366 research outputs found
Pollen-pistil interactions and self-incompatibility in the Asteraceae: new insights from studies of Senecio squalidus (Oxford ragwort)
Background Pollen-pistil interactions are an essential prelude to fertilization in angiosperms and determine compatibility/incompatibility. Pollen-pistil interactions have been studied at a molecular and cellular level in relatively few families. Self-incompatibility (SI) is the best understood pollen-pistil interaction at a molecular level where three different molecular mechanisms have been identified in just five families. Here we review studies of pollen-pistil interactions and SI in the Asteraceae, an important family that has been relatively understudied in these areas of reproductive biology. Scope We begin by describing the historical literature which first identified sporophytic SI (SSI) in species of Asteraceae, the SI system later identified and characterized at a molecular level in the Brassicaceae. Early structural and cytological studies in these two families suggested that pollen-pistil interactions and SSI were similar, if not the same. Recent cellular and molecular studies in Senecio squalidus (Oxford ragwort) have challenged this belief by revealing that despite sharing the same genetic system of SSI, the Brassicaceae and Asteraceae molecular mechanisms are different. Key cellular differences have also been highlighted in pollen-stigma interactions, which may arise as a consequence of the Asteraceae possessing a ‘semi-dry' stigma, rather than the ‘dry' stigma typical of the Brassicaceae. The review concludes with a summary of recent transcriptomic analyses aimed at identifying proteins regulating pollen-pistil interactions and SI in S. squalidus, and by implication the Asteraceae. The Senecio pistil transcriptome contains many novel pistil-specific genes, but also pistil-specific genes previously shown to play a role in pollen-pistil interactions in other species. Conclusions Studies in S. squalidus have shown that stigma structure and the molecular mechanism of SSI in the Asteraceae and Brassicaceae are different. The availability of a pool of pistil-specific genes for S. squalidus offers an opportunity to elucidate the molecular mechanisms of pollen-pistil interactions and SI in the Asteracea
Evaluation of a 3-D rockfall module within a forest patch model
Many slopes in the Alps are prone to rockfall and forests play a vital role in protecting objects such as (rail) roads and infrastructure against rockfall. Decision support tools are required to assess rockfall processes and to quantify the rockfall protection effect of forest stands. This paper presents results of an iterative sequence of tests and improvements of a coupled rockfall and forest dynamics model with focus on the rockfall module. As evaluation data a real-size rockfall experiment in the French Alps and two 2-D rockfall trajectories from Austria and Switzerland were used. Modification of the rebound algorithm and the inclusion of an algorithm accounting for the sudden halt of falling rocks due to surface roughness greatly improved the correspondence between simulated and observed key rockfall variables like run-out distances, rebound heights and jump lengths for the real-size rockfall experiment. Moreover, the observed jump lengths and run-out distances of the 2-D trajectories were well within the stochastic range of variation yielded by the simulations. Based on evaluation results it is concluded that the rockfall model can be employed to assess the protective effect of forest vegetation
Genetic analysis of grape berries and raisins using microsatellite markers
Microsatellite markers have been used recently for the identification and pedigree analysis of grapevines with leaves and wood as sources of vine DNA. To identify grapes after harvest and their products, we applied DNA extraction protocols to grape berries and raisins. DNA was obtained from both sources, but that of raisins was highly degraded. The suitability of DNA for PCR amplification of single genetic loci was shown by amplification of 11 microsatellite markers. 18 commercially available table grape samples were genotyped, and 11 (61 %) matched the corresponding genetic profile in our reference database. Four samples were shown to be defined incorrectly and 4 samples did not match any of the generic profiles present in the database. The investigated raisins were found to be cv. Sultanina. The results demonstrate that DNA-based cultivar identification methods can be applied to harvested grapes and raisins
Fusion of two divergent fungal individuals led to the recent emergence of a unique widespread pathogen species
Multiple ITS Copies Reveal Extensive Hybridization within Rheum (Polygonaceae), a Genus That Has Undergone Rapid Radiation
During adaptive radiation events, characters can arise multiple times due to parallel evolution, but transfer of traits through hybridization provides an alternative explanation for the same character appearing in apparently non-sister lineages. The signature of hybridization can be detected in incongruence between phylogenies derived from different markers, or from the presence of two divergent versions of a nuclear marker such as ITS within one individual.In this study, we cloned and sequenced ITS regions for 30 species of the genus Rheum, and compared them with a cpDNA phylogeny. Seven species contained two divergent copies of ITS that resolved in different clades from one another in each case, indicating hybridization events too recent for concerted evolution to have homogenised the ITS sequences. Hybridization was also indicated in at least two further species via incongruence in their position between ITS and cpDNA phylogenies. None of the ITS sequences present in these nine species matched those detected in any other species, which provides tentative evidence against recent introgression as an explanation. Rheum globulosum, previously indicated by cpDNA to represent an independent origin of decumbent habit, is indicated by ITS to be part of clade of decumbent species, which acquired cpDNA of another clade via hybridization. However decumbent and glasshouse morphology are confirmed to have arisen three and two times, respectively.These findings suggested that hybridization among QTP species of Rheum has been extensive, and that a role of hybridization in diversification of Rheum requires investigation
Stress related epigenetic changes may explain opportunistic success in biological invasions in Antipode mussels
Different environmental factors could induce epigenetic changes, which are likely involved in the biological invasion process. Some of these factors are driven by humans as, for example, the pollution and deliberate or accidental introductions and others are due to natural conditions such as salinity. In this study, we have analysed the relationship between different stress factors: time in the new location, pollution and salinity with the methylation changes that could be involved in the invasive species tolerance to new environments. For this purpose, we have analysed two different mussels’ species, reciprocally introduced in antipode areas: the Mediterranean blue mussel Mytilus galloprovincialis and the New Zealand pygmy mussel Xenostrobus securis, widely recognized invaders outside their native distribution ranges. The demetylathion was higher in more stressed population, supporting the idea of epigenetic is involved in plasticity process. These results can open a new management protocols, using the epigenetic signals as potential pollution monitoring tool. We could use these epigenetic marks to recognise the invasive status in a population and determine potential biopollutants
Autocorrelation noise free Optical Coherence Tomography using the novel concept of resonant OCT (ROCT)
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