Cosmological Parameter Determination in Free-Form Strong Gravitational Lens Modeling

We develop a novel statistical strong lensing approach to probe the cosmological parameters by exploiting multiple redshift image systems behind galaxies or galaxy clusters. The method relies on free-form mass inversion of strong lenses and does not need any additional information other than gravitational lensing. Since in free-form lensing the solution space is a high-dimensional convex polytope, we consider Bayesian model comparison analysis to infer the cosmological parameters. The volume of the solution space is taken as a tracer of the probability of the underlying cosmological assumption. In contrast to parametric mass inversions, our method accounts for the mass-sheet degeneracy, which implies a degeneracy between the steepness of the profile and the cosmological parameters. Parametric models typically break this degeneracy, introducing hidden priors to the analysis that contaminate the inference of the parameters. We test our method with synthetic lenses, showing that it is able to infer the assumed cosmological parameters. Applied to the CLASH clusters, the method might be competitive with other probes.Comment: 11 pages, 5 figures. Accepted for publication in MNRA

On the overconcentration problem of strong lensing clusters

Λ cold dark matter paradigm predicts that galaxy clusters follow a universal mass density profile and fit a well-defined mass-concentration relation, with lensing clusters being preferentially triaxial haloes elongated along the line of sight. Oddly, recent strong and weak lensing analyses of clusters with a large Einstein radius suggested those haloes to be highly overconcentrated. Here, we investigate what intrinsic shape and orientation a halo should have to account for both theoretical predictions and observations. We considered a sample of 10 strong lensing clusters. We first measured their elongation assuming a given mass-concentration relation. Then, for each cluster, we found the intrinsic shape and orientation which are compatible with the inferred elongation and the measured projected ellipticity. We distinguished two groups. The first one (nearly one-half) seems to be composed of outliers of the mass-concentration relation, which they would fit only if they were characterized by a filamentary structure extremely elongated along the line of sight, that is not plausible considering standard scenarios of structure formations. The second sample supports expectations of N-body simulations which prefer mildly triaxial lensing clusters with a strong orientation bia

On the over-concentration problem of strong lensing clusters

Lambda cold dark matter paradigm predicts that galaxy clusters follow an universal mass density profile and fit a well defined mass-concentration relation, with lensing clusters being preferentially triaxial haloes elongated along the line of sight. Oddly, recent strong and weak lensing analyses of clusters with a large Einstein radius suggested those haloes to be highly over-concentrated. Here, we investigate what intrinsic shape and orientation an halo should have to account for both theoretical predictions and observations. We considered a sample of 10 strong lensing clusters. We first measured their elongation assuming a given mass-concentration relation. Then, for each cluster we found the intrinsic shape and orientation which are compatible with the inferred elongation and the measured projected ellipticity. We distinguished two groups. The first one (nearly one half) seems to be composed of outliers of the mass-concentration relation, which they would fit only if they were characterised by a filamentary structure extremely elongated along the line of sight, that is not plausible considering standard scenarios of structure formations. The second sample supports expectations of N-body simulations which prefer mildly triaxial lensing clusters with a strong orientation bias.Comment: 11 pages, 8 figures, in press on MNRA

The Bi-Loop, a new general four-stranded DNA motif

The crystal structure of the cyclic octanucleotide d contains two independent molecules that form a novel quadruplex by means of intermolecular Watson-Crick A.T pairs and base stacking. A virtually identical quadruplex composed of G.C pairs was found by earlier x-ray analysis of the linear heptamer d(GCATGCT), when the DNA was looped in the crystal. The close correspondence between these two structures of markedly dissimilar oligonucleotides suggests that they are both examples of a previously unrecognized motif. Their nucleotide sequences have little in common except for two separated 5'-purine-pyrimidine dinucleotides forming the quadruplex, and by implication these so-called 'bi-loops' could occur widely in natural DNA. Such structures provide a mechanism for noncovalent linking of polynucleotides in vivo. Their capacity to associate by base stacking, demonstrated in the crystal structure of d(GCATGCT), creates a compact molecular framework made up of four DNA chains within which strand exchange could take place

Probing the dark matter issue in f(R)-gravity via gravitational lensing

For a general class of analytic f(R)-gravity theories, we discuss the weak field limit in view of gravitational lensing. Though an additional Yukawa term in the gravitational potential modifies dynamics with respect to the standard Newtonian limit of General Relativity, the motion of massless particles results unaffected thanks to suitable cancellations in the post-Newtonian limit. Thus, all the lensing observables are equal to the ones known from General Relativity. Since f(R)-gravity is claimed, among other things, to be a possible solution to overcome for the need of dark matter in virialized systems, we discuss the impact of our results on the dynamical and gravitational lensing analyses. In this framework, dynamics could, in principle, be able to reproduce the astrophysical observations without recurring to dark matter, but in the case of gravitational lensing we find that dark matter is an unavoidable ingredient. Another important implication is that gravitational lensing, in the post-Newtonian limit, is not able to constrain these extended theories, since their predictions do not differ from General Relativity.Comment: 7 pages, accepted for publication in EPJ

SCI1 Is a Direct Target of AGAMOUS and WUSCHEL and Is Specifically Expressed in the Floral Meristematic Cells

