Modeling human trophoblast, the placental epithelium at the maternal fetal interface.

Appropriate human trophoblast lineage specification and differentiation is crucial for the establishment of normal placentation and maintenance of pregnancy. However, due to the lack of proper modeling systems, the molecular mechanisms of these processes are still largely unknown. Much of the early studies in this area have been based on animal models and tumor-derived trophoblast cell lines, both of which are suboptimal for modeling this unique human organ. Recent advances in regenerative and stem cell biology methods have led to development of novel in vitro model systems for studying human trophoblast. These include derivation of human embryonic and induced pluripotent stem cells and establishment of methods for the differentiation of these cells into trophoblast, as well as the more recent derivation of human trophoblast stem cells. In addition, advances in culture conditions, from traditional two-dimensional monolayer culture to 3D culturing systems, have led to development of trophoblast organoid and placenta-on-a-chip model, enabling us to study human trophoblast function in context of more physiologically accurate environment. In this review, we will discuss these various model systems, with a focus on human trophoblast, and their ability to help elucidate the key mechanisms underlying placental development and function. This review focuses on model systems of human trophoblast differentiation, including advantages and limitations of stem cell-based culture, trophoblast organoid, and organ-on-a-chip methods and their applications in understanding placental development and disease

The theory of canonical perturbations applied to attitude dynamics and to the Earth rotation. Osculating and nonosculating Andoyer variables

The Hamiltonian theory of Earth rotation, known as the Kinoshita-Souchay theory, operates with nonosculating Andoyer elements. This situation parallels a similar phenomenon that often happens (but seldom gets noticed) in orbital dynamics, when the standard Lagrange-type or Delaunay-type planetary equations unexpectedly render nonosculating orbital elements. In orbital mechanics, osculation loss happens when a velocity-dependent perturbation is plugged into the standard planetary equations. In attitude mechanics, osculation is lost when an angular-velocity-dependent disturbance is plugged in the standard dynamical equations for the Andoyer elements. We encounter exactly this situation in the theory of Earth rotation, because this theory contains an angular-velocity-dependent perturbation (the switch from an inertial frame to that associated with the precessing ecliptic of date). While the osculation loss does not influence the predictions for the figure axis of the planet, it considerably alters the predictions for the instantaneous spin-axis' orientation. We explore this issue in great detail

Unaccompanied & Denied: Regional Legal Framework for Unaccompanied Minors Asylum Seekers (UMAS)

Unaccompanied minor asylum seekers are vulnerable and thus, provided special International law protections. However, in reality, they are being mistreated as illegal immigrants and on thereceiving end of ethnic violence, discrimination, restrictions in enjoyment of their rights duly recognised by International human rights law. This article identifies legislative, policy and supportmechanisms which encompass the minimum UMAS guardianship standards at International law and which are evidence-based from best practice models for the provision of guardians for UMASinternationally. It presents situation of UMAS in relation to human rights violations with emphasis on the legal framework and practices in Australia and five ASEAN State Members. This article also highlights the various stands taken by various countries providing better legal framework and practices regarding the terms for protection and enforcement of human rights for UMAS. Finally, this article provides recommendations for Australia and ASEAN Member States to adopt in order to realise the International human rights of UMAS with respect to guardianship

Methodological considerations in the analysis of fecal glucocorticoid metabolites in tufted capuchins (Cebus apella)

