Modulatory Role of Sensory Innervation on Hair Follicle Stem Cell Progeny during Wound Healing of the Rat Skin

BACKGROUND: The bulge region of the hair follicle contains resident epithelial stem cells (SCs) that are activated and mobilized during hair growth and after epidermal wounding. However, little is known about the signals that modulate these processes. Clinical and experimental observations show that a reduced supply of sensory innervation is associated with delayed wound healing. Since axon terminals of sensory neurons are among the components of the bulge SC niche, we investigated whether these neurons are involved in the activation and mobilization of the hair stem cells during wound healing. METHODOLOGY/PRINCIPAL FINDINGS: We used neonatal capsaicin treatment to reduce sensory terminals in the rat skin and performed morphometric analyses using design-based stereological methods. Epithelial proliferation was analyzed by quantifying the number of bromodeoxyuridine-labeled (BrdU(+)) nuclei in the epidermis and hair follicles. After wounding, the epidermis of capsaicin-treated rats presented fewer BrdU(+) nuclei than in control rats. To assess SC progeny migration, we employed a double labeling protocol with iododeoxyuridine and chlorodeoxyuridine (IdU(+)/CldU(+)). The proportion of double-labeled cells was similar in the hair follicles of both groups at 32 h postwounding. IdU(+)/CldU(+) cell proportion increased in the epidermis of control rats and decreased in treated rats at 61 h postwounding. The epidermal volume immunostained for keratin 6 was greater in treated rats at 61 h. Confocal microscopy analysis revealed that substance P (SP) and calcitonin gene-related peptide (CGRP) receptor immunoreactivity were both present in CD34(+) and BrdU-retaining cells of the hair follicles. CONCLUSIONS/SIGNIFICANCE: Our results suggest that capsaicin denervation impairs SC progeny egress from the hair follicles, a circumstance associated with a greater epidermal activation. Altogether, these phenomena would explain the longer times for healing in denervated skin. Thus, sensory innervation may play a functional role in the modulation of hair SC physiology during wound healing

ONIX: Open Radio Network Information eXchange

While video-on-demand still takes up the lion's share of Internet traffic, we are witnessing a significant increase in the adoption of mobile applications defined by tight bit rate and latency requirements (e.g., augmented/virtual reality). Supporting such applications over a mobile network is very challenging due to the unsteady nature of the network and the long distance between the users and the application back-end, which usually sits in the cloud. To address these and other challenges, like security, reliability, and scalability, a new paradigm termed multi-access edge computing (MEC) has emerged. MEC places computational resources closer to the end users, thus reducing the overall end-to-end latency and the utilization of the network backhaul. However, to adapt to the volatile nature of a mobile network, MEC applications need real-time information about the status of the radio channel. The ETSI-defined radio network information service (RNIS) is in charge of providing MEC applications with up-to-date information about the radio network. In this article, we first discuss three use cases that can benefit from the RNIS (collision avoidance, media streaming, and Industrial Internet of Things). Then we analyze the requirements and challenges underpinning the design of a scalable RNIS platform, and report on a prototype implementation and its evaluation. Finally, we provide a roadmap of future research challenges

Interferometric characterization of the structured polarized light beam produced by the conical refraction phenomenon

The interest on the conical refraction (CR) phenomenon in biaxial crystals has revived in the last years due to its prospective for generating structured polarized light beams, i.e. vector beams. While the intensity and the polarization structure of the CR beams are well known, an accurate experimental study of their phase structure has not been yet carried out. We investigate the phase structure of the CR rings by means of a Mach-Zehnder interferometer while applying the phase-shifting interferometric technique to measure the phase at the focal plane. In general the two beams interfering correspond to different states of polarization (SOP) which locally vary. To distinguish if there is an additional phase added to the geometrical one we have derived the appropriate theoretical expressions using the Jones matrix formalism. We demonstrate that the phase of the CR rings is equivalent to that one introduced by an azimuthally segmented polarizer with CR-like polarization distribution. Additionally, we obtain direct evidence that the Poggendorff dark ring is an annular singularity, with a π phase change between the inner and outer bright rings.We acknowledge financial support from the Spanish MINECO and Fondos FEDER (FIS2012-39158-C02-01, FIS2011-23719, BES-2010-031696, and AP2010-2310), and the Catalan Government (2014 SGR 1639). C. Iemmi appreciates the support from UBACyT 20020100100689, CONICET PIP 112-200801-03047, and ANPCYT PICT 2010-02179 (Argentina)