The specified floral meristem will develop a pre-established number of floral organs and, thus, terminate the floral meristematic cells. The floral meristematic pool of cells is controlled, among some others, by WUSCHEL (WUS) and AGAMOUS (AG) transcription factors (TFs). Here, we demonstrate that the SCI1 (Stigma/style cell-cycle inhibitor 1) gene, a cell proliferation regulator, starts to be expressed since the floral meristem specification of Nicotiana tabacum and is expressed in all floral meristematic cells. Its expression is higher in the floral meristem and the organs being specified, and then it decreases from outside to inside whorls when the organs are differentiating. SCI1 is co-expressed with N. tabacum WUSCHEL (NtWUS) in the floral meristem and the whorl primordia at very early developmental stages. Later in development, SCI1 is co-expressed with NAG1 (N. tabacum AG) in the floral meristem and specialized tissues of the pistil. In silico analyses identified cis-regulatory elements for these TFs in the SCI1 genomic sequence. Yeast one-hybrid and electrophoresis mobility shift assay demonstrated that both TFs interact with the SCI1 promoter sequence. Additionally, the luciferase activity assay showed that NAG1 clearly activates SCI1 expression, while NtWUS could not do so. Taken together, our results suggest that during floral development, the spatiotemporal regulation of SCI1 by NtWUS and NAG1 may result in the maintenance or termination of proliferative cells in the floral meristem, respectively.Fil: Cruz, Joelma O.. Universidade de Sao Paulo; BrasilFil: Abramo Barrera San Martin, Juca. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina. Universidade de Sao Paulo; BrasilFil: Lubini, Greice. Universidade de Sao Paulo; BrasilFil: Strini, Edward J.. Universidade de Sao Paulo; BrasilFil: Sobral, Rómulo. Universidade do Minho; PortugalFil: Pinoti, Vitor F.. Universidade de Sao Paulo; BrasilFil: Ferreira, Pedro B.. Universidade de Sao Paulo; BrasilFil: Thomé, Vanessa. Universidade de Sao Paulo; BrasilFil: Quiapim, Andréa C.. Universidade de Sao Paulo; BrasilFil: Dornelas, Marcelo C.. Universidade Estadual de Campinas; BrasilFil: Pranchevicius, Maria Cristina S.. Universidade Federal do São Carlos; BrasilFil: Madueño, Francisco. Consejo Superior de Investigaciones Científicas; EspañaFil: Costa, M. Manuela R.. Universidade do Minho; PortugalFil: Goldman, Maria Helena S.. Universidade de Sao Paulo; Brasi

A multi-wavelength strong lensing analysis of baryons and dark matter in the dynamically active cluster AC 114

Strong lensing studies can provide detailed mass maps of the inner regions even in dynamically active galaxy clusters. It is shown that proper modelling of the intracluster medium, i.e. the main baryonic component, can play an important role. In fact, the addition of a new contribution accounting for the gas can increase the statistical significance of the lensing model. We propose a parametric method for strong lensing analyses which exploits multi-wavelength observations. The mass model accounts for cluster-sized dark matter halos, galaxies (whose stellar mass can be obtained from optical analyses) and the intracluster medium. The gas distribution is fitted to lensing data exploiting prior knowledge from X-ray observations. This gives an unbiased look at each matter component and allows us to study the dynamical status of a cluster.}The method has been applied to AC 114, an irregular X-ray cluster. We find positive evidence for dynamical activity, with the dark matter distribution shifted and rotated with respect to the gas. On the other hand, the dark matter follows the galaxy density both for shape and orientation, which hints at its collisionless nature. The inner region (< 250kpc) is under-luminous in optical bands whereas the gas fraction (~20+- 5%) slightly exceeds typical values. Evidence from lensing and X-ray suggests that the cluster develops in the plane of the sky and is not affected by the lensing over-concentration bias. Despite the dynamical activity, the matter distribution seems to be in agreement with predictions from N-body simulations. An universal cusped profile provides a good description of either the overall or the dark matter distribution whereas theoretical scaling relations seem to be nicely fitted.Comment: 16 pages, 6 figures; v2 version: enhanced statistical analysis on the impact of gas, results unchanged; in press on A&

CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.

Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars

Molecular phylogeny and timing of diversification in Alpine Rhithrogena (Ephemeroptera: Heptageniidae).

BACKGROUND: Larvae of the Holarctic mayfly genus Rhithrogena Eaton, 1881 (Ephemeroptera, Heptageniidae) are a diverse and abundant member of stream and river communities and are routinely used as bio-indicators of water quality. Rhithrogena is well diversified in the European Alps, with a number of locally endemic species, and several cryptic species have been recently detected. While several informal species groups are morphologically well defined, a lack of reliable characters for species identification considerably hampers their study. Their relationships, origin, timing of speciation and mechanisms promoting their diversification in the Alps are unknown. RESULTS: Here we present a species-level phylogeny of Rhithrogena in Europe using two mitochondrial and three nuclear gene regions. To improve sampling in a genus with many cryptic species, individuals were selected for analysis according to a recent DNA-based taxonomy rather than traditional nomenclature. A coalescent-based species tree and a reconstruction based on a supermatrix approach supported five of the species groups as monophyletic. A molecular clock, mapped on the most resolved phylogeny and calibrated using published mitochondrial evolution rates for insects, suggested an origin of Alpine Rhithrogena in the Oligocene/Miocene boundary. A diversification analysis that included simulation of missing species indicated a constant speciation rate over time, rather than any pronounced periods of rapid speciation. Ancestral state reconstructions provided evidence for downstream diversification in at least two species groups. CONCLUSIONS: Our species-level analyses of five gene regions provide clearer definitions of species groups within European Rhithrogena. A constant speciation rate over time suggests that the paleoclimatic fluctuations, including the Pleistocene glaciations, did not significantly influence the tempo of diversification of Alpine species. A downstream diversification trend in the hybrida and alpestris species groups supports a previously proposed headwater origin hypothesis for aquatic insects