype of a child who presents with a duplication in 1q21.1 (arr[hg19] 1q21.1q21.2(145,764,455-147,824,207) × 3), and who exhibits cognitive delay and behavioral disturbances. Language is significantly perturbed, being the expressive domain the most impaired area (with significant dysphemic features in absence of pure motor speech deficits), although language comprehension and use (pragmatics) are also affected. Among the genes found duplicated in the child, CDH1L is upregulated in the blood of the proband. ROBO1, a candidate for dyslexia, is also highly upregulated, whereas, TLE3, a target of FOXP2, is significantly downregulated. These changes might explain language, and particularly speech dysfunction in the proband

Hospital admission and risk assessment associated to exposure of fungal bioaerosols at a municipal landfill using statistical models

The object of this research to determine the statistical relationship and degree of association between variables: hospital admission days and diagnostic (disease) potentially associated to fungal bioaerosols exposure. Admissions included acute respiratory infections, atopic dermatitis, pharyngitis and otitis. Statistical analysis was done using Statgraphics Centurion XVI software. In addition, was estimated the occupational exposure to fungal aerosols in stages of a landfill using BIOGAVAL method and represented by Golden Surfer XVI program. Biological risk assessment with sentinel microorganism A. fumigatus and Penicillium sp, indicated that occupational exposure to fungal aerosols is Biological action level. Preventive measures should be taken to reduce the risk of acquiring acute respiratory infections, dermatitis or other skin infections