New Longevity Record for Ivory Gulls (Pagophila eburnea) and Evidence of Natal Philopatry

Ivory gulls (Pagophila eburnea) have been listed as “endangered” in Canada and “near threatened” interna-tionally. In June 2010, we visited Seymour Island, Nunavut, Canada, where gulls were banded in the 1970s and 1980s. We recaptured and released two breeding gulls banded as chicks in 1983, confirming natal philopatry to this breeding colony. These gulls are more than 28 years old, making the ivory gull one of the longest-living marine bird species known in North America.La mouette blanche (Pagophila eburnea) figure sur la liste des espèces « en voie de disparition » sur la scène canadienne et des espèces « quasi menacées » sur la scène internationale. En juin 2010, nous sommes allés à l’île Seymour, au Nunavut, Canada, où des mouettes avaient été baguées dans le courant des années 1970 et 1980. Nous avons recapturé et relâché deux mouettes reproductrices qui étaient considérées comme des oisillons en 1983, ce qui nous a permis de confirmer la philopatrie natale de cette colonie de nidification. Ces mouettes blanches ont plus de 28 ans, ce qui en fait l’un des oiseaux aquatiques vivant le plus longtemps en Amérique du Nord

Local Ecological Knowledge of Ivory Gull Declines in Arctic Canada

We gathered local ecological knowledge (LEK) on the ivory gull (Pagophila eburnea) from residents of three High Arctic communities in eastern Canada. This gull has always been uncommon, but Inuit had suggested that numbers of gulls were declining. LEK from Grise Fiord and Resolute Bay was clear and consistent, indicating that far fewer gulls are currently observed near the community compared to 25 years ago. The LEK from Arctic Bay was less consistent, although in general, community members thought that the species was less commonly observed. Observations from nonsystematic surveys by local wildlife officers corroborated the LEK data, and an aerial survey of the known colony locations on the Brodeur Peninsula (near Arctic Bay) found only one gull. Collectively, this information suggests that ivory gull populations are declining across the species' Canadian range. Systematic surveys will be needed to confirm these perceived declines.On a recueilli du savoir écologique local (SEL) sur la mouette blanche (Pagophila eburnea) auprès de résidents de trois communautés de l'Extrême-Arctique situées dans l'est du Canada. Cette mouette a toujours été assez rare, mais, selon les Inuits, le nombre d'individus était en déclin. Le SEL de Grise Fiord et de Resolute Bay était clair et cohérent, révélant qu'actuellement, le nombre de mouettes observé près des communautés est bien plus faible qu'il y a 25 ans. Le SEL d'Arctic Bay était moins unanime, même si les membres de la communauté pensaient en général que l'espèce était aperçue moins fréquemment. Des observations tirées d'enquêtes non systématiques menées par les agents locaux de la faune corroboraient les données du SEL, et des relevés aériens des emplacements connus de colonies situées sur la presqu'île Brodeur (près d'Arctic Bay) n'ont repéré qu'une seule mouette. Ces renseignements conjugués suggèrent que la population de la mouette blanche est en baisse dans tout le territoire canadien de l'espèce. Des enquêtes systématiques seront nécessaires pour confirmer ce que l'on pense être un déclin

Complete breeding failures in ivory gull following unusual rainy storms in North Greenland

Natural catastrophic events such as heavy rainfall and windstorms may induce drastic decreases in breeding success of animal populations. We report the impacts of summer rainfalls on the reproductive success of ivory gull (Pagophila eburnea) in north-east Greenland. On two occasions, at Amdrup Land in July 2009 and at Station Nord in July 2011, we observed massive ivory gull breeding failures following violent rainfall and windstorms that hit the colonies. In each colony, all of the breeding birds abandoned their eggs or chicks during the storm. Juvenile mortality was close to 100% at Amdrup Land in 2009 and 100% at Station Nord in 2011. Our results show that strong winds associated with heavy rain directly affected the reproductive success of some Arctic bird species. Such extreme weather events may become more common with climate change and represent a new potential factor affecting ivory gull breeding success in the High Arctic

