Boobytraps, valkuilen en instinkers in het burgerlijk recht

Voorwoord: Dit boek bevat dertien opstellen van masterstudenten van de Erasmus School of Law. Met het schrijven van hun bijdrage aan dit boek voltooiden zij – onder begeleiding van de redacteuren – hun Master Privaatrecht, Master Aansprakelijkheid en Verzekering of Togamaster. Het boek is alweer het zesde deel in de rij van scriptieboeken die de Rotterdamse sectie burgerlijk recht sinds 2007 heeft verzorgd. Na de titels ‘Privaatrecht ondersteund’ (2007), ‘Autonomie en paternalisme in het privaatrecht’ (2008), ‘Waar gehakt wordt...’ (2009), ‘Rake Remedies’ (2010) en ‘Fundamentele rechten en vermogensrechten’ (2011), is het nu tijd om de nieuwste generatie Rotterdamse civilisten aan het woord te laten over ‘Boobytraps, valkuilen en instinkers in het burgerlijk recht’. Wij maken van de gelegenheid gebruik om de masterstudenten te danken voor hun grote inzet. Een speciaal woord van dank zijn we verschuldigd aan Emma Krikke, die behulpzaam was bij het verzorgen van de teksten. Ook veel dank gaat uit naar Ann-Sophie Vandenberghe en Gerhard Wagner voor hun medewerking aan de groepsbijeenkomsten

Mesenchymal tumor organoid models recapitulate rhabdomyosarcoma subtypes

Rhabdomyosarcomas (RMS) are mesenchyme-derived tumors and the most common childhood soft tissue sarcomas. Treatment is intense, with a nevertheless poor prognosis for high-risk patients. Discovery of new therapies would benefit from additional preclinical models. Here, we describe the generation of a collection of 19 pediatric RMS tumor organoid (tumoroid) models (success rate of 41%) comprising all major subtypes. For aggressive tumors, tumoroid models can often be established within 4-8 weeks, indicating the feasibility of personalized drug screening. Molecular, genetic, and histological characterization show that the models closely resemble the original tumors, with genetic stability over extended culture periods of up to 6 months. Importantly, drug screening reflects established sensitivities and the models can be modified by CRISPR/Cas9 with TP53 knockout in an embryonal RMS model resulting in replicative stress drug sensitivity. Tumors of mesenchymal origin can therefore be used to generate organoid models, relevant for a variety of preclinical and clinical research questions

Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli

The use of carbon nanoparticles is shown for the detection and identification of different Shiga toxin-producing Escherichia coli virulence factors (vt1, vt2, eae and ehxA) and a 16S control (specific for E. coli) based on the use of lateral flow strips (nucleic acid lateral flow immunoassay, NALFIA). Prior to the detection with NALFIA, a rapid amplification method with tagged primers was applied. In the evaluation of the optimised NALFIA strips, no cross-reactivity was found for any of the antibodies used. The limit of detection was higher than for quantitative PCR (q-PCR), in most cases between 104 and 105 colony forming units/mL or 0.1–0.9 ng/μL DNA. NALFIA strips were applied to 48 isolates from cattle faeces, and results were compared to those achieved by q-PCR. E. coli virulence factors identified by NALFIA were in very good agreement with those observed in q-PCR, showing in most cases sensitivity and specificity values of 1.0 and an almost perfect agreement between both methods (kappa coefficient larger than 0.9). The results demonstrate that the screening method developed is reliable, cost-effective and user-friendly, and that the procedure is fast as the total time required is <1 h, which includes amplification

Mesenchymal tumor organoid models recapitulate rhabdomyosarcoma subtypes

Rhabdomyosarcomas (RMS) are mesenchyme-derived tumors and the most common childhood soft tissue sarcomas. Treatment is intense, with a nevertheless poor prognosis for high-risk patients. Discovery of new therapies would benefit from additional preclinical models. Here, we describe the generation of a collection of 19 pediatric RMS tumor organoid (tumoroid) models (success rate of 41%) comprising all major subtypes. For aggressive tumors, tumoroid models can often be established within 4–8 weeks, indicating the feasibility of personalized drug screening. Molecular, genetic, and histological characterization show that the models closely resemble the original tumors, with genetic stability over extended culture periods of up to 6 months. Importantly, drug screening reflects established sensitivities and the models can be modified by CRISPR/Cas9 with TP53 knockout in an embryonal RMS model resulting in replicative stress drug sensitivity. Tumors of mesenchymal origin can therefore be used to generate organoid models, relevant for a variety of preclinical and clinical research questions

Chat about what matters most: An analysis of chat contributions posted to an outpatient fertility website

Contains fulltext : 72837.pdf (publisher's version ) (Open Access)A content analysis of chat utterances generated by in vitro fertilization (IVF) patients and healthcare professionals revealed that most chat is about the treatment itself and not about childlessness; 56% discloses psychological aspects, 27% physical aspects, and 17% social aspects of the treatment; and that accounts of both external and internal coping behaviors could be identified

Outpatient participation in an interactive fertility website

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