Influence of storage time on essential oil components in dried hops

Hop processors often store fresh hops in silos prior to drying. There has been little knowledge regarding the effect of storage on hops. To investigate the effects on product quality, freshly harvested hop cones were stored for 5 and 24 hr respectively and dried for 210 min at 65°C thereafter. The results obtained from gas chromatography (GC) investigations show an increase in linalool, ß-carophyllen, humulen and geraniol content and decrease in myrcene content based on the period of storage

Percutaneous Anorectoplasty (PARP)-An Adaptable, Minimal-Invasive Technique for Anorectal Malformation Repair

Background: Anorectal malformations comprise a broad spectrum of disease. We developed a percutaneous anorectoplasty (PARP) technique as a minimal-invasive option for repair of amenable types of lesions. Methods: Patients who underwent PARP at five institutions from 2008 through 2021 were retrospectively analyzed. Demographic information, details of the operative procedure, and perioperative complications and outcomes were collected. Results: A total of 10 patients underwent the PARP procedure during the study interval. Patients either had low perineal malformations or no appreciable fistula. Most procedures were guided by ultrasound, fluoroscopy, or endoscopy. Median age at PARP was 3 days (range 1 to 311) days;eight patients were male. Only one intraoperative complication occurred, prompting conversion to posterior sagittal anorectoplasty. Functional outcomes in most children were highly satisfactory in terms of continence and functionality. Conclusions: The PARP technique is an excellent minimal-invasive alternative for boys born with perineal fistulae, as well as patients of both sexes without fistulae. The optimal type of guidance (ultrasound, fluoroscopy, or endoscopy) depends on the anatomy of the lesion and the presence of a colostomy at the time of repair

In-process investigation of the dynamics in drying behavior and quality development of hops using visual and environmental sensors combined with chemometrics

Hops are a key ingredient for beer brewing due to their role in preservation, the creation of foam characteristics, the bitterness and aroma of the beers. Drying significantly impacts on the composition of hops which directly affects the brewing quality of beers. Therefore, it is pivotal to understand the changes during the drying process to optimize the process with the central aim of improving product quality and process performance. Hops of the variety Mandarina Bavaria were dried at 65 °C and 70 °C with an air velocity of 0.35 m/s. Bulk weights investigated were 12, 20 and 40 kg/m2 respectively. Drying times were 105, 135, and 195 and 215 min, respectively. Drying characteristics showed a unique development, very likely due to the distinct physiology of hop cones (spindle, bracteole, bract, lupilin glands). Color changes depended strongly on the bulk weight and resulting bulk thickness (ΔE 9.5 (12 kg), 13 (20 kg), 18 (40 kg)) whilst α and ß acid contents were not affected by the drying conditions (full retention in all cases). The research demonstrated that specific air mass flow is critical for the quality of the final product, as well as the processing time required. Three types of visual sensors were integrated into the system, namely Vis-VNIR hyperspectral and RGB camera, as well as a pyrometer, to facilitate continuous in-process measurement. This enabled the dynamic characterization of the drying behavior of hops. Chemometric investigations into the prediction of moisture and chromatic information, as well as selected chemical components with full and a reduced wavelength set, were conducted. Moisture content prediction was shown to be feasible (r2 = 0.94, RMSE = 0.2) for the test set using 8 wavelengths. CIELAB a* prediction was also seen to be feasible (r2 = 0.75, RMSE = 3.75), alongside CIELAB b* prediction (r2 = 0.52 and RMSE = 2.66). Future work will involve possible ways to improve the current predictive models

Impact of Process Parameters and Bulk Properties on Quality of Dried Hops

Hops are critical to the brewing industry. In commercial hop drying, a large bulk of hops is dried in multistage kilns for several hours. This affects the drying behavior and alters the amount and chemical composition of the hop oils. To understand these changes, hops of the var. Hallertauer Tradition were dried in bulks of 15, 25 and 35 kg/m² at 60 °C and 0.35 m/s. Additionally, bulks of 25 kg/m² were also dried at 65 °C and 0.45 m/s to assess the effect of change in temperature and velocity, respectively. The results obtained show that bulk weights significantly influence the drying behavior. Classification based on the cone size reveals 45.4% medium cones, 41.2% small cones and 8.6% large cones. The highest ΔE value of 6.3 and specific energy consumption (113,476 kJ/kgH2O) were observed for the 15 kg/m² bulk. Increasing the temperature from 60 °C to 65 °C increased the oil yield losses by about 7% and myrcene losses by 22%. The results obtained show that it is important to define and consider optimum bulk and process parameters, to optimize the hop drying process to improve the process efficiency as well the product quality