The free energy of NOAA active region AR 11029

The NOAA active region AR 11029 was a small but highly active sunspot region which produced 73 GOES soft X-ray flares. The flares appear to show a departure from the well known power-law frequency-size distribution. Specifically, too few GOES C-class and no M-class flares were observed by comparison with a power-law distribution (Wheatland in Astrophys. J. 710, 1324, 2010). This was conjectured to be due to the region having insufficient magnetic energy to power large events. We construct nonlinear force-free extrapolations of the coronal magnetic field of active region AR 11029 using data taken on 24 October by the SOLIS Vector-SpectroMagnetograph (SOLIS/VSM), and data taken on 27 October by the Hinode Solar Optical Telescope SpectroPolarimeter (Hinode/SP). Force-free modeling with photospheric magnetogram data encounters problems because the magnetogram data are inconsistent with a force-free model, and we employ a recently developed `self-consistency' procedure which addresses this and accommodates uncertainties in the boundary data (Wheatland and Regnier in Astrophys. J. 700, L88, 2009). We calculate the total energy and free energy of the self-consistent solution and find that the free energy was 4x10^29 erg on 24 October, and 7x10^31 erg on 27 October. An order of magnitude scaling between RHESSI non-thermal energy and GOES peak X-ray flux is established from a sample of flares from the literature and is used to estimate flare energies from observed GOES peak X-ray flux. Based on the scaling, we conclude that the estimated free energy of AR 11029 on 27 October when the flaring rate peaked is sufficient to power M-class or X-class flares, and hence the modeling does not appear to support the hypothesis that the absence of large flares is due to the region having limited energy.Comment: Accepted for publication in Solar Physic

Zoledronate in the prevention of Paget's (ZiPP) : protocol for a randomised trial of genetic testing and targeted zoledronic acid therapy to prevent SQSTM1-mediated Paget's disease of bone.

Introduction Paget’s disease of bone (PDB) is characterised by increased and disorganised bone remodelling affecting one or more skeletal sites. Complications include bone pain, deformity, deafness and pathological fractures. Mutations in sequestosome-1 (SQSTM1) are strongly associated with the development of PDB. Bisphosphonate therapy can improve bone pain in PDB, but there is no evidence that treatment alters the natural history of PDB or prevents complications. The Zoledronate in the Prevention of Paget’s disease trial (ZiPP) will determine if prophylactic therapy with the bisphosphonate zoledronic acid (ZA) can delay or prevent the development of PDB in people who carry SQSTM1 mutations. Methods and analysis People with a family history of PDB aged >30 years who test positive for SQSTM1 mutations are eligible to take part. At the baseline visit, participants will be screened for the presence of bone lesions by radionuclide bone scan. Biochemical markers of bone turnover will be measured and questionnaires completed to assess pain, health-related quality of life (HRQoL), anxiety and depression. Participants will be randomised to receive a single intravenous infusion of 5 mg ZA or placebo and followed up annually for between 4 and 8 years at which point baseline assessments will be repeated. The primary endpoint will be new bone lesions assessed by radionuclide bone scan. Secondary endpoints will include changes in biochemical markers of bone turnover, pain, HRQoL, anxiety, depression and PDB-related skeletal events

Hyperglycaemic conditions perturb mouse oocyte in vitro developmental competence via beta-O-linked glycosylation of Heat shock protein 90

STUDY QUESTION What is the effect of beta-O-linked glycosylation (O-GlcNAcylation) on specific proteins in the cumulus-oocyte complex (COC) under hyperglycaemic conditions? SUMMARY ANSWER Heat shock protein 90 (HSP90) was identified and confirmed as being O-GlcNAcylated in mouse COCs under hyperglycaemic conditions (modelled using glucosamine), causing detrimental outcomes for embryo development. WHAT IS KNOWN ALREADY O-GlcNAcylation of proteins occurs as a result of increased activity of the hexosamine biosynthesis pathway, which provides substrates for cumulus matrix production during COC maturation, and also for O-GlcNAcylation. COCs matured under hyperglycaemic conditions have decreased developmental competence, mediated at least in part through the mechanism of increased O-GlcNAcylation. STUDY DESIGN, SIZE, DURATION This study was designed to examine the effect of hyperglycaemic conditions (using the hyperglycaemic mimetic, glucosamine) on O-GlcNAc levels in the mouse COC, and furthermore to identify potential candidate proteins which are targets of this modification, and their roles in oocyte maturation. PARTICIPANTS/MATERIALS, SETTING, METHODS COCs from 21-day-old superovulated CBA × C57BL6 F1 hybrid female mice were matured in vitro (IVM). Levels of O-GlcNAcylated proteins, HSP90 and O-GlcNAc transferase (OGT, the enzyme responsible for O-GlcNAcylation) in COCs were measured using western blot, and localization observed using immunocytochemistry. For glycosylated HSP90 levels, and to test OGT-HSP90 interaction, immunoprecipitation was performed prior to western blotting. Embryo development was assessed using in vitro fertilization and embryo culture post-maturation. MAIN RESULTS AND THE ROLE OF CHANCE Addition of the hyperglycaemic mimetic glucosamine to IVM medium for mouse COCs increased detectable O-GlcNAcylated protein levels (by western blot and immunocytochemistry), and this effect was reversed using an OGT inhibitor (P < 0.05). HSP90 was identified as a target of O-GlcNAcylation in the COC, and inhibition of HSP90 during IVM reversed glucosamine-induced decreases in oocyte developmental competence (P < 0.05). We also demonstrated the novel finding of an association between HSP90 and OGT in COCs, suggesting a possible client–chaperone relationship. LIMITATIONS, REASONS FOR CAUTION In vitro maturation of COCs was used so that treatment time could be limited to the 17 h of maturation prior to ovulation. Additionally, glucosamine, a hyperglycaemic mimetic, was used because it specifically activates the hexosamine pathway which provides the O-GlcNAc moieties. The results in this study should be confirmed using in vivo models of hyperglycaemia and different HSP90 inhibitors. WIDER IMPLICATIONS OF THE FINDINGS This study leads to a new understanding of how diabetes influences oocyte competence and provides insight into possible therapeutic interventions based on inhibiting HSP90 to improve oocyte quality.L.A. Frank, M.L. Sutton-McDowall, H.M. Brown, D.L. Russell, R.B. Gilchrist, and J.G. Thompso

