35 research outputs found
Bioeutectic® ceramics for biomedical application obtained by laser floating zone method. In vivo evaluation
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license.In this study, the Bioeutectic® blocks were inserted into the critical size defects of eight rabbits, using both tibiae, and the physical and chemical nature of the remodeled interface between the Bioeutectic® implants and the surrounding bone were performed at four and 15 months. The results showed a new fully mineralized bone growing in direct contact with the implants. The ionic exchange, taking place at the implant interface with the body fluids was essential in the process of the implant integration through a dissolution-precipitation-transformation mechanism. The study found the interface biologically and chemically active over the 15 months implantation period. The osteoblastic cells migrated towards the interface and colonized the surface at the contact areas with the bone. The new developed apatite structure of porous morphology mimics natural bone. © 2014 by the authors.The authors wish to acknowledge funding from the European Community at the 7th Framework Program EU No. 314630-UV Marking and Generalitat Valenciana (GVA) within the Project ACOMP/2009/173.Peer Reviewe
Respuesta ósea al implante del compuesto beta-fosfato tricálcico-colágeno (ß-ftc-c). Estudio «in vivo» en conejos
Se ha estudiado la correlación entre los hallazgos histológicos y las imágenes radiológicas
de un compuesto de beta-fosfato tricálcico y colágeno (ß-FTC-C) después de su implantación
en defectos óseos creados experimentalmente en 21 conejos de 4 Kg de peso
medio. Bajo anestesia general los defectos óseos creados en ambos fémures se rellenaron con
un cilindro de 4,5 x 6 mm del compuesto. Los animales fueron sacrificados a la semana y 1, 2,
4 y 12 meses del postoperatorio. Las muestras obtenidas fueron sometidas a estudio radiográfico
e histológico. Microscópicamente se comprobó que el compuesto a lo largo del período de
implantación sufrió un proceso de reabsorción progresiva mediado por células de estirpe macrofágica
y que se correspondió con una disminución de la densidad radiológica y cambios en
su morfología. A los doce meses del postoperatorio se observaron escasos restos del material,
hecho que se corresponde con la desaparición del implante en la placa radiográfica.The correlation between histologic and radiological findings was studied after
implantation of a composite (ß-FTC-C) into osseus defects which were experimentally induced
in 21 rabbits. Under general anaesthetic osseus defects were made in both femurs and filled
in with a 4,5 x 6 mm composite cylinder. The animals were killed at the first week and,
one, two, four and twelve months after surgery. X-ray study and microscopic examination
were made in all the specimens. Troughout the implantation period, it was microscopically
proven that the composite showed a progressive reabsortion process, mediated by cells of
macrophagus lineage. A decrease in radiologic density and changes in its morphology were
also observed. At the end of the study (twelve months), limited traces of the material could
be seen, in correlation with the images of the X-ray films which revealed the total absence of
the composite implant
Aislamiento y cultivo de células osteoblásticas: Interés para la investigación en cirugía ortopédica y traumatología
Describimos un método de aislamiento y caracterización de células osteoblásticas
procedentes de la superficie de trabéculas óseas de explantes obtenidos de metafisis de
fémures y tibias de conejo y de cabezas femorales humanas de pacientes sometidos a cirugía
protésica de cadera. Después de eliminar las células de la médula ósea de los explantes, estos
se despositaron sobre dos tipos de mallas, de 80 y 100 µm., obteniéndose un cultivo en
monocarpa que se mostró confluente a partir de los 20 días. La población celular aislada
presentó un fenotipo osteoblástico utilizando métodos colorimétricos para determinación de
la actividad de fosfatasa alcalina (FA) y morfológicos por medio de la observación con microscopía
electrónica de barrido. Las células cultivadas exhibieron una morfología diferente
según el grado de confluencia de cultivo, siendo en todo momento positivas las sucesivas determinaciones
colorimétricas de FA realizadas a lo largo de la experiencia. Consideramos el
método de gran interés en investigación en ortopédica, pues permite el estudio de comportamiento
de estas células cuando están sometidas a cualquier tipo de estímulo externo, así como
cuando se enfrentan a nuevas generaciones de biomateriales, etc.We describe a method of isolation and characterization of osteoblastic cells taken
from the surface of bone trabeculae of explants obtained from femur and tibia metaphysis
in rabbits and human femoral head in patients undergoing orthopaedic surgery. Afther removing
the cells from the bone marrow of explants, they were placed over two mesh, 80 and
100 µmn, obtaining then a culture in monolayer which showed to be confluent after 20 days.