Impact of Process Parameters and Bulk Properties on Quality of Dried Hops

Gefördert durch den Publikationsfonds der Universität Kasse

Google Glass for Documentation of Medical Findings: Evaluation in Forensic Medicine

Albrecht U-V, von Jan U, Kübler J, et al. Google Glass for Documentation of Medical Findings: Evaluation in Forensic Medicine. Presented at the Medicine 2.0. World Congress on Social Media, Mobile Apps, Internet / Web 2.0 in Health, Medicine and Biomedical Research, Malaga, Spain

Vznik fází a mechanické vlastnosti reaktivně a nereaktivně naprašovaných tvrdých vrstev Ti-B-N

Článek systematicky studuje vliv obsahu B ve vrstvách Ti-B-N připravených reaktivním i nereaktivním magnetronovým naprašováním. Rozdílné postupy depozice vedou na odlišné mikrostruktury a vazebné charakteristiky připravených vrstev. Během reaktivní depozice Ti-B-N dominují vazby B-N, zatímco vazby B-B a Ti-B dominují při nereaktivní depozici. Rentgenová difrakce a transmisní elektronová mikroskopie prokazují, že až 10 at.% B může být rozpuštěno v fcc mřížce Ti-N u vrstev připravených nereaktivním naprašováním. Na druhou stranu u vrstev připravených reaktivním naprašováním s obsahem B 4 at.% dochází již ke vzniku fáze BN.The article systematically studies the influence of B content on Ti-B-N during reactive as well as non-reactive DC magnetron sputtering. The different deposition routes allow for an additional, very effective key parameter to modify bond characteristics and microstructure. During reactive deposition of Ti-B-N, the B–N bonds dominate whereas the B–B and Ti–B bonds dominate for non-reactively prepared Ti-B-N. Detailed X-ray diffraction and transmission electron microscopy studies reveal, that up to 10 at.% B can be dissolved in the fcc-TiN lattice when prepared by non-reactive sputtering, whereas already for a B content of 4 at.% a BN-rich boundary phase forms when reactively sputtered

Human exome and mouse embryonic expression data implicate ZFHX3, TRPS1, and CHD7 in human esophageal atresia

Introduction: Esophageal atresia with or without tracheoesophageal fistula (EA/TEF) occurs approximately 1 in 3.500 live births representing the most common malformation of the upper digestive tract. Only half a century ago, EA/TEF was fatal among affected newborns suggesting that the steady birth prevalence might in parts be due to mutational de novo events in genes involved in foregut development. Methods: To identify mutational de novo events in EA/TEF patients, we surveyed the exome of 30 case-parent trios. Identified and confirmed de novo variants were prioritized using in silico prediction tools. To investigate the embryonic role of genes harboring prioritized de novo variants we performed targeted analysis of mouse transcriptome data of esophageal tissue obtained at the embryonic day (E) E8.5, E12.5, and postnatal. Results: In total we prioritized 14 novel de novo variants in 14 different genes (APOL2, EEF1D, CHD7, FANCB, GGT6, KIAA0556, NFX1, NPR2, PIGC, SLC5A2, TANC2, TRPS1, UBA3, and ZFHX3) and eight rare de novo variants in eight additional genes (CELSR1, CLP1, GPR133, HPS3, MTA3, PLEC, STAB1, and PPIP5K2). Through personal communication during the project, we identified an additional EA/TEF case-parent trio with a rare de novo variant in ZFHX3. In silico prediction analysis of the identified variants and comparative analysis of mouse transcriptome data of esophageal tissue obtained at E8.5, E12.5, and postnatal prioritized CHD7, TRPS1, and ZFHX3 as EA/TEF candidate genes. Re-sequencing of ZFHX3 in additional 192 EA/TEF patients did not identify further putative EA/TEF-associated variants. Conclusion: Our study suggests that rare mutational de novo events in genes involved in foregut development contribute to the development of EA/TEF