Changes in dip and frictional properties of the basal detachment controlling orogenic wedge propagation and frontal collapse: the External central Betics case

Thin-skinned fold-and-thrust belts (FTBs) have been extensively studied through both field examples and modelling. The overall dynamics of FTBs are, therefore, well understood. One less understood aspect is the combined influence of across-strike changes in the detachment properties and the basement topography on the behaviour of an orogenic wedge. In this paper, we use field data together with reflection seismic interpretation from the External Zones of the Central Betics FTB, S Spain, to identify a significant increase in the wedge basal dip (a basement "threshold") coinciding with the pinch-out of a weak substrate. This induced both changes to the wedge geometry and to the basal friction, which in turn influenced the wedge dynamics. The changing dynamics led to a transient “stagnation” of the FTB propagation, topographic build-up and subsequent collapse of the FTB front. This in turn fed an important Langhian depocenter made up of mass transport deposits. Coevally with the FTB propagation, extension took place both parallel and perpendicular to the orogenic trend. This case study illustrates how across-strike changes in wedge basal properties can control the detailed behaviour of a developing FTB front, but questions remain regarding the time-space interaction and relative importance of the basal parameters

Autonomous on-board data processing and instrument calibration software for the SO/PHI

The extension of on-board data processing capabilities is an attractive option to reduce telemetry for scientific instruments on deep space missions. The challenges that this presents, however, require a comprehensive software system, which operates on the limited resources a data processing unit in space allows. We implemented such a system for the Polarimetric and Helioseismic Imager (PHI) on-board the Solar Orbiter (SO) spacecraft. It ensures autonomous operation to handle long command-response times, easy changing of the processes after new lessons have been learned and meticulous book-keeping of all operations to ensure scientific accuracy. This contribution presents the requirements and main aspects of the software implementation, followed by an example of a task implemented in the software frame, and results from running it on SO/PHI. The presented example shows that the different parts of the software framework work well together, and that the system processes data as we expect. The flexibility of the framework makes it possible to use it as a baseline for future applications with similar needs and limitations as SO/PHI.Comment: Conference: SPIE Astronomical Telescopes + Instrumentatio, Software and Cyberinfrastructure for Astronomy

SIRT2 Inhibition Rescues Neurodegenerative Pathology but Increases Systemic Inflammation in a Transgenic Mouse Model of Alzheimer’s Disease

Sirtuin 2 (SIRT2) has been proposed to have a central role on aging, inflammation, cancer and neurodegenerative diseases; however, its specific function remains controversial. Recent studies propose SIRT2 pharmacological inhibition as a therapeutic strategy for several neurodegenerative diseases including Alzheimer’s disease (AD). Surprisingly, none of these published studies regarding the potential interest of SIRT2 inhibition has assessed the peripheral adverse side consequences of this treatment. In this study, we demonstrate that the specific SIRT2 inhibitor, the compound 33i, does not exhibit genotoxic or mutagenic properties. Moreover, pharmacological treatment with 33i, improved cognitive dysfunction and long-term potentiation, reducing amyloid pathology and neuroinflammation in the APP/PS1 AD mouse model. However, this treatment increased peripheral levels of the inflammatory cytokines IL-1β, TNF, IL-6 and MCP-1. Accordingly, peripheral SIRT2 inhibition with the blood brain barrier impermeable compound AGK-2, worsened the cognitive capacities and increased systemic inflammation. The analysis of human samples revealed that SIRT2 is increased in the brain but not in the serum of AD patients. These results suggest that, although SIRT2 pharmacological inhibition may have beneficial consequences in neurodegenerative diseases, its pharmacological inhibition at the periphery would not be recommended and the systemic adverse side effects should be considered. This information is essential to maximize the therapeutic potential of SIRT2 inhibition not only for AD but also for other neurodegenerative diseases. Graphical Abstract: [Figure not available: see fulltext.]Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was funded by FEDER/Ministerio de Ciencia, Innovación y Universidades Agencia Estatal de Investigación/ Project (SAF2017-87595-R and PID2020-119729 GB-100)