The isolated cell population showed an osteoblastic phenotype using colourmetric methods to
determinate the alkaline phosphatase (AF) activity and morphologic methods by means of
scanning. The cultivated cell showed a different morphology according to the degree of confluence
of the culture, being always positive the sucesive colourmetric determinations of AF
along the experiment. We consider the method of a great interest for orthopaedic investigations,
since it allows the study of the behaviour of these cells when they are subjet to different
types of external stimulus as well as meet they the new generations of biomaterials
The Effect of Germanium‐Loaded Hydroxyapatite Biomaterials on Bone Marrow Mesenchymal Stem Cells Growth
Hydroxyapatite (HA) is a hard mineral component of mineralized tissues, mainly composed of calcium and phosphate. Due to its bioavailability, HA is potentially used for the repair and regeneration of mineralized tissues. For this purpose, the properties of HA are significantly improved by adding natural and synthetic materials. In this sense, the germanium (Ge) mineral was loaded in HA biomaterial by cold isostatic pressure for the first time and characterization and biocompatibility using bone marrow mesenchymal stem cells (BM-MSCs) were investigated. The addition of Ge at 5% improved the solubility (3.32%), stiffness (18.34 MPa), water holding (31.27%) and biodegradation (21.87%) properties of HA, compared to control. Compared to all composite biomaterials, the drug-releasing behavior of HA-3% Ge was higher at pH 1 and 3 and the maximum drug release was obtained at pH 7 and 9 with HA-5% Ge biomaterials. Among the different mediums tested, the DMEM-medium showed a higher drug release rate, especially at 60 min. HA-Ge biomaterials showed better protein adhesion and apatite layer formation, which ultimately proves the compatibility in BM-MSCs culture. Except for higher concentrations of HA (5 and 10 mg/mL), the different concentrations of Ge and HA and wells coated with 1% of HA-1% Ge had higher BM-MSCs growth than control. All these findings concluded that the fabricated HA biomaterials loaded with Ge could be the potential biomaterial for culturing mammalian cells towards mineralized tissue repair and regeneration.This research was funded by MCIN/AEI/10.13039/501100011033 Grant numbers PID2020-116693RB-C21 and PID2020-116693RB-C22Generalitat Valenciana Grant number CIAICO/2021/157and Internal Research Fund from Universidad Católica San Antonio de Murcia (UCAM), Grant number PMAFI-27/21, Murcia, Spain
Mechanical behaviour and formation process of silkworm silk gut
High performance silk fibers were produced directly from the silk glands of silkworms ("Bombyx mori") following an alternative route to natural spinning. This route is based on a traditional procedure that consists of soaking the silk glands in a vinegar solution and stretching them by hand leading to the so called silkworm guts. Here we present, to the authors’ best knowledge, the first comprehensive study on the formation, properties and microstructure of silkworm gut fibers. Comparison of the tensile properties and microstructural organization of the silkworm guts with those of naturally spun fibers allows gain of a deeper insight into the mechanisms that lead to the formation of the fiber, as well as the relationship between the microstructure and properties of these materials. In this regard, it is proved that an acidic environment and subsequent application of tensile stress in the range of 1000 kPa are sufficient conditions for the formation of a silk fiber
Estudio morfologico de la respuesta osea inducida por la ceramica #beta#-forfato tricalcico y el composite #beta#-fosfato tricalcico-colageno Modelo experimental en conejo
Available from Centro de Informacion y Documentacion Cientifica CINDOC. Joaquin Costa, 22. 28002 Madrid. SPAIN / CINDOC - Centro de Informaciòn y Documentaciòn CientìficaSIGLEESSpai
Estudio morfológico de la respuesta ósea inducida por la cerámica B- fosfato tricálcico y el compositen B-fosfato tricálcico-colágeno : modelo experimental en conejo / Luis R. Meseguer Olmo; directores Manuel Clavel-Sainz Nolla, Vicente Vicente Ortega.
Tesis-Universidad de Murcia.MEDICINA ESPINARDO. DEPOSITO. MU-Tesis 318.Consulte la tesis en: BCA. GENERAL. ARCHIVO UNIVERSITARIO. T.M.-909
Estudio morfológico de la respuesta ósea inducida por la cerámica beta-FTC y el composite beta-FTC-colágeno [Microforma]: Modelo experimental en conejo.
Tesis-Universidad de Murcia.Consulte la tesis en: BCA. GENERAL. MEDIATECA. MF-TD 3224.Consulte la tesis en: BCA. GENERAL. MEDIATECA. MF-TD 1678
Human mesenchymalstemcellviability,proliferationanddifferentiation potential inresponsetoceramicchemistryandsurfaceroughness
We investigatedtheeffectoftheceramicchemistryandsurfaceroughnessofpure α-tricalcium phosphate,andalso αTCP dopedwitheither
1.5 wt%or3.0wt%ofdicalciumsilicate(C2S), ontheresponseofadulthumanmesenchymalstemcells(ahMSCs). AhMSCs wereplatedonto
ceramic discs,preparedbyasolid-statereaction.Afterbeingsintered,somesampleswerepolishedupto1 μm, whileotherswerekeptas
manufactured, whichresultedintwosurfaceroughnessgrades.Viability,proliferationandosteoinductivecapacityweredeterminedfollowing
various incubationperiods.
The resultsshowedanon-cytotoxiceffectafteranindirectapoptosistest.Celladhesionandproliferationweresurfaceroughness-sensitiveand
increased proportionallytotheroughnessofmaterials.Theseobservationsbecamemoreevidentintheunpolished αTCP ceramicdopedwith
1.5 wt%C2S, whichinducedosteoblasticdifferentiationasaresultoftheroughnessandincreasedconcentrationoftheC2S solidsolution
in αTCP
Diferenciación fibroblástica bajo la influencia de la proteína morfogenética ósea (rh-BMP-2) : Estudio sobre dos poblaciones fibroblásticas, piel y tendón / Emilio José Salmerón Martínez ; directores Luis R. Meseguer Olmo, José Muñoz Ramos.
Tesis-Universidad de Murcia.MEDICINA ESPINARDO. DEPOSITO. MU-Tesis 637.Consulte la tesis en: BCA. GENERAL. DEPOSITO. T.M-1964