Metodología dinámica de análisis de supervivencia aplicada a credit scoring

136 p.Esta investigación busca confeccionar una metodología que permita detectar y modelar cambios sufridos a lo largo del tiempo en los patrones que definen una clasificación en modelos de Análisis de Supervivencia. La Metodología Dinámica de Análisis de Supervivencia (MDAS) propuesta utiliza tres diferentes algoritmos de detección de cambios en los datos, con los cuales se entrenan modelos de Riesgos Proporcionales de Cox de manera independiente para luego seleccionar como modelo final aquel que minimice el error sobre un conjunto de prueba. La MDAS fue aplicada a un caso real de Credit Scoring con tres instancias de tiempo, y los resultados obtenidos fueron contrastados con los alcanzados por la metodología estática correspondiente con el fin de ilustrar la verdadera utilidad del método desarrollado. La metodología propuesta alcanzó una efectividad en promedio superior al modelo estático, lo cual fue estadísticamente comprobado. Por lo tanto, se logró desarrollar una metodología capaz de utilizar los datos más relevantes para el modelamiento del concepto objetivo de estudio. Adicionalmente, el Modelo de Riesgos Proporcionales de Cox ofrece una favorable interpretación de resultados que permitió analizar variables y aspectos relevantes en el riesgo de no pago. Palabras claves: Análisis de Supervivencia, Modelo de Riesgos Proporcionales de Cox, Metodología Dinámica, Credit Scoring. Abstract This research aims to make a methodology able to detect and model changes occurred along the time to the patterns which define a classification in Survival Analysis models. The called Dynamic Survival Analysis Methodology (MDAS) uses three different data change detection algorithms, which train Cox’s proportional hazards models independently and then selects as final model that one minimizes the global risk on a test set. The MDAS was applied over a real data of Credit Scoring with three time instances, and the outcomes achieved were contrasted versus the static methodology one, in order to show the real usefulness of the developed method. The proposed methodology achieved a higher average effectiveness than the static model, which was statistically proven. Therefore, it was possible to develop a methodology able to use the most relevant data to model the target. Moreover, the Cox’s proportional hazards model gives a useful interpretation of outcomes what allowed analyze characteristic variables and relevant issues in the default risk. Key words: Survival Analysis, Cox’s proportional hazards model, Dynamic Methodology, Credit Scoring

Compact LCOS–SLM Based Polarization Pattern Beam Generator

In this paper, a compact optical system for generating arbitrary spatial light polarization patterns is demonstrated. The system uses a single high-resolution liquid crystal (LC) on silicon (LCOS) spatial light modulator. A specialized optical mount is designed and fabricated using a 3D printer, in order to build a compact dual optical architecture, where two different phase patterns are encoded on two adjacent halves of the LCOS screen, with a polarization transformation in between. The final polarization state is controlled via two rotations of the Poincaré sphere. In addition, a relative phase term is added, which is calculated based on spherical trigonometry on the Poincaré sphere. Experimental results are presented that show the effectiveness of the system to produce polarization patterns

Changes in Alcohol Use during the COVID-19 Pandemic among Young Adults: The Prospective Effect of Anxiety and Depression

