535 research outputs found
Chlamydia muridarum Genital and Gastrointestinal Infection Tropism Is Mediated by Distinct Chromosomal Factors
Some members of the genus Chlamydia, including the human pathogen Chlamydia trachomatis, infect multiple tissues, including the genital and gastrointestinal (GI) tracts. However, it is unknown if bacterial targeting to these sites is mediated by multifunctional or distinct chlamydial factors. We previously showed that disruption of individual large clostridial toxin homologs encoded within the Chlamydia muridarum plasticity zone were not critical for murine genital tract infection. Here, we assessed whether cytotoxin genes contribute to C. muridarum GI tropism. Infectivity and shedding of wild-type (WT) C. muridarum and three mutants containing nonsense mutations in different cytotoxin genes, tc0437, tc0438, and tc0439, were compared in mouse genital and GI infection models. One mutant, which had a nonsense mutation in tc0439, was highly attenuated for GI infection and had a GI 50% infectious dose (ID50) that was 1,000 times greater than that of the WT. GI inoculation with this mutant failed to elicit anti-chlamydial antibodies or to protect against subsequent genital tract infection. Genome sequencing of the tc0439 mutant revealed additional chromosomal mutations, and phenotyping of additional mutants suggested that the GI attenuation might be linked to a nonsense mutation in tc0600 The molecular mechanism underlying this dramatic difference in tissue-tropic virulence is not fully understood. However, isolation of these mutants demonstrates that distinct chlamydial chromosomal factors mediate chlamydial tissue tropism and provides a basis for vaccine initiatives to isolate chlamydia strains that are attenuated for genital infection but retain the ability to colonize the GI tract and elicit protective immune responses
Preface to the special issue of the Division Energy, Resources and the Environment at the EGU General Assembly EGU22
The European Geosciences Union (EGU) is one of the
leading global bottom-up societies that promotes earth, planetary and space
sciences. In its annual general assemblies, EGU brings together experts from
all over the world to discuss cutting-edge research and implementation of
findings in their respective disciplines and beyond via its inter-and
transdisciplinary sessions, and thus offers a unique forum for scientific
exchange, science-policy interaction, and joint development of strategies
for future research endeavours. Within that framework the Energy, Resources
and the Environment (ERE) Programme Group provides the platform for
discussion about adequate and reliable supplies of affordable energy and
other georesources in environmentally sustainable ways. This special issue
in Advances in Geosciences comprises a collection of contributions from the
ERE Programme Group, which were presented at the General Assembly 2022. It
was held in hybrid mode for the first time from 23–27 May 2022, after two
virtual assemblies in 2020 and 2021.</p
Mutational Analysis of the Chlamydia muridarum Plasticity Zone
Pathogenically diverse Chlamydia spp. can have surprisingly similar genomes. C. trachomatis isolates that cause trachoma, sexually transmitted genital tract infections (chlamydia) and invasive lymphogranuloma venereum (LGV), and the murine strain C. muridarum share 99% of their gene content. A region of high genomic diversity between Chlamydia spp. termed the Plasticity Zone (PZ) may encode niche-specific virulence determinants that dictate pathogenic diversity. We hypothesized that PZ genes might mediate the greater virulence and IFN-γ resistance of C. muridarum compared to C. trachomatis in the murine genital tract. To test this hypothesis, we isolated and characterized a series of C. muridarum PZ nonsense mutants. Strains with nonsense mutations in chlamydial cytotoxins, guaBA-add and a phospholipase D homolog developed normally in cell culture. Two of the cytotoxin mutants were less cytotoxic than wild-type suggesting that the cytotoxins may be functional. However, none of the PZ nonsense mutants exhibited increased IFN-γ sensitivity in cell culture or were profoundly attenuated in a murine genital tract infection model. Our results suggest that C. muridarum PZ genes are transcribed and some may produce functional proteins, but are dispensable for infection of the murine genital tract
Odd Frequency Pairing in the Kondo Lattice
We discuss the possibility that heavy fermion superconductors involve
odd-frequency pairing of the kind first considered by Berezinskii. Using a toy
model for odd frequency triplet pairing in the Kondo lattice we are able to
examine key properties of this new type of paired state. To make progress
treating the strong constraint in the Kondo lattice model we use the
technical trick of a Majorana representation of the local moments, which
permits variational treatments of the model without a Gutzwiller approximation.