PROFIL WISATAWAN MUSEUM RADYA PUSTAKA SURAKARTA

Anggit Margaret, C9407031 2011. Profil Wisatawan Museum Radya Pustaka Surakarta. Program Studi Diploma III Usaha Perjalanan Wisata Fakultas Sastra Dan Seni Rupa Universitas Sebelas Maret Surakarta. Penelitian tugas akhir ini mengkaji tentang Profil Wisatawan di Museum Radya Pustaka Surakarta. Tujuan dari penelitian ini adalah untuk mengetahui dari daerah mana saja wisatawan yang berkunjung ke Museum Radya Pustaka, bagaimana ciri-ciri wisatawan yang berkunjung ke Museum Radya Pustaka serta harapan-harapan yang diinginkan wisatawan terhadap Museum Radya Pustaka. Penelitian dilakukan dengan metode kualitatif. Pengumpulan data dilakukan melalui wawancara dengan narasumber wisatawan yang berkujung di Museum Radya Pustaka Surakarta tempat penulis melakukan penelitian, serta studi pustaka dan studi dokumen guna menambah sumber data. Hasil penelitian menunjukkan bahwa (1) Sebagian besar wisatawan yang datang berasal dari Semarang sebesar 32%. (2) Mayoritas wisatawan yang berkunjung ke Museum Radya Pustaka berusia antara 17-25 tahun dan kebanyakan dari mereka adalah pelajar atau mahasiswa dengan prosentase 52%. (3) Sebagian besar wisatawan yang datang ke Museum Radya Pustaka adalah bertujuan untuk melakukan penelitian yaitu sebesar 34%. (4) Harapan wisatawan yang berkunjung terhadap kelangsungan Museum Radya Pustaka sebagian besar adalah agar ditingkatkan lagi pengelolaan dan keamanan museum, agar kejadian hilangnya benda-benda koleksi museum tidak terulang lagi dikemudian hari. Kesimpulan dari hasil penelitian ini bahwa wisatawan yang berkujung ke Museum Radya Pustaka Surakarta mayoritas berasal dari Semarang, mayoritas berusia 17-25 tahun dan kebanyakan dari mereka adalah berprofesi sebagai pelajar dan mahasiswa. Kebanyakan wisatawan yang datang bertujuan untuk melakukan penelitian, serta harapan wisatawan terhadap Museum Radya Pustaka adalah supaya lebih ditingkatkan lagi pengelolaan dan keamanan museum

Para-infectious brain injury in COVID-19 persists at follow-up despite attenuated cytokine and autoantibody responses

To understand neurological complications of COVID-19 better both acutely and for recovery, we measured markers of brain injury, inflammatory mediators, and autoantibodies in 203 hospitalised participants; 111 with acute sera (1–11 days post-admission) and 92 convalescent sera (56 with COVID-19-associated neurological diagnoses). Here we show that compared to 60 uninfected controls, tTau, GFAP, NfL, and UCH-L1 are increased with COVID-19 infection at acute timepoints and NfL and GFAP are significantly higher in participants with neurological complications. Inflammatory mediators (IL-6, IL-12p40, HGF, M-CSF, CCL2, and IL-1RA) are associated with both altered consciousness and markers of brain injury. Autoantibodies are more common in COVID-19 than controls and some (including against MYL7, UCH-L1, and GRIN3B) are more frequent with altered consciousness. Additionally, convalescent participants with neurological complications show elevated GFAP and NfL, unrelated to attenuated systemic inflammatory mediators and to autoantibody responses. Overall, neurological complications of COVID-19 are associated with evidence of neuroglial injury in both acute and late disease and these correlate with dysregulated innate and adaptive immune responses acutely