Versión editorHealth measures instantiated to reduce the spread of COVID-19 have imposed significant constraints for the population and impacted on drinking habits and mental health. This study longitudinally compared changes in alcohol consumption before and after the COVID-19 outbreak and the impact of sociodemographic and mental health variables on such changes among a community sample of young adults. Data were collected in the context of a larger, ongoing longitudinal study. The sample consisted of 305 young adults from Spain aged between 18 and 26 years (mean age = 21.27, (SD = 2.21), female = 53.4%; college students = 61.6%) who completed first (November-2019 and February-2020; i.e., before the outbreak of COVID-19) and second follow-up questionnaires (March 2021, a year after the COVID-19 outbreak). Alcohol use (quantity and drinking frequency), depression and anxiety symptoms were measured. Quantity and frequency of alcohol use decreased from the preto post-COVID-19 period. A decrease in drinking frequency was observed among college students, but not in noncollege peers. Although we found no effect of pre-COVID-19 anxiety on alcohol use changes, those with more depressive symptoms at the pre-COVID assessment were more resistant to decreasing their drinking quantity and frequency after the COVID-19 outbreak. This information will be of value when designing interventions aimed at reducing harmful alcohol use and highlights the role of mental health status when identifying high risk populations of young-adults during this, and future, public health crisesFunding for this study was provided by the Consejería de Salud (Junta de Andalucía, Andalucía, Spain) under Grant Number PI-0503-2018 (Principal Investigator: Fermín Fernández Calderón). BV was supported by Fundación Carolina and SEGIB, and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET

Characterization of interstitial cells of Cajal in bowel of cattle (Bos taurus)

Interstitial cells of Cajal (ICC) have been described in the gastrointestinal tract of different mammals including humans, horses, pigs, rats, dogs, mice and guinea-pigs. In the present study, ICC were identified in the jejunum of Bos taurus using polyclonal anti-c-Kit antibodies in immunohistochemical assays. Vimentin and desmin intermediate filaments were also determined using monoclonal antibodies. ICC were found in the tunica muscularis either in a palisade distribution pattern between the outer longitudinal and the inner circular layers (ICC-MP) or freely distributed in clusters in the longitudinal layer (ICC-LM). Morphometric studies determined that ICC have a fusiform shape presenting cytoplasmic prolongations. ICC were positive to c-Kit and vimentin antigens but negative to desmin. We have observed and described for the first time the presence of ICC in a ruminant. As observed in the aforementioned mammals, bovine ICC were associated with the myenteric plexus. Nevertheless, the presence of widespread ICC in the longitudinal muscular layer of the jejunum differs from previously described studies of other mammals.Facultad de Ciencias Veterinaria