The simplest mean field theory involves the development of bound states between
the local moments and conduction electrons, characterized by a spinor order
parameter. We show that this state is a stable realization of odd frequency
triplet superconductivity with surfaces of gapless excitations whose spin and
charge coherence factors vanish linearly in the quasiparticle energy. A
NMR relaxation rate coexists with a linear specific heat. We discuss possible
extensions of our toy model to describe heavy fermion superconductivity.Comment: 67 page
Expert Elicitation on Wind Farm Control
Wind farm control is an active and growing field of research in which the
control actions of individual turbines in a farm are coordinated, accounting
for inter-turbine aerodynamic interaction, to improve the overall performance
of the wind farm and to reduce costs. The primary objectives of wind farm
control include increasing power production, reducing turbine loads, and
providing electricity grid support services. Additional objectives include
improving reliability or reducing external impacts to the environment and
communities. In 2019, a European research project (FarmConners) was started
with the main goal of providing an overview of the state-of-the-art in wind
farm control, identifying consensus of research findings, data sets, and best
practices, providing a summary of the main research challenges, and
establishing a roadmap on how to address these challenges. Complementary to the
FarmConners project, an IEA Wind Topical Expert Meeting (TEM) and two rounds of
surveys among experts were performed. From these events we can clearly identify
an interest in more public validation campaigns. Additionally, a deeper
understanding of the mechanical loads and the uncertainties concerning the
effectiveness of wind farm control are considered two major research gaps
PENGARUH ATOPI TERHADAP TIMBULNYA DERMATITIS KONTAK PADA MAHASISWIRNPENDIDIKAN DOKTER FAKULTAS KEDOKTERAN UNIVERSITAS SYIAH KUALA RNBANDA ACEH
Dermatitis kontak adalah inflamasi non infeksi pada kulit yang disebabkan karena pemaparan dengan suatu zat tertentu yang dapat mengiritasi kulit atau menyebabkan reaksi alergi. Perempuan lebih sering mengalami dermatitis kontak dibandingkan laki-laki. Atopi merupakan salah satu faktor predisposisi timbulnya dermatitis kontak. Penelitian ini bertujuan untuk mengetahui pengaruh atopi terhadap timbulnya dermatitis kontak pada mahasiswi Pendidikan Dokter Fakultas Kedokteran Universitas Syiah Kuala Banda Aceh. Penelitian ini bersifat analitik dengan pendekatan cross sectional. Data yang digunakan adalah data primer yang diperoleh dengan wawancara langsung pada responden. Uji statistik dengan menggunakan Chi Square diperoleh nilai p yang signifikan sebesar 0,00, ini berarti p < 0,05 maka secara statistik didapatkan hubungan antara atopi dengan dermatitis kontak dengan rasio prevalensi 2,17, artinya seseorang yang menderita atopi memiliki peluang sebesar 2,17 kali untuk mengalami dermatitis kontak dibandingkan dengan orang yang tidak menderita atopi. Bahan yang paling sering menyebabkan dermatitis kontak pada mahasiswi adalah deterjen dan kosmetik. Hasil penelitian yang diperoleh ada pengaruh atopi terhadap timbulnya dermatitis kontak pada mahasiswi Pendidikan Dokter FK Unsyiah Banda Aceh, dimana mahasiswi yang menderita atopi cenderung mengalami dermatitis kontak.Kata kunci: Atopi, Dermatitis kontak Alergi, Dermatitis kontak iritanBanda Ace
Fabric Properties and Their Characteristics
This book describes the differences between woven and knitted structures, provides an understanding of fabric behavior and the characteristics of a functional garment, and outlines the importance of garment fit and consumer perception of ..
Non-Coding Changes Cause Sex-Specific Wing Size Differences between Closely Related Species of Nasonia
The genetic basis of morphological differences among species is still poorly understood. We investigated the genetic basis of sex-specific differences in wing size between two closely related species of Nasonia by positional cloning a major male-specific locus, wing-size1 (ws1). Male wing size increases by 45% through cell size and cell number changes when the ws1 allele from N. giraulti is backcrossed into a N. vitripennis genetic background. A positional cloning approach was used to fine-scale map the ws1 locus to a 13.5 kilobase region. This region falls between prospero (a transcription factor involved in neurogenesis) and the master sex-determining gene doublesex. It contains the 5′-UTR and cis-regulatory domain of doublesex, and no coding sequence. Wing size reduction correlates with an increase in doublesex expression level that is specific to developing male wings. Our results indicate that non-coding changes are responsible for recent divergence in sex-specific morphology between two closely related species. We have not yet resolved whether wing size evolution at the ws1 locus is caused by regulatory alterations of dsx or prospero, or by another mechanism. This study demonstrates the feasibility of efficient positional cloning of quantitative trait loci (QTL) involved in a broad array of phenotypic differences among Nasonia species
Blinatumomab vs historical standard therapy of adult relapsed/refractory acute lymphoblastic leukemia
We compared outcomes from a single-arm study of blinatumomab in adult patients with B-precursor Ph-negative relapsed/refractory acute lymphoblastic leukemia (R/R ALL) with a historical data set from Europe and the United States. Estimates of complete remission (CR) and overall survival (OS) were weighted by the frequency distribution of prognostic factors in the blinatumomab trial. Outcomes were also compared between the trial and historical data using propensity score methods. The historical cohort included 694 patients with CR data and 1112 patients with OS data compared with 189 patients with CR and survival data in the blinatumomab trial. The weighted analysis revealed a CR rate of 24% (95% CI: 20-27%) and a median OS of 3.3 months (95% CI: 2.8-3.6) in the historical cohort compared with a CR/CRh rate of 43% (95% CI: 36-50%) and a median OS of 6.1 months (95% CI: 4.2-7.5) in the blinatumomab trial. Propensity score analysis estimated increased odds of CR/CRh (OR=2.68, 95% CI: 1.67-4.31) and improved OS (HR=0.536, 95% CI: 0.394-0.730) with blinatumomab. The analysis demonstrates the application of different study designs and statistical methods to compare novel therapies for R/R ALL with historical data
Anemos : development of a next generation wind power forecasting system for the large-scale integration of onshore & offshore wind farms
International audienceThis paper presents the objectives and the research work carried out in the frame of the ANEMOS project on short-term wind power forecasting. The aim of the project is to develop accurate models that substantially outperform current state-of-the-art methods, for onshore and offshore wind power forecasting, exploiting both statistical and physical modeling approaches. The project focus on prediction horizons up to 48 hours ahead and investigates predictability of wind for higher horizons up to 7 days ahead useful i.e. for maintenance scheduling. Emphasis is given on the integration of highresolution meteorological forecasts. For the offshore case, marine meteorology is considered as well as information by satellite-radar images. An integrated software platform, ‘ANEMOS', is developed to host the various models. This system will be installed by several utilities for on-line operation at onshore and offshore wind farms for prediction at a local, regional and national scale. The applications include different terrain types and wind climates, on- and offshore cases, and interconnected or island grids. The on-line operation by the utilities will allow validation of the models and an analysis of the value of wind prediction for a competitive integration of wind energy in the developing liberalized electricity markets in the EU
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