Analysis of fecal glucocorticoid (GC) metabolites has recently become the standard method to monitor adrenocortical activity in primates noninvasively. However, given variation in the production, metabolism, and excretion of GCs across species and even between sexes, there are no standard methods that are universally applicable. In particular, it is important to validate assays intended to measure GC production, test extraction and storage procedures, and consider the time course of GC metabolite excretion relative to the production and circulation of the native hormones. This study examines these four methodological aspects of fecal GC metabolite analysis in tufted capuchins (Cebus apella). Specifically, we conducted an adrenocorticotrophic hormone (ACTH) challenge on one male and one female capuchin to test the validity of four GC enzyme immunoassays (EIAs) and document the time course characterizing GC me- tabolite excretion in this species. In addition, we compare a common field-friendly technique for extracting fecal GC metabolites to an established laboratory extraction methodology and test for effects of storing “field extracts” for up to 1 yr. Results suggest that a corticosterone EIA is most sensitive to changes in GC production, provides reliable measures when extracted according to the field method, and measures GC metabolites which remain highly stable after even 12 mo of storage. Further, the time course of GC metabolite excretion is shorter than that described yet for any primate taxa. These results provide guidelines for studies of GCs in tufted capuchins, and underscore the importance of validating methods for fecal hormone analysis for each species of interest

A Study of the Orbits of the Logarithmic Potential for Galaxies

The logarithmic potential is of great interest and relevance in the study of the dynamics of galaxies. Some small corrections to the work of Contopoulos & Seimenis (1990) who used the method of Prendergast (1982) to find periodic orbits and bifurcations within such a potential are presented. The solution of the orbital radial equation for the purely radial logarithmic potential is then considered using the p-ellipse (precessing ellipse) method pioneered by Struck (2006). This differential orbital equation is a special case of the generalized Burgers equation. The apsidal angle is also determined, both numerically as well as analytically by means of the Lambert W and the Polylogarithm functions. The use of these functions in computing the gravitational lensing produced by logarithmic potentials is discussed.Comment: 12 pages, 4 figures. Accepted by MNRAS Sept 6 201

Orbits in Extended Mass Distributions: General Results and the Spirographic Approximation

This paper explores orbits in extended mass distributions and develops an analytic approximation scheme based on epicycloids (spirograph patterns). We focus on the Hernquist potential which provides a good model for many astrophysical systems, including elliptical galaxies, dark matter halos, and young embedded star clusters. For a given potential, one can readily calculate orbital solutions as a function of energy and angular momentum using numerical methods. In contrast, this paper presents a number of analytic results for the Hernquist potential and proves a series of general constraints showing that orbits have similar properties for any extended mass distribution (including, e.g., the NFW profile). We discuss circular orbits, radial orbits, zero energy orbits, different definitions of eccentricity, analogs of Kepler's law, the definition of orbital elements, and the relation of these orbits to spirograph patterns (epicycloids). Over much of parameter space the orbits can be adequately described (with accuracy better than 10%) using the parametric equations of epicycloids, thereby providing an analytic description of the orbits. As an application of this formal development, we find a solution for the orbit of the Large Magellanic Cloud in the potential of our Galaxy.Comment: 40 pages including 9 figures; accepted to The Astrophysical Journa

Self-gravitating warped discs around supermassive black holes

We consider warped equilibrium configurations for stellar and gaseous disks in the Keplerian force-field of a supermassive black hole, assuming that the self-gravity of the disk provides the only acting torques. Modeling the disk as a collection of concentric circular rings, and computing the torques in the non-linear regime, we show that stable, strongly warped precessing equilibria are possible. These solutions exist for a wide range of disk-to-black hole mass ratios $M_d/M_{bh}$, can span large warp angles of up to $\pm\sim 120\deg$, have inner and outer boundaries, and extend over a radial range of a factor of typically two to four. These equilibrium configurations obey a scaling relation such that in good approximation \phidot/\Omega\propto M_d/M_{bh} where \phidot is the (retrograde) precession frequency and $\Omega$ is a characteristic orbital frequency in the disk. Stability was determined using linear perturbation theory and, in a few cases, confirmed by numerical integration of the equations of motion. Most of the precessing equilibria are found to be stable, but some are unstable. The main result of this study is that highly warped disks near black holes can persist for long times without any persistent forcing other than by their self-gravity. The possible relevance of this to galactic nuclei is briefly discussed.Comment: 13 pages, 21 figures, published in MNRA