Factores de rendimiento asociados a SPMD

Actualmente existen muchas aplicaciones paralelas/distribuidas en las cuales SPMD es el paradigma más usado. Obtener un buen rendimiento en una aplicación paralela de este tipo es uno de los principales desafíos dada la gran cantidad de aplicaciones existentes. Este objetivo no es fácil de resolver ya que existe una gran variedad de configuraciones de hardware, y también la naturaleza de los problemas pueden ser variados así como la forma de implementarlos. En consecuencia, si no se considera adecuadamente la combinación "software/hardware" pueden aparecer problemas inherentes a una aplicación iterativa sin una jerarquía de control definida de acuerdo a este paradigma. En SPMD todos los procesos ejecutan el mismo código pero computan una sección diferente de los datos de entrada. Una solución a un posible problema del rendimiento es proponer una estrategia de balance de carga para homogeneizar el cómputo entre los diferentes procesos. En este trabajo analizamos el benchmark CG con cargas heterogéneas con la finalidad de detectar los posibles problemas de rendimiento en una aplicación real. Un factor que determina el rendimiento en esta aplicación es la cantidad de elementos nonzero contenida en la sección de matriz asignada a cada proceso. Determinamos que es posible definir una estrategia de balance de carga que puede ser implementada de forma dinámica y demostramos experimentalmente que el rendimiento de la aplicación puede mejorarse de forma significativa con dicha estrategia.There currently are many 'parallel/distributed' applications that use the SPMD paradigm. Getting a good performance in a parallel application of this type is a major challenge because of the large number of existing applications. This objective is not easily achieved because there are many hardware configurations possible, and also the nature of the problems can be varied as well as its implementation. Consequently, if not adequately consider the combination 'software/hardware' inherent problems can occur without an iterative application defined control hierarchy according to this paradigm. In SPMD all processes execute the same code but they compute a different section of the input data. In this paper we analyze the benchmark CG with heterogeneous loads in order to detect possible performance problems in a real application. One factor that determines the performance in this application is the number of elements nonzero contained in the array section assigned to each process. We determined that it is possible to define a load balancing strategy, which can be implemented dynamically, and we demonstrate experimentally that the application performance can be significantly improved with this approach.Actualment existeixen moltes aplicacions paral·leles/distribuïdes en les quals SPMD és el paradigma més emprat. Obtenir un bon rendiment en una aplicació paral·lela d'aquest tipus és un dels principals reptes donada la gran quantitat d'aplicacions existents. Aquest objectiu no és fàcil de resoldre donat que existeixen una gran varietat de configuracions de hardware, i també la naturalesa dels problemes pot ser variada així com la forma d'implementar-los. En conseqüència, si no es considera adequadament la combinació "software/hardware" poden aparèixer problemes inherents a una aplicació iterativa sense una jerarquia de control definida d'acord a aquest paradigma. En SPMD tots els processos executen el mateix codi però computen una secció diferent de les dades d'entrada. Una solució a un possible problema de rendiment es proposar una estratègia de balanceig de càrrega per homogeneïtzar el còmput entre els diferents processos. En aquest treball analitzem el benchmark CG amb càrregues heterogènies amb la finalitat de detectar els possibles problemes de rendiment en una aplicació real. Un factor que determina el rendiment en aquesta aplicació és la quantitat d'elements nonzero continguda en la secció de la matriu assignada a cada procés. Es determina que és possible definir una estratègia de balanceig de càrrega que pot ser implementada de forma dinàmica i es demostra de forma experimental que el rendiment de la aplicació pot millorar-se de forma significativa amb aquesta estratègia

Planificación patrimonial : una mirada desde el territorio al patrimonio cultural

Fil: Grizas, Eduardo. Universidad Nacional de San JuanFil: Gnecco, María Julia. Universidad Nacional de San JuanFil: Arias, Daniel. Universidad Nacional de San JuanFil: Vera, Claudio. Universidad Nacional de San JuanFil: Márquez, Stella. Universidad Nacional de San JuanFil: Villavicencio, María Verónica. Universidad Nacional de San Jua

Hydrogen production and degradation of ciprofloxacin by Ag@TiO2-MoS2 Photocatalysts

The photocatalytic activity of silver-based catalysts containing different amounts of molybdenum disulfide (MoS2; 5, 10 and 20 wt.%) was evaluated by the degradation of the antibiotic ciprofloxacin and the production of hydrogen via water splitting. All the silver (Ag)-based catalysts degraded more than 70% of the antibiotic in 60 min. The catalyst that exhibited the best result was 5%Ag@TiO2-P25-5%MoS2, with ca. 91% of degradation. The control experiments and stability tests showed that photocatalysis was the degradation pathway and the selected silver-based catalysts were stable after seven cycles, with less than 2% loss of efficiency per cycle and less than 7% after seven cycles. The catalyst with the highest hydrogen production was 5%Ag@TiO2 NWs-20%MoS2, 1792 μmol/hg, at a wavelength of 400 nm. This amount was ca. 32 times greater than that obtained by the pristine titanium oxide nanowires catalyst. The enhancement was attributed to the high surface area of the catalysts, along with the synergism created by the silver nanoparticles and MoS2. All the catalysts were characterized by X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, field-emission scanning electron microscopy (FE-SEM), high-resolution transmission electron microscopy (HRTEM), Brunauer–Emmett–Teller (BET) surface area analysis and energy dispersive X-ray spectroscopy (EDS)