Carcass and meat quality in light lambs in the segureña breed

Meat and carcass quality were studied in 124 Segureña breed lambs, 62 males and 62 females, slaughtered at the market weight, of 19-25 kg. Carcasses, with a yield between 48 percent (CCW/SW) and 55 percent (HCW/EW), were qualified for the most part in the 2 class of conformation as a fairly fatty, of 2/3 covered kidney and pink color muscle. There were differences among sexes for the fatness scores. The pelvic limb represents the 33.6 percent of the carcass, the shoulder 19.5 percent, the ribs+loin 17.7 percent, the breast 11.1 percent, the neck 7.3 percent, and the anterior ribs 6.7 percent; for commercial categories 58.1 percent pertained to the first category, 19.5 percent to the second and 19.8 percent to the third. Carcass were formed by 19.9 percent of fat, 52.7 percent muscle and 19.2 percent bone; these values show an ideal degree of fatness, in the local market. The sex had a significant effect on the percentage of fat and bone of the carcass. The average pH values, 6.6 and 6.5, following the preparation of the carcass for m. longissimus dorsi and m. triceps, decreased to 5.8 and 5.9, respectively, at 24 hours post mortem. These muscles presented a water holding capacity of 16.4 and 9.9 percent, and a tenderness of 3866 and 3945 g/cm2. The haem pigments and the color of m. longissimus dorsi and m. rectus abdominis confirm the results of its subjective evaluation.Se estudiaron características de calidad de la canal y de la carne en 124 corderos, 62 machos y 62 hembras, de raza Segureña sacrificados a peso de mercado, 19-25 kg. Las canales, con rendimientos entre el 48 p.100 (PCF/PVS) y el 55 p.100 (PCC/PVV), son de conformación normal (nota 2), medianamente grasas, de riñón cubierto 2/3 y músculo de color rosa; registrándose diferencias entre sexos para las calificaciones de engrasamiento. Al despiece, la pierna representó el 33,6 p.100 de la canal, la espalda el 19,5 p.100, el costillar el 17,7 p.100, los bajos el 11,1 p.100, el cuello el 7,3 p.100 y el badal el 6,7 p.100; lo que por categorías comerciales supone el 58,1 p.100 para las de 1ª, el 19,5 p.100 y 19,8 p.100 para las de 2ª y 3ª, respectivamente. La canal contenía 19,9 p.100 de grasa, 52,7 p.100 de músculo y 19,2 p.100 de hueso lo que muestra su buen grado de acabado. El sexo afectó significativamente al porcentaje de grasa y hueso de la canal. Los valores iniciales de pH, 6,6 y 6,3 para m. longissimus dorsi y m. triceps brachii, descienden a 5,8 y 5,9 a las 24 horas post mortem, respectivamente. La capacidad de retención de agua de estos músculos fue de 16,4 y 9,9 p.100 y su dureza media 3866 y 3945 g/cm2. El pigmento hemínico y el color de m. longissimus dorsi y m. rectus abdominis confirman la valoración subjetiva del color de la carne

Ten year outcomes of participants in the REACT (Randomised Evaluation of Assertive Community Treatment in North London) study

BackgroundA previous randomised controlled trial that investigated Assertive Community Treatment (ACT) in the UK (the REACT Study) found no clinical advantage over usual care delivered by Community Mental Health Teams (CMHTs) at 18 and 36 month follow-ups. No studies have investigated long term clinical and social outcomes for patients receiving ACT.MethodWe investigated inpatient service use, social outcomes, service contact and adverse events for the 251 REACT study participants 10 years after randomisation through case note review. Data were analysed using regression models adjusted for original treatment group allocation and changes in treatment group.ResultsWe found no statistically significant differences in outcomes by original treatment group over the 10 years. Those whose care remained with ACT, or transferred to ACT or forensic services, had more inpatient days over the 10 years (coefficient 223, 95% CI 83 to 363, p =0.002) than those whose care remained with the CMHTs or were discharged to primary care. Being subject to a Community Treatment Order was associated with a greater chance of being under ACT at 10 year follow-up (OR 6.39, 95% CI 2.98 to 13.70, p <0.001).ConclusionsThe ACT teams in this study showed no clinical advantage over usual care provided by CMHTs at 10 year follow-up. We also found that the ACT teams accrued patients from the original study sample who had more complex needs than those who remained with or transferred to the CMHTs or primary care during this period. Further well conducted trials are needed to identify the most cost-effective approaches to supporting successful community living and optimum long term outcomes for this group

Reference Gene Selection for Quantitative Real-time PCR Normalization in Quercus suber

