162 research outputs found
Human Elastase 1: Evidence for Expression in the Skin and the Identification of a Frequent Frameshift Polymorphism
Human pancreatic elastase 1 is a serine protease which maps to the chromosomal region 12q13 close to a locus for an autosomal dominant skin disease, diffuse nonepidermolytic palmoplantar keratoderma, and was investigated as a possible candidate gene for this disorder. Expression of two elastase inhibitors, elafin and SLPI, has been related to several hyperproliferative skin conditions. elastase 1 is functionally silent in the human pancreas but elastase 1 expression at the mRNA level was detected in human cultured primary keratinocytes. Antibody staining localized the protein to the basal cell layer of the human epidermis at a number of sites including the palmoplanta. Sequencing of genomic DNA from individuals with/without the keratoderma revealed a sequence variant, which would result in a premature truncation of the protein. This sequence variant, however, did not segregate with the skin disease and, indeed, was found to occur at a relatively high frequency in the population. Individuals homozygous for the variant do not have any obvious skin abnormalities. Based on the analysis of the secondary structure of the translated putative protein, the truncation is unlikely to result in knock-out of the elastase, but may cause destabilization of the enzyme–inhibitor complex
Örgüt Kültürü ve Örgüt İklimi Kavramlarının Kurumsallaşmış Hastanelerde Çalışan Ortopedi Uzman Doktor Karşılaştırması: Türkiye Örneği,
Sağlık sektörü içerisinde insan kaynakları bakımından
önemli yeri olan ve toplumla etkileşim halinde bulunan hastanelerin
uygun yönetim ilkeleriyle yönetilmeleri gerekmektedir.
Hastaneler oluşturdukları örgüt kültürü ve olumlu örgüt iklimi ile
daha kaliteli hizmet sunumu gerçekleştirebileceklerdir. Hastanelerde
örgüt kültürü ve buna bağlı örgüt ikliminin incelenmesi, kaliteli
hizmet sunumu ile örgüt performans ve verimliliğinin artırılmasına
yönelik çalışmalar için yol gösterici olabilir. Hastanelerde yoğun
birimlerden biri olan ortopedi bölümünde çalışan uzman doktorların
bazı demografik özelliklerinin hastanenin örgüt kültürü ve iklimine
ilişkin algıları üzerindeki etkilerinin incelenmesi çalışmanın temel
amacının oluşturmaktadır. Bu bağlamda 487 katılımcıya anket
uygulanmıştır. Katılımcılardan elde edilen anket verileri PASW
Statistic 18 paket programı ile analiz edilmiştir. Güvenirlilik analizi
sonucunda 0.934 Cronbach’s Alpha katsayısı elde edilmiştir. Elde
edilen verilerin incelenmesinde betimleyici istatistikler, güvenilirlik
analizi, ANOVA, Man-Whitney-U, Kruskal Wallis H testleri yapılmıştır.
Yapılan araştırma sonunda demografik değişkenlerin örgüt
kültürüne ilişkin örgütsel ahlak, örgütsel risk, örgütsel iş güvenliği,
örgütsel güven, örgütsel paylaşımcılık, örgütsel destek ve örgütsel
demografik birlik alt boyutlarında anlamlı farklıklar olduğu tespit
edilmiştir. Sonuç olarak bireylerin demografik özelliklerinin örgüt
kültürüne ilişkin algılarındaki farklılıkların tespit edilmesinin
örgütsel anlamda uygun strateji ve politikaların belirlenmesinde
önemli rol oynayacağı söylenebilir
Assessment of the effect of phenytoin on cutaneous healing from excision of melanocytic nevi on the face and on the back
<p>Abstract</p> <p>Background</p> <p>Topical phenytoin is a powerful skin wounds healing and it may be useful in clinical practice. The purpose of this study was to evaluate the effect of topical phenytoin 0.5%, by comparing it with cream (control) in wounds resulting from excision of two melanocytic nevi in the same patient. Our purpose was also to assess if phenytoin had better therapeutic and cosmetic outcomes when compared with cream (control).</p> <p>Methods</p> <p>This study evaluated 100 patients with skin wounds from excision of melanocytic nevi. 50 patients with lesions on the face and 50 patients with lesions on the back, totalizing 200 lesions excised with modified punch. The resulting superficial skin wounds had the same diameter and depth, and second intention healing followed.</p> <p>Patients were followed for 60 days. Student's t-test, Mann Whitney nonparametric test, analysis of variance, LSD test, Shapiro-Wilks test and Fisher test were used to analyze the results, depending on the nature of the variables being studied.</p> <p>Results</p> <p>Phenytoin showed better therapeutic and cosmetic results, by healing faster, with more intense epithelization in wounds in comparison with cream (control). Phenytoin showed a statistically significant difference regarding the following parameters (p < 0.05): wounded area and healing time. Phenytoin application resulted in a smaller area and a shorter healing time. Also the intensity of exudates, bleeding, and the epithelization were more intense in phenytoin-treated wounds. Regarding the shape and thickness of the scar, injuries treated with phenytoin had round and flat shaped scars in most of the cases. Considering patient's gender and phototype, female patients presented smaller wounds and scar areas; and phototype I had the largest scar areas. Contact eczema was an adverse reaction in 7 injuries located on the back caused by cream (control) and hypoallergenic tape.</p> <p>Conclusions</p> <p>Phenytoin showed better therapeutic and cosmetic results compared with cream (control). Phenytoin is a low cost drug, which accelerates skin wounds healing in human patients. Trial registration: ISRCTN96539803</p
Genetic Structure of Modern Durum Wheat Cultivars and Mediterranean Landraces Matches with Their Agronomic Performance
A collection of 172 durum wheat landraces from 21 Mediterranean countries and 20 modern cultivars were phenotyped in 6 environments for 14 traits including phenology, biomass, yield and yield components. The genetic structure of the collection was ascertained with 44 simple sequence repeat markers that identified 448 alleles, 226 of them with a frequency lower than 5%, and 10 alleles per locus on average. In the modern cultivars all the alleles were fixed in 59% of the markers. Total genetic diversity was HT = 0.7080 and the genetic differentiation value was GST = 0.1730. STRUCTURE software allocated 90.1% of the accessions in five subpopulations, one including all modern cultivars, and the four containing landrace related to their geographic origin: eastern Mediterranean, eastern Balkans and Turkey, western Balkans and Egypt, and western Mediterranean. Mean yield of subpopulations ranged from 2.6 t ha-1 for the western Balkan and Egyptian landraces to 4.0 t ha-1 for modern cultivars, with the remaining three subpopulations showing similar values of 3.1 t ha-1. Modern cultivars had the highest number of grains m-2 and harvest index, and the shortest cycle length. The diversity was lowest in modern cultivars (HT = 0.4835) and highest in landraces from the western Balkans and Egypt (HT = 0.6979). Genetic diversity and AMOVA indicated that variability between subpopulations was much lower (17%) than variability within them (83%), though all subpopulations had similar biomass values in all growth stages. A dendrogram based on simple sequence repeat data matched with the clusters obtained by STRUCTURE, improving this classification for some accessions that have a large admixture. landraces included in the subpopulation from the eastern Balkans and Turkey were separated into two branches in the dendrogram drawn with phenotypic data, suggesting a different origin for the landraces collected in Serbia and Macedonia. The current study shows a reliable relationship between genetic and phenotypic population structures, and the connection of both with the geographic origin of the landraces.The research was funded by the Ministerio de Economía y competitividad project AGL-2006-09226-C02-01, and Dr. Jose Miguel Soriano is funded by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (http://www.mineco.gob.es/)
Soil seed bank of the invasive Robinia pseudoacacia in planted Pinus nigra stands
Pinus nigra and Robinia pseudoacacia are exotic trees used for afforestation in Hungary. Pinus nigra was non-invasive, however R. pseudoacacia escaped from cultivation and invaded several vegetation types including pine plantations. It has recently been planned to cut P. nigra plantations and replace them by native tree stands, especially in nature reserves. The scattered presence of R. pseudoacacia specimens in pine stands might place constraints on planned tree replacement because of their vegetative resprouting
and recolonization from an established seed bank. The aim of this study was to investigate the soil seed bank under the canopy of solitary R. pseudoacacia specimens found in P. nigra plantations. Altogether 250 soil samples were collected from the 0–6 and 6–12 cm
soil layers under solitary Robinia trees of varying ages (with basal areas between 62.4 and 1089.3 cm2). Seeds were separated by sieving then scarified and germinated. Seed bank density ranged between 640 and 2285 seedsm–2 with an average distribution of 82.7% and 17.3% in the upper and lower soil layer, respectively. Total density of the seed bank and also the seed bank ratio of the lower soil layer increased with tree age. The accumulated seed bank of R. pseudoacacia should be considered in the careful planning of tree replacement operations in Pinus nigra stands
Natural Variation in Arabidopsis Cvi-0 Accession Reveals an Important Role of MPK12 in Guard Cell CO2 Signaling
Plant gas exchange is regulated by guard cells that form stomatal pores.
Stomatal adjustments are crucial for plant survival; they regulate
uptake of CO2 for photosynthesis, loss of water, and entrance of air
pollutants such as ozone. We mapped ozone hypersensitivity, more open
stomata, and stomatal CO2-insensitivity phenotypes of the Arabidopsis
thaliana accession Cvi-0 to a single amino acid substitution in
MITOGEN-ACTIVATED PROTEIN (MAP) KINASE 12 (MPK12). In parallel, we
showed that stomatal CO2-insensitivity phenotypes of a mutant cis
(CO2-insensitive) were caused by a deletion of MPK12. Lack of MPK12
impaired bicarbonate-induced activation of S-type anion channels. We
demonstrated that MPK12 interacted with the protein kinase HIGH LEAF
TEMPERATURE 1 (HT1)-a central node in guard cell CO2 signaling-and that
MPK12 functions as an inhibitor of HT1. These data provide a new
function for plant MPKs as protein kinase inhibitors and suggest a
mechanism through which guard cell CO2 signaling controls plant water
management.</p
Cardiotocography in breech versus vertex delivery: an examiner-blinded, cross-sectional nested case-control study
Effects of local phenytoin on seroma formation after mastectomy and Axillary lymph node dissection: an experimental study on mice
Natural Variation in Arabidopsis Cvi-0 Accession Reveals an Important Role of MPK12 in Guard Cell CO2 Signaling
Author Summary Human activities have increased the concentrations of CO2 and harmful air pollutants such as ozone in the troposphere. These changes can have detrimental consequences for agricultural productivity. Guard cells, which form stomatal pores on leaves, regulate plant gas exchange. To maintain photosynthesis, stomata open to allow CO2 uptake, but at the same time, open stomata lead to loss of water and allow the entrance of ozone. Elevated atmospheric CO2 levels reduce stomatal apertures, which can improve plant water balance but also increases leaf temperature. Using genetic approaches—in which we exploit natural variation and mutant analysis of thale cress (Arabidopsis thaliana)—we find that MITOGEN-ACTIVATED PROTEIN KINASE 12 (MPK12) and its inhibitory interaction with another kinase, HIGH LEAF TEMPERATURE 1 (HT1) (involved in guard cell CO2 signaling), play a key role in this regulatory process. We have therefore identified a mechanism in which guard cell CO2 signaling regulates how efficiently plants use water and cope with the air pollutant ozone.Peer reviewe
- …