The use of reverse transcription quantitative PCR technology to assess gene expression levels requires an accurate normalization of data in order to avoid misinterpretation of experimental results and erroneous analyses. Despite being the focus of several transcriptomics projects, oaks, and particularly cork oak (Quercus suber), have not been investigated regarding the identification of reference genes suitable for the normalization of real-time quantitative PCR data. In this study, ten candidate reference genes (Act, CACs, EF-1α, GAPDH, His3, PsaH, Sand, PP2A, ß-Tub and Ubq) were evaluated to determine the most stable internal reference for quantitative PCR normalization in cork oak. The transcript abundance of these genes was analysed in several tissues of cork oak, including leaves, reproduction cork, and periderm from branches at different developmental stages (1-, 2-, and 3-year old) or collected in different dates (active growth period versus dormancy). The three statistical methods (geNorm, NormFinder, and CV method) used in the evaluation of the most suitable combination of reference genes identified Act and CACs as the most stable candidates when all the samples were analysed together, while ß-Tub and PsaH showed the lowest expression stability. However, when different tissues, developmental stages, and collection dates were analysed separately, the reference genes exhibited some variation in their expression levels. In this study, and for the first time, we have identified and validated reference genes in cork oak that can be used for quantification of target gene expression in different tissues and experimental conditions and will be useful as a starting point for gene expression studies in other oaks

MeerKAT-64 discovers wide-spread tidal debris in the nearby NGC 7232 galaxy group

We report the discovery of large amounts of previously undetected cold neutral atomic hydrogen (H I) around the core triplet galaxies in the nearby NGC 7232 galaxy group with MeerKAT. With a physical resolution of ∼1 kpc, we detect a complex web of low-surface-brightness HI emission down to a 4σ column density level of ∼1 × 1019 cm-2 (over 44 km s-1). The newly discovered HI streams extend over ∼20 arcmin corresponding to 140 kpc in projection. This is approximately three times the HI extent of the galaxy triplet (52 kpc). The HI debris has an HI mass of ∼6.6 × 109 M⊙, more than 50 per cent of the total HI mass of the triplet. Within the galaxy triplet, NGC 7233 and NGC 7232 have lost a significant amount of HI while NGC 7232B appears to have an excess of HI. TheHI deficiency in NGC 7232 and NGC 7233 indicates that galaxÿCgalaxy interaction in the group concentrates on this galaxy pair while the other disc galaxies have visited them over time. In comparison to the AMIGA sample of isolated galaxies, we find that with regards to its total HI mass the NGC 7232/3 galaxy triplet is not HI-deficient. Despite the many interactions associated to the triplet galaxies, no HI seems to have been lost from the group (yet)

Reference Genes for Accurate Transcript Normalization in Citrus Genotypes under Different Experimental Conditions

Real-time reverse transcription PCR (RT-qPCR) has emerged as an accurate and widely used technique for expression profiling of selected genes. However, obtaining reliable measurements depends on the selection of appropriate reference genes for gene expression normalization. The aim of this work was to assess the expression stability of 15 candidate genes to determine which set of reference genes is best suited for transcript normalization in citrus in different tissues and organs and leaves challenged with five pathogens (Alternaria alternata, Phytophthora parasitica, Xylella fastidiosa and Candidatus Liberibacter asiaticus). We tested traditional genes used for transcript normalization in citrus and orthologs of Arabidopsis thaliana genes described as superior reference genes based on transcriptome data. geNorm and NormFinder algorithms were used to find the best reference genes to normalize all samples and conditions tested. Additionally, each biotic stress was individually analyzed by geNorm. In general, FBOX (encoding a member of the F-box family) and GAPC2 (GAPDH) was the most stable candidate gene set assessed under the different conditions and subsets tested, while CYP (cyclophilin), TUB (tubulin) and CtP (cathepsin) were the least stably expressed genes found. Validation of the best suitable reference genes for normalizing the expression level of the WRKY70 transcription factor in leaves infected with Candidatus Liberibacter asiaticus showed that arbitrary use of reference genes without previous testing could lead to misinterpretation of data. Our results revealed FBOX, SAND (a SAND family protein), GAPC2 and UPL7 (ubiquitin protein ligase 7) to be superior reference genes, and we recommend their use in studies of gene expression in citrus species and relatives. This work constitutes the first systematic analysis for the selection of superior reference genes for transcript normalization in different citrus organs and under biotic stress